Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment

Lignin utilization during biomass conversion has been a major challenge for lignocellulosic biofuel. In particular, the conversion of lignin along with carbohydrate for fungible fuels and chemicals will both improve the overall carbon efficiency and reduce the need for chemical pretreatments. However, few biomass-converting microorganisms have the capacity to degrade all cell wall components including lignin, cellulose, and hemicellulose. We hereby evaluated a unique oleaginous fungus strain, Cunninghamella echinulata FR3, for its capacity to degrade lignin during biomass conversion to lipid, and the potential to carry out consolidated fermentation without chemical pretreatment, especially when combined with sorghum (Sorghum bicolor) bmr mutants with reduced lignin content. The study clearly showed that lignin was consumed together with carbohydrate during biomass conversion for all sorghum samples, which indicates that this organism has the potential for biomass conversion without chemical pretreatment. Even though dilute acid pretreatment of biomass resulted in more weight loss during fungal fermentation than untreated biomass, the lipid yields were comparable for untreated bmr6/bmr12 double mutant and dilute acid-pretreated wild-type biomass samples. The mechanisms for lignin degradation in oleaginous fungi were further elucidated through transcriptomics and chemical analysis. The studies showed that in C. echinulata FR3, the Fenton reaction may play an important role in lignin degradation. This discovery is among the first to show that a mechanism for lignin degradation similar to those found in white and brown rot basidiomycetous fungi exists in an oleaginous fungus. This study suggests that oleaginous fungi such as C. echinulata FR3 can be employed for complete biomass utilization in a consolidated platform without chemical pretreatment or can be used to convert lignin waste into lipids

Experimental study and application of similar materials in thick coal seam mining

The orthogonal experiments of similar materials were optimized and analyzed in order to accurately simulate the mechanical properties and the fracture evolution law of thick coal seam overlying strata during mining in this study. The experimental results indicated that similar materials using gypsum and calcium carbonate as cementing agents had a wide range of compressive strength (173.80 kPa–425.95 kPa) and were suitable for simulating various rock properties. Adding an appropriate amount of calcium carbonate can improve the brittleness and mechanical properties of similar materials using gypsum as cementing agents. The failure mode of similar materials transitioned from shear to tensile failure with the increase of the mass ratio of aggregate to cementing agents and the mass ratio of calcium carbonate to gypsum. Moreover, the compressive strength of similar materials rapidly decreased with an increase in the mass ratio of aggregate to cementing materials. And the compressive strength showed an increasing trend with the decrease of the mass ratio of calcium carbonate to gypsum. Moisture content had a significant impact on the density of similar materials, other parameters had small impacts. A thick coal seam mining experimental model was designed based on the experimental results of similar materials, which showed that Qianjiaying Mine may experience basic roof collapse when mining reached 58.1 m. The recommended periodic weighting pace for face pressure on the working face was approximately 15.0 m. Appropriate measures are necessary to adopt to prevent disasters after mining to a length of 58.1 m, followed by every 15.0 m of mining

Backscattering Echo Intensity Characteristics of Laser in Soil Explosion Dust

Soil dust generated by explosions can lead to the absorption and scattering of lasers, resulting in low detection and recognition accuracy for laser-based devices. Field tests to assess laser transmission characteristics in soil explosion dust are dangerous and involve uncontrollable environmental conditions. Instead, we propose using high-speed cameras and an indoor explosion chamber to assess the backscattering echo intensity characteristics of lasers in dust generated by small-scale explosive blasts in soil. We analyzed the influence of the mass of the explosive, depth of burial, and soil moisture content on crater features and temporal and spatial distributions of soil explosion dust. We also measured the backscattering echo intensity of a 905 nm laser at different heights. The results showed that the concentration of soil explosion dust was highest in the first 500 ms. The minimum normalized peak echo voltage ranged from 0.318 to 0.658. The backscattering echo intensity of the laser was found to be strongly correlated with the mean gray value of the monochrome image of soil explosion dust. This study provides experimental data and a theoretical basis for the accurate detection and recognition of lasers in soil explosion dust environments

Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment

Lignin utilization during biomass conversion has been a major challenge for lignocellulosic biofuel. In particular, the conversion of lignin along with carbohydrate for fungible fuels and chemicals will both improve the overall carbon efficiency and reduce the need for chemical pretreatments. However, few biomass-converting microorganisms have the capacity to degrade all cell wall components including lignin, cellulose, and hemicellulose. We hereby evaluated a unique oleaginous fungus strain, Cunninghamella echinulata FR3, for its capacity to degrade lignin during biomass conversion to lipid, and the potential to carry out consolidated fermentation without chemical pretreatment, especially when combined with sorghum (Sorghum bicolor) bmr mutants with reduced lignin content. The study clearly showed that lignin was consumed together with carbohydrate during biomass conversion for all sorghum samples, which indicates that this organism has the potential for biomass conversion without chemical pretreatment. Even though dilute acid pretreatment of biomass resulted in more weight loss during fungal fermentation than untreated biomass, the lipid yields were comparable for untreated bmr6/bmr12 double mutant and dilute acid-pretreated wild-type biomass samples. The mechanisms for lignin degradation in oleaginous fungi were further elucidated through transcriptomics and chemical analysis. The studies showed that in C. echinulata FR3, the Fenton reaction may play an important role in lignin degradation. This discovery is among the first to show that a mechanism for lignin degradation similar to those found in white and brown rot basidiomycetous fungi exists in an oleaginous fungus. This study suggests that oleaginous fungi such as C. echinulata FR3 can be employed for complete biomass utilization in a consolidated platform without chemical pretreatment or can be used to convert lignin waste into lipids

