3,804 research outputs found

    Food technology neophobia and consumer attitudes toward foods produced by new and conventional technologies: A case study in Brazil

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    AbstractNew food technologies are promoting innovations in the food sector. However, not all technologies are accepted and understood by consumers; some cause resistance. The present work sought to study the behavior of Brazilian consumers in relation to different food technologies. A questionnaire was administered to a representative sample of 389 respondents in Belo Horizonte-MG, Brazil. Questionnaire collected information on consumer perceptions regarding new technologies by means of the Food Technology Neophobia Scale (FTNS), translated and validated into Portuguese, in addition to familiarity and willingness to try yogurts labeled such as traditional, pasteurized, organic, genetically modified, enriched with bioactive proteins and nanotechnology. Results suggested that neophobia regarding food technology is important to explain consumer behavior in relation to new technologies, especially for nanotechnology. Participants were less familiar with foods labeled as GM and nanotechnology, and willingness to try these products was lower. Consumers are still wary of GM and nanotechnology, possibly due to lack of assurance that these foods are safe for human health and the environment. For new food technologies (such as nanotechnology) that are still recent, communication is very important, being decisive for the consolidation of consumer perceptions, and consequently for the acceptance of these innovations on the market

    Inhibitory action of Lippia gracilis Schauer essential oil on pathogenic bacteria and its effects as a growth promoter on quail

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    Aim of the study: To examine the in vitro sensitivity of Salmonella sp. and Escherichia coli strains to the microbial activity of Lippia gracilis Schauer essential oil (LGSEO) and to determine the optimal level of LGSEO as a growth promoter in diets for Japanese quail up to 35 days of age.Area of study: São Cristovão, Sergipe, Brazil.Material and methods: A total of 504 female Japanese quails (Coturnix coturnix japonica) at an initial average body weights of 6.80±0.10 g was allotted to one of six treatments (0, 100, 200, 300, 400 mg/kg of LGSEO and a diet containing 500 mg/kg of bacitracin methylene disalicylate) in 7 replicates, using 12 birds per experimental unit.Main results: In the age period of 21 to 35 days, feed intake declined linearly (p=0.04) and feed efficiency improved (p<0.01), whereas no changes were observed in production performance (p>0.05). The estimated (p=0.01) maximum relative weights of proventriculus and pancreas were obtained at the LGSEO inclusion levels of 196.5 and 251 mg/kg, respectively. Inclusion of 100 to 300 mg/kg of LGSEO in the diet reduced the total Salmonella sp. bacterial count.Research highlights: The use of 196.5 mg/kg of LGSEO in the diet of Japanese quail improved production performance and organ development and demonstrated potential antimicrobial capacity against Salmonella sp. bacteria. Due its pharmacological composition, LGSEO can potentially substitute to antimicrobials, because contains thymol and carvacrol as main active constituents

    Electroanalysis of Imidacloprid Insecticide in River Waters Using Functionalized Multi-Walled Carbon Nanotubes Modified Glassy Carbon Electrode

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    In this work, a functionalized multi-walled carbon nanotubes modified glassy carbon electrode (GCE/MWCNT-f) was optimized for the direct determination of imidacloprid (IMC) insecticide in river water. The functionalized material was characterized by infrared spectroscopy with Fourier transform (FTIR) and the modified electrode by scanning electron microscopy (SEM) and cyclic voltammetry (CV). Results revealed that the GCE/MWCNT-f effectively increased the response toward IMC reduction by enhancing the reduction peak current and decreasing the peak potential in comparison with the bare electrode. After optimizing the electroanalytical conditions, the GCE/MWCNT-f showed a linear voltammetric response at concentration ranging from 2.40 × 10−7 to 3.50 × 10−6 mol L−1, with detection and quantification limits of 4.15 × 10−7 mol L−1 and 1.38 × 10−6 mol L−1, respectively. The recovery rate of IMC in spiked river water samples varied from 90–95%. Thus, this sensor can be a promising tool for the analysis and monitoring of IMC in complex environmental matrices.info:eu-repo/semantics/publishedVersio

    Agronomic performance of interspecific Paspalum hybrids under nitrogen fertilization or mixed with legumes

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    Nitrogen supply and mixtures with legumes affect agronomic performance of pas- tures, and both practices can guide breeding decisions in Paspalum hybrids. The goals of this study were: (a) quantify herbage accumulation (HA), leaf accumulation (LA), cold tolerance, and N use efficiency (NUE) in P. plicatulum × P. guenoarum hybrids subjected to N fertilization or grown in a mixture with legumes; (b) compare the grass–legume system to a grass–N fertilizer system; and (c) select the best hybrid for future cultivar releases. A randomized complete block design with three repli- cations and a split-plot treatment arrangement was used for 2 yr, with five N rates (0, 60, 120, 240, and 480 kg N ha−1) and a grass–legume mixture [grass + white clover (Trifolium repens L.) + birdsfoot trefoil (Lotus corniculatus L.)] as whole plots, and six genotypes as subplots (hybrids: 1020133, 102069, 103084, 103061; and controls: P. guenoarum ‘Azulão’ and Megathyrsus maximus ‘Aruana’). Higher N rates increased HA, LA, and cold tolerance. Higher NUE was obtained between 60 and 120 kg N ha−1. In the grass–legume mixture HA was similar to the rates of 60 and 120 kg N ha−1. Hybrid 1020133 had HA similar to the controls, LA greater than Aruana, and greater cold tolerance and NUE at 60 kg N ha−1 than Azulão and Aruana. Hybrid 1020133 should be selected for further animal performance stud- ies. The agronomic performance of perennial pastures can be improved through N management, and NUE should be a selection criterion in forage breeding

