Propuesta de un plan de produccion limpia para la optimizacion de recursos en Coca-Cola Embonor S.A., Planta Talca.

150 p.Coca-Cola Embonor S.A. es una compañía dedicada a la producción de bebidas carbonatadas, bebidas no carbonatadas y aguas (minerales y purificadas) para el consumo humano. Su constante preocupación por estar a la vanguardia, debido a su condición de empresa líder en el mercado, la ha llevado a la idea de mantener un programa de uso racionado de recursos, ya que estos son escasos en la sociedad actual. El objetivo de esta memoria es proponer la implementación de un Plan de optimización de agua, energía y la disposición de residuos, es por esto que el trabajo se realizó de manera que este fuera desarrollado de la forma más adecuada. A través de lo que se presenta en los tres primeros capítulos, con la finalidad de realizar un Plan de manejo de recursos no renovables (agua y energía) y disposición final de residuos, se comienza a conocer, de manera general, el funcionamiento de la Planta. También se dan a conocer la situación actual en que se encuentra la Planta, presentando los tipos de agua (potable, tratada, blanda clorada y blanda sin cloro) que se producen y energía (eléctrica y térmica) que se utilizan, las normas asociadas a residuos Líquidos y Sólidos,elementos importantes al momento de determinar el diagnóstico. Otra parte del diagnóstico, que es relevante al momento de plantear las propuestas, son las mediciones realizadas dentro de la Planta, estas se describen durante este cuarto capítulo de forma detallada. Dando énfasis en la identificación de los lugares donde ocurren las pérdidas de los recursos y las cantidades utilizadas en cada uno de ellos. Aquí queda clara la importancia de llevar registros de los consumos. Una vez presentado el diagnóstico se dan a conocer las áreas de oportunidad más factibles de realizar, enfocado en los principios de la Producción Limpia los cuales plantean como primera opción la reducción en el origen y posteriormente la valorización de residuos mediante la reutilización o reciclado. Mediante las mejoras propuestas se estima que la empresa disminuya el ratio de agua a un 119% y el Ratio de Energía en un 155%, la disminución de ambos Ratios es significativa, según lo indicado por la Compañía, y se espera reducir los costos de operación en más de 2 millones de pesos

La cultura profesional de los docentes en enseñanza secundaria: Un estudio biográfico.

This work discusses the "voices" of teachers working "at the chalk face." We are interested in learning what teachers think and how they behave. Therefore, we conducted our research about their working conditions. We also developed teachers" professional biographies to learn how these educators construct concepts and practices related to their profession and what constitutes their "professional culture". We understand that thie professional culture characterizes teachers" professional frameworks and choices; therefore, any attempt to successfully promote an educational reform, must understand and pay attention to the "professional culture."Este trabajo trata sobre las "voces" de los profesores que trabajan "a pie de obra". Nos interesa aprender qué piensan los profesores y cómo se comportan. Para ello se ha llevado a cabo una investigación en sus propios contextos de trabajo. De este modo, elaboramos sus biografías profesionales para aprender como han ido construyendo estos conceptos y prácticas profesionales y, de este modo, aprender qué es lo que constituye su "cultura profesional". Entendemos que es esta cultura profesional la que determina las opciones de los profesores que caracteriza su estructura profesional y por tanto, cualquier intento de afrontar una reforma educativa necesariamente tiene que atender y entender estas estructuras

El conocimiento práctico en las maestras de 4º curso de Educación Infantil: distancia entre las teorías proclamadas y las teorías al uso

