1,015 research outputs found
The arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis
Cell water permeability and cell wall properties are critical to survival of plant cells during freezing, however the underlying molecular mechanisms remain elusive. Here, we report that a specifically cold-induced nuclear protein, Tolerant to Chilling and Freezing 1 (TCF1), interacts with histones H3 and H4 and associates with chromatin containing a target gene, BLUE-COPPER-BINDING PROTEIN (BCB), encoding a glycosylphosphatidylinositol-anchored protein that regulates lignin biosynthesis. Loss of TCF1 function leads to reduced BCB transcription through affecting H3K4me2 and H3K27me3 levels within the BCB gene, resulting in reduced lignin content and enhanced freezing tolerance. Furthermore, plants with knocked-down BCB expression (amiRNA-BCB) under cold acclimation had reduced lignin accumulation and increased freezing tolerance. The pal1pal2 double mutant (lignin content reduced by 30% compared with WT) also showed the freezing tolerant phenotype, and TCF1 and BCB act upstream of PALs to regulate lignin content. In addition, TCF1 acts independently of the CBF (C-repeat binding factor) pathway. Our findings delineate a novel molecular pathway linking the TCF1-mediated cold-specific transcriptional program to lignin biosynthesis, thus achieving cell wall remodeling with increased freezing tolerance
Does Osmotic Stress Affect Natural Product Expression in Fungi?
Acknowledgments: Russell Kerr acknowledges the assistance of Nadia Prigoda-Lee, Marius Grote, Kate McQuillan and Stephanie Duffy, and generous financial support from NSERC, the Canada Research Chair program, the Jeanne and Jean-Louis Lévesque Foundation and the Atlantic Canada Opportunities Agency. Ka-Lai Pang thanks the president of National Taiwan Ocean University, Ching-Fong Chang, for a special fund to attend the workshop held in Charlottetown, Canada in 2014 where this work was discussed. Rob Capon and Zhuo Shang acknowledge support from the University of Queensland, and the UQ Institute for Molecular Bioscience. Zhuo Shang acknowledges the provision of an International Postgraduate Research Scholarship (IPRS) and a Centennial Scholarship by the University of Queensland. Catherine Roullier acknowledges the assistance of Marie-Claude Boumard and Thibaut Robiou du Pont, and support from Region Pays de la Loire, FrancePeer reviewedPublisher PD
The cardiac-restricted protein ADP-ribosylhydrolase-like 1 is essential for heart chamber outgrowth and acts on muscle actin filament assembly
AbstractAdprhl1, a member of the ADP-ribosylhydrolase protein family, is expressed exclusively in the developing heart of all vertebrates. In the amphibian Xenopus laevis, distribution of its mRNA is biased towards actively growing chamber myocardium. Morpholino oligonucleotide-mediated knockdown of all Adprhl1 variants inhibits striated myofibril assembly and prevents outgrowth of the ventricle. The resulting ventricles retain normal electrical conduction and express markers of chamber muscle differentiation but are functionally inert. Using a cardiac-specific Gal4 binary expression system, we show that the abundance of Adprhl1 protein in tadpole hearts is tightly controlled through a negative regulatory mechanism targeting the 5′-coding sequence of Xenopus adprhl1. Over-expression of full length (40kDa) Adprhl1 variants modified to escape such repression, also disrupts cardiac myofibrillogenesis. Disarrayed myofibrils persist that show extensive branching, with sarcomere division occurring at the actin-Z-disc boundary. Ultimately, Adprhl1-positive cells contain thin actin threads, connected to numerous circular branch points. Recombinant Adprhl1 can localize to stripes adjacent to the Z-disc, suggesting a direct role for Adprhl1 in modifying Z-disc and actin dynamics as heart chambers grow. Modelling the structure of Adprhl1 suggests this cardiac-specific protein is a pseudoenzyme, lacking key residues necessary for ADP-ribosylhydrolase catalytic activity
Warm Molecular Hydrogen Emission in Normal Edge-On Galaxies NGC 4565 and NGC 5907
We have observed warm molecular hydrogen in two nearby edge-on disk galaxies,
NGC 4565 and NGC 5907, using the Spitzer high-resolution infrared spectrograph.
