Physiologic and morphologic evaluation of the masticatory system in children

Orientador: Maria Beatriz Duarte GaviãoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Diversos são os fatores que parecem interferir na mastigação: força de mordida (que pode estar relacionada ao tamanho dos músculos), componentes e quantidade de saliva, características da oclusão, dentre outros. Esta tese está composta de cinco capítulos. O capítulo 1 "The use of ultrasound in the investigation of the muscles of mastication" revisou a literatura a respeito das imagens ultrasonográficas (US) dos músculos da mastigação e reforçou que a US é um método confiável de grande utilidade no campo do diagnóstico de alterações dos músculos da mastigação e do estudo de mudanças durante o crescimento e envelhecimento e que permanece como uma técnica promissora para o estudo dos músculos da mastigação. O capítulo 2, "Correlation between muscle thickness and bite force in children before and after oral rehabilitation - a 2 year logitudinal study", avaliou a correlação entre força de mordida (FM) e espessura muscular antes e após 6, 12 e 24 meses de reabilitação bucal (T0, T6, T12, T24, respectivamente) em 19 crianças de 6-9 anos que apresentavam perda precoce de dentes decíduos. A FM foi determinada com um transdutor pressurizado flexível. A espessura dos músculos masseter e temporal foi mensurada durante relaxamento e máxima intercuspidação com US digital com transdutor linear de 56mm/10-MHz. Houve aumento significativo da FM e espessura do masseter entre T0 e T12. A FM e a espessura do temporal relaxado e contraído em T6 e temporal contraído em T24 foram significativamente correlacionadas. Foi concluído que houve aumento da FM e espessura do masseter após a reabilitação e também relação entre FM e espessura do temporal, o que sugere que a reabilitação bucal influenciou os aspectos morfológicos e funcionais dos músculos da mastigação. Os capítulos 3 "Bite force and salivary flow rate in schoolchildren", 4 "Bite force, texture perception and chewing parameters in schoolchildren" e 5 "Salivary flow rate, protein content, chewing parameters, and taste perception in schoolchildren" tiveram como objetivos avaliar e correlacionar o fluxo salivar (FS), FM, parâmetros de mastigação, percepção de sabor e textura de alimentos e o papel da proteína na mastigação em crianças de 7-10 anos de idade distribuídas de acordo com fase da dentição, oclusão e sexo. A saliva total não estimulada (NE) e estimulada (E) por parafilm foram coletadas por 5 minutos para obtenção do FS. A proteína total foi avaliada pelo método de Lowry et al. (1951). A FM foi determinada como um transdutor pressurizado flexível. Os seguintes alimentos foram testados: cenoura, torrada com e sem margarina, banana, queijo e goma de mascar para determinar os parâmetros mastigatórios (tempo de mastigação, número e freqüência de ciclos mastigatórios), a textura (utilizando uma escala visual analógica de 0 a 100 mm) e o sabor (classificado como amargo, azedo, salgado ou doce). Meninos apresentaram FS e FM (Capítulo 3) significativamente maior que as meninas. Houve correlação significativa entre FS e FM para o grupo com oclusão normal. Não houve diferença significativa na FM, textura ou nos parâmetros da mastigação entre os grupos de oclusão. Os parâmetros da mastigação e a textura foram significativamente menores para os alimentos macios em relação aos duros. Correlação significativa foi encontrada entre FM e freqüência mastigatória no grupo com oclusão normal. Houve correlação significativa entre o FS e os parâmetros de mastigação. Meninas, crianças com maior FS e menor conteúdo de proteína tiveram maior porcentagem de acerto no sabor dos alimentos. Concluindo, houve relação entre FM e FS em crianças com oclusão normal, nas quais observou-se que a maior FM está relacionada a maior freqüência de