    The frequency of CD127low expressing CD4+CD25high T regulatory cells is inversely correlated with human T lymphotrophic virus type-1 (HTLV-1) proviral load in HTLV-1-infection and HTLV-1-associated myelopathy/tropical spastic paraparesis

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    <p>Abstract</p> <p>Background</p> <p>CD4<sup>+</sup>CD25<sup>high </sup>regulatory T (T<sub>Reg</sub>) cells modulate antigen-specific T cell responses, and can suppress anti-viral immunity. In HTLV-1 infection, a selective decrease in the function of T<sub>Reg </sub>cell mediated HTLV-1-tax inhibition of FOXP3 expression has been described. The purpose of this study was to assess the frequency and phenotype of T<sub>Reg </sub>cells in HTLV-1 asymptomatic carriers and in HTLV-1-associated neurological disease (HAM/TSP) patients, and to correlate with measures of T cell activation.</p> <p>Results</p> <p>We were able to confirm that HTLV-I drives activation, spontaneous IFNγ production, and proliferation of CD4+ T cells. We also observed a significantly lower proportion of CTLA-4<sup>+ </sup>T<sub>Reg </sub>cells (CD4<sup>+</sup>CD25<sup>high </sup>T cells) in subjects with HAM/TSP patients compared to healthy controls. Ki-67 expression was negatively correlated to the frequency of CTLA-4<sup>+ </sup>T<sub>Reg </sub>cells in HAM/TSP only, although Ki-67 expression was inversely correlated with the percentage of CD127<sup>low </sup>T<sub>Reg </sub>cells in healthy control subjects. Finally, the proportion of CD127<sup>low </sup>T<sub>Reg </sub>cells correlated inversely with HTLV-1 proviral load.</p> <p>Conclusion</p> <p>Taken together, the results suggest that T<sub>Reg </sub>cells may be subverted in HAM/TSP patients, which could explain the marked cellular activation, spontaneous cytokine production, and proliferation of CD4<sup>+ </sup>T cells, in particular those expressing the CD25<sup>high</sup>CD127<sup>low </sup>phenotype. T<sub>Reg </sub>cells represent a potential target for therapeutic intervention for patients with HTLV-1-related neurological diseases.</p

    A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers

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    The development of sugarcane as a sustainable crop has unlimited applications. The crop is one of the most economically viable for renewable energy production, and CO2 balance. Linkage maps are valuable tools for understanding genetic and genomic organization, particularly in sugarcane due to its complex polyploid genome of multispecific origins. The overall objective of our study was to construct a novel sugarcane linkage map, compiling AFLP and EST-SSR markers, and to generate data on the distribution of markers anchored to sequences of scIvana_1, a complete sugarcane transposable element, and member of the Copia superfamily. The mapping population parents (‘IAC66-6’ and ‘TUC71-7’) contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of co-segregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19 cM, with a marker density of 8.87 cM. Markers were assembled into 92 CGs that ranged in length from 1.14 to 404.72 cM, with an estimated average length of 52.64 cM. The greatest distance between two adjacent markers was 48.25 cM. The scIvana_1-based markers (56) were positioned on 21 CGs, but were not regularly distributed. Interestingly, the distance between adjacent scIvana_1-based markers was less than 5 cM, and was observed on five CGs, suggesting a clustered organization. Results indicated the use of a NBS-profiling technique was efficient to develop retrotransposon-based markers in sugarcane. The simultaneous maximum-likelihood estimates of linkage and linkage phase based strategies confirmed the suitability of its approach to estimate linkage, and construct the linkage map. Interestingly, using our genetic data it was possible to calculate the number of retrotransposon scIvana_1 (~60) copies in the sugarcane genome, confirming previously reported molecular results. In addition, this research possibly will have indirect implications in crop economics e.g., productivity enhancement via QTL studies, as the mapping population parents differ in response to an important fungal disease13CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPnão temnão tem2010/51708-

    A novel experimental procedure for lock-in thermography on solar cells

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    The occurrence of defects in solar cells is intrinsically related to a reduction in the efficiency and reliability of these devices. Therefore, monitoring techniques, such as lock-in thermography, electroluminescence and the I-V characteristic curve are adopted in order to evaluate the integrity of the solar cells. In the present work, a novel experimental procedure for the lock-in thermography of solar cells is proposed, aiming to improve the detection capability of the assay. Conventional techniques use pulse width modulation to operate the cell at a fixed point on the I-V curve. Instead, we propose a methodology based on a sinusoidal electric current excitation in order to extend the range of operational points that are close to the maximum power point as the cell operates in the field. Some traditional image processing techniques (principal component analysis, the fast Fourier transform and the four-step phase-shifting method) have been used to analyze the thermal images captured by an infrared camera during steady-state operation mode of the solar cells using both sinusoidal electric current signal and standard pulse width modulation procedures. Comparison between the results of both procedures found that this novel approach provides smoother and clearer delimitation of the defects. Furthermore, the contrast of the phase images was found to exhibit significant changes between the defective and non-defective regions for different modulation frequencies and types of defects. From the achieved results, it was possible to obtain a satisfactory characterization of the existing defects
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