Actualmente los y las docentes deben ser profesionales competentes que den respuestas a las demandas educativas que se les plantean, asuman un rol docente polivalente, y sean conscientes del conocimiento práctico y pensamiento práctico que ponen en juego en su actividad diaria. Este trabajo forma parte de una investigación más extensa subvencionada por una convocatoria de ayuda a proyectos de I+D+i1 . En este caso, se pretende conocer las discrepancias existentes entre lo que los/as futuros/as docentes de Educación Infantil piensan que deben hacer, y lo que hacen, es decir, las contradicciones que existen entre las teorías proclamadas (pensamiento práctico) y las teorías al uso (conocimiento práctico). Para ello se ha realizado un estudio cualitativo, fundamentado en el estudio de casos, en el cual han participado 3 estudiantes de último curso, 3 docentes del Grado de Maestro/a en Educación Infantil, y un responsable del Equipo Decanal de la Universidad de Huelva. Los resultados ponen de manifiesto que, la interacción entre el discurso narrativo de las personas participantes y su verdadera práctica educativa es débil. Debido a esto es necesario plantear estrategias formativas fundamentadas en la reflexión e indagación profunda, donde se produzca una verdadera reconstrucción del conocimiento práctico o conocimiento inconsciente, y se pueden eliminar o acortar la distancia existente entre las teorías al uso y las teorías proclamadas.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1 alpha Upstream of Notch and SUMO Pathways

Mesenchymal stem cells (MSCs) are effective in treating several pathologies. We and others have demonstrated that hypoxia or hypoxia-inducible factor 1 alpha (HIF-1 alpha) stabilization improves several MSC functions, including cell adhesion, migration, and proliferation, thereby increasing their therapeutic potential. To further explore the mechanisms induced by HIF-1 alpha in MSCs, we studied its relationship with Notch signaling and observed that overexpression of HIF-1 alpha in MSCs increased protein levels of the Notch ligands Jagged 1-2 and Delta-like (Dll) 1, Dll3, and Dll4 and potentiated Notch signaling only when this pathway was activated. Crosstalk between HIF and Notch resulted in Notch-dependent migration and spreading of MSCs, which was abolished by gamma-secretase inhibition. However, the HIF-1-induced increase in MSC proliferation was independent of Notch signaling. The ubiquitin family member, small ubiquitin-like modifier (SUMO), has important functions in many cellular processes and increased SUMO1 protein levels have been reported in hypoxia. To investigate the potential involvement of SUMOylation in HIF/Notch crosstalk, we measured general SUMOylation levels and observed increased SUMOylation in HIF-1-expressing MSCs. Moreover, proliferation and migration of MSCs were reduced in the presence of a SUMOylation inhibitor, and this effect was particularly robust in HIF-MSCs. Immunoprecipitation studies demonstrated SUMOylation of the intracellular domain of Notch1 (N1ICD) in HIF-1-expressing MSCs, which contributed to Notch pathway activation and resulted in increased levels of N1ICD nuclear translocation as assessed by subcellular fractionation. SUMOylation of N1ICD was also observed in HEK293T cells with stabilized HIF-1 alpha expression, suggesting that this is a common mechanism in eukaryotic cells. In summary, we describe, for the first time, SUMOylation of N1ICD, which is potentiated by HIF signaling. These phenomena could be relevant for the therapeutic effects of MSCs in hypoxia or under conditions of HIF stabilization.This work was supported, in part, by grants from the Instituto de Salud Carlos III PI13/00414, PI16/0107, RE-TICS RD12/0019/0025 to P.S. and RETICS RD12/0019/0003 (TERCEL) to J.L.D.L.P cofunded by FEDER ``una manera de hacer Europa. It was also supported by the Regenerative Medicine Program of Instituto de Salud Carlos III and Valencian Community to Centro de Investigacion Principe Felipe. The authors are grateful to Dr. A. Dorronsoro for critical review of the work and Dr. K McCreath for manuscript editing.S

