VIVER ATIVO: RELATO DE EXPERIÊNCIA EM ATIVIDADE FÍSICA COM IDOSOS NO MUNICÍPIO DE PARINTINS-AM.

Este trabalho relata a experincia de um projeto de extenso universitria, cujo objetivo foi promover atividades fsicas direcionadas aos idosos participantes do Programa Viva Mais, oferecido pela Secretaria Municipal de Assistncia Social do municpio de Parintins/AM. As aes foram desenvolvidas no perodo de seis meses, sendo divididas em cinco etapas distintas: capacitao/educao permanente, observao participante, planejamento das atividades fsicas, oferta de atividades fsicas e evento de socializao com os idosos. Notou-se que os idosos responderam de forma positiva as prticas de sade e educao desenvolvidas, sempre motivados a participar. Aos acadmicos rendeu-lhes conhecimento terico-prtico e vivncias impactantes para a carreira acadmica, tendo em vista que nas diversas situaes foi ofertado vivncias que foram discutido em reunies assim como alguns acontecimento por vezes foram inesperados outros surpreendente. Dessa forma, houve grande conhecimento e aprendizagem aos acadmicos do curso de Licenciatura em Educao Fsica do ICSEZ/UFAM envolvidos no projeto. Deixamos como sugesto daquilo que foi observado e vivido, que mas instituies sejam elas governamentais ou de ensino possam oportunizar projetos as pessoas de terceira idade para contribuir com a promoo da sade. Palavras-chave: Idoso; Atividade Fsica; Promoo da sade

Prevenção da negligência e violência contra a criança e adolescente : para ensinar e aprender

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Seguimento clínico de dois pacientes brasileiros com MODY-glicoquinase (MODY2) e descrição de uma nova mutação

Mutations in the glucokinase gene (GCK) account for many cases of monogenic diabetes featuring maturity-onset diabetes of the young type 2 (MODY2). The clinical pattern of this form of hyperglycemia is rather stable, with a slight elevation in blood glucose, which is usually not progressive. Patients rarely require pharmacological interventions and microvascular complications related to diabetes are unusual. We describe the clinical follow-up of two cases of MODY2 with two different mutations in GCK gene, one in exon 7, p.Glu265Lys (c.793 G> A), which has been previously described, and a novel one, in exon 2, p.Ser69Stop (c. 206C> G). The clinical course of both cases shows similarity in metabolic control of this form of diabetes over the years. Arq Bras Endocrinol Metab. 2012;56(8):490-5Mutações no gene da glicoquinase (GCK) são determinantes de uma forma de diabetes monogênico denominada de MODY2 (maturity-onset diabetes of the young, tipo 2). O padrão clínico dessa forma de distúrbio glicêmico é bastante estável, com hiperglicemia leve, geralmente não progressiva. Intervenções farmacológicas raramente são necessárias e complicações crônicas secundárias ao diabetes são infrequentes. Descrevemos o acompanhamento clínico de dois casos de MODY2 com duas mutações diferentes, uma no éxon 7, p.Glu265Lys (c.793 G>A) já descrita anteriormente, e outra inédita no éxon 2 p.Ser69Stop (c. 206C>G). A evolução clínica de ambos os casos demonstra uma semelhança no padrão metabólico dessa forma de diabetes ao longo dos anos. Arq Bras Endocrinol Metab. 2012;56(8):490-5Faculdade de Medicina da Universidade de São Paulo Hospital das Clínicas Instituto da CriançaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratório de Endocrinologia Molecular e TranslacionalUNIFESP-EPM Centro de DiabetesUNIFESP, EPM, Laboratório de Endocrinologia Molecular e TranslacionalUNIFESP, EPM Centro de DiabetesSciEL

The Extracellular Matrix of the Lateral Pharyngeal Wall in Obstructive Sleep Apnea