mastigação de alimentos, o que indica facilidade de mastigação; o FS maior facilitou a mastigação e percepção do sabor de alimentos.Abstract: Several are the factors that seem to intefeer on mastication: bite force (which might be related to the size of the muscles), components and quatity of saliva, occlusion caracteristics, among other. This thesis is composed by five chapters. Chapter 1 "The use of ultrasound in the investigation of the muscles of mastication" did a literature review about the use of ultrasonography on the investigation of the muscles of mastication and reinforced that US is a reliable method of great utility in the diagnostic field of alterations of the muscles of mastication and in the study of changes during growth and aging and that it remains as a promising technique for the study of the muscles of mastication. Chapter 2, "Correlation between muscle thickness and bite force in children before and after oral rehabilitation - a 2 year logitudinal study", evaluated the correlation between BF and muscle thickness before and after 6, 12, and 24 months of oral rehabilitation (TO, T6, T12, T24, respectively) in 19 6-9 years-old children with early loss of primary teeth. BF was determined with a flexible pressurized transducer. Masseter and temporal muscle thickness was measured during relaxation and maximal intercuspation by digital US with a high- resolution real-time 56mm/10-MHz linear-array transducer. There was a significant increase in BF and masseter thickness between TO and T12. There was a significant correlation between BF and relaxed and contracted temporal thickness at T6, and contracted temporal at T24. It was concluded that there was an increase in BF and masseter thickness after rehabilitation and there was also a relation between BF and temporal thickness, which suggests that the oral rehabilitation influences the morphological and functional aspects of the muscles of mastication. Chapters 3 "Bite force and salivary flow rate in schoolchildren", 4 "Bite force, texture perception and chewing parameters in schoolchildren" and 5 "Salivary flow rate, protein content, chewing parameters, and taste perception in schoolchildren" aimed to evaluate and correlate the salivary flow rate (SFR), BF, chewing parameters, perception of taste and texture of food, and the role of protein on mastication of children 7-10 years-old distributed according to occlusion and sex. BF was determined with a flexible pressurized transducer. Unstimulated (U) and stimulated (S) whole saliva were collected for 5 minutes in order to stablish the salivary flow rate (SFR), and the total protein was evaluated by method of Lowry et al. (1951). The following foods were tested: carrot, toast with and without margarine, banana, cheese, and chewing gum to determine the chewing parameters (chewing time, number of cycles and frequency), texture (using a visual analogue scale from 0 to 100 mm) and the taste (bitter, sour, sweet or salty). Boys presented SFR and BF (Chapter 3) significantly higher than girls. There was a significant correlation between SFR and BF for the group with normal occlusion. There was no significant difference in BF, texture or chewing parameters among groups of occlusion. Chewing parameters and texture were significantly lower for soft than hard foods. Significant correlation was found for BF and chewing frequency in the normal occlusion group. There was significant correlation between SFR and chewing parameters. Girls, children with higher SFR and lower protein content got more correct taste choices. Concluding, there was a relation between SFR and BF in children with normal occlusion, on whom a greater BF was related to a higher chewing frequency, which indicates facility of chewing; a higher SFR facilitated the mastication and taste perception of foods.DoutoradoOdontopediatriaDoutor em Odontologi