Guía de transición para la LOPD: los nuevos derechos

[ES] La necesidad de adaptación de España al Reglamento General de Protección de Datos causó el desarrollo de una nueva ley de protección de datos, llamada Ley Orgánica de Protección de Datos y Garantía de los Derechos Digitales, que se adecuase al reglamento europeo y que garantizase la protección de las personas físicas, en lo que respecta al tratamiento de sus datos personales. Este trabajo proporciona todos los datos necesarios para ser capaz de cumplir la normativa de protección de datos tras los cambios provocados por la entrada en vigor de la Ley Orgánica 3/2018, de 5 de diciembre, de Protección de Datos Personales y Garantía de los Derechos Digitales. Adicionalmente se describe el proceso llevado a cabo para la creación de una lista de tareas pendientes mediante el software de hojas de cálculo llamado “Microsoft Excel”, que pueda ser de utilidad para los encargados de protección de datos.[EN] The need to adapt Spain to the General Data Protection Regulation caused the development of a new data-protection law, called Organic Law on the Protection of Personal Data and the Guarantee of Digital Rights, that fits to the European regulation and guarantees the protection of physical persons, with regard to the processing of their personal data. This work provides all necesary information to be able to follow the data-protection rules after the changes caused by the entry into force of the Spanish Organic Law 3/2018, of 5 december, on the Protection of Personal Data and the Guarantee of Digital Rights. Additionally, it describes the process carried out to create a to-do list using the spreadsheet software called “Microsoft Excel”, which may be useful to processors.Giménez Hervás, C. (2019). Guía de transición para la LOPD: los nuevos derechos. http://hdl.handle.net/10251/128599TFG

Hydrogen Sulfide Improves Cardiomyocyte Function in a Cardiac Arrest Model

[EN] Background: Cardioplegic arrest is a common procedure for many types of cardiac surgery, and different formulations have been proposed to enhance its cardio-protective effect. Hydrogen sulfide is an important signaling molecule that has cardio-protective properties. We therefore studied the cardio-protective effect of hydrogen sulfide in cardiac cell culture and its potential therapeutic use in combination with cardioplegia formulations. Materials/Methods: We added hydrogen sulfide donor GYY4137 to HL-1 cells to study its protective effect in nutrient starved conditions. In addition, we tested the potential use of GYY4137 when it is added into two different cardioplegia formulations: Cardi-Braun (R) solution and del Nido solution in an ex vivo Langendorff perfused rat hearts model. Results: We observed that eight-hour pre-treatment with GYY4137 significantly suppressed apoptosis in nutrient-starved HL-1 cells (28% less compared to untreated cells; p<0.05), maintained ATP content, and reduced protein synthesis. In ex vivo experiments, Cardi-Braun (R) and del Nido cardioplegia solutions supplemented with GYY4137 significantly reduced the pro-apoptotic protein caspase-3 content and preserved ATP content. Furthermore, GYY4137 supplemented cardioplegia solutions decreased the S-(5-adenosyl)-L-methionine/S-(adenosyl)-L-homocysteine ratio, reducing the oxidative stress in cardiac tissue. Finally, heart beating analysis revealed the preservation of the inter-beat interval and the heart rate in del Nido cardioplegia solution supplemented with GYY4137. Conclusions: GYY4137 preconditioning preserved energetic state during starved conditions, attenuating the cardiomyocytes apoptosis in vitro. The addition of GYY4137 to cardioplegia solutions prevented apoptosis, ATP consumption, and oxidative stress in perfused rat hearts, restoring its electrophysiological status after cardiac arrest. These findings suggested that GYY4137 sulfide donor may improve the cardioplegia solution performance during cardiac surgery.Instituto de Salud Carlos III grants (PI10/743, PI13/0414) and RETICS RD12/0019/0025 co-funded by FEDER "Una Manera de Hacer Europa". AG. acknowledges a fellowship from Erasmus Mundus Eurotango Program. ADJ acknowledges support from the Ramon y Cajal program (RYC-2008-02378). P.S. acknowledges support from PI10/743, PI13/414 grants and the Miguel Servet I3SNS and RETICS Program (RD12/0025). We thank Dr Kenneth McCreath for helpful comments on the manuscript.Garcia, NA.; Moncayo-Arlandi, J.; Vázquez Sánchez, A.; Genoves, P.; Calvo Saiz, CJ.; Millet Roig, J.; Martí, N.... (2017). Hydrogen Sulfide Improves Cardiomyocyte Function in a Cardiac Arrest Model. Annals of Transplantation. 22:285-295. https://doi.org/10.12659/AOT.901410S2852952

