Aplicaciones de las herramientas proteómicas en tecnología de la alimentación

Las técnicas proteómicas se usan cada vez más en la valoración de alimentos tanto procesados como en crudo, así como en el control, la optimización y el desarrollo de nuevos productos en tecnología y biotecnología de alimentos.Peer Reviewe

Empleo del isoelectroenfoque en medio líquido en el fraccionamiento y caracterización de las proteínas del músculo bovino

Comunicaciones a congreso

La Proteómica como vía para determinar el origen animal de los productos cárnicos

Comunicaciones a congreso

Ciencia de la carne y Proteómica

Comunicaciones a congreso

Application of 2-D DIGE to study the effect of ageing on horse meat myofibrillar sub-proteome

Considering the high relevance of meat tenderness for consumer acceptability, the aim of this study was to investigate post-mortem changes in myofibrillar sub-proteome in steaks from longissimus thoracis et lumborum muscle of six Hispano-Bret ' on horses. Indeed, the ageing process that leads to meat tenderization has been scarcely studied in this species. Steaks (n = 24) were aged (4 degrees C) in the dark under vacuum for 0, 7, 14 and 21 days and the myofibrillar sub-proteome was extracted. Using 2-D DIGE minimal labelling, 35 spots that were differentially abundant between 0 and 21 days aged meat were detected. Of them, 24 were analysed by LC-MS/ MS, identifying a total of 29 equine proteins. These were structural and metabolic proteins, and among them, four (Actin, Troponin T and Myosin binding proteins 1 and 2) were selected for Western blot analysis, reporting changes in their abundance after 0, 7, 14 and 21 days of ageing. Results revealed that they should be further studied as potential protein biomarkers of horse meat tenderization. Additionally, several protein fragments increased after ageing, as was the case of glyceraldehyde-3-phosphate dehydrogenase. Fragments of this protein were present in four protein spots, and their study could be useful for monitoring horse meat tenderization. Significance: Tenderization during ageing has been widely studied in meat from several farm animal species; however, both research and standardized ageing practices are lacking for the particular case of horse meat. In this regard, this study presents novel proteomic findings related to post-mortem evolution of horse muscle pro-teins. Acquired knowledge would support the development and optimization of efficient ageing practices by horse meat industry.Department of Economic Development and Infrastructures of the Basque Government is acknowledged for the doctoral fellowship of L.R. Beldarrain. This work was funded by Lactiker Research Group (Basque Government IT944-16), project RTI2018-096162-R-C22 (Spanish Agencia Estatal de Investigación) and the Institute of Medical Biochemistry (University of Veterinary Medicine Vienna)

El màdel, una nova modalitat amb molt de futur

El màdel és una nova modalitat de pilota a mà, més concretament de pilota grossa, que va nàixer el 2013 a la localitat de Castelló de la Ribera, la Ribera Alta, davant l'evolució de la pilota valenciana i la necessitat d’aprofitar les instal·lacions disponibles per tot arreu, com les pistes de pàdel. Instal·lacions que mantenen clares semblances amb els trinquets de pilota grossa i que sols existeixen en 6 localitats on es pot practicar. L'afició al màdel ha crescut amb força els darrers anys gràcies a la bona acollida rebuda entre els aficionats a la pilota i altres nous practicants, que veuen en ell un valuós aparador per a conèixer el món de la pilota valenciana i les diferents modalitats que integra gràcies a la seua facilitat d’aprenentatge i adaptació cap a nous practicants. Des del 2013 els creadors del màdel, al costat de nombrosos clubs, escoles i associacions, han organitzat tornejos, tallers i partides d'exhibició en multitud de localitats de les diferents comarques valencianes, amb gran acceptació entre el públic de totes les edats i sexes. Alguns d'aquests tornejos s'han consolidat per a formar entre ells el I Circuit Intercomarcal de Màdel 2017, que ha tingut lloc en 7 localitats de 5 comarques valencianes diferents on han participat més de 160 jugadors i jugadores

The cell wall structure: developments in diagnosis and treatment of candidiasis

Candidiasis are among the fungal infections the most difficult to diagnose and treat. Research focused on specific fungal components which are absent in the host, such as the cell wall has lead to a better understanding of Candida albicans pathogenicity and clinical impact. The cell wall is responsible for antigenic expression and primary interaction with the host. It is composed mainly of β-glucans, chitin and mannoproteins, which account for the rigidity of the wall and for the fungal morphology. Of these components, mannoproteins might carry a “morphogenetic code” which might modulate the molecular architecture of the cell wall. The features of specific cell wall proteins as part of building blocks to form this structure is revised, and the usefulness of monoclonal antibodies obtained against cell wall components to study those processes, together with their clinical applicability, is discussed

Péptidos derivados de la troponina T generados en jamón curado

Comunicaciones a congreso