Restaurant-OnLine

Aquesta és una aplicació de comerç electrònic que, encara que no inclou la possibilitat de realitzar el pagament en línia, ofereix una alternativa a la realització de comandes a establiments de restauració. S'ha utilitzat l'arquitectura J2EE.Esta es una aplicación de comercio electrónico que, aunque no incluye la posibilidad de realizar el pago online, ofrece una alternativa a la realización de pedidos a establecimientos de restauración. Se ha utilizado la arquitectura J2EE.This is an e-commerce application that, although it does not include the possibility of online payment, offers an alternative to placing orders to catering establishments. J2EE architecture was used

Default Mode Network structural alterations in Kocher-Monro trajectory white matter transection: A 3 and 7 tesla simulation modeling approach

The Kocher-Monro trajectory to the cerebral ventricular system represents one of the most common surgical procedures in the field of neurosurgery. Several studies have analyzed the specific white matter disruption produced during this intervention, which has no reported adverse neurological outcomes. In this study, a graph-theoretical approach was applied to quantify the structural alterations in whole-brain level connectivity. To this end, 132 subjects were randomly selected from the Human Connectome Project dataset and used to create 3 independent 44 subjects groups. Two of the groups underwent a simulated left/right Kocher-Monro trajectory and the third was kept as a control group. For the right Kocher-Monro approach, the nodal analysis revealed decreased strength in the anterior cingulate gyrus of the transected hemisphere. The network-based statistic analysis revealed a set of right lateralized subnetworks with decreased connectivity strength that is consistent with a subset of the Default Mode Network, Salience Network, and Cingulo-Opercular Network. These findings could allow for a better understanding of structural alterations caused by Kocher-Monro approaches that could reveal previously undetected clinical alterations and inform the process of designing safer and less invasive cerebral ventricular approaches

First Record of Loricariichthys edentatus (Siluriformes: Loricariidae) in the Paraná River

Campañas realizadas al noreste de Argentina en el río Paraná revelaron la presencia de la vieja del agua Loricariichthys edentatus Reis & Pereira 2000, que representa el primer registro de esta especie para esa cuenca.Recent expeditions to northeastern Argentina in the Paraná River revealed the presence of the armored catfish Loricariichthys edentatus Reis & Pereira 2000, which represents the first record of this species to that basin.Fil: Teran, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Aguilera, Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Serra, Wilson Sebastián. Museo Nacional de Historia Natural; UruguayFil: Ruiz Diaz, Federico Jose. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mirande, Juan Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentin

Association of common polymorphisms in the VEGFA and SIRT1 genes with type 2 diabetes-related traits in Mexicans

Introduction: Genetic variants have been replicated for association with type 2 diabetes mellitus (T2D) and many of them with diabetes-related traits. Because T2D is highly prevalent in Mexico, this study aimed to test the association of CDKN2A/B, PPARGC1A, VEGFA, SIRT1 and UCP2 gene polymorphisms (rs10811661, rs8192678, rs2010963, rs7896005 and rs659366 respectively) with metabolic traits in 415 unrelated Mexican mestizos with T2D under three models of inheritance. Material and methods: A total of 415 unrelated Mexican mestizos were genotyped by TaqMan assays. Triglycerides, cholesterol, glucose, high-density lipoprotein cholesterol (HDL-C), insulin and anthropometric measurements were determined and the HOMA-IR was calculated. Association studies were tested by the Kruskal-Wallis test, linear regression, statistical power analysis, Bonferroni correction, paired SNP analysis, and physical interaction by GeneMANIA. Results: All polymorphisms were in Hardy-Weinberg equilibrium, and the association by genotype with T2D-related traits displayed nominal significance for rs8192678 with glucose (p = 0.023) and triglycerides (p = 0.013); rs2010963 with diastolic blood pressure (DBP) (p = 0.012) and cholesterol (p = 0.013); rs7896005 with DBP (p = 0.012) and insulin (p = 0.011); and rs659366 with cholesterol (p = 0.034), glucose (p = 0.031) and triglycerides (p = 0.028); and the association of rs2010963 with HDL-C (p = 0.0007) was significant. Linear regression performed with three models of inheritance, adjusted by age + sex + BMI and corrected with Bonferroni, showed a significant association of rs2010963 with HDL-C in an additive model (p = 0.007); and rs7896005 was significantly associated with DBP in the recessive model (p = 0.006). Conclusions: Rigorous analysis evidenced the association of VEGFA rs2010963 and SIRT1 rs7896005 with HDL-C and DBP respectively; these traits are known predictors of cardiovascular complications, which increase the risk of cardiovascular diseases in this population

On the presence of the poorly documented Hypostomus isbrueckeri Reis, Weber & Malabarba, 1990 (Siluriformes: Loricariidae) in Argentina and extension of its distribution range

Hypostomus Lacépède is the most speciose loricariid genus, including about 150 valid species (Fricke et al. 2022). It has a wide distribution in the Neotropical basins, occurring from Central America to the Salado River, South of the La Plata River in the Province of Buenos Aires, Argentina (Ringuelet 1975; López 1992; Fricke et al. 2022). In Argentina the genus was recorded for the first time in 1876 (Weyenbergh 1876; Koerber & Weber 2014), and there are currently 26 species considered as present or probably present in the country (Cardoso et al. 2011, 2012, 2016, 2019; Koerber & Weber 2014; Terán et al. 2020). Some of these species are poorly documented in the literature since the records lack traceable voucher specimens, detailed descriptions and photographs, necessary conditions to make reliable and falsifiable faunal records (Serra et al. 2021). In recent collection campaigns in the Uruguay river basin in the province of Corrientes (Argentina), we collected specimens of Hypostomus that were identified by comparison with collection specimens and literature information.Fil: Teran, Guillermo Enrique. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Serra, Wilson Sebastián. Ministerio de Educación y Cultura. Museo Nacional de Historia Natural; Uruguay. Centro Universitario Regional Este; UruguayFil: Ruiz Diaz, Federico Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Bio y Geociencias del NOA. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Museo de Ciencias Naturales. Instituto de Bio y Geociencias del NOA; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Alonso, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Bio y Geociencias del NOA. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Museo de Ciencias Naturales. Instituto de Bio y Geociencias del NOA; ArgentinaFil: Bugeau, Horacio Baltazar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Aguilera, Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Mirande, Juan Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentin

A singular position-dependent mass particle in an infinite potential well

We consider an unusual singular position=dependent-mass particle in an infinite potential well. The corresponding Hamiltonian is mapped through a point-canonical-transformation and an explicit correspondence between the target Hamiltonian and a Poschl-Teller type reference Hamiltonian is obtained. New ordering ambiguity parametric setting are suggested.Comment: 13 pages, no figures. To appear in Phys. Lett.

Distribution of Six Polymorphisms in Two Communities with a Historical High Incidence of Diabetes and Obesity in Yucatan, Mexico

Aims: Maya ancestry populations from Yucatan have exhibited a high prevalence of diabetes and obesity; consequently, the aim of this study was to determine the allelic and genotype frequencies of six polymorphisms associated with diabetes and obesity in two Maya populations. Place and Duration of Study: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN; Laboratorios de Genética y Hematología, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán between September 2014 and March 2016. Methodology: Healthy individuals with Maya ancestry were recruited in small rural and urban communities from Yucatan. Six polymorphisms present in five genes (PPARGC1A, NRF1, SLC30A8, ADRA2A and UCP3) were genotyped using TaqMan assays. Linkage disequilibrium analysis was performed for rs13266634 and rs11558471 (SLC30A8). Results: The observed frequencies in the small rural community (SRC) and Merida were in Hardy Weinberg equilibrium. Frequencies of five polymorphisms (rs8192678, rs1882095, rs13266634, rs11558471 and rs3781907) correlated with 1000 genomes project data, furthermore, statistical analysis did not reveal a significant difference between genotype frequencies of the SRC and Merida populations. Contrary, frequencies from Mexicans living in Los Angeles compared with frequencies obtained in Yucatan (SRC and Merida) indicated significant difference on genotype frequencies in the ADRA2A gene (P-value= .017). The polymorphisms rs13266634 and rs11558471 in the SLC30A8 gene displayed strong linkage disequilibrium (D’= 0.96), displaying frequencies of 0.725, 0.255, and 0.015 for haplotypes C-A, T-G, and C-G respectively. Conclusion: Distribution of A allele in rs553668 (ADRA2A) in the Yucatan populations was higher than the frequency reported for Mexicans from LA, Americans, Europeans, and Africans. This finding could be related to blood pressure levels in the Maya populations. Additionally, a high frequency of C-A haplotype (rs13266634 and rs11558471) in the SLC30A8 gene could be associated with an increased risk of diabetes and obesity in these populations