Desarrollo de nuevas tecnologías en proteómica de segunda generación y su aplicación al estudio de cambios dinamicos relacionados con la disfuncion cardivascular

Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 17-10-200

Event-driven implementation of deep spiking convolutional neural networks for supervised classification using the SpiNNaker neuromorphic platform

Neural networks have enabled great advances in recent times due mainly to improved parallel computing capabilities in accordance to Moore’s Law, which allowed reducing the time needed for the parameter learning of complex, multi-layered neural architectures. However, with silicon technology reaching its physical limits, new types of computing paradigms are needed to increase the power efficiency of learning algorithms, especially for dealing with deep spatio-temporal knowledge on embedded applications. With the goal of mimicking the brain’s power efficiency, new hardware architectures such as the SpiNNaker board have been built. Furthermore, recent works have shown that networks using spiking neurons as learning units can match classical neural networks in supervised tasks. In this paper, we show that the implementation of state-of-the-art models on both the MNIST and the event-based NMNIST digit recognition datasets is possible on neuromorphic hardware. We use two approaches, by directly converting a classical neural network to its spiking version and by training a spiking network from scratch. For both cases, software simulations and implementations into a SpiNNaker 103 machine were performed. Numerical results approaching the state of the art on digit recognition are presented, and a new method to decrease the spike rate needed for the task is proposed, which allows a significant reduction of the spikes (up to 34 times for a fully connected architecture) while preserving the accuracy of the system. With this method, we provide new insights on the capabilities offered by networks of spiking neurons to efficiently encode spatio-temporal information.Consejo Nacional de Ciencia Y Tecnología (México) FC2016-1961European Union's Horizon 2020 No 824164 HERMESMinisterio de Ciencia, Innovación y Universidades TEC2015-63884-C2-1-

Intermolecular Carbonyl-olefin Metathesis with Vinyl Ethers Catalyzed by Homogeneous and Solid Acids in Flow

