A Quantitative Study of the Division Cycle of Caulobacter crescentus Stalked Cells

Progression of a cell through the division cycle is tightly controlled at different steps to ensure the integrity of genome replication and partitioning to daughter cells. From published experimental evidence, we propose a molecular mechanism for control of the cell division cycle in Caulobacter crescentus. The mechanism, which is based on the synthesis and degradation of three “master regulator” proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of α-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine

Parenting and peer-group behavior in cultural context

© Cambridge University Press 2006 and Cambridge University Press, 2009. Whether specific patterns of parenting are similarly associated with child peer group behavior in diverse cultural contexts has been a fascinating topic of inquiry. From classic anthropological studies dating back to the early twentieth century to the current interest in cross-cultural studies, knowledge concerning the question of universality and cultural variation in parenting linkages to childhood adjustment has expanded at an unprecedented rate (e.g., Harkness & Super, 2002). As the general field of parenting research has uncovered distinctions in parenting styles and practices (e.g., Darling & Steinberg, 1993; Hart, Newell, & Olsen, 2003), these concepts have increasingly been applied to other cultures as well. Furthermore, the study of peer relationships has also increased in complexity. For example, descriptions of social behavior have evolved to represent significant subtypes of childhood aggression (e.g., physical and relational) and peer withdrawal (e.g., reticence, solitary-passive, solitary-active). In this chapter, we highlight cultural commonalities and variations in parenting and certain child peer group behaviors that have emerged from recent studies conducted in a number of cultures around the world. For example, our own collaborative work represents cultures of Adelaide, Australia; Beijing, China; Voronezh, Russia; as well as Provo, Utah, and Baton Rouge, Louisiana (United States). As a whole, these research endeavors uniquely contribute to cross-cultural developmental science. More often than not, a relatively coherent picture regarding parenting and child outcomes is emerging from numerous cultural studies. The structure of this chapter is as follows

Relations between Chinese mothers\u27 parenting practices and social withdrawal in early childhood

Researchers have identified specific parenting practices used by parents of preschoolers in mainland China (e.g., physical coercion, overprotection, shaming, directiveness, encouragement of modesty). Some of the intrusive practices have been linked to social withdrawal in western societies (e.g., United States, Canada). It seemed important to examine these associations in China because recent research suggests that young Chinese children who exhibit wariness in peer settings may be at risk for negative outcomes such as peer rejection. Therefore, the purpose of this study was to examine the relation between Chinese parenting practices and preschoolers\u27 social withdrawal. Mothers of preschool-age children from mainland China (N = 446) completed self-report parenting questionnaires. Teachers rated children\u27s reticent, solitary-passive, solitary-active, and modest behaviors. Results showed that (a) maternal directiveness was positively associated with reticent behavior in girls and negatively associated with solitary-passive behavior in boys, (b) maternal overprotection, for girls, was positively related to both reticent behavior and solitary-passive behavior, and negatively related to modest behavior, (c) coercion was positively associated with solitary-active and reticent behavior in girls, and (d) shaming was positively related to all forms of withdrawn behaviors in boys and girls, as well as positively related to modest behavior in boys. © 2006 The International Society for the Study of Behavioural Development

PRISM: A Non-Equilibrium, Multiphase Interstellar Medium Model for Radiation Hydrodynamics Simulations of Galaxies

We introduce the PRISM interstellar medium (ISM) model for thermochemistry and its implementation in the RAMSES-RTZ code. The model includes a non-equilibrium primordial, metal, and molecular chemistry network for 115 species coupled to on-the-fly multifrequency radiation transport. PRISM accurately accounts for the dominant ISM cooling and heating processes in the low-density regime (i.e. $\rho<10^5\ {\rm cm^{-3}}$), including photoheating, photoelectric heating, H$_2$ heating/cooling, cosmic-ray heating, H/He cooling, metal-line cooling, CO cooling, and dust cooling (recombination and gas-grain collisions). We validate the model by comparing 1D equilibrium simulations across six dex in metallicity to existing 1D ISM models in the literature. We apply PRISM to high-resolution (4.5 pc) isolated dwarf galaxy simulations that include state-of-the-art models for star formation and stellar feedback to take an inventory of which cooling and heating processes dominate each different gas phase of a galaxy and to understand the importance of non-equilibrium effects. We show that most of the ISM gas is either close to thermal equilibrium or exhibits a slight cooling instability, while from a chemical perspective, the non-equilibrium electron fraction is often more than three times higher or lower than the equilibrium value, which impacts cooling, heating, and observable emission lines. Electron enhancements are attributed to recombination lags while deficits are shown to be due to rapid cosmic-ray heating. The PRISM model and its coupling to RAMSES-RTZ is applicable to a wide variety of astrophysical scenarios, from cosmological simulations to isolated giant molecular clouds, and is particularly useful for understanding how changes to ISM physics impact observable quantities such as metallic emission lines.Comment: 21 pages, 16 figures, submitted to MNRA