An integrated inversion framework for heterogeneous aquifer structure identification with single-sample generative adversarial network

Versión aceptada de https://doi.org/10.1016/j.jhydrol.2022.127844[Abstract:] Generating reasonable heterogeneous aquifer structures is essential for understanding the physicochemical processes controlling groundwater flow and solute transport better. The inversion process of aquifer structure identification is usually time-consuming. This study develops an integrated inversion framework, which combines the geological single-sample generative adversarial network (GeoSinGAN), the deep octave convolution dense residual network (DOCRN), and the iterative local updating ensemble smoother (ILUES), named GeoSinGAN-DOCRN-ILUES, for more efficiently generating heterogeneous aquifer structures. The performance of the integrated framework is illustrated by two synthetic contaminant experiments. We show that GeoSinGAN can generate heterogeneous aquifer structures with geostatistical characteristics similar to those of the training sample, while its training time is at least 10 times faster than that of typical approaches (e.g., multi-sample-based GAN). The octave convolution layer and multi-residual connection enable the DOCRN to map the heterogeneity structures to the state variable fields (e.g., hydraulic head, concentration distributions) while reducing the computational cost. The results show that the integrated inversion framework of GeoSinGAN and DOCRN can effectively and reasonably generate the heterogeneous aquifer structures.This work was funded by the National Key R&D Program of China (No.2018YFC1800904), the National Natural Science Foundation of China [NSFC: 41772253, 41972249], Jilin University through an innovation project awarded to the corresponding author [45119031A035], JLU Science and Technology Innovative Research Team [JLUSTIRT 2019TD-35] and partially supported by the Graduate Innovation Fund of Jilin University awarded to the first author (101832020CX233). Additional funding was provided by the Project (No. QQHR-2016-06) of Groundwater Quality Evaluation in Central City of Tsitsihar, Heilongjiang Province, China. We thank the ILUES and ConSinGAN developers for sharing their codes (https://github.com/cics-nd/cnn-inversion; https://github.com/tohinz/ConSinGAN). The geologic data used to represent permeability map distribution can be found in http://www.trainingimages.org. The authors would finally like to thank the two anonymous reviewers and the Editors for their constructive comments to improve the paper.China. National Key R&D Program of China; 2018YFC1800904China. National Natural Science Foundation of China; 41772253China. National Natural Science Foundation of China; 41972249China. Jilin University; 45119031A035China. JLU Science and Technology Innovative Research Team; JLUSTIRT 2019TD-35China. Graduate Innovation Fund of Jilin University; 101832020CX233China. Groundwater Quality Evaluation in Central City of Tsitsihar, Heilongjiang Province; QQHR-2016-0

Simultaneous Conversion of All Cell Wall Components by Oleaginous Fungus without Chemi-physical Pretreatment

Lignin utilization during biomass conversion has been a major challenge for lignocellulosic biofuel. In particular, the conversion of lignin along with carbohydrate for fungible fuels and chemicals will both improve the overall carbon efficiency and reduce the need for chemical pretreatments. However, few biomass-converting microorganisms have the capacity to degrade all cell wall components including lignin, cellulose, and hemicellulose. We hereby evaluated a unique oleaginous fungus strain, Cunninghamella echinulata FR3, for its capacity to degrade lignin during biomass conversion to lipid, and the potential to carry out consolidated fermentation without chemical pretreatment, especially when combined with sorghum (Sorghum bicolor) bmr mutants with reduced lignin content. The study clearly showed that lignin was consumed together with carbohydrate during biomass conversion for all sorghum samples, which indicates that this organism has the potential for biomass conversion without chemical pretreatment. Even though dilute acid pretreatment of biomass resulted in more weight loss during fungal fermentation than untreated biomass, the lipid yields were comparable for untreated bmr6/bmr12 double mutant and dilute acid-pretreated wild-type biomass samples. The mechanisms for lignin degradation in oleaginous fungi were further elucidated through transcriptomics and chemical analysis. The studies showed that in C. echinulata FR3, the Fenton reaction may play an important role in lignin degradation. This discovery is among the first to show that a mechanism for lignin degradation similar to those found in white and brown rot basidiomycetous fungi exists in an oleaginous fungus. This study suggests that oleaginous fungi such as C. echinulata FR3 can be employed for complete biomass utilization in a consolidated platform without chemical pretreatment or can be used to convert lignin waste into lipids