This is the peer reviewed version of the following article: M. Á. Rivero-Crespo, M. Tejeda-Serrano, H. Pérez-Sánchez, J. P. Cerón-Carrasco, A. Leyva-Pérez, Angew. Chem. Int. Ed. 2020, 59, 3846, which has been published in final form at https://doi.org/10.1002/anie.201909597. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.[EN] The carbonyl-olefin metathesis reaction has experienced significant advances in the last seven years with new catalysts and reaction protocols. However, most of these procedures involve soluble catalysts for intramolecular reactions in batch. Herein, we show that recoverable, inexpensive, easy to handle, non-toxic, and widely available simple solid acids, such as the aluminosilicate montmorillonite, can catalyze the intermolecular carbonyl-olefin metathesis of aromatic ketones and aldehydes with vinyl ethers in-flow, to give alkenes with complete trans stereoselectivity on multi-gram scale and high yields. Experimental and computational data support a mechanism based on a carbocation-induced Grob fragmentation. These results open the way for the industrial implementation of carbonyl-olefin metathesis over solid catalysts in continuous mode, which is still the origin and main application of the parent alkene-alkene cross-metathesis.Financial support by MINECO through the Severo Ochoa program (SEV-2016-0683), Excellence program (CTQ 2017-86735-P, CTQ 2017-87974-R), Retos Col. (RTC-2017-6331-5), and "Convocatoria 2014 de Ayudas Fundacion BBVA a Investigadores y Creadores Culturales" is acknowledged. M.A.R.-C. and M.T.-S. thank ITQ for the concession of a contract. This research was partially supported by the supercomputing infrastructure of Poznan Supercomputing Center.Rivero-Crespo, MÁ.; Tejeda-Serrano, M.; Perez-Sánchez, H.; Cerón-Carrasco, JP.; Leyva Perez, A. (2020). Intermolecular Carbonyl-olefin Metathesis with Vinyl Ethers Catalyzed by Homogeneous and Solid Acids in Flow. Angewandte Chemie International Edition. 59(10):3846-3849. https://doi.org/10.1002/anie.201909597S384638495910Becker, M. R., Watson, R. B., & Schindler, C. S. (2018). Beyond olefins: new metathesis directions for synthesis. Chemical Society Reviews, 47(21), 7867-7881. doi:10.1039/c8cs00391bGriffith, A. K., Vanos, C. M., & Lambert, T. H. (2012). Organocatalytic Carbonyl-Olefin Metathesis. Journal of the American Chemical Society, 134(45), 18581-18584. doi:10.1021/ja309650uLudwig, J. R., Zimmerman, P. M., Gianino, J. B., & Schindler, C. S. (2016). Iron(III)-catalysed carbonyl–olefin metathesis. Nature, 533(7603), 374-379. doi:10.1038/nature17432Ludwig, J. R., Phan, S., McAtee, C. C., Zimmerman, P. M., Devery, J. J., & Schindler, C. S. (2017). Mechanistic Investigations of the Iron(III)-Catalyzed Carbonyl-Olefin Metathesis Reaction. Journal of the American Chemical Society, 139(31), 10832-10842. doi:10.1021/jacs.7b05641For reviews on carbonyl olefin metathesis see:Schindler, C., & Ludwig, J. (2017). Lewis Acid Catalyzed Carbonyl–Olefin Metathesis. Synlett, 28(13), 1501-1509. doi:10.1055/s-0036-1588827T. H. Lambert Synlett2019 ahead of print.For examples of solid-catalyzed low-temperature alkene metathesis see:Mougel, V., Chan, K.-W., Siddiqi, G., Kawakita, K., Nagae, H., Tsurugi, H., … Copéret, C. (2016). Low Temperature Activation of Supported Metathesis Catalysts by Organosilicon Reducing Agents. ACS Central Science, 2(8), 569-576. doi:10.1021/acscentsci.6b00176Korzyński, M. D., Consoli, D. F., Zhang, S., Román-Leshkov, Y., & Dincă, M. (2018). Activation of Methyltrioxorhenium for Olefin Metathesis in a Zirconium-Based Metal–Organic Framework. Journal of the American Chemical Society, 140(22), 6956-6960. doi:10.1021/jacs.8b02837Van Schaik, H.-P., Vijn, R.