Los hongos entomopatógenos endófitos mejoran la nutrición férrica y el crecimiento vegetal

Los ascomicetos mitospóricos entomopatógenos (AME) son un componente importante de la microbiota de los ecosistemas naturales, agrícolas y forestales, donde regulan las poblaciones de insectos y ácaros fitófagos, pero además, pueden ser utilizados de forma deliberada para el control de plagas de acuerdo con distintas estrategias, incluso de forma inundativa, como micoinsecticidas. Más allá, trabajos llevados a cabo principalmente en la última década resaltan que la función ecológica de los AME sobrepasa su condición de entomopatógenos, pues sus sorprendentes relaciones con las plantas, como endófitos, en el filoplano o en la rizosfera, abren nuevos horizontes en la protección y producción vegetal. Los resultados de esta tesis doctoral ponen de manifiesto los beneficios asociados al carácter endófito y competente en la rizosfera de cepas de AME, principalmente de los géneros Beauveria y Metarhizium, sobre distintas plantas cultivadas, no sólo por su impacto en el control de plagas, sino también sobre su crecimiento y nutrición férrica en suelos calcáreos.The entomopathogenic mitosporic ascomycetes (EMA) are an important component of the microbiota of natural, agricultural and forest ecosystems, where they regulate insect populations and phytophagous mites, but can also be used deliberately for the control of pests in accordance with different strategies, including inundative release as mycoinsecticides. Furthermore, works carried out mainly in the last decade highlight that the ecological role of EMAs exceeds their status as entomopathogens, because of their surprising associations with plants, as endophytes, in the phylloplane or in the rhizosphere, open new horizons in Plant protection and Production. The results of this Doctoral Thesis show the benefits associated with the endophytic and rhizosphere competent behavior of strains of EMA, mainly of the genera Beauveria and Metarhizium, on different cultivated plants, not only for their impact on the control of pests, but also about its growth and ferric nutrition in calcareous soils

A Comparison in Physical Fitness Attributes, Physical Activity Behaviors, Nutritional Habits, and Nutritional Knowledge Between Elite Male and Female Youth Basketball Players

Background: Limited evidence exists comprehensively assessing physical fitness attributes, physical activity behaviors, nutritional habits, and nutritional knowledge according to sex in basketball players during early adolescence. Insight of this nature could be used to optimize the training process and lifestyles in young basketball players. Objective: To compare physical fitness attributes, physical activity levels, nutritional habits, and nutritional knowledge between elite male and female basketball players under 14 years of age (U-14). Methods: Twenty-three U-14 basketball players (male, n = 13 and female, n = 10) from the same elite basketball academy (Spanish Asociacion de Clubes de Baloncesto [ACB] League) participated in this study. Physical fitness attributes were assessed using a basketball-specific test battery (countermovement jump, drop jump, linear sprint, Lane Agility Drill, 505 change-of-direction, and repeated-change-of-direction tests), while physical activity levels (Physical Activity Questionnaire for Adolescents, PAQ-A), nutritional habits (Turconi questionnaire), and nutritional knowledge (Turconi questionnaire) were assessed using questionnaires. Results: Male players exhibited better physical fitness in all tests (p 0.05). Of note, a high proportion of players declared never or only sometimes eating fruit (males: 23%; females: 40%) and vegetables (males: 46%; females: 70%). In addition, relatively poor nutritional knowledge was evident in all players with the group correctly answering <50% of nutritional questions overall (4.57 +/- 1.88 out of 11 points, 42%) and according to sex (males: 4.07 +/- 2.10, 37%; females: 5.20 +/- 1.40, 47%). Conclusion: These findings emphasize the necessity to perform individualized prescription of training stimuli across sexes to optimize the physical preparedness and development of youth basketball players. Additionally, strategies such as nutrition-focused education interventions may be necessary in this population given the low consumption of fruits and vegetables, as well as the poor nutritional knowledge observed in players.The research leading to these results has received funding from la Caixa Foundation and Caja de Burgos Foundation, under agreement LCF/PR/PR18/51130008 (SS-D, DC, and JR-G

Effects of Nutrition Education Interventions in Team Sport Players. A Systematic Review

