Study of interleukin-6 levels in early diagnosis of neonatal sepsis

Background:Neonatal septicemia is one of the commonest causes of neonatal mortality and morbidity. Interleukin-6 Levels appears to be one of the most promising candidate cytokine for early diagnosis of neonatal septicaemia. The aim and objectives of this study was to study the role of IL-6 levels as an early marker for diagnosis of neonatal sepsis and to compare IL-6 levels with other septic markers.  Methods:This is a hospital based prospective study to evaluate the efficacy of IL-6 as an early diagnostic marker of neonatal sepsis. Eighty neonates, delivered in the hospital, having risk factors for neonatal sepsis, along with those coming to hospital with signs and symptoms of sepsis up to 28 days of life (as study group ) along with normal new-borns admitted to the postnatal ward without high risk factors (control group) were enrolled for this study.Results:IL-6 Levels has shown maximum sensitivity of 95.83%, specificity of 87.50%, positive predictive value of 92%, negative predictive value of 93.33% and accuracy of 92.50 %. E. coli was the most common organism responsible for sepsis. CRP was reported to be highly sensitive (84.21%), and  CBC was highly specific (75.00%), IT ratio has sensitivity of 62.5% and specificity of 56.25% while micro-ESR has shown sensitivity of 50.0% and specificity of 62.5%. Out of 80 cases, blood culture (BacTalert) showed growth in 48 cases in study group while two cases in control group. Thus blood culture positivity was 60%.Conclusion:IL-6 test has maximum sensitivity as well as specificity in comparison with other septic markers. Blood culture is the gold standard for the diagnosis of septicemia. CRP is most sensitive while CBC is most specific marker in neonatal sepsis.

Molecular Characterization of Heat Shock Protein 70-1 Gene of Goat (Capra hircus)

Heat shock protein 70 (HSP 70) plays a vital role by bestowing cytoprotection against diverse kinds of stresses. The ubiquitous HSP 70 proteins are the most abundant and temperature sensitive among all the HSPs. The present paper has characterized HSP70-1 cDNA in goat (Capra hircus). Total RNA isolated from goat peripheral blood mononuclear cells was reverse transcribed to cDNA that was used for amplification of HSP 70-1 gene. PCR product (1926 bp) was cloned in pGEM-T easy vector and sequenced. Sequence analysis revealed 1926-bp-long open reading frame of HSP 70-1 gene encoding 641 amino acids in goat, as reported in cattle. At nucleotide level, goat HSP 70-1 was found to be 96–99% similar to that of sheep (partial), cattle, and buffalo whereas the similarity at amino acid level was 95–100%. Nonsynonymous substitutions exceeding synonymous substitutions indicate the evolution of this protein through positive selection among domestic animals. Goat and sheep appear to have diverged from a common ancestor in phylogenetic analysis. Predicted protein structures of goat HSP 70 protein obtained from deduced amino acid sequence indicated that the functional amino acids involved in chaperoning through ATPase hydrolytic cycle and in uncoating of clathrin coated vesicles are highly conserved

Correlation of Serum Insulin-Like Growth Factor-1 and Hand Wrist Radiographs as Skeletal Maturity Indicators: An ex-vivo Study

Introduction: Conventional Hand and Wrist radiographs, have certain drawbacks of exposing orthodontic patients to unnecessary radiation hazards and subjectivity to errors. Serum insulin-like growth factor 1 (IGF-1) levels have been reported to increase until the pubertal peak in literature. The present study aims to assess the relationship between serum IGF-1 and skeletal maturity indicators Materials and Methods: Lateral cephalograms of 60 patients were assigned SMI stage according to Fishman’s classification system. The serum IGF-1 levels of the patients were also evaluated. Correlation between the serum IGF-1 levels, age of the patient, and their SMI stage was analyzed. Results: Pearson's coefficient of correlation revealed a non-significant weak positive correlation (p=0.69; >0.05) between age and IGF-1 levels, and a non-significant weak negative correlation (p =0.52; >0.05) between SMI stages and IGF-1 levels. Results of the ANOVA test indicated that there was no significant difference between mean IGF-1 levels across the different age groups and eleven SMI groups. However, there was a significant difference noted in the mean IGF-1 levels and the restructured SMI categories and groups. Conclusion: The moderate correlation between age and serum IGF-1 fluctuations during puberty underscores the hormone's pivotal role in adolescent growth. This positions serum IGF-1 as a potentially specific and reliable marker for assessing mandibular growth modifications, offering a radiation-free alternative to conventional radiographic methods

Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin–induced intestinal fluid secretion

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Bridge Monitoring System Using IOT

