Évaluation des performances de l’automate STA R Max® (Stago) pour les paramètres d’hémostase de routineEvaluation of the coagulometer STA R Max (R) (Stago) for routine coagulation parameters

International audienceLe STA R Max® (Stago) est un coagulomètre à détection viscosimétrique. Il est doté d’un logiciel innovant (le système STA Coag Expert®) comportant un menu « accréditation » et permet une traçabilité totale de 5 ans. Le but de cette étude était d’évaluer ses performances avec les 3 méthodes de détection embarquées en vue d’une vérification de méthode selon la norme ISO 15189. Ce travail s’est déroulé au sein du service d’hématologie biologique du CHU de Clermont-Ferrand qui possède 4 STA R Max® répartis sur 2 sites et dédiés à l’activité de routine d’hémostase. Les paramètres étudiés étaient les suivants : le taux de prothrombine (TP), les temps de céphaline avec activateurs (TCA et TCK), le fibrinogène, l’activité anti-Xa et les D-dimères ; chacun testé dans les zones normales et pathologiques. Pour les analyses TP, TCA, TCK et fibrinogène, les coefficients de variation (CV) des répétabilités étaient inférieurs à 4,0 %. Les CV de répétabilité de l’activité anti-Xa et des D-dimères étaient respectivement au maximum de 4,0 % et 7,9 %. Les CV de la fidélité intermédiaire étaient inférieurs à 5,0 % pour TP, TCA, TCK et fibrinogène, 14,9 % pour l’activité anti-Xa et 8,6 % pour les D-dimères. Le biais (évaluant l’inexactitude) était inférieur à 8,7 % pour le TP, TCA et TCK, à 5,0 % pour le fibrinogène et inférieur à 15,5 % pour l’activité anti-Xa. L’étude de la concordance entre les 4 automates (corrélation intra-classe) retrouvait des coefficients de corrélation intra-classe de 0,99 pour tous les paramètres sauf le TCA (0,98). La concordance entre les automates était excellente. L’ensemble des résultats obtenus sont satisfaisants au regard des recommandations du Groupe français d’études sur l’hémostase et la thrombose (GFHT) et le fournisseur. Le STA R Max® est un analyseur performant pour gérer l’activité d’hémostase de routine d’un laboratoire et présente l’avantage de faciliter la démarche d’accréditation

Management of severe hypercalcaemia: results of the French-speaking physicians’ practice survey

International audienc

Lysine methylation of non-histone proteins is involved in the response of Arabidopsis plants to a stress induced by cadmium

info:eu-repo/semantics/nonPublishe

FC030: Diagnostic Yield of Exome Sequencing in Hypertensive Nephropathy

BACKGROUND AND AIMS : Hypertensive nephrosclerosis ranks as one of the most frequent causes of chronic kidney disease (CKD) worldwide and is deemed to be especially prevalent among patients of African ancestry [1]. The very existence of hypertensive nephropathy has been called into question, especially in young adults. Its diagnostic framework is based on non-specific clinical criteria, and its histopathological features are in fact unspecific. [...

Production and stability study of a hospital parenteral nutrition solution for neonates

Standard parenteral nutrition solutions are mixtures comprising interacting components that may degrade themselves over time. The objective of this study was to investigate the physicochemical and microbiological stability of a hospital preparation for parenteral nutrition in neonatology. The analyses were performed throughout the storage of the preparations at 2–8 °C (up to 4 months). The extent of stability was based on the determination of amino acids dosage, visual and physicochemical properties (glucose and electrolytes concentrations, pH and osmolality measurements, particle counting) and microbiological analysis (sterility test). A thermal degradation of ascorbic acid was conducted to evaluate the antioxidant properties of the parenteral mixture. Physicochemical and microbiological controls were found to comply with the specifications. Amino acids showed a good stability throughout the 4months storage except for cysteine, which was progressively degraded to cystine, conferring a yellow coloration to parenteral solutions. Parenteral nutrition standards solutions remain stable for 4 months at 2–8 °C, ensuring safe administration in preterm infants. Keywords: Stability-indicating method study, Parenteral nutrition, Neonatology, Amino acids, High-performance liquid chromatographi

Common bean resistance to Xanthomonas is associated with upregulation of the salicylic acid pathway and downregulation of photosynthesis