アトガキ イマ モトメラレル ヨウチエンゾウ キョウドウテキ ナ マナビ ニ ムケテ

Background: Human dental mesenchymal stem cells (MSCs) are considered as highly accessible and attractive MSCs for use in regenerative medicine, yet some of their features are not as well characterized as other MSCs. Hypoxia-preconditioning and hypoxia-inducible factor 1 (HIF-1) alpha overexpression significantly improves MSC therapeutics, but the mechanisms involved are not fully understood. In the present study, we characterize immunomodulatory properties of dental MSCs and determine changes in their ability to modulate adaptive and innate immune populations after HIF-1 alpha overexpression. Methods: Human dental MSCs were stably transduced with green fluorescent protein (GFP-MSCs) or GFP-HIF-1 alpha lentivirus vectors (HIF-MSCs). A hypoxic-like metabolic profile was confirmed by mitochondrial and glycolysis stress test. Capacity of HIF-MSCs to modulate T-cell activation, dendritic cell differentiation, monocyte migration, and polarizations towards macrophages and natural killer (NK) cell lytic activity was assessed by a number of functional assays in co-cultures. The expression of relevant factors were determined by polymerase chain reaction (PCR) analysis and enzyme-linked immunosorbent assay (ELISA). Results: While HIF-1 alpha overexpression did not modify the inhibition of T-cell activation by MSCs, HIF-MSCs impaired dendritic cell differentiation more efficiently. In addition, HIF-MSCs showed a tendency to induce higher attraction of monocytes, which differentiate into suppressor macrophages, and exhibited enhanced resistance to NK cell-mediated lysis, which supports the improved therapeutic capacity of HIF-MSCs. HIF-MSCs also displayed a pro-angiogenic profile characterized by increased expression of CXCL12/SDF1 and CCL5/RANTES and complete loss of CXCL10/IP10 transcription. Conclusions: Immunomodulation and expression of trophic factors by dental MSCs make them perfect candidates for cell therapy. Overexpression of HIF-1 alpha enhances these features and increases their resistance to allogenic NK cell lysis and, hence, their potential in vivo lifespan. Our results further support the use of HIF-1 alpha-expressing dental MSCs for cell therapy in tissue injury and immune disorders

Identifying non-destructive growth and maturity indexes of Prickly pear (Opuntia albicarpa S. Var. Burrona) and evaluation of freeze-drying conditions

Around the world, prickly pear fruits are valued as a source of dietary functional compounds and ingredients for innovative foods. Growth and physicochemical changes of Opuntia albicarpa S. fruits were recorded from 0 to 132 days-after-flowering (DAF) to identify non-destructive maturity-indices. Optimum-ripened fruits were freeze-dried to study physicochemical and functional characteristics of dried and rehydrated pulp. Principal component analysis confirmed growth turned into fruit ripening in DAF 99, and it lasted until DAF 132. Changes in color parameters of the peel correlated with fruit texture and pulp sugar content and taste index (P < 0.01). During freeze-drying, plate temperature had more significant effects than the thickness (P < 0.05). At 30°C, color ΔE between dried and fresh slices augmented, but, texture Δ´s (medium force) between rehydrated and fresh pulp was lower. Color tests could be used to harvest commercially-ripened fruits. Freeze-drying at 30°C improves the rehydrated slices texture regarding thickness maintaining rehydration coefficients.Las tunas son apreciadas en todo el mundo como fuente de compuestos funcionales dietarios e ingredientes para alimentos innovadores. Para identificar índices de maduración no destructivos, se registraron cambios en el crecimiento y en los parámetros fisicoquímicos de frutos de Opuntia albicarpa S. entre los días después de la floración (DAF) 0 al 132. Frutos con maduración óptima fueron liofilizados para evaluar características fisicoquímicas y funcionales de pulpa seca y rehidratada. El análisis de componentes principales confirmo que el crecimiento dio lugar a la maduración en el DAF 99 y ésta prosiguió hasta el DAF 132. Los cambios en parámetros de color en cáscara correlacionaron con la textura del fruto, y en pulpa, con el contenido de azúcares y el índice de sabor (P< 0.01). Durante la liofilización, la temperatura de placa tiene más efectos significativos que el espesor (P≤ 0.05). A 30°C, el ΔE de color entre la pulpa seca y fresca aumentó, pero, el Δ de textura (fuerza media) fue menor entre la rehidratada y la fresca. Se pueden emplear evaluaciones de color para cosechar frutos en su madurez comercial, y liofilizar estos frutos a 30°C mejora la textura sin importar el espesor, manteniendo los coeficientes de rehidratación.This work was supported by the SIMORELOS program of CONACyT