Novel neuronal surface autoantibodies in plasma of patients with depression and anxiety

Neuronal surface autoantibodies (NSAbs) against various antigens cause autoimmune encephalitis. Some of these antigens are also involved in the pathology of depression and anxiety. To study whether NSAbs are more common in plasma of individuals with depression and anxiety than in controls, and to investigate if NSAbs correlate with disease status, plasma samples of 819 individuals with a current diagnosis of depression and/or anxiety, 920 in remission and 492 individuals without these disorders were included in this study. Samples were tested by a combination of immunohistochemistry (IHC), staining on live rat hippocampus neurons and cell-based assay (CBA). By IHC, 50 (2.2%) samples showed immunoreactivity to rat brain tissue, with no significant differences between the aforementioned groups (22/819 vs 18/920 vs 11/492, P > 0.99). In addition, eight IHC positive samples were positive for NSAbs on live neurons (7/819 vs 0/920 vs 1/492, P = 0.006). The IHC-staining patterns of these eight samples were atypical for autoimmune encephalitis and accordingly, they tested negative for known NSAbs by CBA. No obvious difference in the clinical characteristics between individuals with or without NSAbs was observed. In conclusion, novel NSAbs were rare but predominately found in patients with current anxiety or depression indicating they might affect mental health in a small group of patients.info:eu-repo/semantics/publishedVersio

When Does Diversity Trump Ability (and Vice Versa) in Group Decision Making? A Simulation Study

It is often unclear which factor plays a more critical role in determining a group's performance: the diversity among members of the group or their individual abilities. In this study, we addressed this “diversity vs. ability” issue in a decision-making task. We conducted three simulation studies in which we manipulated agents' individual ability (or accuracy, in the context of our investigation) and group diversity by varying (1) the heuristics agents used to search task-relevant information (i.e., cues); (2) the size of their groups; (3) how much they had learned about a good cue search order; and (4) the magnitude of errors in the information they searched. In each study, we found that a manipulation reducing agents' individual accuracy simultaneously increased their group's diversity, leading to a conflict between the two. These conflicts enabled us to identify certain conditions under which diversity trumps individual accuracy, and vice versa. Specifically, we found that individual accuracy is more important in task environments in which cues differ greatly in the quality of their information, and diversity matters more when such differences are relatively small. Changing the size of a group and the amount of learning by an agent had a limited impact on this general effect of task environment. Furthermore, we found that a group achieves its highest accuracy when there is an intermediate amount of errors in the cue information, regardless of the environment and the heuristic used, an effect that we believe has not been previously reported and warrants further investigation

Differential tumor-targeting abilities of three single-domain antibody formats

The large molecular size of antibody drugs is considered one major factor preventing them from becoming more efficient therapeutics. Variable regions of heavy chain antibodies (HCAbs), or single-domain antibodies (sdAbs), are ideal building blocks for smaller antibodies due to their molecular size and enhanced stability. In the search for better antibody formats for in vivo imaging and/or therapy of cancer, three types of sdAb-based molecules directed against epidermal growth factor receptor (EGFR) were constructed, characterized and tested. Eleven sdAbs were isolated from a phage display library constructed from the sdAb repertoire of a llama immunized with a variant of EGFR. A pentameric sdAb, or pentabody, V2C-EG2 was constructed by fusing one of the sdAbs, EG2, to a pentamerization protein domain. A chimeric HCAb (cHCAb), EG2-hFc, was constructed by fusing EG2 to the fragment crystallizable (Fc) of human IgG1. Whereas EG2 and V2C-EG2 localized mainly in the kidneys after i.v. injection, EG2-hFc exhibited excellent tumor accumulation, and this was largely attributed to its long serum half life, which is comparable to that of IgGs. The moderate size (~80 kDa) and intact human Fc make HCAbs a unique antibody format which may outperform whole IgGs as imaging and therapeutic reagents.Peer reviewed: YesNRC publication: Ye