In countries like India there is powerful focus on national infrastructure. New bridges are built each year and the maintenance of those bridges is frequently ignored. The present structures uses very complex and excessive fee wired network and it additionally required high upkeep for optical fiber machine. So the primary objective of this task is to build a cheap bridge tracking machine for developing international locations like India. This project aim to simplify the system for selecting bridge tracking devices. Many bridges within the India are obsolete or structurally deficient to safely increase the life of those bridges, inspection would be vital. Bridge engineers have many duties and it's far not possible to expect one to know. Our device will sense the crack inside the bridge and signal might be given to govern room immediately to stop cars

Assessment of Population-Based HIV RNA Levels in a Rural East African Setting Using a Fingerprick-Based Blood Collection Method

Background. Population-based human immunodeficiency virus type 1 (HIV-1) RNA levels (viral load [VL]) are proposed metrics for antiretroviral therapy (ART) program effectiveness. We estimated population-based HIV RNA levels using a fingerprick-based approach in a rural Ugandan community implementing rapid ART scale-up

Application of Magnetic Nanoparticles to Gene Delivery

Nanoparticle technology is being incorporated into many areas of molecular science and biomedicine. Because nanoparticles are small enough to enter almost all areas of the body, including the circulatory system and cells, they have been and continue to be exploited for basic biomedical research as well as clinical diagnostic and therapeutic applications. For example, nanoparticles hold great promise for enabling gene therapy to reach its full potential by facilitating targeted delivery of DNA into tissues and cells. Substantial progress has been made in binding DNA to nanoparticles and controlling the behavior of these complexes. In this article, we review research on binding DNAs to nanoparticles as well as our latest study on non-viral gene delivery using polyethylenimine-coated magnetic nanoparticles

Therapy related myelodysplastic syndrome: A case report and review of literature

Therapy related myeloid neoplasm is directly related to previous cytotoxic chemotherapy or radiation therapy. We present a 47-year-old lady who developed therapy related myelodysplastic syndrome (MDS) 2.5 years after she received four cycles of chemotherapy and local radiation therapy for carcinoma breast. She presented with bicytopenia with trilineage dyspoiesis in the peripheral blood, bone marrow aspirate and biopsy. Fluorescent in-situ hybridization studies did not reveal any of the common abnormalities associated with MDS. A diagnosis of therapy related MDS was rendered. Different studies have shown that patients treated with alkylating agents and ionizing radiation present as MDS with a latent period of 3-10 years. Our patient developed MDS within 2.5 years of starting chemotherapy and radiotherapy and did not reveal any of the conventional cytogenetic abnormalities. It highlights the importance of simple tests like a complete blood count and peripheral blood smear examination in follow-up of the patients treated with chemotherapy

Review on Design and Analysis of SS-PP Honeycomb Master leaf of Mahindra Bolero Pickup

In order to economize energy and conserve naturalresources, weight reduction has been the main focus of automobile manufacturers in the present scenario. The increasing competition and innovations in automobile sector tends to modify the existing products or replacing old products by new and advanced materials. Composite sandwich construction has been recognized as a promising concept for structural design of light weight transportation systems such as aircraft, high-speed trains and fast ships. A sandwich development gives great basic effectiveness, i.e. with high proportion of quality to weight. Regardless of the fact that the idea of sandwich development is not new, it has been fundamentally embraced for non-quality piece of structures in the most recent decade. The aim of the present study is to investigate the strength characteristics of honeycomb sandwich panels with core of polypropylene theoretically and experimentally. FEA of the both existing and honeycomb master leaf will be carry out. A series of strength tests will carry out on composite honeycomb-cored sandwich panel specimen in three point bending test. Simplified theories are applied to analyse bending deformation, buckling or ultimate strength. The structural failure characteristics of composite sandwich panels are discussed

Synthesis of coumarin-3-carboxylic acids in waste curd water: A green approach

An efficient and green protocol has been developed for the synthesis of derivatives of coumarin-3-carboxylic acid using waste curd water as a catalytic solvent. Curd water successfully catalyzes the reaction of 2-hydroxybenzaldehydes with dimethyl malonate under ultrasonic irradiation (40 °C) to construct different scaffolds of coumarin-3-carboxylic acid, with good to outstanding yields. The use of biodegradable solvents, sustainability, low reaction duration, mild reaction conditions without metals and Lewis acids, excellent yields, and compatibility with a wide range of electronically diverse substrates are all advantages of this synthesis process. Acidic curd water, which acts as a biological catalyst as well as a solvent for the reaction under ultrasonic irradiation, may be a better green alternative to some standard methods for synthesizing coumarin-3-carboxylic acids