Enhancing methane production from food waste fermentate using biochar. The added value of electrochemical testing in pre-selecting the most effective type of biochar

Background: Recent studies have suggested that addition of electrically conductive biochar particles is an effective strategy to improve the methanogenic conversion of waste organic substrates, by promoting syntrophic associations between acetogenic and methanogenic organisms based on interspecies electron transfer processes. However, the underlying fundamentals of the process are still largely speculative and, therefore, a priori identification, screening, and even design of suitable biochar materials for a given biotechnological process are not yet possible. Results: Here, three charcoal-like products (i.e., biochars) obtained from the pyrolysis of different lignocellulosic materials, (i.e., wheat bran pellets, coppiced woodlands, and orchard pruning) were tested for their capacity to enhance methane production from a food waste fermentate. In all biochar-supplemented (25 g/L) batch experiments, the complete methanogenic conversion of fermentate volatile fatty acids proceeded at a rate that was up to 5 times higher than that observed in the unamended (or sand-supplemented) controls. Fluorescent in situ hybridization analysis coupled with confocal laser scanning microscopy revealed an intimate association between archaea and bacteria around the biochar particles and provided a clear indication that biochar also shaped the composition of the microbial consortium. Based on the application of a suite of physico-chemical and electrochemical characterization techniques, we demonstrated that the positive effect of biochar is directly related to the electron-donating capacity (EDC) of the material, but is independent of its bulk electrical conductivity and specific surface area. The latter properties were all previously hypothesized to play a major role in the biochar-mediated interspecies electron transfer process in methanogenic consortia. Conclusions: Collectively, these results of this study suggest that for biochar addition in anaerobic digester operation, the screening and identification of the most suitable biochar material should be based on EDC determination, via simple electrochemical tests. © 2017 The Author(s)

Co-composted biochar improves barley yield, manure use efficiency and offsets chemical fertilizer demand in organic agriculture under low rainfall conditions

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Fate of soil organic carbon and polycyclic aromatic hydrocarbons in a vineyard soil treated with biochar

The effect of biochar addition on the levels of black carbon (BC) and polcyclic aromatic hydrocarbons (PAHs) in a vineyard soil in central Italy was investigated within a two year period. Hydropyrolysis (HyPy) was used to determine the contents of BC (BCHyPy) in the amended and control soils while the hydrocarbon composition of the semi-labile (non-BCHyPy) fraction released by HyPy was determined by gas chromatography-mass spectrometry, together with the solvent-extractable PAHs. The concentrations of these three polycyclic aromatic carbon reservoirs, changed and impacted differently on the soil organic carbon over the period of the trial. The addition of biochar (33 ton dry biochar ha-1) gave rise to a sharp increase in soil organic carbon which could be accounted for by an increase of BCHyPy. Over time, the concentration of BCHyPy decreased significantly from 36 to 23 mg g-1, and as a carbon percentage from 79% to 61%. No clear time trends were observed for the non-BCHyPy PAHs varying from 39 to 34 µg g-1 in treated soils, not significantly different from control soils. However, the concentrations of extractable PAHs increased markedly in the amended soils, and decreased with time from 153 to 78 ng g-1 remaining always higher than those in untreated soil. The extent of the BCHyPy loss was more compatible with physical rather than chemical processes

The potential of recycling wool residues as an amendment for enhancing the physical and hydraulic properties of a sandy loam soil

Climate change and global food demand in coming decades urge effective actions for more efficient uses of water and soil resources. This paper reports the preliminary findings of a study assessing the potential of sheep scoured wool residues (SWRs) as soil amendments to enhance the physical and hydraulic properties of a sandy loam soil under rain conditions. Methods: Two different SWRs were used: scoured residues (white wool, WW) and carbonized scoured residues (black wool, BW) at different SWRs/soil ratios (0.0, 0.5, 1.0 and 2.0%). Soil bulk density (BD), total porosity (TP), aggregates stability, aggregate size distribution, saturated hydraulic conductivity, and water retention properties were determined under rain conditions, in addition to rainwater balance (storage, percolation and runoff). Results: Both WW and BW, particularly at the high wool/soil ratio (2%), significantly reduced soil BD by 11.98% and 9.85%, respectively. Moreover, WW and BW increased TP by 16.45% and 13.57% and available water capacity by 6.5% and 18.1%, respectively. SWRs increased the formation of macro-aggregates and increased aggregate stability. The results of rainwater balance showed higher percolation percentages and less rainwater storage in the wool-treated soil. Conclusions: The increase in water percolation is in line with the increased total porosity and the higher saturated hydraulic conductivity of wool-treated soil. Despite the high capacity of absorbing water, SWRs affected the water movement of the soil more than its water retention

The effects of biochar and its combination with compost on lettuce (Lactuca sativa L.) growth, soil properties, and soil microbial activity and abundance

Impacts of biochar application in combination with organic fertilizer, such as compost, are not fully understood. In this study, we tested the effects of biochar amendment, compost addition, and their combination on lettuce plants grown in a soil poor in nutrients; soil microbiological, chemical, and physical characteristics were analyzed, together with plant growth and physiology. An initial screening was also done to evaluate the effect of biochar and compost toxicity, using cress plants and earthworms. Results showed that compost amendment had clear and positive effects on plant growth and yield and on soil chemical characteristics. However, we demonstrated that also the biochar alone stimulated lettuce leaves number and total biomass, improving soil total nitrogen and phosphorus contents, as well as total carbon, and enhancing related microbial communities. Nevertheless, combining biochar and compost, no positive synergic and summative effects were observed. Our results thus demonstrate that in a soil poor in nutrients the biochar alone could be effectively used to enhance soil fertility and plant growth and biomass yield. However, we can speculate that the combination of compost and biochar may enhance and sustain soil biophysical and chemical characteristics and improve crop productivity over time

