57 research outputs found

    Homography-based ground plane detection using a single on-board camera

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    This study presents a robust method for ground plane detection in vision-based systems with a non-stationary camera. The proposed method is based on the reliable estimation of the homography between ground planes in successive images. This homography is computed using a feature matching approach, which in contrast to classical approaches to on-board motion estimation does not require explicit ego-motion calculation. As opposed to it, a novel homography calculation method based on a linear estimation framework is presented. This framework provides predictions of the ground plane transformation matrix that are dynamically updated with new measurements. The method is specially suited for challenging environments, in particular traffic scenarios, in which the information is scarce and the homography computed from the images is usually inaccurate or erroneous. The proposed estimation framework is able to remove erroneous measurements and to correct those that are inaccurate, hence producing a reliable homography estimate at each instant. It is based on the evaluation of the difference between the predicted and the observed transformations, measured according to the spectral norm of the associated matrix of differences. Moreover, an example is provided on how to use the information extracted from ground plane estimation to achieve object detection and tracking. The method has been successfully demonstrated for the detection of moving vehicles in traffic environments

    12-Pulse LCI synchronous drive for a 20 MW compressor : modeling, simulation and measurements

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    Abstract – The current source thyristor inverter is still today a suitable choice for supplying high power adjustable speed drives for pumps or compressors. This paper presents a 12-pulse LCI (load commutated inverter) synchronous drive for a 20 MW compressor, it focuses on the following main aspects: modeling of the complete drive system, numerical simulation and comparison between calculated and measured values of the pulsating torque components in steady-state operations. The modeling of the six- phase synchronous motor is obtained by using a new virtual test- platform for large electrical machines. The pulsating torque components are measured in real time with a digital transient torque measurement device

    On the Identification of the Synchronous Machine Parameters Using Standstill DC Decay Test.

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    This paper presents a refined approach to obtain the parameters of synchronous machine equivalent circuits from standstill DC decay tests. A dedicated program for the time- constants and reactances identification was developed and applied for both d- and q- axis, as well as for a random position of the rotor, with good results

    TLSim: A software for Electrical Public Transportation Systems

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    The Laboratory of Electrical Machines of the Swiss Federal Institute of Technology (Lausanne) has developed a software (TLSim) for the simulation of electrical urban transportation systems. This program can simulate the behaviour of the power supply network (current and voltage level at any point of the topology) comprising uni- and bi- directionnal sub-stations. In the network the vehicules (trolleybus) may run along any defined path through different electrical supply sectors. Several models of trolleybus are available, one of these allowing for the injection of current back in the supply network. The operating schedule of the buses as well as the geographical data of the network are provided by the user. This program is a perfect tool for system planning as ascertained by its use by a Swiss transportation operator. A comparison of simulation and measurements on a real network has been performed with satisfactory results. This software evolved from a specific study to a flexible program still being extended with new developments. The latest of such developments being the study of the influence of capacitors in helping the system to keep voltages over a specified level mainly by supplying the surge current during the acceleration of the trolleybuses

    Mechanical properties of porous asphalt, recommendations for standardization

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    Porous Asphalt (PA) is used worldwide for its favorable splash and spray properties and its reduction of aquaplaning under rainy conditions as well as its noise reduction properties. Switzerland started using PA in 1979 with mixed results. According to a survey taken in 2004, nine of the 26 cantons use PA. In particular, canton Vaud in western Switzerland is known as one of the leaders in promoting and using PA. Currently, 1/3 of the Vaud motorways are covered with porous asphalt and the use of PA is planned to be extended to most of the motorway surfaces in the canton Vaud up to an altitude of 600m. In addition, there are several bridge trial sections with PA. After the initial survey of the literature appropriate mechanical tests for porous asphalt were chosen (Table 8. 1). At the same time a survey of current experience with porous asphalt in Switzerland was conducted (Appendix 2). Tests were performed on laboratory prepared specimens (AG1, AG4, AG5, VD7, VD8, VD9) and cores (VD2, VD3, VD4, VD5, VS6, AG2, AG3, VD10) taken from selected pavements chosen based on the feedback from various cantons. The behavior of the selected materials was also assessed using an analytical model. Laboratory tests allowed the comparison of core performance with that of laboratory prepared specimen as well as comparison with field performance. Based on the results two mixes were optimized (VD9, AG5) and recommendations for mechanical tests appropriate for porous asphalt were made

    Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

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    The aim of this study was to compare different cell sources and culture conditions to obtain endothelial progenitor cells (EPCs) with predictable antigen pattern, proliferation potential and in vitro vasculogenesis. Pig mononuclear cells were isolated from blood (PBMCs) and bone marrow (BMMCs). Mesenchymal stem cells (MSCs) were also derived from pig bone marrow. Cells were cultured on fibronectin in the presence of a high concentration of VEGF and low IGF-1 and FGF-2 levels, or on gelatin with a lower amount of VEGF and higher IGF-1 and FGF-2 concentrations. Endothelial commitment was relieved in almost all PBMCs and BMMCs irrespective of the protocol used, whilst MSCs did not express a reliable pattern of EPC markers under these conditions. BMMCs were more prone to expand on gelatin and showed a better viability than PBMCs. Moreover, about 90% of the BMMCs pre-cultured on gelatin could adhere to a hyaluronan-based scaffold and proliferate on it up to 3 days. Pre-treatment of BMMCs on fibronectin generated well-shaped tubular structures on Matrigel, whilst BMMCs exposed to the gelatin culture condition were less prone to form vessel-like structures. MSCs formed rough tubule-like structures, irrespective of the differentiating condition used. In a relative short time, pig BMMCs could be expanded on gelatin better than PBMCs, in the presence of a low amount of VEGF. BMMCs could better specialize for capillary formation in the presence of fibronectin and an elevated concentration of VEGF, whilst pig MSCs anyway showed a limited capability to differentiate into the endothelial cell lineage

    Imaging of Bubonic Plague Dynamics by In Vivo Tracking of Bioluminescent Yersinia pestis

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    Yersinia pestis dissemination in a host is usually studied by enumerating bacteria in the tissues of animals sacrificed at different times. This laborious methodology gives only snapshots of the infection, as the infectious process is not synchronized. In this work we used in vivo bioluminescence imaging (BLI) to follow Y. pestis dissemination during bubonic plague. We first demonstrated that Y. pestis CO92 transformed with pGEN-luxCDABE stably emitted bioluminescence in vitro and in vivo, while retaining full virulence. The light produced from live animals allowed to delineate the infected organs and correlated with bacterial loads, thus validating the BLI tool. We then showed that the first step of the infectious process is a bacterial multiplication at the injection site (linea alba), followed by a colonization of the draining inguinal lymph node(s), and subsequently of the ipsilateral axillary lymph node through a direct connection between the two nodes. A mild bacteremia and an effective filtering of the blood stream by the liver and spleen probably accounted for the early bacterial blood clearance and the simultaneous development of bacterial foci within these organs. The saturation of the filtering capacity of the spleen and liver subsequently led to terminal septicemia. Our results also indicate that secondary lymphoid tissues are the main targets of Y. pestis multiplication and that colonization of other organs occurs essentially at the terminal phase of the disease. Finally, our analysis reveals that the high variability in the kinetics of infection is attributable to the time the bacteria remain confined at the injection site. However, once Y. pestis has reached the draining lymph nodes, the disease progresses extremely rapidly, leading to the invasion of the entire body within two days and to death of the animals. This highlights the extraordinary capacity of Y. pestis to annihilate the host innate immune response

    Analysis of the Plant bos1 Mutant Highlights Necrosis as an Efficient Defence Mechanism during D. dadantii/Arabidospis thaliana Interaction

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    Dickeya dadantii is a broad host range phytopathogenic bacterium provoking soft rot disease on many plants including Arabidopsis. We showed that, after D. dadantii infection, the expression of the Arabidopsis BOS1 gene was specifically induced by the production of the bacterial PelB/C pectinases able to degrade pectin. This prompted us to analyze the interaction between the bos1 mutant and D. dadantii. The phenotype of the infected bos1 mutant is complex. Indeed, maceration symptoms occurred more rapidly in the bos1 mutant than in the wild type parent but at a later stage of infection, a necrosis developed around the inoculation site that provoked a halt in the progression of the maceration. This necrosis became systemic and spread throughout the whole plant, a phenotype reminiscent of that observed in some lesion mimic mutants. In accordance with the progression of maceration symptoms, bacterial population began to grow more rapidly in the bos1 mutant than in the wild type plant but, when necrosis appeared in the bos1 mutant, a reduction in bacterial population was observed. From the plant side, this complex interaction between D. dadantii and its host includes an early plant defence response that comprises reactive oxygen species (ROS) production accompanied by the reinforcement of the plant cell wall by protein cross-linking. At later timepoints, another plant defence is raised by the death of the plant cells surrounding the inoculation site. This plant cell death appears to constitute an efficient defence mechanism induced by D. dadantii during Arabidopsis infection
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