13 research outputs found
5, 7-Dihalo-8-quinolinol complex inhibits growth of ovarian cancer cells via the downregulation of expression of Wip1
Purpose: To assess the cytotoxic effect of 5, 7-dihalo-8-quinolinol complex (DHQ) on ovarian cancer cells, and the mechanism of action involved.Methods: DHQ-mediated changes in cell viability were determined using MTT assay, while apoptosis was analyzed with flow cytometry. The effect of DHQ on cell migration was determined using inverted microscopy, while its effect on invasiveness was assessed with Giemsa dyeing. FACS Caliburinstrumentation was employed for analyzing the effect of DHQ on the cell cycle. The protein expressions of Wip1 and P53 were assayed by western blotting.Results: DHQ induced cytotoxicity against A2780 and OVCAR 3 cells in the concentration range of 0.25 - 12 μM (p < 0.05). In A2780 and OVCAR 3 cells, treatment with 12 μMDHQ resulted in 69.34 and 65.46 % apoptosis, respectively. The migratory potential and invasiveness of A2780 and OVCAR3 cells were significantly decreased by 12 μMDHQ, relative to untreated cells (p < 0.05). Moreover, treatment with 12 μMDHQ arrested cell cycle at G1/G0 phase in A2780 and OVCAR3 cells, but downregulated the protein expressions of Wip1 expression in A2780 and OVCAR3 cells.Conclusion: DHQ exerts cytotoxic effect on ovarian cancer cell growth via arrest of cell cycle and activation of apoptosis. Moreover, DHQ inhibits the migratory and invasive abilities of the cells. Thus, DHQ is a potential drug candidate for the management of ovarian cancer.
Keywords: 5,7-Dihalo-8-quinolinol complex, Ovarian cancer, Cytotoxicity, Apoptosis, Invasiveness, Migration, Cell cycl
An Update on Inflamm-Aging: Mechanisms, Prevention, and Treatment
Inflamm-aging is a challenging and promising new branch of aging-related research fields that includes areas such as immunosenescence. Increasing evidence indicates that inflamm-aging is intensively associated with many aging diseases, such as Alzheimer’s disease, atherosclerosis, heart disease, type II diabetes, and cancer. Mounting studies have focused on the role of inflamm-aging in disease progression and many advances have been made in the last decade. However, the underlying mechanisms by which inflamm-aging affects pathological changes and disease development are still unclear. Here, we review studies of inflamm-aging that explore the concept, pathological features, mechanisms, intervention, and the therapeutic strategies of inflamm-aging in disease progression
Preparation and Characterization of Sb2Te3 Thin Films by Coevaporation
Deposition of Sb2Te3 thin films on soda-lime glass substrates by coevaporation of Sb and Te is described in this paper. Sb2Te3 thin films were characterized by x-ray diffraction (XRD), x-ray fluorescence (XRF), atomic force microscopy (AFM), x-ray photoelectron spectroscopy (XPS), electrical conductivity measurements, and Hall measurements. The abnormal electrical transport behavior occurred from in situ electrical conductivity measurements. The results indicate that as-grown Sb2Te3 thin films are amorphous and undergo an amorphous-crystalline transition after annealing, and the posttreatment can effectively promote the formation of Sb-Te bond and prevent oxidation of thin film surface
Multi-mode graphene based terahertz amplitude modulation enhanced by hollow cross H-structured metasurface
Growth behaviors of intermetallic compound layers in Cu/Al joints brazed with Zn–22Al and Zn–22Al–0.05Ce filler metals
Signal enhancement in a lateral flow immunoassay based on dual gold nanoparticle conjugates
Simultaneous preparation of naturally abundant and rare catechins by tannase-mediated biotransformation combining high speed counter current chromatography
Exploring the Underlying Correlation between the Structure and Ionic Conductivity in Halide Spinel Solid-State Electrolytes with Neutron Diffraction
The development of cutting-edge solid-state electrolytes
(SSEs)
entails a deep understanding of the underlying correlation between
the structure and ionic conductivity. Generally, the structure of
SSEs encompasses several interconnected crystal parameters, and their
collective influence on Li+ transport can be challenging
to discern. Here, we systematically investigate the structure–function
relationship of halide spinel LixMgCl2+x (2 ≥ x ≥
1) SSEs. A nonmonotonic trend in the ionic conductivity of LixMgCl2+x SSEs
has been observed, with the maximum value of 8.69 × 10–6 S cm–1 achieved at x = 1.4. The
Rietveld refinement analysis, based on neutron diffraction data, has
revealed that the crystal parameters including cell parameters, Li+ vacancies, Debye–Waller factor, and Li–Cl bond
length assume diverse roles in influencing ionic conductivity of LixMgCl2+x at different
stages within the range of x values. Besides, mechanistic
analysis demonstrates Li+ transport along three-dimensional
pathways, which primarily governs the contribution to ionic conductivity
of LixMgCl2+x SSEs. This study has shed light on the collective influence of crystal
parameters on Li+ transport behaviors, providing valuable
insights into the intricate relationship between the structure and
ionic conductivity of SSEs