Considering nutrition education interventions have been frequently implemented in team sport athletes and have shown promising results, this study aimed to summarize the effects of nutrition education interventions on eating habits, nutrition knowledge, body composition, and physical performance in team sport athletes. A systematic review was conducted using the following databases: PubMed/MEDLINE, Web of Science and SPORTDiscus. A total of 14 studies met the inclusion criteria for the review. The methodological quality of included studies was evaluated, and each study was assessed according to the analyzed variables (i.e., eating habits, nutrition knowledge, body composition, and physical performance). Most studies showed improvements in or maintenance of variables used to indicate eating habits, nutrition knowledge, and body composition. However, limited studies examined the effect of nutrition education interventions on physical performance, with existing studies demonstrating disparate results. These findings suggest implementation of nutrition education interventions in team sport athletes could be an effective strategy to improve their eating habits, nutrition knowledge, and body composition. Due to the heterogeneity across the included studies regarding sport modality, competition level, age, and sex of the athletes investigated, as well as the intervention type adopted (i.e., online or face-to-face), it is difficult to establish optimal nutrition education interventions for each analyzed variable.The research leading to these results has received funding from “la Caixa” Foundation and Caja de Burgos Foundation, under agreement LCF/PR/PR18/51130008

Small-sided game-related physical performance is not influenced by the sprint and power performance of youth male soccer players

The aim of this study was to analyse the effects of sprint and power performance on physical fitness and small-sided game-related physical performance in youth male soccer players, using a median split analysis to separate faster and slower players, and powerful and weaker ones. Thirty youth male soccer players (age = 16.9 ± 1.4 years; height = 174.1 ± 7.1 cm; body mass = 63.1 ± 7.9 kg; % body fat = 15.5 ± 3.2) completed the following physical tests: 40 m linear sprint test, repeated sprint ability (RSA) test, countermovement jump (CMJ), horizontal jump (HJ) and a half-squat (HS) power test. In addition, players completed a 4 vs. 4 with goalkeepers small-sided game while external loads were recorded. According to their sprint and HS power performance, players were divided into fast and slow, and powerful and weak. Faster players performed better in 5 m sprint (p = 0.004, Effect Size (ES) = −1.158), 10 m sprint (p < 0.001, ES = −1.722), 40 m sprint (p < 0.001, ES = −3.268), RSAbest (p < 0.001, ES = −2.415), RSAtotal (p < 0.001, ES = −2.785), CMJ height (p = 0.032, ES = 0.823) and HJ distance (p < 0.001, ES = 1.589), but no significant differences (p > 0.05) were found in external loads during small-side games (SSG). In addition, no significant differences (p > 0.05) were observed between powerful and weaker players in the fitness tests and SSG-related physical performance. These results highlight the importance of grouping youth soccer players by their sprinting capacity to design specific and individualized training strategies and suggest that SSG-related physical performance is not influenced by their sprint or power performance

Consumo de alimentos, composición corporal y rendimiento físico en hombres y mujeres jóvenes judadores de fútbol

