Comparison of two different indentation techniques in studying the in-situ viscoelasticity behavior of liquid crystals

YesLiquid crystal is a new emerging biomaterial. The physical property of liquid crystal plays a role in supporting the adhesion of cells. Nano and microball indentation techniques were applied to determine the elastic modulus or viscoelasticity of the cholesteryl ester liquid crystals in the culture media. Nano-indentation results (108 ± 19.78 kPa, N = 20) agreed well with the microball indentation (110 ± 19.95 kPa, N = 60) for the liquid crystal samples incubated for 24 hours at 37o C, respectively. However, nanoindentation could not measure the modulus of the liquid crystal (LC) incubated more than 24 hours. This is due to the decreased viscosity of the liquid crystal after immersion in the cell culture media for more than 24 hours. Alternatively, microball indentation was used and the elastic modulus of the LC immersed for 48 hours was found to decrease to 55 ± 9.99 kPa (N = 60). The microball indentation indicated that the LC did not creep after 40 seconds of indentation. However, the elastic modulus of the LC was no longer measurable after 72 hours of incubation due to the lost of elasticity. Microball indentation seemed to be a reliable technique in determining the elastic moduli of the cholesteryl ester liquid crystals.Science Fund Vot. No. S024 or Project No. 02- 01-13-SF0104 and FRGS Vot. No. 1482 awarded by Malaysia Ministry of Educatio

Electrocardiograph (ECG) circuit design and software-based processing using LabVIEW

YesThe efficiency and acquisition of a clean (diagnosable) ECG signal dependent upon the proper selection of electronic components and the techniques used for noise elimination. Given that the human body and the lead cables act as antennas, hence picking up noises from the surroundings, thus a major part in the design of an ECG device is to apply various techniques for noise reduction at the early stage of the transmission and processing of the signal. This paper, therefore, covers the design and development of a Single Chanel 3-Lead Electrocardiograph and a Software-based processing environment. Main design characteristics include reduction of common mode voltages, good protection for the patient, use of the ECG device for both monitoring and automatic extraction (measurements) of the ECG components by the software. The hardware consisted of a lead selection stage for the user to select the bipolar lead for recording, a pre-amplification stage for amplifying the differential potentials while rejecting common mode voltages, an electrical isolation stage from three filtering stages with different bandwidths for noise attenuation, a power line interference reduction stage and a final amplification stage. A program in LabVIEW was developed to further improve the quality of the ECG signal, extract all its features and automatically calculate the main ECG output waveforms. The program had two main sections: The filtering section for removing power line interference, wideband noises and baseline wandering, and the analysis section for automatically extracting and measuring all the features of the ECG in real time. A Front Panel Environment was, therefore, developed for the user interface. The present system produced ECG tracings without the influence of noise/artefacts and provided accurate detection and measurement of all the components of the ECG signal

Electrocardiograph (ECG) circuit design and software-based processing using LabVIEW

The efficiency and acquisition of a clean (diagnosable) ECG signal dependent upon the proper selection of electronic components and the techniques used for noise elimination. Given that the human body and the lead cables act as antennas, hence picking up noises from the surroundings, thus a major part in the design of an ECG device is to apply various techniques for noise reduction at the early stage of the transmission and processing of the signal. This paper, therefore, covers the design and development of a Single Chanel 3-Lead Electrocardiograph and a Software-based processing environment. Main design characteristics include reduction of common mode voltages, good protection for the patient, use of the ECG device for both monitoring and automatic extraction (measurements) of the ECG components by the software. The hardware consisted of a lead selection stage for the user to select the bipolar lead for recording, a pre-amplification stage for amplifying the differential potentials while rejecting common mode voltages, an electrical isolation stage from three filtering stages with different bandwidths for noise attenuation, a power line interference reduction stage and a final amplification stage. A program in LabVIEW was developed to further improve the quality of the ECG signal, extract all its features and automatically calculate the main ECG output waveforms. The program had two main sections: The filtering section for removing power line interference, wideband noises and baseline wandering, and the analysis section for automatically extracting and measuring all the features of the ECG in real time. A Front Panel Environment was, therefore, developed for the user interface. The present system produced ECG tracings without the influence of noise/artefacts and provided accurate detection and measurement of all the components of the ECG signal

In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique

YesCells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.Malaysia Ministry of Education (Fundamental Research Grant Scheme, FRGS Vot. 1482 and IGSP Vot. 679)

In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique

YesCells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.Malaysia Ministry of Education (Fundamental Research Grant Scheme, FRGS Vot. 1482 and IGSP Vot. 679)

Dye-sensitized solar cell using pure anatase TiO2 annealed at different temperatures

The performance of pure anatase titanium dioxide (TiO2) annealed at different tempera-tures as photoanode in the application of dye-sensitized solar cell (DSSC) was investigated and discussed. All samples of TiO2 were deposited on fluorine-doped tin oxide (SnO2) on glass substrate using spray pyrolysis deposition (SPD) method. Characterizations of the DSSCs fabricated were executed on their surface morphology, structural property, and energy conversion efficiency. In the DSSC preparation, anatase TiO2 thin films, platinum (Pt), ruthenium-based dye N719 and DPMII triiodide couple electrolyte were used as pho-toanodes, cathode/counter electrode, dye sensitizers and liquid electrolyte, respectively. All of the TiO2 photoanodes were annealed at 300 ◦C, 400 ◦C and 500 ◦C with a set left without any heat treatment. The thickness of anatase TiO2 photoanodes measured were in between 23 �m and 41 �m. The power conversion efficiency of DSSCs performed under visible light with intensity of 100 mW/cm2 shows that DSSC with pure anatase phased TiO2 annealed at 500 ◦C as photoanode yields the highest efficiency of 3.25%

Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques

NoGrowing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 μm and 141 ± 70 μm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications.(Science Fund Vot No.: 0201-01-13-SF0104 or S024) awarded by Malaysia Ministry of Science and Technology (MOSTI) and IGSP Grant Vot No. U679 awarded by Universiti Tun Hussein Onn Malaysia

A pilot study to examine the correlation between cognition and blood biomarkers in a Singapore Chinese male cohort with type 2 diabetes mellitus

10.1371/journal.pone.0096874PLoS ONE95e9687

Development of a novel liquid crystal based cell traction force transducer system

Keratinocyte traction forces play a crucial role in wound healing. The aim of this study was to develop a novel cell traction force (CTF) transducer system based on cholesteryl ester liquid crystals (LC). Keratinocytes cultured on LC induced linear and isolated deformation lines in the LC surface. As suggested by the fluorescence staining, the deformation lines appeared to correlate with the forces generated by the contraction of circumferential actin filaments which were transmitted to the LC surface via the focal adhesions. Due to the linear viscoelastic behavior of the LC, Hooke's equation was used to quantify the CTFs by associating Young's modulus of LC to the cell induced stresses and biaxial strain in forming the LC deformation. Young's modulus of the LC was profiled by using spherical indentation and determined at approximately 87.1±17.2. kPa. A new technique involving cytochalasin-B treatment was used to disrupt the intracellular force generating actin fibers, and consequently the biaxial strain in the LC induced by the cells was determined. Due to the improved sensitivity and spatial resolution (~1μm) of the LC based CTF transducer, a wide range of CTFs was determined (10-120. nN). These were found to be linearly proportional to the length of the deformations. The linear relationship of CTF-deformations was then applied in a bespoke CTF mapping software to estimate CTFs and to map CTF fields. The generated CTF map highlighted distinct distributions and different magnitude of CTFs were revealed for polarized and non-polarized keratinocytes. © 2012 Elsevier B.V.