Testing the Underlying Chemical Principles of the Biotic Ligand Model (BLM) to Marine Copper Systems: Measuring Copper Speciation Using Fluorescence Quenching

Speciation of copper in marine systems strongly influences the ability of copper to cause toxicity. Natural organic matter (NOM) contains many binding sites which provides a protective effect on copper toxicity. The purpose of this study was to characterize copper binding with NOM using fluorescence quenching techniques. Fluorescence quenching of NOM with copper was performed on nine sea water samples. The resulting stability con- stants and binding capacities were consistent with literature values of marine NOM, show- ing strong binding with log K values from 7.64 to 10.2 and binding capacities ranging from 15 to 3110 nmole mg C −1 . Free copper concentrations estimated at total dissolved copper concentrations corresponding to previously published rotifer effect concentrations, in the same nine samples, were statistically the same as the range of free copper calculated for the effect concentration in NOM-free artificial seawater. These data confirms the applicability of fluorescence spectroscopy techniques for NOM and copper speciation characterization in sea water and demonstrates that such measured speciation is consistent with the chemical principles underlying the Biotic Ligand Model (BLM) approach for bioavailability-based metals risk assessment

Digging the Rabbit Hole, COVID-19 Edition: Anti-Vaccine Themes and the Discourse Around COVID-19

This article draws on a broadcast popular among the anti-vaccine community to map out six themes used by the broadcast to mislead viewers about COVID-19. The themes are the claim that “they” e government and pharma e are lying to you, claims that COVID-19 is an excuse to remove civil liberties, viewing everyone as an expert, claiming that science cannot save us, skewing the science, and a claim that “they” are out to harm the viewers. The article points out that similar themes are used to mislead followers with anti-vaccine information. It highlights the concern that these themes will not only mislead people who are already anti-vaccine about the pandemic, but may draw in people who are not anti-vaccine but are seeking information about COVID-19, and suggests some options for dealing with the misinformation. Scientists benefit from understanding these claims, as we are often tasked with ttals to this misinformation

COVID-19 Vaccine Acceptance: We Need to Start Now

In this perspective, we discuss the importance of developing a vaccine to help curb transmission of severe acute respiratory syndrome coronavirus 2. The question remains: Once a safe and effective vaccine is developed, will the public be willing to get it? We present information from one of the first tracking polls to assess public attitudes and perceptions toward a possible coronavirus disease 2019 vaccine that suggests public hesitancy over a potential vaccine, concern regarding accelerating clinical trials, and unease over the vaccine approval process. Public health experts, government officials, advocates, and others in the scientific community should respect the signals of hesitancy and communicate sensitivity, applying lessons not only to how we message, but also in how we build this urgently needed vaccine if we are to have successful uptake once available

Advancing Science through Conversations: Bridging the Gap between Blogs and the Academy

Blogs have stormed the Internet, providing an interactive medium for rapid and wide-reaching information dispersal. But is there a place for blogs in academia

The Evolution of the CareerAdvance® Program in Tulsa, Oklahoma

CareerAdvance®n collaboration with a multi-disciplinary team of partners, the Ray Marshall Center (RMC) is developing and implementing a sectoral workforce development strategy for low-skilled, low-income parents of children served by early childhood programs in Tulsa, Oklahoma. There is emerging evidence that children whose parents hold stable jobs with progressively rising incomes exhibit better academic and behavioral outcomes. RMC and its partners have undertaken a dual-generation approach to poverty reduction that strengthens the investment in early childhood development by equipping Head Start parents with workforce training and gainful employment opportunities. This approach employs a more holistic model than traditional workforce development programs, as it also includes employee counseling and other support services to help parents complete training and adult basic education, retain their jobs, advance in their careers, and become economically self-sufficient. The goal is to develop a sustainable sectoral strategy that can be replicated beyond Tulsa to other communities across the nation. In the first phase of the project (2008-2009), RMC designed a sectoral job development strategy focused on industries featuring jobs that pay well and provide much-needed employee benefits (e.g., health insurance, annual and sick leave) as well as career advancement opportunities. In April 2009, Community Action Program of Tulsa County launched the pilot, CareerAdvance, at two Head Start sites in Tulsa involving 15 parents. The components of the CareerAdvance are 1) GED and college readiness instruction, as needed; 2) skills training in the healthcare sector progressing from Certified Nursing Aide to Licensed Practical Nurse to Registered Nurse; 3) weekly peer support meetings addressing a flexible set of topics (e.g., life skills, work readiness, family finances); 4) conditional cash incentives (up to $3,000 a year) for participants meeting specified benchmarks to reinforce continued participation and help offset foregone earnings; and 5) workforce intermediation between healthcare employers and training institutions provided through Workforce Tulsa. The report on the project’s first year of operation is available at the link below. In partnership with Harvard University and the University of Oklahoma – Tulsa School of Medicine, a second pilot site was opened in July 2009 at a Tulsa Educare Center. The second pilot, EduCareers, includes all components described above as well as enhanced mental health services for participating households, curriculum enhancements for the children, parent engagement training, and a medical home. The CareerAdvance project has now been expanded to 2015 with support from the U.S. Department of Health and Human Services’ Administration for Children and Families. RMC and partners at Northwestern and Columbia University have been engaged to provide ongoing on data collection, implementation and outcomes analysis of project participants.George Kaiser Family Foundation, W. K. Kellogg Foundation, U.S. Department of Health and Human Services' Administration for Children and FamiliesRay Marshall Center for the Study of Human Resource