The 0-0 S(0) 28.2 micron and 0-0 S(1) 17.0 micron pure rotational lines were
detected out to 10 kpc from the center of each galaxy on both sides of the
major axis, and in NGC 4565 the S(0) line was detected at r = 15 kpc on one
side. This location lies beyond a steep drop in the radio continuum emission
from cosmic rays in the disk. Despite indications that star formation activity
decreases with radius, the H2 excitation temperature and the ratio of the H2
line and the far-IR luminosity surface densities, Sigma_L(H2}/Sigma_L(TIR},
change very little as a function of radius, even into the diffuse outer region
of the disk of NGC 4565. This suggests that the source of excitation of the H2
operates over a large range of radii, and is broadly independent of the
strength and relative location of UV emission from young stars. Although
excitation in photodissociation regions is the most common explanation for the
widespread H2 emission, cosmic ray heating or shocks cannot be ruled out. The
inferred mass surface densities of warm molecular hydrogen in both edge-on
galaxies differ substantially, being 4(-60) M_solar/pc^2 and 3(-50)
M_solar/pc^2 at r = 10 kpc for NGC 4565 and NGC 5907, respectively. The higher
values represent very unlikely point-source upper limits. The point source case
is not supported by the observed emission distribution in the spectral slits.
These mass surface densities cannot support the observed rotation velocities in
excess of 200 km/s. Therefore, warm molecular hydrogen cannot account for dark
matter in these disk galaxies, contrary to what was implied by a previous ISO
study of the nearby edge-on galaxy NGC 891.Comment: Accepted for publication in the Astronomical Journal (20 pages, 17
figures, 7 tables
On the variability of quasars: a link between Eddington ratio and optical variability?
Repeat scans by the Sloan Digital Sky Survey (SDSS) of a 278 square degree
stripe along the Celestial equator have yielded an average of over 10
observations each for nearly 8,000 spectroscopically confirmed quasars. Over
2500 of these quasars are in the redshift range such that the CIV emission line
is visible in the SDSS spectrum. Utilising the width of these CIV lines and the
luminosity of the nearby continuum, we estimate black hole masses for these
objects. In an effort to isolate the effects of black hole mass and luminosity
on the photometric variability of our dataset, we create several subsamples by
binning in these two physical parameters. By comparing the ensemble structure
functions of the quasars in these bins, we are able to reproduce the well-known
anticorrelation between luminosity and variability, now showing that this
anticorrelation is independent of the black hole mass. In addition, we find a
correlation between variability and the mass of the central black hole. By
combining these two relations, we identify the Eddington ratio as a possible
driver of quasar variability, most likely due to differences in accretion
efficiency.Comment: 13 pages, 5 figures, Accepted for publication in MNRA
Developing an Australian Melanoma Clinical Outcomes Registry (MelCOR): a protocol paper
Introduction Australia has the highest incidence of melanoma in the world with variable care provided by a diverse range of clinicians. Clinical quality registries aim to identify these variations in care and provide anonymised, benchmarked feedback to clinicians and institutions to improve patient outcomes. The Australian Melanoma Clinical Outcomes Registry (MelCOR) aims to collect population-wide, clinical-level data for the early management of cutaneous melanoma and provide anonymised feedback to healthcare providers.
Methods and analysis A modified Delphi process will be undertaken to identify key clinical quality indicators for inclusion in the MelCOR pilot. MelCOR will prospectively collect data relevant to these quality indicators, initially for all people over the age of 18 years living in Victoria and Queensland with a melanoma diagnosis confirmed by histopathology, via a two-stage recruitment and consent process. In stage 1, existing State-based cancer registries contact the treating clinician and provide an opportunity for them to opt themselves or their patients out of direct contact with MelCOR. After stage 1, re-identifiable clinical data are provided to the MelCOR under a waiver of consent. In stage 2, the State-based cancer registry will approach the patient directly and invite them to opt in to MelCOR and share identifiable data. If a patient elects to opt in, MelCOR will be able to contact patients directly to collect patient-reported outcome measures. Aggregated data will be used to provide benchmarked, comparative feedback to participating institutions/clinicians.
