Distinct regulation of glucose transport and GLUT1/GLUT3 transporters by glucose deprivation and IGF-I in chromaffin cells

AbstractEffects of prolonged metabolic (glucose deprivation) and hormonal [insulin-like growth factor I (IGF-I)] challenge on regulation of glucose transporter (GLUT) expression, glucose transport rate and possible signaling pathways involved were studied in the neuroendocrine chromaffin cell. The results show that bovine chromaffin cells express both GLUT1 and GLUT3. Glucose deprivation and IGF-I activation led to an elevation of GLUT1 and GLUT3 mRNA, the strongest effect being that of IGF-I on GLUT3 mRNA. Both types of stimulus increased the GLUT1 protein content in a cycloheximide (CHX)-sensitive manner, and the glucose transport rate was elevated by 3- to 4-fold after 48 h under both experimental conditions. IGF-I-induced glucose uptake was totally suppressed by CHX. In contrast, only ∼50% of transport activation in glucose-deprived cells was sensitive to the protein synthesis inhibitor. Specific inhibitors of mTOR/FRAP and p38 MAPK each partially blocked IGF-I-stimulated glucose transport, but had no effect on transport rate in glucose-deprived cells. The results are consistent with IGF-I-activated transport being completely dependent on new GLUT protein synthesis while the enhanced transport in glucose-deprived cells was partially achieved independent of new synthesis of proteins, suggesting a mechanism relying on preexisting transporters

Témoignage à propos du traitement des sculptures polychromées en France. Une spécialité récente

L’évolution du métier de conservateur-restaurateur de sculptures polychromées est décrite. La conception de la restauration scientifique naît en Allemagne, se poursuit en Belgique et atteint ensuite la France. Les personnalités qui établissent la méthodologie et la déontologie du métier devenu spécifique sont citées. Les difficultés à appliquer ces théories sont soulignées. La naissance des institutions internationales pour la conservation du patrimoine d’une part et des écoles spécialisées d’autre part permettront la diffusion des meilleures pratiques en restauration des sculptures en Europe. L’évolution remarquable des méthodes de laboratoires et de traitement en matière de nettoyage et de dégagement de la polychromie permettent une amélioration très positive du métier et de la protection de ce type d’œuvres d’art au xxie siècle.The evolution of the profession of conservator-restorer of painted wooden sculptures is described in this paper. The concept of scientific restoration saw the day in Germany, then spread to Belgium before arriving in France. The people who established the methodology and deontology of this henceforth specific profession are cited. The difficulties that lie in applying these theories are underlined. The emergence of international institutions for the conservation of national heritage, on the one hand, and specialized schools, on the other, will enable better practices in sculpture restoration to become widespread in Europe.The remarkable advances in laboratory methods and treatments for cleaning works and uncovering polychromy have led to very positive improvements in the profession and in the protection of these types of works in the 21st century

El brocado de estaño en relieve aplicado. Evolución histórica y material en la Europa medieval con atención al arte español (siglos XV-XVI)

The term applied tin-relief brocade (commonly called applied brocade) refers to painted decoration that simulates the velvet and silk brocades made of gold and silver threads commonly used in Europe in the fifteenth and sixteenth centuries. This technique uses a mould, tin leaf, and filling mass to reproduce in a mechanical way the relief motifs of textile brocades. Once cut, they are applied to artwork, and then sometimes gilded or painted. The discovery of many examples of this type of decoration in northern Spain has led us to the study of the documentary sources, especially Spanish ones, with the purpose of improving our understanding of the technique, its historic and material development, and the typology of standardized patterns. Furthermore, this study has provided us with an important glossary of the relief painting additive techniques that culminated in applied tinrelief brocade.El término brocado de estaño en relieve aplicado (comúnmente conocido como brocado aplicado) se refiere a una decoración polícroma que imita los brocados de terciopelo y seda hechos con hilos de oro y plata, los cuales dominan el mundo de la moda de Europa en los siglos XV y XVI. Esta técnica reproduce mecánicamente a través de una matriz, lámina de estaño y masa de relleno motivos en relieve de brocados textiles, que, una vez recortados, son aplicados sobre la obra, pudiendo estar en ocasiones dorados y pintados. El hallazgo de más ejemplos de esta decoración en el Norte de España nos ha conducido a analizar las fuentes documentales, especialmente las españolas, con el objetivo de ampliar nuestro conocimiento sobre la técnica en cuestión, su evolución histórica y material y sus diferentes tipologías. Asimismo, dicho estudio nos ha proporcionado un importante glosario de técnicas pictóricas en relieve por adición precursoras del brocado de estaño en relieve aplicado

M & L Jaargang 19/6

Anna Bergmans De Sint-Martinusbasiliek van Halle. Onderzoek en restauratie van kunstwerken. [Saint Martins basilica in Halle Study and restoration of works of art.]Myriam Serck Het gotische triomfkruis. Onderzoek en conservering. [The Gothic Cross of Triumph Research and conservation.]Aletta Rambaut 15de en 17de-eeuws gebrandschilderd glas-in-lood uit de doopkapel. [The 15th and 17th century stained-glass windows of the baptistery.]Isabelle Leirens Het zuidportaal: technische studie, conservatie en restauratie. [The South portal: Technical study, conservation and restoration.]Ann Peckstadt Conservering van een calque, genomen van een middeleeuwse muurschildering: de Annunciatie (circa 1400). [Conservation of a tracing, taken from a mediaeval wall painting: the Annunciation (appr. 1400).]Rie Vermeiren Het archief van de ateliers Bressers en Blanchaert. [The archives of the workshops of Bressers and Blanchaert.]Summar

Splenic infarction as a complication of celiac artery thromboembolism: an unusual cause of abdominal pain

Splenic infarction is a relatively uncommon diagnosis and this clinical presentation can mimic other causes of acute abdominal pain. Cardiologic and hematologic disorders are common reasons for this entity. There have been a few series and single case reports of splenic infarction published in peer-reviewed medical journals. We report a 53-year-old patient who had splenic infarction caused by celiac artery thromboembolism. The importance of this case, without any etiological predisposing factors, is that this kind of clinical situation should be considered in the differential diagnosis of abdominal pain

M & L Jaargang 18/2

E. Ignace De Wilde Boerenkrijgmonumenten van 1898. [Memorials to the Peasantsrevolt of 1898.]Antoon Fauconnier en Patrick Roose Een van Peteghem-orgel uit Diest in de kerk van de Landcommanderij Alden Biesen. [A Van Peteghem organ from Diest in the land&nbspcommandery Alden Biesen.]Anne-Sophie Augustyniak, Ingrid Geelen en Myriam Serck-Dewaide Het laatgotisch Sint-Annaretabel (1533) van de Sint-Salvatorskathedraal te Brugge. [The late gothic Saint Annas retable in Bruges St. Salvators cathedral.]Summar