International audienceackground Common bacterial blight (CBB) caused byXanthomonas phaseolipv.phaseoliandXanthomonas citripv.fuscansis one of the major threats to common bean crops (Phaseolus vulgarisL.). Resistance to CBB is particularly complex as 26 quantitative resistance loci to CBB have been described so far. To date, transcriptomic studies after CBB infection have been very scarce and the molecular mechanisms underlying susceptibility or resistance are largely unknown. Results We sequenced and annotated the genomes of two common bean genotypes being either resistant (BAT93) or susceptible (JaloEEP558) to CBB. Reciprocal BLASTp analysis led to a list of 20,787 homologs between these genotypes and the common bean reference genome (G19833), which provides a solid dataset for further comparative analyses. RNA-Seq after inoculation withX. phaseolipv.phaseolishowed that the susceptible genotype initiated a more intense and diverse biological response than the resistant genotype. Resistance was linked to upregulation of the salicylic acid pathway and downregulation of photosynthesis and sugar metabolism, while susceptibility was linked to downregulation of resistance genes and upregulation of the ethylene pathway and of genes involved in cell wall modification. Conclusions This study helps better understanding the mechanisms occurring during the early colonization phase of common bean byXanthomonasand unveils new actors potentially important for resistance and susceptibility to CBB. We discuss the potential link between the pathways induced during bean colonization and genes induced by transcription activator-like effectors (TALEs), as illustrated in otherXanthomonaspathovars

Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress

International audienceThe mechanisms underlying the response and adaptation of plants to excess of trace elements are not fully described. Here, we analyzed the importance of protein lysine methylation for plants to cope with cadmium. We analyzed the effect of cadmium on lysine-methylated proteins and protein lysine methyltransferases (KMTs) in two cadmium-sensitive species, Arabidopsis thaliana and A. lyrata, and in three populations of A. halleri with contrasting cadmium accumulation and tolerance traits. We showed that some proteins are differentially methylated at lysine residues in response to Cd and that a few genes coding KMTs are regulated by cadmium. Also, we showed that nine out of 23 A. thaliana mutants disrupted in KMT genes have a tolerance to cadmium that is significantly different from that of wild-type seedlings. We further characterized two of these mutants, one was knocked-out in the calmodulin lysine methyltransferase gene and displayed increased tolerance to cadmium, the other was interrupted in a KMT gene of unknown function and showed a decreased capacity to cope with cadmium. Together, our results showed that lysine methylation of non-histone proteins is impacted by cadmium and that several methylation events are important for modulating the response of Arabidopsis plants to cadmium stress

Environmental transcriptomes of invasive dreissena, a model species in ecotoxicology and invasion biology

International audienceDreissenids are established model species for ecological and ecotoxicological studies, since they are sessile and filter feeder organisms and reflect in situ freshwater quality. Despite this strong interest for hydrosystem biomonitoring, omics data are still scarce. In the present study, we achieved full de novo assembly transcriptomes of digestive glands to gain insight into Dreissena polymorpha and D. rostriformis bugensis molecular knowledge. Transcriptomes were obtained by Illumina RNA sequencing of seventy-nine organisms issued from fifteen populations inhabiting sites that exhibits multiple freshwater contamination levels and different hydrosystem topographies (open or closed systems). Based on a recent de novo assembly algorithm, we carried out a complete, quality-checked and annotated transcriptomes. The power of the present study lies in the completeness of transcriptomes gathering multipopulational organisms sequencing and its full availability through an open access interface that gives a friendly and ready-to-use access to data. The use of such data for proteogenomic and targeted biological pathway investigations purpose is promising as they are first full transcriptomes for this two Dreissena species

Evidence for the involvement of protein lysine methylation in the response of sensitive and tolerant Arabidopsis species to cadmium stress

The mechanisms underlying the response and adaptation of plants to excess of trace elements are not fully described. Here, we analyzed the importance of protein lysine methylation for plants to cope with cadmium. We analyzed the effect of cadmium on lysine-methylated proteins and protein lysine methyltransferases (KMTs) in three Arabidopsis species. Arabidopsis thaliana and A. lyrata were used as cadmium-sensitive models and compared with three populations of A. halleri with different properties of accumulation and tolerance to cadmium. Immunoblotting, protein mass spectrometry, and gene expression analysis showed that the expression of some lysine-methylated proteins and genes coding KMTs is regulated by cadmium. Using a genetic screening, we showed that nine A. thaliana mutants interrupted in KMT genes have a tolerance to cadmium that is significantly different from that of wild-type seedlings. We further characterized two of these mutants, one was knocked-out in the calmodulin lysine methyltransferase gene and displayed increased tolerance to cadmium, the other was interrupted in a KMT gene of unknown function and showed a decreased capacity to cope with cadmium. Together, our results showed that the fine-tuned regulation of nonhistone proteins by lysine methylation has a role in the response of Arabidopsis plants to cadmium stress