Ethics and dissemination Following the successful collection of pilot data, the feasibility of an Australia-wide roll out will be evaluated. Key quality indicator data will be the core of the MelCOR dataset, with additional data points added later. Annual reports will be issued, first to the relevant stakeholders followed by the public. MelCOR is approved by the Alfred Ethics Committee (58280/127/20)
A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits
Melon is an economically important fruit crop that has been cultivated for thousands of years; however, the genetic basis and
history of its domestication still remain largely unknown. Here we report a comprehensive map of the genomic variation in
melon derived from the resequencing of 1,175 accessions, which represent the global diversity of the species. Our results suggest that three independent domestication events occurred in melon, two in India and one in Africa. We detected two independent sets of domestication sweeps, resulting in diverse characteristics of the two subspecies melo and agrestis during melon
breeding. Genome-wide association studies for 16 agronomic traits identified 208 loci significantly associated with fruit mass,
quality and morphological characters. This study sheds light on the domestication history of melon and provides a valuable
resource for genomics-assisted breeding of this important crop.info:eu-repo/semantics/acceptedVersio
A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits
Melon is an economically important fruit crop that has been cultivated for thousands of years; however, the genetic basis and history of its domestication still remain largely unknown. Here we report a comprehensive map of the genomic variation in melon derived from the resequencing of 1,175 accessions, which represent the global diversity of the species. Our results suggest that three independent domestication events occurred in melon, two in India and one in Africa. We detected two independent sets of domestication sweeps, resulting in diverse characteristics of the two subspecies melo and agrestis during melon breeding. Genome-wide association studies for 16 agronomic traits identified 208 loci significantly associated with fruit mass, quality and morphological characters. This study sheds light on the domestication history of melon and provides a valuable resource for genomics-assisted breeding of this important crop.This work was supported by funding from the Agricultural Science and Technology Innovation Program (to Yongyang Xu, S.H., Z.Z. and H.W.), the China Agriculture Research System (CARS-25 to Yongyang Xu and H.W.), the Leading Talents of Guangdong Province Program (00201515 to S.H.), the Shenzhen Municipal (The Peacock Plan KQTD2016113010482651 to S.H.), the Dapeng district government, National Natural Science Foundation of China (31772304 to Z.Z.), the Science and Technology Program of Guangdong (2018B020202007 to S.H.), the National Natural Science Foundation of China (31530066 to S.H.), the National Key R&D Program of China (2016YFD0101007 to S.H.), USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (2015-51181-24285 to Z.F.), the European Research Council (ERC-SEXYPARTH to A.B.), the Spanish Ministry of Economy and Competitiveness (AGL2015–64625-C2-1-R to J.G.-M.), Severo Ochoa Programme for Centres of Excellence in R&D 2016–2010 (SEV-2015–0533 to J.G.-M.), the CERCA Programme/Generalitat de Catalunya to J.G.-M. and the German Science Foundation (SPP1991 Taxon-OMICS to H.S.)
Extensive and drastically different alpine lake changes on Asia's high plateaus during the past four decades
Asia's high plateaus are sensitive to climate change and have been experiencing rapid warming over the past few decades. We found 99 new lakes and extensive lake expansion on the Tibetan Plateau during the last four decades, 1970–2013, due to increased precipitation and cryospheric contributions to its water balance. This contrasts with disappearing lakes and drastic shrinkage of lake areas on the adjacent Mongolian Plateau: 208 lakes disappeared, and 75% of the remaining lakes have shrunk. We detected a statistically significant coincidental timing of lake area changes in both plateaus, associated with the climate regime shift that occurred during 1997/1998. This distinct change in 1997/1998 is thought to be driven by large-scale atmospheric circulation changes in response to climate warming. Our findings reveal that these two adjacent plateaus have been changing in opposite directions in response to climate change. These findings shed light on the complex role of the regional climate and water cycles and provide useful information for ecological and water resource planning in these fragile landscapes.Publisher PDFPeer reviewe
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