Introduction: Few studies have analysed the nutritional habits, body composition and physical condition of young soccer players among different categories and further differentiating between males and females. Therefore, the aim was to analyze differences in food consumption habits, body composition parameters and physical performance among young soccer players. Material and Methods: A cross-sectional study was performed during the 2019-2020 competitive season. Forty-eight soccer players (16-18 years) were classified by age and sex into three groups: under 16 and under 18 years males (U16 and U18), and under 16 years females (U16 female). Food habits, anthropometry, body composition and physical performance variables were collected. A one-way analysis of variance (ANOVA) with least significant difference post hoc comparison (Bonferroni correction) was used. Results: We found that U16 females consumed lower pork and bread and higher mollusk and shellfish than males (p&lt;0.05). U16 males consumed less fruits (p&lt;0.05) and more olive oil (p&lt;0.05) than U16 females and had lower muscle mass in lower limbs than U18 male (p&lt;0.05, ES=0.80-0.96); males showed higher muscle mass in lower limbs (p&lt;0.01, ES=1.80-2.59) and lower %fat than females (p&lt;0.01, ES=1.73); and U18 males presented higher weight than females (p&lt;0.01, ES=1.64). U18 males showed better performances (p&lt;0.05-0.01, ES=0.90-1.38) in maximal sprint trials (40-m length), counter movements jumps (CMJ) and CMJ with dominant leg, maximal bilateral horizontal jumps (HJ), HJ with dominant and non-dominant leg than U16 males. Males showed better results in linear straight sprint test (LSST, p&lt;0.05; ES=1.66-5.16), change of direction ability (CODA, p&lt;0.05; ES=2.82-5.46) and jumping (p&lt;0.05; ES=1.60-4.12) performances. Conclusion: In conclusion, sex- and age- specific differences were detected in the healthier dietary practices and body composition parameter in youth of soccer players. Also, better physical performances were found as age increases, except for CODA, and males exhibited higher performances in LSST, CODA and jumping than females.Introducción: Pocos estudios han sido encontrados donde se analicen los hábitos nutricionales, la composición corporal y la condición física de jóvenes futbolistas entre diferentes categorías y diferenciando entre chicos y chicas. Por tanto, el objetivo fue analizar las diferencias en los hábitos de consumo de alimentos, los parámetros de composición corporal y el rendimiento físico en jóvenes futbolistas. Material y métodos: Se realizó un estudio transversal durante la temporada competitiva 2019-2020. Cuarenta y ocho futbolistas (16-18 años) fueron clasificados por edad y sexo en tres grupos: menores de 16 y menores de 18 años masculinos (U16 y U18), y menores de 16 años féminas (U16 femenino). Se recogieron variables de hábitos alimentarios, antropometría, composición corporal y rendimiento físico. Se utilizó un análisis de varianza de (ANOVA) de un factor con post hoc de Bonferroni. Resultados: Observamos que las chicas U16 consumieron menos carne de cerdo y pan y más moluscos y mariscos que los chicos (p&lt;0,05). Los chicos U16 consumieron menos frutas (p&lt;0,05) y más aceite de oliva (p&lt;0,05) que las chicas U16 y tuvieron menor masa muscular en miembros inferiores que los chicos U18 (p &lt;0,05, ES=0,80-0,96); los chicos mostraron mayor masa muscular en miembros inferiores (p &lt;0,01, ES=1,80-2,59) y menor porcentaje de grasa que las chicas (p&lt;0,01, ES=1,73); y los chicos U18 presentaron mayor peso que las chicas (p&lt;0,01, ES=1,64). Los chicos U18 mostraron mejores rendimientos (p&lt;0,05-0,01, ES=0,90-1,38) en pruebas de sprint máximo (40 m de longitud), salto en contramovimiento (CMJ) y CMJ con pierna dominante, saltos horizontales bilaterales máximos (HJ), HJ con pierna dominante y no dominante que los chicos U16. Los chicos mostraron mejores resultados en los esprints (LSST, p&lt;0,05; ES=1,66-5,16), el cambio de dirección (CODA, p&lt;0,05; ES = 2,82-5,46) y los saltos (p&lt;0,05; ES=1,60-4,12). Conclusión: En conclusión, se observaron diferencias por sexo y edad en las prácticas dietéticas y los parámetros de composición corporal en los jóvenes futbolistas. Además, mejor rendimiento físico a medida que aumentaba la edad, excepto para el CODA. Los chicos exhibieron mejores rendimientos en LSST, CODA y saltos que las chicas

Model del Programa d’atenció domiciliària (ATDOM) de l’atenció primària i comunitària (APiC)

Atenció domiciliària; Atenció primària i comunitària; Cartera de serveisHome care; Primary and community care; Portfolio of servicesAtención domiciliaria; Atención primaria y comunitaria; Cartera de serviciosAquest document defineix les bases del Programa d’atenció domiciliària (ATDOM) de l’atenció primària i comunitària a Catalunya: els principis en els quals s’inspira, la cartera de serveis, uns elements clau en la prestació i els resultats esperats, el seguiment i l’avaluació

Complement component C4 structural variation and quantitative traits contribute to sex-biased vulnerability in systemic sclerosis

Altres ajuts: Fondo Europeo de Desarrollo Regional (FEDER), "A way of making Europe".Copy number (CN) polymorphisms of complement C4 play distinct roles in many conditions, including immune-mediated diseases. We investigated the association of C4 CN with systemic sclerosis (SSc) risk. Imputed total C4, C4A, C4B, and HERV-K CN were analyzed in 26,633 individuals and validated in an independent cohort. Our results showed that higher C4 CN confers protection to SSc, and deviations from CN parity of C4A and C4B augmented risk. The protection contributed per copy of C4A and C4B differed by sex. Stronger protection was afforded by C4A in men and by C4B in women. C4 CN correlated well with its gene expression and serum protein levels, and less C4 was detected for both in SSc patients. Conditioned analysis suggests that C4 genetics strongly contributes to the SSc association within the major histocompatibility complex locus and highlights classical alleles and amino acid variants of HLA-DRB1 and HLA-DPB1 as C4-independent signals

Integrative epigenomics in Sjögren´s syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signature

Primary Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by lymphocytic infiltration and damage of exocrine salivary and lacrimal glands. The etiology of SS is complex with environmental triggers and genetic factors involved. By conducting an integrated multi-omics study, we confirmed a vast coordinated hypomethylation and overexpression effects in IFN-related genes, what is known as the IFN signature. Stratified and conditional analyses suggest a strong interaction between SS-associated HLA genetic variation and the presence of Anti-Ro/SSA autoantibodies in driving the IFN epigenetic signature and determining SS. We report a novel epigenetic signature characterized by increased DNA methylation levels in a large number of genes enriched in pathways such as collagen metabolism and extracellular matrix organization. We identified potential new genetic variants associated with SS that might mediate their risk by altering DNA methylation or gene expression patterns, as well as disease-interacting genetic variants that exhibit regulatory function only in the SS population. Our study sheds new light on the interaction between genetics, autoantibody profiles, DNA methylation and gene expression in SS, and contributes to elucidate the genetic architecture of gene regulation in an autoimmune population