Study Objectives: To compare the components of the extracellular matrix in the lateral pharyngeal muscular wall in patients with and without obstructive sleep apnea (OSA). This may help to explain the origin of the increased collapsibility of the pharynx in patients with OSA. Design: Specimens from the superior pharyngeal constrictor muscle, obtained during pharyngeal surgeries, were evaluated using histochemical and immunohistochemical analyses to determine the fractional area of collagen types I and II, elastic fibers, versican, fibronectin, and matrix metalloproteinases 1 and 2 in the endomysium. Setting: Academic tertiary center. Patiens: A total of 51 nonobese adult patients, divided into 38 patients with OSA and 13 nonsnoring control subjects without OSA. Interventions: Postintervention study performed on tissues from patients after elective surgery. Measurements and Results: Pharyngeal muscles of patients with OSA had significantly more collagen type I than pharyngeal muscles in control subjects. Collagen type I was correlated positively and independently with age. The other tested components of the extracellular matrix did not differ significantly between groups. In a logistic regression, an additive effect of both the increase of collagen type I and the increase in age with the presence of OSA was observed (odds ratio (OR), 2.06; 95% confidence interval (CI), 1.17-3.63), when compared with the effect of increased age alone (OR, 1.11; 95% CI, 1.03-1.20). Conclusion: Collagen type I in the superior pharyngeal constrictor muscle was more prevalent in patients with OSA and also increased with age. It was hypothesized that this increase could delay contractile-relaxant responses in the superior pharyngeal constrictor muscle at the expiratory-inspiratory phase transition, thus increasing pharyngeal collapsibility.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [06/50630-7

Electrochemical Behavior of Cellulose Nanofibrils Functionalized with Dicyanovinyl Groups

Cellulose is considered one of the most important renewable sources of biopolymers on Earth. It has attracted widespread attention due to its physical–chemical characteristics, such as biocompatibility, low toxicity, biodegradability, low density, high strength, stability in organic solvents, in addition to having hydroxyl groups, which enable its chemical modification. In this study, cellulose nanofibrils (CNFs) were functionalized with dicyanovinyl groups through nucleophilic vinylic substitution (SNV) and used as electrocatalyst in electrochemical of carbon dioxide (CO2) reduction. Results indicate that introducing dicyanovinyl groups into the structure of nanocellulose increases electrocatalytic activity as compared to that of pure nanocellulose, shifting the onset potential of the electrochemical CO2 reduction reaction to more positive values as compared to those for the reaction with argon. The atomic force microscopy (AFM) images show no changes in the morphology of CNFs after chemical modification

Is intrauterine exposure to acetaminophen associated with emotional and hyperactivity problems during childhood? : findings from the 2004 Pelotas birth cohort

Background: Longitudinal studies have consistently reported that prenatal exposure to acetaminophen can to lead to an increased risk of attention deficit-hyperactivity disorder during childhood. This study aimed to investigate the association between intrauterine exposure to acetaminophen and the presence of emotional and behavioral problems at the ages of 6 and 11 years in a low-middle income country. Methods: We performed a prospective longitudinal population-based study using data from the 2004 Pelotas birth cohort. From the 4231 initial cohort participants, 3722 and 3566 children were assessed at 6 and 11 years of age, respectively. The outcomes were assessed using the parent version of Strengths and Difficulties Questionnaire (SDQ). The cut-off points established for the Brazilian population were used to categorize the outcomes. Crude and adjusted odds ratio were obtained through logistic regression. Results: Acetaminophen was used by 27.5% (95% confidence interval [CI]: 26.1–28.9) of the mothers at least once during pregnancy. The prevalence of emotional problems at 6 and 11 years was 13.6 and 19.9%, respectively. For hyperactivity problems, prevalence was 13.9 and 16.1%, respectively. Intrauterine exposure to acetaminophen increased the odds of having emotional (odds ratio [OR] = 1.47; 95% CI: 1.07–2.02) and hyperactivity/inattention (OR = 1.42; 95% CI: 1.06–1.92) problems in 6-year-old boys. At the age of 11, a small decrease in the effect was observed for both outcomes after adjustment: OR = 1.31 (95% CI: 0.99–1.73) for emotional problems and OR = 1.25 (95% CI: 0.95–1.65) for hyperactivity/inattention in boys. No association for any phenotypes at both ages was observed for girls. Conclusion: The effect of intrauterine exposure to acetaminophen in emotional and hyperactivity symptoms was dependent on sex in a Brazilian cohort. While it seemed to be important for boys, mainly at 6 years of age, for girls, no association was observed