-J., & Bickelhaupt, F. (1994). Acid-Catalyzed Olefination of Benzaldehyde. Angewandte Chemie International Edition in English, 33(1516), 1611-1612. doi:10.1002/anie.199416111Van Schaik, H.-P., Vijn, R.-J., & Bickelhaupt, F. (1994). Säurekatalysierte Olefinierung von Benzaldehyd. Angewandte Chemie, 106(15-16), 1703-1704. doi:10.1002/ange.19941061529For pure Bronsted acid-catalyzed reactions see:Ludwig, J. R., Watson, R. B., Nasrallah, D. J., Gianino, J. B., Zimmerman, P. M., Wiscons, R. A., & Schindler, C. S. (2018). Interrupted carbonyl-olefin metathesis via oxygen atom transfer. Science, 361(6409), 1363-1369. doi:10.1126/science.aar8238Catti, L., & Tiefenbacher, K. (2018). Brønsted Acid-Catalyzed Carbonyl-Olefin Metathesis inside a Self-Assembled Supramolecular Host. Angewandte Chemie International Edition, 57(44), 14589-14592. doi:10.1002/anie.201712141Catti, L., & Tiefenbacher, K. (2018). Brønsted-Säure-katalysierte Carbonyl-Olefin-Metathese in einer selbstorganisierten supramolekularen Wirtstruktur. Angewandte Chemie, 130(44), 14797-14800. doi:10.1002/ange.201712141For intermolecular reactions see:Ni, S., & Franzén, J. (2018). Carbocation catalysed ring closing aldehyde–olefin metathesis. Chemical Communications, 54(92), 12982-12985. doi:10.1039/c8cc06734aPitzer, L., Sandfort, F., Strieth‐Kalthoff, F., & Glorius, F. (2018). Carbonyl–Olefin Cross‐Metathesis Through a Visible‐Light‐Induced 1,3‐Diol Formation and Fragmentation Sequence. Angewandte Chemie International Edition, 57(49), 16219-16223. doi:10.1002/anie.201810221Pitzer, L., Sandfort, F., Strieth‐Kalthoff, F., & Glorius, F. (2018). Carbonyl‐Olefin‐Kreuzmetathese mittels Licht‐induzierter 1,3‐Diol‐Bildung‐ und Fragmentierungssequenz. Angewandte Chemie, 130(49), 16453-16457. doi:10.1002/ange.201810221Tran, U. P. N., Oss, G., Pace, D. P., Ho, J., & Nguyen, T. V. (2018). Tropylium-promoted carbonyl–olefin metathesis reactions. Chemical Science, 9(23), 5145-5151. doi:10.1039/c8sc00907dTran, U. P. N., Oss, G., Breugst, M., Detmar, E., Pace, D. P., Liyanto, K., & Nguyen, T. V. (2018). Carbonyl–Olefin Metathesis Catalyzed by Molecular Iodine. ACS Catalysis, 9(2), 912-919. doi:10.1021/acscatal.8b03769Lewis, J. D., Van de Vyver, S., & Román‐Leshkov, Y. (2015). Acid–Base Pairs in Lewis Acidic Zeolites Promote Direct Aldol Reactions by Soft Enolization. Angewandte Chemie International Edition, 54(34), 9835-9838. doi:10.1002/anie.201502939Lewis, J. D., Van de Vyver, S., & Román‐Leshkov, Y. (2015). Acid–Base Pairs in Lewis Acidic Zeolites Promote Direct Aldol Reactions by Soft Enolization. Angewandte Chemie, 127(34), 9973-9976. doi:10.1002/ange.201502939Fortea-Pérez, F. R., Mon, M., Ferrando-Soria, J., Boronat, M., Leyva-Pérez, A., Corma, A., … Pardo, E. (2017). The MOF-driven synthesis of supported palladium clusters with catalytic activity for carbene-mediated chemistry. Nature Materials, 16(7), 760-766. doi:10.1038/nmat4910Oliver-Meseguer, J., Boronat, M., Vidal-Moya, A., Concepción, P., Rivero-Crespo, M. Á., Leyva-Pérez, A., & Corma, A. (2018). Generation and Reactivity of Electron-Rich Carbenes on the Surface of Catalytic Gold Nanoparticles. Journal of the American Chemical Society, 140(9), 3215-3218. doi:10.1021/jacs.7b13696Rivero‐Crespo, M. A., Mon, M., Ferrando‐Soria, J., Lopes, C. W., Boronat, M., Leyva‐Pérez, A., … Pardo, E. (2018). Confined Pt 1 1+ Water Clusters in a MOF Catalyze the Low‐Temperature Water–Gas Shift Reaction with both CO 2 Oxygen Atoms Coming from Water. Angewandte Chemie International Edition, 57(52), 17094-17099. doi:10.1002/anie.201810251Rivero‐Crespo, M. A., Mon, M., Ferrando‐Soria, J., Lopes, C. W., Boronat, M., Leyva‐Pérez, A., … Pardo, E. (2018). Confined Pt 1 1+ Water Clusters in a MOF Catalyze the Low‐Temperature Water–Gas Shift Reaction