Global phylogeography and ancient evolution of the widespread human gut virus crAssphage

Microbiomes are vast communities of microorganisms and viruses that populate all natural ecosystems. Viruses have been considered to be the most variable component of microbiomes, as supported by virome surveys and examples of high genomic mosaicism. However, recent evidence suggests that the human gut virome is remarkably stable compared with that of other environments. Here, we investigate the origin, evolution and epidemiology of crAssphage, a widespread human gut virus. Through a global collaboration, we obtained DNA sequences of crAssphage from more than one-third of the world's countries and showed that the phylogeography of crAssphage is locally clustered within countries, cities and individuals. We also found fully colinear crAssphage-like genomes in both Old-World and New-World primates, suggesting that the association of crAssphage with primates may be millions of years old. Finally, by exploiting a large cohort of more than 1,000 individuals, we tested whether crAssphage is associated with bacterial taxonomic groups of the gut microbiome, diverse human health parameters and a wide range of dietary factors. We identified strong correlations with different clades of bacteria that are related to Bacteroidetes and weak associations with several diet categories, but no significant association with health or disease. We conclude that crAssphage is a benign cosmopolitan virus that may have coevolved with the human lineage and is an integral part of the normal human gut virome

Albiglutide and cardiovascular outcomes in patients with type 2 diabetes and cardiovascular disease (Harmony Outcomes): a double-blind, randomised placebo-controlled trial

Background: Glucagon-like peptide 1 receptor agonists differ in chemical structure, duration of action, and in their effects on clinical outcomes. The cardiovascular effects of once-weekly albiglutide in type 2 diabetes are unknown. We aimed to determine the safety and efficacy of albiglutide in preventing cardiovascular death, myocardial infarction, or stroke. Methods: We did a double-blind, randomised, placebo-controlled trial in 610 sites across 28 countries. We randomly assigned patients aged 40 years and older with type 2 diabetes and cardiovascular disease (at a 1:1 ratio) to groups that either received a subcutaneous injection of albiglutide (30–50 mg, based on glycaemic response and tolerability) or of a matched volume of placebo once a week, in addition to their standard care. Investigators used an interactive voice or web response system to obtain treatment assignment, and patients and all study investigators were masked to their treatment allocation. We hypothesised that albiglutide would be non-inferior to placebo for the primary outcome of the first occurrence of cardiovascular death, myocardial infarction, or stroke, which was assessed in the intention-to-treat population. If non-inferiority was confirmed by an upper limit of the 95% CI for a hazard ratio of less than 1·30, closed testing for superiority was prespecified. This study is registered with ClinicalTrials.gov, number NCT02465515. Findings: Patients were screened between July 1, 2015, and Nov 24, 2016. 10 793 patients were screened and 9463 participants were enrolled and randomly assigned to groups: 4731 patients were assigned to receive albiglutide and 4732 patients to receive placebo. On Nov 8, 2017, it was determined that 611 primary endpoints and a median follow-up of at least 1·5 years had accrued, and participants returned for a final visit and discontinuation from study treatment; the last patient visit was on March 12, 2018. These 9463 patients, the intention-to-treat population, were evaluated for a median duration of 1·6 years and were assessed for the primary outcome. The primary composite outcome occurred in 338 (7%) of 4731 patients at an incidence rate of 4·6 events per 100 person-years in the albiglutide group and in 428 (9%) of 4732 patients at an incidence rate of 5·9 events per 100 person-years in the placebo group (hazard ratio 0·78, 95% CI 0·68–0·90), which indicated that albiglutide was superior to placebo (p&lt;0·0001 for non-inferiority; p=0·0006 for superiority). The incidence of acute pancreatitis (ten patients in the albiglutide group and seven patients in the placebo group), pancreatic cancer (six patients in the albiglutide group and five patients in the placebo group), medullary thyroid carcinoma (zero patients in both groups), and other serious adverse events did not differ between the two groups. There were three (&lt;1%) deaths in the placebo group that were assessed by investigators, who were masked to study drug assignment, to be treatment-related and two (&lt;1%) deaths in the albiglutide group. Interpretation: In patients with type 2 diabetes and cardiovascular disease, albiglutide was superior to placebo with respect to major adverse cardiovascular events. Evidence-based glucagon-like peptide 1 receptor agonists should therefore be considered as part of a comprehensive strategy to reduce the risk of cardiovascular events in patients with type 2 diabetes. Funding: GlaxoSmithKline