20 research outputs found

    Processing Oscillatory Data with PDV

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    Author Institution: Lawrence Livermore National LaboratorySlides presented at the 2nd Annual Photonic Doppler Velocimetry (PDV) Workshop held at Lawrence Livermore National Laboratory, Livermore, California, August 16-17, 2007

    Long-term variability of proglacial groundwater-fed hydrological systems in an area of glacier retreat, Skeioararsandur, Iceland

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    Proglacial groundwater‐fed features, such as seeps, substantially impact proglacial geomorphology, hydrology, and ecology. However, there is a paucity of research on the impacts of climate change and glacier retreat on the extent of these important features. This paper aims to investigate the impact of glacier retreat on proglacial groundwater levels and on the extent of groundwater‐fed seeps. Research has taken place in western Skeiðarársandur, the large proglacial outwash plain of Skeiðarárjökull, a retreating temperate glacier in southeast Iceland. Changes in the extent of proglacial groundwater seeps were mapped using historical aerial photographs from 1986, 1997, and 2012. Proglacial groundwater levels were monitored in shallow boreholes between 2000 and 2012. The western margin of Skeiðarárjökull has retreated approximately 1 km beyond its position in 1986. However, this retreat was punctuated by short periods of readvance. The geomorphology and groundwater systems at the site were substantially impacted by the November 1996 jökulhlaup, whose deposits altered approximately 18% of the area of groundwater seeps. The surface areas of groundwater seeps and lakes in the study area have declined by ~97% between 1986 and 2012. Most of the decline took place after 1997, when the mean annual rate of retreat increased three‐fold. Groundwater levels also declined substantially between 2000 and 2012, although this trend varies spatially. The paper provides a conceptual model of the controls on proglacial shallow groundwater systems. Direct impacts of glacier retreat are suggested as the main cause for the declines in proglacial groundwater levels and in the extent of groundwater seeps. These declines are expected to adversely impact sandur ecology

    High-Throughput High-Resolution Class I HLA Genotyping in East Africa

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    HLA, the most genetically diverse loci in the human genome, play a crucial role in host-pathogen interaction by mediating innate and adaptive cellular immune responses. A vast number of infectious diseases affect East Africa, including HIV/AIDS, malaria, and tuberculosis, but the HLA genetic diversity in this region remains incompletely described. This is a major obstacle for the design and evaluation of preventive vaccines. Available HLA typing techniques, that provide the 4-digit level resolution needed to interpret immune responses, lack sufficient throughput for large immunoepidemiological studies. Here we present a novel HLA typing assay bridging the gap between high resolution and high throughput. The assay is based on real-time PCR using sequence-specific primers (SSP) and can genotype carriers of the 49 most common East African class I HLA-A, -B, and -C alleles, at the 4-digit level. Using a validation panel of 175 samples from Kampala, Uganda, previously defined by sequence-based typing, the new assay performed with 100% sensitivity and specificity. The assay was also implemented to define the HLA genetic complexity of a previously uncharacterized Tanzanian population, demonstrating its inclusion in the major East African genetic cluster. The availability of genotyping tools with this capacity will be extremely useful in the identification of correlates of immune protection and the evaluation of candidate vaccine efficacy
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