with both CO 2 Oxygen Atoms Coming from Water. Angewandte Chemie, 130(52), 17340-17345. doi:10.1002/ange.201810251Tejeda-Serrano, M., Mon, M., Ross, B., Gonell, F., Ferrando-Soria, J., Corma, A., … Pardo, E. (2018). Isolated Fe(III)–O Sites Catalyze the Hydrogenation of Acetylene in Ethylene Flows under Front-End Industrial Conditions. Journal of the American Chemical Society, 140(28), 8827-8832. doi:10.1021/jacs.8b04669Ma, L., Li, W., Xi, H., Bai, X., Ma, E., Yan, X., & Li, Z. (2016). FeCl3 -Catalyzed Ring-Closing Carbonyl-Olefin Metathesis. Angewandte Chemie International Edition, 55(35), 10410-10413. doi:10.1002/anie.201604349Ma, L., Li, W., Xi, H., Bai, X., Ma, E., Yan, X., & Li, Z. (2016). FeCl3 -Catalyzed Ring-Closing Carbonyl-Olefin Metathesis. Angewandte Chemie, 128(35), 10566-10569. doi:10.1002/ange.201604349McAtee, C. C., Riehl, P. S., & Schindler, C. S. (2017). Polycyclic Aromatic Hydrocarbons via Iron(III)-Catalyzed Carbonyl–Olefin Metathesis. Journal of the American Chemical Society, 139(8), 2960-2963. doi:10.1021/jacs.7b01114Niyomchon, S., Oppedisano, A., Aillard, P., & Maulide, N. (2017). A three-membered ring approach to carbonyl olefination. Nature Communications, 8(1). doi:10.1038/s41467-017-01036-yWatson, R. B., & Schindler, C. S. (2017). Iron-Catalyzed Synthesis of Tetrahydronaphthalenes via 3,4-Dihydro-2H-pyran Intermediates. Organic Letters, 20(1), 68-71. doi:10.1021/acs.orglett.7b03367Groso, E. J., Golonka, A. N., Harding, R. A., Alexander, B. W., Sodano, T. M., & Schindler, C. S. (2018). 3-Aryl-2,5-Dihydropyrroles via Catalytic Carbonyl-Olefin Metathesis. ACS Catalysis, 8(3), 2006-2011. doi:10.1021/acscatal.7b03769Albright, H., Riehl, P. S., McAtee, C. C., Reid, J. P., Ludwig, J. R., Karp, L. A., … Schindler, C. S. (2018). Catalytic Carbonyl-Olefin Metathesis of Aliphatic Ketones: Iron(III) Homo-Dimers as Lewis Acidic Superelectrophiles. Journal of the American Chemical Society, 141(4), 1690-1700. doi:10.1021/jacs.8b11840Śliwa, M., Samson, K., Ruggiero–Mikołajczyk, M., Żelazny, A., & Grabowski, R. (2014). Influence of Montmorillonite K10 Modification with Tungstophosphoric Acid on Hybrid Catalyst Activity in Direct Dimethyl Ether Synthesis from Syngas. Catalysis Letters, 144(11), 1884-1893. doi:10.1007/s10562-014-1359-5Cabrero-Antonino, J. R., Leyva-Pérez, A., & Corma, A. (2015). Beyond Acid Strength in Zeolites: Soft Framework Counteranions for Stabilization of Carbocations on Zeolites and Its Implication in Organic Synthesis. Angewandte Chemie International Edition, 54(19), 5658-5661. doi:10.1002/anie.201500864Cabrero-Antonino, J. R., Leyva-Pérez, A., & Corma, A. (2015). Beyond Acid Strength in Zeolites: Soft Framework Counteranions for Stabilization of Carbocations on Zeolites and Its Implication in Organic Synthesis. Angewandte Chemie, 127(19), 5750-5753. doi:10.1002/ange.201500864Gassman, P. G., & Burns, S. J. (1988). General method for the synthesis of enol ethers (vinyl ethers) from acetals. The Journal of Organic Chemistry, 53(23), 5574-5576. doi:10.1021/jo00258a043Yamamoto, T., Eki, T., Nagumo, S., Suemune, H., & Sakai, K. (1992). Drastic ring transformation reactions of fused bicyclic rings to bridged bicyclic rings. Tetrahedron, 48(22), 4517-4524. doi:10.1016/s0040-4020(01)81224-2Nagumo, S., Matsukuma, A., Suemune, H., & Sakai, K. (1993). Novel ring cleavage based on intermolecular aldol condensation. Tetrahedron, 49(46), 10501-10510. doi:10.1016/s0040-4020(01)81545-3Suemune, H., Yoshida, O., Uchida, J., Nomura, Y., Tanaka, M., & Sakai, K. (1995). Asymmetric ring cleavage reaction based on crossed aldol condensation. 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METADOCK 2: a high-throughput parallel metaheuristic scheme for molecular docking