A multi-domain ontology on healthy ageing for the characterization of older adults status and behaviour

Ageing is a multi-factorial physiological process and the development of novel IoT systems, tools and devices, specifically targeted to older people, must be based on a holistic framework built on robust scientific knowledge in different health domains. Furthermore, interoperability must be guaranteed using standardized frameworks or approaches. These aspects still largely lack in the specific literature. The main aim of the paper is to develop a new ontology (the NESTORE ontology) to extend the available ontologies provided by universAAL-IoT (uAAL-IoT). The ontology is based on a multidomain healthy ageing holistic model, structuring well-assessed scientific knowledge, specifically targeted to healthy older adults aged between 65 and 75. The tool is intended to support, and standardize heterogeneous data about ageing in compliance with the uAAL-IoT framework. The NESTORE ontology covers all the relevant concepts to represent 3 significant domains of ageing: (1) Physiological Status and Physical Activity Behaviour; (2) Nutrition; and (3) Cognitive and Mental Status and Social Behaviour. In total, 12 sub-ontologies were modelled with more than 60 classes and sub-classes referenced among them by using more than 100 relations and around 20 enumerations. The proposed ontology increases the uAAL collection by 40%. NESTORE ontology provides innovation both in terms of semantic content and technological approach. The thorough use of this ontology can support the development of a decision support system, to promote healthy ageing, with the capacity to do dynamic multi-scale modelling of user-specific data based on the semantic annotations of users’ profile

Retrieving soil moisture in rainfed and irrigated fields using Sentinel-2 observations and a modified OPTRAM approach

Abstract Surface soil water content plays an important role in driving the exchange of latent and sensible heat between the atmosphere and land surface through transpiration and evaporation processes, regulating key physiological processes affecting plants growth. Given the high impact of water scarcity on yields, and of irrigated agriculture on the overall withdrawal rate of freshwater, it is important to define models that help to improve water resources management for agricultural purposes, and to optimize rainfed crop yield. Recent advances in satellite-based remote sensing have led to valuable solutions to estimate soil water content based on microwave or optical/thermal-infrared data. This study aims at improving soil water content estimation at high spatial and temporal resolution, by means of the Optical Trapezoid Model (OPTRAM) driven by Copernicus Sentinel-2 data. Two different model variations were considered, based on linear and nonlinear parameters constraints, and validated against in situ soil water content measurements made with time domain reflectometry (TDR) on irrigated maize in central Italy and on rainfed maize and pasture in northern Italy. For the first site the non-linear model shows a better correlation between measured and estimated soil water content values (r = 0.80) compared to the linear model (r = 0.73). In both cases the modeled soil moisture tends to overestimate the measured values at medium to high water content level, while both models underestimate soil moisture at low water content level. Estimated versus measured normalized surface soil water for rainfed pasture plots from nonlinear OPTRAM parametrized based on irrigated maize parameterization (SIM1), and site-specific parametrization for rainfed pasture (SIM2), indicate that both models (SIM1 and SIM2) are comparable for rotational grazing pasture (RMSEsim1 = 0.0581 vs. RMSEsim2 = 0.0485 cm3 cm-3) and the continuous grazing pasture (RMSEsim1 = 0.0485 vs. RMSEsim2 = 0.0602 cm3 cm-3), while for the rainfed maize plots SIM1 shows lower RMSE (average for all plots RMSE = 0.0542 cm3 cm-3) compared to the site-specific calibration model (SIM2 – average for all plots RMSE = 0.0645 cm3 cm-3). Finally, OPTRAM estimations are close to in situ measurement values while Surface Soil Moisture at 1 km (SSM1 km) tends to underestimate the measurements during maize crop growing season. Soil moisture retrieval from high-resolution Sentinel-2 optical images allows water stress conditions to be effectively mapped, supporting decision making in irrigation scheduling and other crop management

Biochar successfully replaces activated charcoal for in vitro culture of two white poplar clones reducing ethylene concentration

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Plant Growth in LED-Sourced Biophilic Environments Is Improved by the Biochar Amendment of Low-Fertility Soil, the Reflection of Low-Intensity Light, and a Continuous Photoperiod

Introducing plants in the design of biophilic indoor environments is fundamental for improving human health, well-being, and performance. Previous studies showed that the phenotype of the model plant Arabidopsis thaliana grown under LED-sourced CoeLux® lighting systems was characterized by low biomass production rates, a small leaf area, and a low lamina-to-petiole length ratio, suggesting the onset of a strong shade avoidance syndrome. Therefore, it is essential to identify new strategies to improve plant growth under these peculiar light conditions. In the present work, we investigated the effects of two growing media (i.e., low-fertility soil and soil-less substrate), solid and liquid fertilizers, manure, biochar, perlite, mirror reflection of light, and a 24 h photoperiod on A. thaliana plants growing under CoeLux® lighting systems at a light intensity of 30 μmol m−2s−1. We found that the biochar soil amendment to low-fertility soil increases both the above-ground plant biomass and leaf area. Furthermore, the application of a mirror behind the plants and a continuous photoperiod improves not only the biomass and the leaf area but also the lamina-to-petiole length ratio. The combination of different beneficial treatments can further boost plant growth in the low-intensity light environment characterizing the CoeLux® biophilic lighting systems