Zika Virus Disrupts Molecular Fingerprinting Of Human Neurospheres

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human brain development are still not fully understood. Here we describe alterations in human neurospheres derived from induced pluripotent stem (iPS) cells infected with the strain of Zika virus that is circulating in Brazil. Combining proteomics and mRNA transcriptional profiling, over 500 proteins and genes associated with the Brazilian ZIKV infection were found to be differentially expressed. These genes and proteins provide an interactome map, which indicates that ZIKV controls the expression of RNA processing bodies, miRNA biogenesis and splicing factors required for self-replication. It also suggests that impairments in the molecular pathways underpinning cell cycle and neuronal differentiation are caused by ZIKV. These results point to biological mechanisms implicated in brain malformations, which are important to further the understanding of ZIKV infection and can be exploited as therapeutic potential targets to mitigate it.7Brazilian Development Bank (BNDES)Funding Authority for Studies and Projects (FINEP)National Council of Scientific and Technological Development (CNPq)Foundation for Research Support in the State of Rio de Janeiro (FAPERJ)Sao Paulo Research Foundation (FAPESP) [14/21035-0, 14/14881-1, 13/08711-3, 14/10068-4]Coordination for the Improvement of Higher Education Personnel (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Iron and Nickel spectral opacity calculations in conditions relevant for pulsating stellar envelopes and experiments

Seismology of stars is strongly developing. To address this question we have formed an international collaboration OPAC to perform specific experimental measurements, compare opacity calculations and improve the opacity calculations in the stellar codes [1]. We consider the following opacity codes: SCO, CASSANDRA, STA, OPAS, LEDCOP, OP, SCO-RCG. Their comparison has shown large differences for Fe and Ni in equivalent conditions of envelopes of type II supernova precursors, temperatures between 15 and 40 eV and densities of a few mg/cm3 [2, 3, 4]. LEDCOP, OPAS, SCO-RCG structure codes and STA give similar results and differ from OP ones for the lower temperatures and for spectral interval values [3]. In this work we discuss the role of Configuration Interaction (CI) and the influence of the number of used configurations. We present and include in the opacity code comparisons new HULLAC-v9 calculations [5, 6] that include full CI. To illustrate the importance of this effect we compare different CI approximations (modes) available in HULLAC-v9 [7]. These results are compared to previous predictions and to experimental data. Differences with OP results are discussed.Comment: 4 pages, 3 figures, conference Inertial Fusion Sciences and Applications, Bordeaux, 12th to 16th September 2011; EPJ web of Conferences 201

MAPEAMENTO DE VOÇOROCAS E DO RISCO DE EROSÃO EM ÁREA URBANA NO OESTE DO ESTADO DO MARANHÃO

Dentre os fatores de erosão, a erodibilidade combinada a um relevo irregular, assume posição de destaque e determina o grau de susceptibilidade dos solos a serem erodidos. Por outro lado, as atividades desenvolvidas pelo homem alteram a dinâmica desse processo, acelerando suas ações. Em Buriticupu, município situado na porção centro oeste do Estado do Maranhão, os solos já tiveram sua erodibilidade elevada mencionada em diversos trabalhos, porém nenhum estudo que determinem valores para os atributos químicos e físicos dos solos foi elaborado e publicado. Neste sentido, a presente pesquisa objetivou identificar e mapear os processos erosivos por voçorocamento na sede municipal de Buriticupu – MA. O município sofre com os problemas erosivos, já sendo percebidas voçorocas que fizeram desaparecer residências e que aumentam sua ação, em especial no período mais intenso das chuvas. As atividades humanas associadas à impermeabilização de vias e condução das águas pluviais e esgotos para as encostas sem canal escoadouro, galeria ou tubulação, associadas ao uso e ocupação das encostas são atividades que aceleram intensamente a erosão, fato que tem causado danos ambientais e sociais das comunidades situados no entorno das inúmeras voçorocas situadas em Buriticupu

What is the response profile of deciduous pulp fibroblasts stimulated with E. coli LPS and E. faecalis LTA?

BACKGROUND: Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. METHODS: Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not with Escherichia coli lipopolysaccharide/1 μg/mL (EcLPS) or Enterococcus faecalis lipoteichoic acid/1 μg/mL (EfLTA) for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX®, respectively, for Interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-α), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-α), Interferon-gamma (IFN γ), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). RESULTS: EcLPS increased IL-1α, IL-1β, IL-8, CCL2, CCL5, TNF-α and CSF-1 mRNA and protein levels while EfLTA was only able to positively regulate gene expression and protein production of IL-8. CONCLUSION: The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated with EcLPS and EfLTA