The mediterranean biotypes of Tylenchulus semipenetrans in Spanish citrus orchards

Les capacités de reproduction de quatorze populations du nématode des citrus (#Tylenchulus semipenetrans$) collectées dans la principale zone de citriculture de l'Espagne ont été comparées sur oranger amer et citrange Carrizo. Toutes les populations ont été identifiées comme appartenant au biotype méditerranéen. La capacité de reproduction varie considérablement en fonction des populations. Le pouvoir infestant et le taux de reproduction des populations collectées dans la rhizosphère des porte-greffes citranges Troyer ou Carrizo sont plus élevés (P supérieur ou égal à 0,05) que ceux des populations collectées sur oranger amer. (Résumé d'auteur

TEMPORAL AND SPATIAL VARIABILITY OF ATMOSPHERIC PARTICLE NUMBER SIZE DISTRIBUTIONS ACROSS SPAIN

This study synthesizes for the first time results from simultaneous aerosol measurements performed at seven diverse locations distributed all over the Spanish geography. The observations were carried out during two field campaigns in 2012–2013, one-month each and during different seasons. These field campaigns were performed in the framework of the Spanish Network of DMAs (REDMAAS) activities. Measurement sites were grouped as polluted sites (urban background) and clean sites (rural background and high-altitude sites). Seasonal differences were more important at polluted sites, mainly related to meteorology and aerosol sources. Higher total particle concentrations were found during the cold period, driven mainly by Aitken-mode particles (traffic-related aerosol particles).This work has been financed by the Ministry of Science and Innovation (CGL2011-15008-E, CGL2010-1777, CGL2011-27020, CGL2014-52877-R, CGL2010-11095-E, CGL2012-39623-C02-01, CGL2014-55230-R & PI15/0051

A long-term study of new particle formation in a coastal environment: Meteorology, gas phase and solar radiation implications

New particle formation (NPF) was investigated at a coastal background site in Southwest Spain over a four-year period using a Scanning Particle Mobility Sizer (SMPS). The goals of the study were to characterise the NPF and to investigate their relationship to meteorology, gas phase (O3, SO2, CO and NO2) and solar radiation (UVA, UVB and global). A methodology for identifying and classifying the NPF was implemented using the wind direction and modal concentrations as inputs. NPF events showed a frequency of 24% of the total days analyzed. The mean duration was 9.2±4.2 hours. Contrary to previous studies conducted in other locations, the NPF frequency reached its maximum during cold seasons for approximately 30% of the days. The lowest frequency took place in July with 10%, and the seasonal wind pattern was found to be the most important parameter influencing the NPF frequency. The mean formation rate was 2.2±1.7 cm-3 s-1, with a maximum in the spring and early autumn and a minimum during the summer and winter. The mean growth rate was 3.8±2.4 nm h-1 with higher values occurring from spring to autumn. The mean and seasonal formation and growth rates are in agreement with previous observations from continental sites in the Northern Hemisphere. NPF classification of different classes was conducted to explore the effect of synoptic and regional-scale patterns on NPF and growth. The results show that under a breeze regime, the temperature indirectly affects NPF events. Higher temperatures increase the strength of the breeze recirculation, favouring gas accumulation and subsequent NPF appearance. Additionally, the role of high relative humidity in inhibiting the NPF was evinced during synoptic scenarios. The remaining meteorological variables (RH), trace gases (CO and NO), solar radiation, PM10 and condensation sink, showed a moderate or high connection with both formation and growth rates.This work was partially supported by the Andalusian Regional Government through projects P10-RNM-6299 and P12-RNM-2409, the Spanish Ministry of Science and Technology (MINECO) through projects CGL2010-18782, CGL2011-24891/CLI, CGL2013-45410-R and the Complementary Action CGL2011-15008-E.European Union through the ACTRIS project (EU INFRA-2010-1.1.16-262254)

Evaluación de filtrados de cultivos fúngicos de hongos por su actividad frente a Tylenchulus semipenetrans

