Historical fire records at the two ends of Iberian Central Mountain system: Estrela massif and Ayllon massif

The Iberian Peninsula has a long history of fire, as the Central Mountain System, from the Estrela massif in Portugal to the Ayllón massif in Spain, is a major fire-prone area. Despite being part of the same natural region, there are different environmental, political and socio-economic contexts at either end, which might have led to distinct human causes of wildfires and associated fire regimes. The hypothesis for this research lies in the historical long-term relationship between wildfire risks and fire use practices within a context of landscape dynamics. In addition to conducting an analysis of the statistical period, a spatial and temporal multiscale approach was taken by reconstructing the historical record of prestatistical fires and land management history at both ends of the Central Mountain System. The main result is the different structural causes of wildland fires at either end of the Central Mountain System, with human factors being more important than environmental factors in determining the fire regimes in both contexts. The study shows that the development of the fire regime was non-linear in the nineteenth and twentieth centuries, due to broader local human context factors which led to a shift in fire-use practicesLa Península Ibérica cuenta con una larga historia de incendios forestales. Es el caso del Sistema Central, desde la Sierra de Estrela en Portugal a la Sierra de Ayllón en España, aunque las causas humanas y el régimen de incendios difieren en función del contexto ambiental, político y socioeconómico en uno y otro extremo de la cordillera. La validación de la hipótesis de trabajo, sobre la relación histórica entre el riesgo de incendios y el uso del fuego en las actividades humanas, se ha llevado a partir de la reconstrucción del registro histórico de incendios forestales y de la gestión del territorio, y mediante el análisis multiescalar espacio- temporal de los incendios históricos y estadísticos. Como principal resultado se han identificado las causas estructurales de incendios en las sierras de Estrela y Ayllón. Además, se ha demostrado la influencia mayor de los aspectos humanos que de los físicos en la evolución del régimen de fuego. En conclusión, este trabajo evidencia la evolución discontinua de los incendios forestales a lo largo de los siglos XIX y XX debido a los factores contextuales humanos que influyen en el manejo tradicional del fuego a escala localinfo:eu-repo/semantics/publishedVersio

Have historical land use/land cover changes triggered a fire regime shift in central Spain?

Fire is one of the main disturbance factors shaping the landscape, and landscape is a key driver of fire behavior. Considering the role played by land use and land cover (LULC) changes as the main driver of landscape dynamics, the aim of this study was to calculate and analyze (i) the real impact of fire on LULC changes and (ii) how these LULC changes were influencing the fire regime. We used methods of historical geography and socio-spatial systemic analysis for reconstructing and assessing the LULC change and fire history in six case studies in the Central Mountain System (Spain) from archival documentary sources and historical cartography. The main result is an accurate dataset of fire records from 1497 to 2013 and a set of LULC maps for three time points (1890s–1930s, 1956–1957, and the 2000s). We have shown the nonlinear evolution of the fire regime and the importance of the local scale when assessing the interaction of landscape dynamics and fire regime variation. Our findings suggest that LULC trends have been the main influencing factor of fire regime variation in Central Spain since the mid-19th centuryinfo:eu-repo/semantics/publishedVersio

Recurrent duplications of the annexin A1 gene (ANXA1) in autism spectrum disorders

Validating the potential pathogenicity of copy number variants (CNVs) identified in genome-wide studies of autism spectrum disorders (ASD) requires detailed assessment of case/control frequencies, inheritance patterns, clinical correlations, and functional impact. Here, we characterize a small recurrent duplication in the annexin A1 (ANXA1) gene, identified by the Autism Genome Project (AGP) study

PKC alpha-Specific Phosphorylation of the Troponin Complex in Human Myocardium: A Functional and Proteomics Analysis

Aims:Protein kinase Cα (PKCα) is one of the predominant PKC isoforms that phosphorylate cardiac troponin. PKCα is implicated in heart failure and serves as a potential therapeutic target, however, the exact consequences for contractile function in human myocardium are unclear. This study aimed to investigate the effects of PKCα phosphorylation of cardiac troponin (cTn) on myofilament function in human failing cardiomyocytes and to resolve the potential targets involved.Methods and Results:Endogenous cTn from permeabilized cardiomyocytes from patients with end-stage idiopathic dilated cardiomyopathy was exchanged (∼69%) with PKCα-treated recombinant human cTn (cTn (DD+PKCα)). This complex has Ser23/24 on cTnI mutated into aspartic acids (D) to rule out in vitro cross-phosphorylation of the PKA sites by PKCα. Isometric force was measured at various [C

Molecular typing of dengue virus type 2 in Brazil Tipificação molecular do vírus da dengue tipo 2 no Brasil

Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).<br>A tipagem de cepas é uma ferramenta fundamental para a análise epidemiológica molecular e pode fornecer informações importantes sobre a dispersão dos vírus dengue. Neste trabalho, foi realizada a caracterização molecular de amostras de vírus DEN-2 isoladas no Brasil entre 1990-2000, de áreas geograficamente e temporalmente distintas, com o objetivo de investigar a distribuição genética deste sorotipo circulante no país. A reação em cadeia pela polimerase baseada em sítios de restrição específicos (RSS-PCR) apresentou o mesmo padrão para as 52 amostras Brasileiras, mostrando a circulação de apenas uma variante de vírus DEN-2. A análise filogenética utilizando alinhamento progressivo de sequências de 240 nucleotídeos da junção E/NS1 de 15 cepas mostrou que estas pertencem ao genotipo III (genotipo Jamaica)