SARS-CoV-2 transmission dynamics should inform policy

Funding: JM is supported in part by the U.S. National Institute of Allergy and Infectious Diseases [K01AI122853].It is generally agreed that striking a balance between resuming economic and social activities and keeping the effective reproductive number (R0) below 1 using non-pharmaceutical interventions is an important goal until and even after effective vaccines become available. Therefore, the need remains to understand how the virus is transmitted in order to identify high-risk environments and activities that disproportionately contribute to its spread so that effective preventative measures could be put in place. Contact tracing and household studies in particular provide robust evidence about the parameters of transmission. In this viewpoint, we discuss the available evidence from large-scale, well-conducted contact tracing studies from across the world and argue that SARS-CoV-2 transmission dynamics should inform policy decisions about mitigation strategies for targeted interventions according to the needs of the society by directing attention to the settings, activities and socioeconomic factors associated with the highest risks of transmission.PostprintPeer reviewe

Generation and characterization of monoclonal antibodies that recognize human and murine supervillin protein isoforms

Supervillin isoforms have been implicated in cell proliferation, actin filament-based motile processes, vesicle trafficking, and signal transduction. However, an understanding of the roles of these proteins in cancer metastasis and physiological processes has been limited by the difficulty of obtaining specific antibodies against these highly conserved membrane-associated proteins. To facilitate research into the biological functions of supervillin, monoclonal antibodies were generated against the bacterially expressed human supervillin N-terminus. Two chimeric monoclonal antibodies with rabbit Fc domains (clones 1E2/CPTC-SVIL-1; 4A8/CPTC-SVIL-2) and two mouse monoclonal antibodies (clones 5A8/CPTC-SVIL-3; 5G3/CPTC-SVIL-4) were characterized with respect to their binding sites, affinities, and for efficacy in immunoblotting, immunoprecipitation, immunofluorescence microscopy and immunohistochemical staining. Two antibodies (1E2, 5G3) recognize a sequence found only in primate supervillins, whereas the other two antibodies (4A8, 5A8) are specific for a more broadly conserved conformational epitope(s). All antibodies function in immunoblotting, immunoprecipitation and in immunofluorescence microscopy under the fixation conditions identified here. We also show that the 5A8 antibody works on immunohistological sections. These antibodies should provide useful tools for the study of mammalian supervillins

Protein futures for Western Europe: potential land use and climate impacts in 2050

Multiple production and demand side measures are needed to improve food system sustainability. This study quantified the theoretical minimum agricultural land requirements to supply Western Europe with food in 2050 from its own land base, together with GHG emissions arising. Assuming that crop yield gaps in agriculture are closed, livestock production efficiencies increased and waste at all stages reduced, a range of food consumption scenarios were modelled each based on different ‘protein futures’. The scenarios were as follows: intensive and efficient livestock production using today’s species mix; intensive efficient poultry–dairy production; intensive efficient aquaculture–dairy; artificial meat and dairy; livestock on ‘ecological leftovers’ (livestock reared only on land unsuited to cropping, agricultural residues and food waste, with consumption capped at that level of availability); and a ‘plant-based eating’ scenario. For each scenario, ‘projected diet’ and ‘healthy diet’ variants were modelled. Finally, we quantified the theoretical maximum carbon sequestration potential from afforestation of spared agricultural land. Results indicate that land use could be cut by 14–86 % and GHG emissions reduced by up to approximately 90 %. The yearly carbon storage potential arising from spared agricultural land ranged from 90 to 700 Mt CO2 in 2050. The artificial meat and plant-based scenarios achieved the greatest land use and GHG reductions and the greatest carbon sequestration potential. The ‘ecological leftover’ scenario required the least cropland as compared with the other meat-containing scenarios, but all available pasture was used, and GHG emissions were higher if meat consumption was not capped at healthy levels.&nbsp