Antihyperlipidemic Activity of Stellaria Media Leaves on Induced Hyperlipidemic Sprague Dawley Rats

Plant materials are used throughout the developed and developing world as home remedies, in over-the-counter drug products, and as raw material for the pharmaceutical industry, and they represent a substantial proportion of the global drug market. Certain herbs have become popular over the years, but the public, medical practitioners and the media still have a poor understanding of herbal medicine. Evidence is emerging on the dangers of herbs. As in most situations, the truth lies hidden under the media hype, poorly understood science, and exaggerated claims. Lack of experience, information, and education about herbs make consumers, physicians, and other orthodox health care provider’s easy victims of market exploitation and herbal myths. There is no rational reason behind the tendency to equate “natural” with “harmlessness.” The fact that something is natural does not necessarily make it safe or effective. In addition, a lack of knowledge of phytochemistry leads to misinterpretation and misunderstanding. It is very likely that some herbs will have side effects, interact with other medications, and be toxic. Information on isolated constituents should not be applied directly to the whole herb and studies on in vitro forms should not be confused with oral administration which was established by pharmacological screenings. In current scenario, herbs are the potent sources of medicines used in the treatment of various disease and disorders. Since, plants are used as medicine there is prompt need of evaluation of plant species, therefore, the present work was conceived to evaluate the phytochemical and pharmacological screening of few Indian medicinal plants. Thus the results of the present investigation clearly indicated that the selected medicinal plants possess good antihyperlipidemic activity in atherogenic diet induced hyperlipidemic rats and led to the development of new Herbal formulation possessing antihyperlipidemic and antiatherosclerotic activities Hence it is going to be concluded that the potential benefits of the extracts of Stellaria media has been demonstrated well in advance and can be used further to demonstrate the antihyperlipidemic as well as controlling of both triglyceride levels and reducing the risk of factors of cholesterol inducers. The aforementioned results of the research suggest that the Stellaria media, found to have the potential antihyperlipidemic action. The results found are encouraging for further studies on the selected plants and to identify the bioactive compounds

Structure based virtual screening of novel inhibitors against multidrug resistant superbugs

Pathogenic microorganisms are persistently expressing resistance towards present generation antibiotics and are on the verge of joining the superbug family. Recent studies revealed that, notorious pathogens such as Salmonella typhi, Shigella dysenteriae and Vibrio cholerae have acquired multiple drug resistance and the treatment became a serious concern. This necessitates an alternative therapeutic solution. Present study investigates the utility of computer aided method to study the mechanism of receptor-ligand interactions and thereby inhibition of virulence factors (shiga toxin of Shigella dysenteriae, cholera toxin of Vibrio cholerae and hemolysin-E of Salmonella typhi) by novel phytoligands. The rational designs of improved therapeutics require the crystal structure for the drug targets. The structures of the virulent toxins were identified as probable drug targets. However, out of the three virulent factors, the structure for hemolysin-E is not yet available in its native form. Thus, we tried to model the structure by homology modeling using Modeller 9v9. After extensive literature survey, we selected 50 phytoligands based on their medicinal significance and drug likenesses. The receptor-ligands interactions between selected leads and toxins were studied by molecular docking using Auto Dock 4.0. We have identified two novel sesquiterpenes, Cadinane [(1S, 4S, 4aS, 6S, 8aS)- 4- Isopropyl- 1, 6- dimethyldecahydronaphthalene] and Cedrol [(8α)-Cedran-8-ol] against Shiga (binding energy -5.56 kcal/mol) and cholera toxins (binding energy -5.33 kcal/mol) respectively which have good inhibitory properties. Similarly, a natural Xanthophyll, Violaxanthin [3S, 3'S, 5R, 5'R, 6S, 6'S)-5, 5', 6, 6'-Tetrahydro-5, 6:5', 6'-diepoxy-β, β-carotene-3, 3'-diol] was identified as novel therapeutic lead for hemolysin-E (binding energy of –5.99 kcal/mol). This data provide an insight for populating the pool of novel inhibitors against various drug targets of superbugs when all current generation drugs seem to have failed

Recent Aspects on the Pathogenesis Mechanism, Animal Models and Novel Therapeutic Interventions for Middle East Respiratory Syndrome Coronavirus Infections

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is an emerging zoonotic virus considered as one of the major public threat with a total number of 2 298 laboratory-confirmed cases and 811 associated deaths reported by World Health Organization as of January 2019. The transmission of the virus was expected to be from the camels found in Middle Eastern countries via the animal and human interaction. The genome structure provided information about the pathogenicity and associated virulent factors present in the virus. Recent studies suggested that there were limited insight available on the development of novel therapeutic strategies to induce immunity against the virus. The severities of MERS-CoV infection highlight the necessity of effective approaches for the development of various therapeutic remedies. Thus, the present review comprehensively and critically illustrates the recent aspects on the epidemiology of the virus, the structural and functional features of the viral genome, viral entry and transmission, major mechanisms of pathogenesis and associated virulent factors, current animal models, detection methods and novel strategies for the development of vaccines against MERS-CoV. The review further illustrates the molecular and computational virtual screening platforms which provide insights for the identification of putative drug targets and novel lead molecules toward the development of therapeutic remedies