[EN] Motivation Molecular docking methods are extensively used to predict the interaction between protein-ligand systems in terms of structure and binding affinity, through the optimization of a physics-based scoring function. However, the computational requirements of these simulations grow exponentially with: (i) the global optimization procedure, (ii) the number and degrees of freedom of molecular conformations generated and (iii) the mathematical complexity of the scoring function. Results In this work, we introduce a novel molecular docking method named METADOCK 2, which incorporates several novel features, such as (i) a ligand-dependent blind docking approach that exhaustively scans the whole protein surface to detect novel allosteric sites, (ii) an optimization method to enable the use of a wide branch of metaheuristics and (iii) a heterogeneous implementation based on multicore CPUs and multiple graphics processing units. Two representative scoring functions implemented in METADOCK 2 are extensively evaluated in terms of computational performance and accuracy using several benchmarks (such as the well-known DUD) against AutoDock 4.2 and AutoDock Vina. Results place METADOCK 2 as an efficient and accurate docking methodology able to deal with complex systems where computational demands are staggering and which outperforms both AutoDock Vina and AutoDock 4.This work was partially supported by the Fundación Séneca del Centro de Coordinación de la Investigación de la Región de Murcia [Projects 20813/PI/ 18, 20988/PI/18, 20524/PDC/18] and by the Spanish Ministry of Science, Innovation and Universities [TIN2016-78799-P (AEI/FEDER, UE), CTQ2017-87974-R]. The authors thankfully acknowledge the computer resources at CTE-POWER and the technical support provided by Barcelona Supercomputing Center - Centro Nacional de Supercomputación [RES-BCV2018-3-0008].Imbernón, B.; Serrano, A.; Bueno-Crespo, A.; Abellán, JL.; Pérez-Sánchez, H.; Cecilia-Canales, JM. (2020). METADOCK 2: a high-throughput parallel metaheuristic scheme for molecular docking. Bioinformatics. 1-6. https://doi.org/10.1093/bioinformatics/btz958S16Bianchi, L., Dorigo, M., Gambardella, L. M., & Gutjahr, W. J. (2008). A survey on metaheuristics for stochastic combinatorial optimization. Natural Computing, 8(2), 239-287. doi:10.1007/s11047-008-9098-4Cecilia, J. M., Llanes, A., Abellán, J. L., Gómez-Luna, J., Chang, L.-W., & Hwu, W.-M. W. (2018). High-throughput Ant Colony Optimization on graphics processing units. Journal of Parallel and Distributed Computing, 113, 261-274. doi:10.1016/j.jpdc.2017.12.002Desiraju, G., & Steiner, T. (2001). 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LEADS-PEP: A Benchmark Data Set for Assessment of Peptide Docking Performance. Journal of Chemical Information and Modeling, 56(1), 188-200. doi:10.1021/acs.jcim.5b00234Llanes, A., Muñoz, A., Bueno-Crespo, A., García-Valverde, T., Sánchez, A., Arcas-Túnez, F., … M. Cecilia, J. (2016). Soft Computing Techniques for the Protein Folding Problem on High Performance Computing Architectures. Current Drug Targets, 17(14), 1626-1648. doi:10.2174/1389450117666160201114028McIntosh-Smith, S., Price, J., Sessions, R. B., & Ibarra, A. A. (2014). High performance in silico virtual drug screening on many-core processors. The International Journal of High Performance Computing Applications, 29(2), 119-134. doi:10.1177/1094342014528252Mehler, E. L., & Solmajer, T. (1991). Electrostatic effects in proteins: comparison of dielectric and charge models. «Protein Engineering, Design and Selection», 4(8), 903-910. doi:10.1093/protein/4.8.903Morris, G. M., Goodsell, D. S., Halliday, R. S., Huey, R., Hart, W. E., Belew, R. K., & Olson, A. J. (1998). Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function. Journal of Computational Chemistry, 19(14), 1639-1662. doi:10.1002/(sici)1096-987x(19981115)19:143.0.co;2-bMysinger, M. M., Carchia, M., Irwin, J. J., & Shoichet, B. K. (2012). Directory of Useful Decoys, Enhanced (DUD-E): Better Ligands and Decoys for Better Benchmarking. Journal of Medicinal Chemistry, 55(14), 6582-6594. doi:10.1021/jm300687eO’Boyle, N. M., Banck, M., James, C. A., Morley, C., Vandermeersch, T., & Hutchison, G. R. (2011). Open Babel: An open chemical toolbox. Journal of Cheminformatics, 3(1). doi:10.1186/1758-2946-3-33Sakurai, Y., Kolokoltsov, A. A., Chen, C.-C., Tidwell, M. W., Bauta, W. E., Klugbauer, N., … Davey, R. A. (2015). Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment. Science, 347(6225), 995-998. doi:10.1126/science.1258758Sánchez-Linares, I., Pérez-Sánchez, H., Cecilia, J. M., & García, J. M. (2012). High-Throughput parallel blind Virtual Screening using BINDSURF. BMC Bioinformatics, 13(S14). doi:10.1186/1471-2105-13-s14-s13Sliwoski, G., Kothiwale, S., Meiler, J., & Lowe, E. W. (2013). Computational Methods in Drug Discovery. Pharmacological Reviews, 66(1), 334-395. doi:10.1124/pr.112.007336Sörensen, K. (2013). Metaheuristics-the metaphor exposed. International Transactions in Operational Research, 22(1), 3-18. doi:10.1111/itor.12001Yuan, S., Chan, J. F.-W., den-Haan, H., Chik, K. K.-H., Zhang, A. J., Chan, C. C.-S., … Yuen, K.-Y. (2017). Structure-based discovery of clinically approved drugs as Zika virus NS2B-NS3 protease inhibitors that potently inhibit Zika virus infection in vitro and in vivo. Antiviral Research, 145, 33-43. doi:10.1016/j.antiviral.2017.07.00

