The Concept of “IT Artifact” Has Outlived Its Usefulness and Should Be Retired Now

Vastly inconsistent definitions of the term “the IT artifact” in leading journals and conferences demonstrate why it no longer means anything in particular and should be retired from the active IS lexicon. Examples from the literature show why artifact-cousins, such as the IS artifact, sociotechnical artifact, social artifact, and ensemble artifact should be used with great care, if not retired as well. Any void created by these retirements could be filled through the following approaches: 1) relabeling with simple terms that are immediately understandable, 2) adopting guidelines for making sense of the whole X-artifact family, and 3) sidestepping the IT artifact and focusing directly on IT-enabled work systems in organizations

Playing projects: identifying flow in the 'lived experience'

The purpose of this paper is to contribute to the 'lived experience' of projects discourse. The research study uses an arts-based research method (musical improvisation on a xylophone and/or glockenspiel) to access the participant's perception of their experience of managing a project. Participants are then asked to explain their improvisation and therefore their experience. Key findings were that participants described their 'lived experience' of project managing as having 'ups and downs', including challenges and issues, and as experiencing variations in emotions over the project lifecycle. Csikszentmihalyi's flow theory is used to show that these 'lived experience' findings support a Heideggerian paradigm and personal perspective of what a project is. Projectness is not a characteristic of the activity itself. A project is a personal phenomenon defined in terms of the relationship between the individual or organisation and activity. It is dependent on capability versus the challenge presented by the activity

Multi-person Pose Estimation in Soccer Videos with Convolutional Neural Networks

Pose estimation is the problem of detecting poses of people in images, multiperson pose estimation is the problem of detecting poses of multiple persons in images. This thesis investigates multi-person pose estimation by applying the associative embedding method on images from soccer videos. Three models are compared, first a pre-trained model, second a fine-tuned model and third a model extended to handle image sequences. The pre-trained method performed well on soccer images and the fine-tuned model performed better then the pre-trained model. The image sequence model performed equally as the fine-tuned model but not better. This thesis concludes that the associative embedding model is a feasible option for pose estimation in soccer videos and should be further researched

Peptide-Liposome Model Systems for Triggered Release

Liposomes are widely used in drug delivery to improve drug efficacy and to reduce side effects. For liposome-encapsulated drugs to become bioavailable and provide a therapeutic effect they must be released, which typically is a slow process that primarily relies on passive diffusion, liposome rupture or endocytotic uptake. Achieving drug concentrations within the therapeutic window can thus be challenging, resulting in poor efficacy and higher risks drug resistance. Finding means to modulate lipid membrane integrity and to trigger rapid and efficient release of liposomal cargo is thus critical to improve current and future liposomal drug delivery systems. The possibilities to tailor lipid composition and surface functionalization is vital for drug delivery applications but also make liposomes attractive model systems for studies of membrane active biomolecules. The overall aim of this thesis work has been to develop new strategies for triggering and controlling changes in lipid membrane integrity and to study the interactions of membrane active peptides with model lipid membranes using both de novo designed and biologically derived synthetic amphipathic cationic peptides. Two different sets of designed peptides have been explored that can fold and heterodimerize into a coiled coil and helix-loop-helix fourhelix bundle, respectively. Conjugation of the cationic lysine rich peptides to liposomes triggered a rapid and concentration dependent release. The additions of their corresponding glutamic acid-rich complementary peptides inhibited the release of liposomal cargo. Possibilities to reduce the inhibitory effect by both proteolytic digestion of the inhibitory peptide and by means of heterodimer exchange have been investigated. Moreover, the effects of peptide size and composition and ability to fold have been studied in order to elucidate the factors that influence the membrane permeabilizing effects of the peptides. In addition, the membrane activity of a the two-peptide bacteriocin PLNC8α and PLNC8β has been explored using liposomes as a model system. PLNC8αβ are expressed by Lactobacillus plantarum and were shown to display pronounced membrane-partition folding coupling, leading to rapid release of liposome encapsulated carboxyfluorescein. PLNC8αβ also kill and suppressed growth of the gram-negative bacteria Porphyromonas gingivalis by efficiently damaging the bacterial membrane. Although membrane active peptides are highly efficient in perturbing lipid membrane integrity, possibilities to trigger release using external stimuli are also of large interest for therapeutic applications. Light-induced heating of liposome encapsulated gold nanoparticles (AuNPs) has been shown by others as a potential strategy to trigger drug release. To facilitate fabrication of thermoplasmonic liposome systems we developed a simple method for synthesis of small AuNPs inside liposomes, using the liposomes as nanoscale reaction vessels. The work presented in this thesis provides new knowledge and techniques for future development of liposome-based drug delivery systems, peptide-based therapeutics and increase our understanding of peptide-lipid interactions