Mutational spectrum of GNAL, THAP1 and TOR1A genes in isolated dystonia: study in a population from Spain and systematic literature review

[Objective] We aimed to investigate the prevalence of TOR1A, GNAL and THAP1 variants as the cause of dystonia in a cohort of Spanish patients with isolated dystonia and in the literature.[Methods] A population of 2028 subjects (including 1053 patients with different subtypes of isolated dystonia and 975 healthy controls) from southern and central Spain was included. The genes TOR1A, THAP1 and GNAL were screened using a combination of high-resolution melting analysis and direct DNA resequencing. In addition, an extensive literature search to identify original articles (published before 10 August 2020) reporting mutations in TOR1A, THAP1 or GNAL associated to dystonia was performed.[Results] Pathogenic or likely pathogenic variants in TOR1A, THAP1 and GNAL were identified in 0.48%, 0.57% and 0.29% of our patients, respectively. Five patients carried the variation p.Glu303del in TOR1A. A very rare variant in GNAL (p.Ser238Asn) was found as a putative risk factor for dystonia. In the literature, variations in TOR1A, THAP1 and GNAL accounted for about 6%, 1.8% and 1.1% of published dystonia patients, respectively.[Conclusions] There is a different genetic contribution to dystonia of these three genes in our patients (about 1.3% of patients) and in the literature (about 3.6% of patients), probably due the high proportion of adult-onset cases in our cohort. As regards age at onset, site of dystonia onset, and final distribution, in our population there is a clear differentiation between DYT-TOR1A and DYT-GNAL, with DYT-THAP1 likely to be an intermediate phenotype.This work was supported by the Carlos III Health Institute-European Regional Development Fund (ISCIII-FEDER) [PI14/01823, PI16/01575, PI18/01898, PI19/01576], the Andalusian Regional Ministry of Economics, Innovation, Science and Employment [CVI-02526, CTS-7685], the Andalusian Regional Ministry of Health and Welfare [PI-0741-2010, PI-0471-2013, PE-0210-2018, PI-0459-2018, PE-0186-2019], and the Alicia Koplowitz and Mutua Madrileña Foundations. Pilar Gómez-Garre was supported by the "Miguel Servet" program [MSII14/00018] (from ISCIII-FEDER) and “Nicolás Monardes” program [C-0048-2017] (from the Andalusian Regional Ministry of Health). Silvia Jesús was supported by the "Juan Rodés" program [B-0007-2019] and Daniel Macías-García by the “Río Hortega” program [CM18/00142] (both from ISCIII-FEDER). María Teresa Periñán was supported by the Spanish Ministry of Education [FPU16/05061]. Cristina Tejera was supported by VPPI-US from the University of Seville.Peer reviewe

Priorities in Cardio-Oncology Basic and Translational Science

Despite improvements in cancer survival, cancer therapy–related cardiovascular toxicity has risen to become a prominent clinical challenge. This has led to the growth of the burgeoning field of cardio-oncology, which aims to advance the cardiovascular health of cancer patients and survivors, through actionable and translatable science. In these Global Cardio-Oncology Symposium 2023 scientific symposium proceedings, we present a focused review on the mechanisms that contribute to common cardiovascular toxicities discussed at this meeting, the ongoing international collaborative efforts to improve patient outcomes, and the bidirectional challenges of translating basic research to clinical care. We acknowledge that there are many additional therapies that are of significance but were not topics of discussion at this symposium. We hope that through this symposium-based review we can highlight the knowledge gaps and clinical priorities to inform the design of future studies that aim to prevent and mitigate cardiovascular disease in cancer patients and survivors.</p