Recent Perspectives on Genome, Transmission, Clinical Manifestation, Diagnosis, Therapeutic Strategies, Vaccine Developments, and Challenges of Zika Virus Research

One of the potential threats to public health microbiology in 21st century is the increased mortality rate caused by Zika virus (ZIKV), a mosquito-borne flavivirus. The severity of ZIKV infection urged World Health Organization (WHO) to declare this virus as a global concern. The limited knowledge on the structure, virulent factors, and replication mechanism of the virus posed as hindrance for vaccine development. Several vector and non-vector-borne mode of transmission are observed for spreading the disease. The similarities of the virus with other flaviviruses such as dengue and West Nile virus are worrisome; hence, there is high scope to undertake ZIKV research that probably provide insight for novel therapeutic intervention. Thus, this review focuses on the recent aspect of ZIKV research which includes the outbreak, genome structure, multiplication and propagation of the virus, current animal models, clinical manifestations, available treatment options (probable vaccines and therapeutics), and the recent advancements in computational drug discovery pipelines, challenges and limitation to undertake ZIKV research. The review suggests that the infection due to ZIKV became one of the universal concerns and an interdisciplinary environment of in vitro cellular assays, genomics, proteomics, and computational biology approaches probably contribute insights for screening of novel molecular targets for drug design. The review tried to provide cutting edge knowledge in ZIKV research with future insights required for the development of novel therapeutic remedies to curtail ZIKV infection

Screening of potential lead molecules against prioritised targets of multi-drug-resistant-<i>Acinetobacter baumannii</i> – insights from molecular docking, molecular dynamic simulations and <i>in vitro</i> assays

<p><i>Acinetobacter baumannii</i>, an opportunistic pathogen, has become multi-drug resistant (MDR) to major classes of antibacterial and poses grave threat to public health. The current study focused to screen novel phytotherapeutics against prioritised targets of <i>Acinetobacter baumannii</i> by computational investigation. Fourteen potential drug targets were screened based on their functional role in various biosynthetic pathways and the 3D structures of 9 targets were retrieved from Protein Data Bank and others were computationally predicted. By extensive literature survey, 104 molecules from 48 herbal sources were screened and subjected to virtual screening. Ten clinical isolates of <i>A. baumannii</i> were tested for antibiotic susceptibility towards clinafloxacin, imipenem and polymyxin-E. Computational screening suggested that Ajmalicine ((19α)-16, 17-didehydro-19-methyloxayohimban-16-carboxylic acid methyl ester from <i>Rauwolfia serpentina</i>), Strictamin (Akuammilan-17-oic acid methyl ester from <i>Alstonia scholaris</i>) and Limonin (7, 16-dioxo-7, 16-dideoxylimondiol from <i>Citrus sps</i>) exhibited promising binding towards multiple drug targets of <i>A. baumannii</i> in comparison with the binding between standard drugs and their targets. Limonin displayed promising binding potential (binding energy −9.8 kcal/mol) towards diaminopimelate epimerase (DapF) and UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). Ajmalicine and Strictamin demonstrated good binding potential (−9.5, −8.5 kcal/mol, respectively) towards MurA and shikimate dehydrogenase (−7.8 kcal/mol). Molecular dynamic simulations further validated the docking results. <i>In vitro</i> assay suggested that the tested isolates exhibited resistance to clinafloxacin, imipenem and polymyxin-E and the herbal preparations (crude extract) demonstrated a significant antibacterial potential (<i>p</i> ≤ .05). The study suggests that the aforementioned lead candidates and targets can be used for structure-based drug screening towards MDR <i>A. baumannii</i>.</p

Nanoparticle Fullerene (C60) demonstrated stable binding with antibacterial potential towards probable targets of drug resistant <i>Salmonella typhi</i> – a computational perspective and <i>in vitro</i> investigation

<p><i>Salmonella typhi</i>, a Gram negative bacterium, has become multidrug resistant (MDR) to wide classes of antibacterials which necessitate an alarming precaution. This study focuses on the binding potential and therapeutic insight of Nano-Fullerene C60 towards virulent targets of <i>Salmonella typhi</i> by computational prediction and preliminary <i>in vitro</i> assays. The clinical isolates of <i>Salmonella typhi</i> were collected and antibiotic susceptibility profiles were assessed. The drug targets of pathogen were selected by rigorous literature survey and gene network analysis by various metabolic network resources. Based on this study, 20 targets were screened and the 3D structures of few drug targets were retrieved from PDB and others were computationally predicted. The structures of nanoleads such as Fullerene C60, ZnO and CuO were retrieved from drug databases. The binding potential of these nanoleads towards all selected targets were predicted by molecular docking. The best docked conformations were screened and concept was investigated by preliminary bioassays. This study revealed that most of the isolates of <i>Salmonella typhi</i> were found to be MDR (<i>p</i> < .05). The theoretical models of selected drug targets showed high stereochemical validity. The molecular docking studies suggested that Fullerene C60 showed better binding affinity towards the drug targets when compared to ZnO and CuO. The preliminary <i>in vitro</i> assays suggested that 100 μg/L Fullerene C60 posses significant inhibitory activities and absence of drug resistance to this nanoparticle. This study suggests that Fullerene C60 can be scaled up as probable lead molecules against the major drug targets of MDR <i>Salmonella typhi</i>.</p