Evaluating performance in three-dimensional fluorescence microscopy

In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including techniques such as 3-D deconvolution and confocal. There has been a great deal of interest and some confusion about which of these methods is ‘better’, in principle or in practice. The motivation for the experiments reported here is to establish some rough guidelines for choosing the most appropriate method of microscopy for a given biological specimen. The approach is to compare the efficiency of photon collection, the image contrast and the signal-to-noise ratio achieved by the different methods at equivalent illumination, using a specimen in which the amount of out of focus background is adjustable over the range encountered with biological samples. We compared spot scanning confocal, spinning disk confocal and wide-field/deconvolution (WFD) microscopes and find that the ratio of out of focus background to in-focus signal can be used to predict which method of microscopy will provide the most useful image. We also find that the precision of measurements of net fluorescence yield is very much lower than expected for all modes of microscopy. Our analysis enabled a clear, quantitative delineation of the appropriate use of different imaging modes relative to the ratio of out-of-focus background to in-focus signal, and defines an upper limit to the useful range of the three most common modes of imaging

On different actions for the vacuum of bosonic string field theory

We study a family of kinetic operators in string field theory describing the theory around the closed string vacuum. Those operators are based on the analytical classical solutions of Takahashi and Tanimoto and are analogous to the pure ghost action usually referred to as "vacuum string field theory," but are much more general, and less singular than the pure ghost operator. The closed string vacuum is related to the D-brane vacuum by large, singular, gauge transformations or field redefinition, and all those different representations are related to each other by small gauge transformations. We try to clarify the nature of this singular gauge transformation. We also show that by choosing the Siegel gauge one recovers the propagator proposed in hep-th/0207266 that generates closed string surfaces.Comment: 15 page

Conformal symmetry, anomaly and effective action for metric-scalar gravity with torsion

We consider some aspects of conformal symmetry in a metric-scalar-torsion system. It is shown that, for some special choice of the action, torsion acts as a compensating field and the full theory is conformally equivalent to General Relativity on classical level. Due to the introduction of torsion, this equivalence can be provided for the positively-defined gravitational and scalar actions. One-loop divergences arising from the scalar loop are calculated and both the consequent anomaly and the anomaly-induced effective action are derived.Comment: 15 pages, LaTeX. Essentially extended version, in particular the discussion of the stability of the conformal factor in gravity theory with torsion and scalar added. Title also modified. To be published in Physics Letters

Closed String Amplitudes from Gauge Fixed String Field Theory

Closed string diagrams are derived from cubic open string field theory using a gauge fixed kinetic operator. The basic idea is to use a string propagator that does not generate a boundary to the world sheet. Using this propagator and the closed string vertex, the moduli space of closed string surfaces is covered, so closed string scattering amplitudes should be reproduced. This kinetic operator could be a gauge fixed form of the string field theory action around the closed string vacuum.Comment: 10 pages, revtex, 3 figures. Discussion on the covering of moduli expanded, version to appear in PR

Male frequent attenders of general practice and their help seeking preferences

Background: Low rates of health service usage by men are commonly linked to masculine values and traditional male gender roles. However, not all men conform to these stereotypical notions of masculinity, with some men choosing to attend health services on a frequent basis, for a variety of different reasons. This study draws upon the accounts of male frequent attenders of the General Practitioner's (GP) surgery, examining their help-seeking preferences and their reasons for choosing services within general practice over other sources of support. Methods: The study extends thematic analysis of interview data from the Self Care in Primary Care study (SCinPC), a large scale multi-method evaluation study of a self care programme delivered to frequent attenders of general practice. Data were collected from 34 semi-structured interviews conducted with men prior to their exposure to the intervention. Results: The ages of interviewed men ranged from 16 to 72 years, and 91% of the sample (n= 31) stated that they had a current health condition. The thematic analysis exposed diverse perspectives within male help-seeking preferences and the decision-making behind men's choice of services. The study also draws attention to the large variation in men's knowledge of available health services, particularly alternatives to general practice. Furthermore, the data revealed some men's lack of confidence in existing alternatives to general practice. Conclusions: The study highlights the complex nature of male help-seeking preferences, and provides evidence that there should be no 'one size fits all' approach to male service provision. It also provides impetus for conducting further studies into this under researched area of interest. © 2011 WPMH GmbH

Torsion as electromagnetism and spin

We show that it is possible to formulate the classical Einstein-Maxwell-Dirac theory of spinors interacting with the gravitational and electromagnetic fields as the Einstein-Cartan-Kibble-Sciama theory with the Ricci scalar of the traceless torsion, describing gravity, and the torsion trace acting as the electromagnetic potential.Comment: 6 pages; published versio

Robot Egomotion from the Deformation of Active Contours

Traditional sources of information for image-based computer vision algorithms have been points, lines, corners, and recently SIFT features (Lowe, 2004), which seem to represent at present the state of the art in feature definition. Alternatively, the present work explores the possibility of using tracked contours as informative features, especially in applications no

On zero sets in the Dirichlet space

We study the zeros sets of functions in the Dirichlet space. Using Carleson formula for Dirichlet integral, we obtain some new families of zero sets. We also show that any closed subset of E \subset \TT with logarithmic capacity zero is the accumulation points of the zeros of a function in the Dirichlet space. The zeros satisfy a growth restriction which depends on $E$.Comment: Journal of Geometric Analysis (2011

Lowest Order Constrained Variational Calculation of Structure Properties of Protoneutron Star

We calculate the structure properties of protoneutron star such as equation of state, maximum mass, radius and temperature profile using the lowest order constrained variational method. We show that the mass and radius of protoneutron star decrease by decreasing both entropy and temperature. For the protoneutron star, it is shown that the temperature is nearly constant in the core and drops rapidly near the crust.Comment: 14 pages, 12 figures. Int. J. Theor. Phys. (2008) in pres

Absorption Line Studies in the Halo

Significant progress has been made over the last few years to explore the gaseous halo of the Milky Way by way of absorption spectroscopy. I review recent results on absorption line studies in the halo using various instruments, such as the Far Ultraviolet Spectroscopic Explorer, the Space Telescope Imaging Spectrograph, and others. The new studies imply that the infall of low-metallicity gas, the interaction with the Magellanic Clouds, and the Galactic Fountain are responsible for the phenomenon of the intermediate- and high-velocity clouds in the halo. New measurements of highly-ionized gas in the vicinity of the Milky Way indicate that these clouds are embedded in a corona of hot gas that extends deep into the intergalactic space.Comment: 7 pages, 1 figure; Invited review at the conference "How does the Galaxy work ?", Granada/Spain, June 200