Calibration of a high spatial resolution laser two-color heterodyne interferometer for density profile measurements in the TJ-II stellarator

Proceedings of: 17th Topical Conference on High-Temperature Plasma Diagnostics, Albuquerque, New Mexico, 11-15 May 2008A high spatial resolution two-color (CO2, lambda=10.6 mum, He-Ne, lambda=633 nm) interferometer for density profile measurements in the TJ-II stellarator is under development and installation, based in the currently operational single channel two-color heterodyne interferometer. To achieve the objectives of 32 channels, with 4-5 mm lateral separation between plasma chords, careful design and calibration of the interferometric waveforms for both the measurement and vibration compensation wavelengths are undertaken. The first step has been to set up in our laboratories an expanded-beam heterodyne/homodyne interferometer to evaluate the quality of both interferometric wavefronts, a reported source of poor vibration compensation and thus low resolution in the density profile measurements. This novel interferometric setup has allowed us to calibrate the spatial resolution in the profile measurements resulting in ~2 mm lateral resolution in the reconstruction of the interferometric wavefront

Application of a novel statistical model for quantitative proteomics by labeling to the study of VEGF-induced angiogenesis in vascular endothelial cells

Comunicaciones a congreso

Alinhamento dos padrões de desempenho docente e o programa de estudos de pré-escola mexicano

In a school system oriented to the fulfillment of educational objectives and the improvement of teaching performance, the congruence between the normative documents and the evaluation of teaching is fundamental. From this perspective, the objective of this study was to analyze the alignment between the implicit teaching competencies of the Preschool Studies Program and the performance teaching standards, as well as assessing the agreement between two versions of the standards issued by the Mexican Ministry of Education. To address those objectives the methodology included two descriptive studies, which apply content analysis techniques as well as work with subject matters judges, the curriculum alignment technique and Bloom's revised taxonomy. In general, the standards are aligned with the Program, except in the areas of formative evaluation and the use of play as a didactic strategy; in the last version of the standards the demands of the cognitive process were lower in the taxonomy. The conclusion suggests an integrated evaluation system that recognizes all specific aspects of preschool teaching practice.En un sistema escolar orientado al cumplimiento de los objetivos educativos y a la mejora del desempeño docente, la congruencia entre los documentos normativos y la evaluación docente es fundamental. Desde esta perspectiva, el objetivo general del presente trabajo fue analizar la alineación entre las competencias docentes implícitas en el Programa de Estudios de Preescolar (PEP) y los estándares de evaluación del desempeño docente en México, así como valorar la concordancia entre dos versiones de los estándares emitidos por la Secretaría de Educación Pública. El método incluyó dos estudios descriptivos, en los cuales se utilizaron: técnicas de análisis de contenido, jueceo por expertos, el método de alineamiento curricular y la taxonomía revisada de Bloom. En general se encontró alineamiento entre los estándares y el PEP, excepto en evaluación formativa y el uso del juego como estrategia didáctica; en la última versión de los estándares, las demandas del proceso cognitivo se bajaron de nivel taxonómico. Se concluye a favor de un sistema integrado de evaluación que reconozca los aspectos particulares de la práctica docente en preescolar.Em um sistema escolar orientado ao cumprimento dos objetivos educativos e à melhoria no desempenho docente, a congruência entre os documentos normativos e a avaliação docente é fundamental. A partir desta perspetiva, o objetivo geral do presente trabalho foi analisar o alinhamento entre as competências docentes implícitas no Programa de Estudos de Pré-escola (PEP) e os padrões de avaliação do desempenho docente no México, assim como valorizar a concordância entre duas versões dos padrões emitidos pela Secretaria de Educação Pública. O método incluiu dois estudos descritivos, nos quais se utilizaram: técnicas de análase de conteúdo, validação por especialistas, o método de alinhamento curricular e a Taxonomia revisada de Bloom.  Geralmente encontrou-se alinhamento entre os padrões e o PEP, exceto na avaliação formativa e o uso de jogos como estrátegia didática; na última versão dos padrões, as exigencias do processo cognitivo baixaram de nível taxonômico. Conclui-se a favor de um sistema integrado de avaliação que reconheça os aspectos particulares da prática docente na pré-escola