Samsungs raumgestalterische Tätigkeit in Seoul. Eine Zustandsanalyse des Quartiers im Bereich der Unternehmenszentrale von Samsung

Die Samsung Group ist das größte koreanische Chaebol, welches auch das Bauunternehmen Samsung C&T Engineering Construction Group, zu dem u.a. Raemian gehört, mit einschließt (Andolfo 2018, S. 59). Da es starke Verflechtungen zwischen den Chaebols und der südkoreanischen Politik gibt, erscheint es naheliegend, dass Samsung nun nicht nur als Unternehmen auftritt, sondern auch als aktiver Akteur in der Raumgestaltung. Zu vermuten ist, dass das Unternehmen dabei eigene Interessen verfolgt. Für eine Untersuchung dieser Thematik steht der Bereich nahe der Unternehmenszentrale von Samsung, die sogenannte Samsung Town, in Seoul im Fokus. Welche Aussagen lassen sich über den Zustand dieses Quartiers im Hinblick auf die raumgestalterischen Tätigkeiten Samsungs treffen? Diese Fragestellung wird mit Hilfe einer systematischen Beobachtung beantwortet. Als theoretische Grundlage dient die Quartiersforschung, wobei die Nachbarschaftsforschung und das Konzept der Urban Governance als Zugang zum Quartier genutzt werden. Über die verwendete Theorie wird im Folgenden ein Überblick gegeben, ebenso über die verwendete Methode und das genaue Vorgehen. Im Anschluss werden die empirischen Forschungsergebnisse dargelegt und eine Diskussion eingeleitet

Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

BACKGROUND: Coxiella burnetii, the bacterium causing Q fever, is an obligate intracellular biosafety level 3 agent. Detection and quantification of these bacteria with conventional methods is time consuming and dangerous. During the last years, several PCR based diagnostic assays were developed to detect C. burnetii DNA in cell cultures and clinical samples. We developed and evaluated TaqMan-based real-time PCR assays that targeted the singular icd (isocitrate dehydrogenase) gene and the transposase of the IS1111a element present in multiple copies in the C. burnetii genome. RESULTS: To evaluate the precision of the icd and IS1111 real-time PCR assays, we performed different PCR runs with independent DNA dilutions of the C. burnetii Nine Mile RSA493 strain. The results showed very low variability, indicating efficient reproducibility of both assays. Using probit analysis, we determined that the minimal number of genome equivalents per reaction that could be detected with a 95% probability was 10 for the icd marker and 6.5 for the IS marker. Plasmid standards with cloned icd and IS1111 fragments were used to establish standard curves which were linear over a range from 10 to 10(7 )starting plasmid copy numbers. We were able to quantify cell numbers of a diluted, heat-inactivated Coxiella isolate with a detection limit of 17 C. burnetii particles per reaction. Real-time PCR targeting both markers was performed with DNA of 75 different C. burnetii isolates originating from all over the world. Using this approach, the number of IS1111 elements in the genome of the Nine Mile strain was determined to be 23, close to 20, the number revealed by genome sequencing. In other isolates, the number of IS1111 elements varied widely (between seven and 110) and seemed to be very high in some isolates. CONCLUSION: We validated TaqMan-based real-time PCR assays targeting the icd and IS1111 markers of C. burnetii. The assays were shown to be specific, highly sensitive and efficiently reproducible. Cell numbers in dilutions of a C. burnetii isolate were reliably quantified. PCR quantification suggested a high variability of the number of IS1111 elements in different C. burnetii isolates, which may be useful for further phylogenetic studies

Технико-экономические и экологические аспекты утилизация шламов, содержащих соединения цинка

По причине токсичности перед сбросом в водоемы ионы цинка выделяются из сточных вод в виде твердых соединений, которые осаждаются в виде шлама в прудах-накопителях. Приведены данные по количественному и качественному составам шлама в накопителеотстойнике предприятия по производству вискозного волокна. Отмечено, что такой накопитель в г. Красноярске является техногенным образованием содержащем ценное сырье - цинк. Поэтому необходима разработка и внедрение технологий переработки и утилизации цинксодержащих шламов различных производств.Zinc ions are toxic and they are isolated from sewage in the form of solid compounds before discharge into water bodies. Precipitation occurs in the form of slime in storage ponds. Data on the quantitative and qualitative composition of the sludge in the reservoir-settler of an enterprise for the production of viscose fiber are given. It is noted that such a storage facility in Krasnoyarsk is a technogenic for mation containing valuable raw materials - zinc. Therefore, it is necessary to develop and implement technologies for processing and utilization of zinc-containing slurries of various industries

NFDI4Cat: Local and overarching data infrastructures

publishedVersio

A Unified Research Data Infrastructure for Catalysis Research – Challenges and Concepts