Culture filtrates of 20 fungi isolated from citrus soils were screened for their activity against Tylenchulus semipenetrans in both in vitro and greenhouse tests. The filtrates of Talaromyces cyanescens (isolates 2-4 and 2-5), Paecilomyces lilacinus, Chaetomium robustum, Acremonium strictum, Engyodontium album, Myrothecium verrucaria, Emericella rugulosa, and Tarracomyces gigaspora consistently inhibited the motility of second-stage juveniles at various concentrations of the filtrate. Dose-response models were used to determine the filtrate concentration required to inhibit the motility of 50% of the juveniles (CI50). The culture filtrate of P. lilacinus showed the highest activity with a CI50 value of 58% that differed from that of C. robustum (CI50 = 68%), and A. strictum CI50 = 82%. The culture filtrates of P. lilacinus, E. album, and T. cyanescens 2-5 maintained their activity when autoclaved at 120 deg C for 20 min. The autoclaved filtrate of T. cyanescens 2-4 was more effective at inhibiting juvenile motility (CI50 = 28%) than that of T. cyanescens 2-5 (CI50 = 80%), C. robustum (CI50 = 72 %) and P. lilacinus (CI50 = 72%). The culture filtrate of T. cyanescens 2-4 also inhibited egg hatching. Nematode reproduction on Cleopatra mandarin and Carrizo citrange were respectively reduced by the culture filtrate of P. lilacinus and the autoclaved filtrate of T. cyanescens 2-4. These results support the hypothesis that soil fungi may contribute to regulate nematode densities by the production of secondary metabolites with nematicidal activity.Se evaluó la actividad de filtrados de cultivos de 20 hongos aislados del suelo en parcelas de cítricos frente a Tylenchulus semipenetrans mediante ensayos in vitro y en invernadero. Los filtrados de Talaromyces cyanescens (aislados 2-4 y 2-5), Paecilomyces lilacinus, Chaetomium robustum, Acremonium strictum, Engyodontium album, Myrothecium verrucaria, Emericella rugulosa, y Tarracomyces gigaspora inhibieron consistentemente la movilidad de los juveniles de segundo estadio del nematodo a varias concentraciones del filtrado. Se utilizaron modelos de dosis-respuesta para determinar la concentración del filtrado que inhibía la movilidad del 50% de los juveniles (CI50). El filtrado del cultivo de P. lilacinus mostró la máxima actividad entre todos los ensayados con un valor de CI50 del 58%, la cual era superior a la de los filtrados de C. robustum (CI50 = 68%) y A. strictum (CI50 = 82%). Los filtrados de P. lilacinus, E. album, y T. cyanescens 2-5 retenían su actividad después de autoclavarlos a 120 grados C durante 20 min. La inhibición de la movilidad de los juveniles obtenida con el filtrado autoclavado de T. cyanescens 2-4 (CI50 = 28%) fue superior a la de los filtrados de T. cyanescens 2-5 (CI50 = 80%), C. robustum (CI50 = 72%), ó P. lilacinus (CI50 = 72%). El filtrado de T. cyanescens 2-4 también inhibió la eclosión de los huevos del nematodo. La reproducción del nematodo en mandarino Cleopatra y citrange Carrizo fue menor cuando los juveniles se incubaron en el filtrado de P. lilacinus y en el filtrado autoclavado T. cyanescens 2-4, respectivamente. Estos resultados apoyan la hipótesis de que los hongos del suelo pueden contribuir a la regulación de las poblacionales de los nematodos mediante la producción de metabolitos secundarios con acción nematicida

The mediterranean biotypes of Tylenchulus semipenetrans in Spanish citrus orchards

Les capacités de reproduction de quatorze populations du nématode des citrus (#Tylenchulus semipenetrans$) collectées dans la principale zone de citriculture de l'Espagne ont été comparées sur oranger amer et citrange Carrizo. Toutes les populations ont été identifiées comme appartenant au biotype méditerranéen. La capacité de reproduction varie considérablement en fonction des populations. Le pouvoir infestant et le taux de reproduction des populations collectées dans la rhizosphère des porte-greffes citranges Troyer ou Carrizo sont plus élevés (P supérieur ou égal à 0,05) que ceux des populations collectées sur oranger amer. (Résumé d'auteur

Minimally invasive system to reliably characterize ventricular electrophysiology from living donors

Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratome-sliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verified viability. Optically mapped transmembrane potentials confirmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and ß-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically