High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata

In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts. Callus cultures were initiated using leaves collected from shoot cultures and the root tips from hairy root cultures obtained upon Agrobacterium rhizogenes transformation. For the isolation of protoplast, the different explant material of M. lanceolata was exposed to an enzyme mixture consisting of 1.5% cellulase, 0.5% macerozyme R-10 and 0.5 M mannitol. About 6 x 106 protoplasts g-1 fresh weight were obtained from leaf material and 5 x 105 protoplasts g-1 fresh weight from callus. To obtain high amounts of hairy root protoplasts, the cultures were pretreated with the auxin indole-3-butyric acid (IBA) that stimulated the formation of novel root tips. Using the dissected root tips as starting material, 8 x 105 protoplasts g-1 fresh weight were obtained per preparation. The protoplast isolation method will enable further studies on the transformation and fusion of protoplasts from M. lanceolata

Odd circuits in dense binary matroids

We show that, for each real number $\alpha > 0$ and odd integer $k\ge 5$ there is an integer $c$ such that, if $M$ is a simple binary matroid with $|M| \ge \alpha 2^{r(M)}$ and with no $k$-element circuit, then $M$ has critical number at most $c$. The result is an easy application of a regularity lemma for finite abelian groups due to Green

Projective geometries in exponentially dense matroids. I

We show for each positive integer $a$ that, if \cM is a minor-closed class of matroids not containing all rank-$(a+1)$ uniform matroids, then there exists an integer $n$ such that either every rank-$r$ matroid in \cM can be covered by at most $r^n$ sets of rank at most $a$, or \cM contains the \GF(q)-representable matroids for some prime power $q$, and every rank-$r$ matroid in \cM can be covered by at most $r^nq^r$ sets of rank at most $a$. This determines the maximum density of the matroids in \cM up to a polynomial factor

Studies on saponin production in tropical medicinal plants Maesa argentea and Maesa lanceolata

The continuous need for new compounds with important medicinal activities has lead to the identification and characterization of various plant-derived natural products. As a part of this program, we studied the saponin production from two tropical medicinal plants Maesa argentea and M. lanceolata and evaluated several treatments to enhance their saponin production. In this experiment, we present the analyses of saponin production from greenhouse grown plants by means of TLC and HPLC-MS. We observed that the content of saponin from these plants varied depending on organ and physiological age of the plants. In addition, the impact of elicitors on saponin accumulation on in vitro grown plants was analyzed using TLC. The production of saponin was very stable and not affected by treatment with methyl jasmonate, and salicylic acid. In conclusion, Maesa saponins are constitutively produced in plants and the level of these compounds in plants is mainly affected by the developmental or physiological stage