Modern research methods produce large amounts of scientifically valuable data. Tools to process and analyze such data have advanced rapidly. Yet, access to large amounts of high‐quality data remains limited in many fields, including catalysis research. Implementing the concept of FAIR data (Findable, Accessible, Interoperable, Reusable) in the catalysis community would improve this situation dramatically. The German NFDI initiative (National Research Data Infrastructure) aims to create a unique research data infrastructure covering all scientific disciplines. One of the consortia, NFDI4Cat, proposes a concept that serves all aspects and fields of catalysis research. We present a perspective on the challenging path ahead. Starting out from the current state, research needs are identified. A vision for a integrating all research data along the catalysis value chain, from molecule to chemical process, is developed. Respective core development topics are discussed, including ontologies, metadata, required infrastructure, IP, and the embedding into research community. This Concept paper aims to inspire not only researchers in the catalysis field, but to spark similar efforts also in other disciplines and on an international level.DFG, 441926934, NFDI4Cat – NFDI für Wissenschaften mit Bezug zur Katalys

A Unified Research Data Infrastructure for Catalysis Research – Challenges and Concepts

Modern research methods produce large amounts of scientifically valuable data. Tools to process and analyze such data have advanced rapidly. Yet, access to large amounts of high-quality data remains limited in many fields, including catalysis research. Implementing the concept of FAIR data (Findable, Accessible, Interoperable, Reusable) in the catalysis community would improve this situation dramatically. The German NFDI initiative (National Research Data Infrastructure) aims to create a unique research data infrastructure covering all scientific disciplines. One of the consortia, NFDI4Cat, proposes a concept that serves all aspects and fields of catalysis research. We present a perspective on the challenging path ahead. Starting out from the current state, research needs are identified. A vision for a integrating all research data along the catalysis value chain, from molecule to chemical process, is developed. Respective core development topics are discussed, including ontologies, metadata, required infrastructure, IP, and the embedding into research community. This Concept paper aims to inspire not only researchers in the catalysis field, but to spark similar efforts also in other disciplines and on an international level. © 2021 The Authors. ChemCatChem published by Wiley-VCH Gmb

Exorcising Grice’s ghost : an empirical approach to studying intentional communication in animals

Language’s intentional nature has been highlighted as a crucial feature distinguishing it from other communication systems. Specifically, language is often thought to depend on highly structured intentional action and mutual mindreading by a communicator and recipient. Whilst similar abilities in animals can shed light on the evolution of intentionality, they remain challenging to detect unambiguously. We revisit animal intentional communication and suggest that progress in identifying analogous capacities has been complicated by (i) the assumption that intentional (that is, voluntary) production of communicative acts requires mental-state attribution, and (ii) variation in approaches investigating communication across sensory modalities. To move forward, we argue that a framework fusing research across modalities and species is required. We structure intentional communication into a series of requirements, each of which can be operationalised, investigated empirically, and must be met for purposive, intentionally communicative acts to be demonstrated. Our unified approach helps elucidate the distribution of animal intentional communication and subsequently serves to clarify what is meant by attributions of intentional communication in animals and humans

Genome Sequencing of SHH Medulloblastoma Predicts Genotype-Related Response to Smoothened Inhibition

SummarySmoothened (SMO) inhibitors recently entered clinical trials for sonic-hedgehog-driven medulloblastoma (SHH-MB). Clinical response is highly variable. To understand the mechanism(s) of primary resistance and identify pathways cooperating with aberrant SHH signaling, we sequenced and profiled a large cohort of SHH-MBs (n = 133). SHH pathway mutations involved PTCH1 (across all age groups), SUFU (infants, including germline), and SMO (adults). Children >3 years old harbored an excess of downstream MYCN and GLI2 amplifications and frequent TP53 mutations, often in the germline, all of which were rare in infants and adults. Functional assays in different SHH-MB xenograft models demonstrated that SHH-MBs harboring a PTCH1 mutation were responsive to SMO inhibition, whereas tumors harboring an SUFU mutation or MYCN amplification were primarily resistant

The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota

Here, we report on the complete genome sequence of the hyperthermophilic Crenarchaeum Thermoproteus tenax (strain Kra 1, DSM 2078(T)) a type strain of the crenarchaeotal order Thermoproteales. Its circular 1.84-megabase genome harbors no extrachromosomal elements and 2,051 open reading frames are identified, covering 90.6% of the complete sequence, which represents a high coding density. Derived from the gene content, T. tenax is a representative member of the Crenarchaeota. The organism is strictly anaerobic and sulfur-dependent with optimal growth at 86 degrees C and pH 5.6. One particular feature is the great metabolic versatility, which is not accompanied by a distinct increase of genome size or information density as compared to other Crenarchaeota. T. tenax is able to grow chemolithoautotrophically (CO2/H-2) as well as chemoorganoheterotrophically in presence of various organic substrates. All pathways for synthesizing the 20 proteinogenic amino acids are present. In addition, two presumably complete gene sets for NADH:quinone oxidoreductase (complex I) were identified in the genome and there is evidence that either NADH or reduced ferredoxin might serve as electron donor. Beside the typical archaeal A(0)A(1)-ATP synthase, a membrane-bound pyrophosphatase is found, which might contribute to energy conservation. Surprisingly, all genes required for dissimilatory sulfate reduction are present, which is confirmed by growth experiments. Mentionable is furthermore, the presence of two proteins (ParA family ATPase, actin-like protein) that might be involved in cell division in Thermoproteales, where the ESCRT system is absent, and of genes involved in genetic competence (DprA, ComF) that is so far unique within Archaea