Analisi di settore: i servizi a valore aggiunto

Indice: Definizione del settore - Analisi dei fattori di diffusione dei vas - Contesto concorrenziale - Analisi della domand

Genome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strains

© 2022 Ricci et al. This article is available under a CreativeCommons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).Legionella pneumophila serogroup 1 (Lp1) sequence type (ST) 23 is one of the most commonly detected STs in Italy where it currently causes all investigated outbreaks. ST23 has caused both epidemic and sporadic cases between 1995 and 2018 and was analysed at genomic level and compared with ST23 isolated in other countries to determine possible similarities and differences. A core genome multi-locus sequence typing (cgMLST), based on a previously described set of 1,521 core genes, and single-nucleotide polymorphisms (SNPs) approaches were applied to an ST23 collection including genomes from Italy, France, Denmark and Scotland. DNAs were automatically extracted, libraries prepared using NextEra library kit and MiSeq sequencing performed. Overall, 63 among clinical and environmental Italian Lp1 isolates and a further seven and 11 ST23 from Denmark and Scotland, respectively, were sequenced, and pangenome analysed. Both cgMLST and SNPs analyses showed very few loci and SNP variations in ST23 genomes. All the ST23 causing outbreaks and sporadic cases in Italy and elsewhere, were phylogenetically related independent of year, town or country of isolation. Distances among the ST23s were further shortened when SNPs due to horizontal gene transfers were removed. The Lp1 ST23 isolated in Italy have kept their monophyletic origin, but they are phylogenetically close also to ST23 from other countries. The ST23 are quite widespread in Italy, and a thorough epidemiological investigation is compelled to determine sources of infection when this ST is identified in both LD sporadic cases and outbreaks.info:eu-repo/semantics/publishedVersio

Scrivere la storia fra Medioevo e prima età moderna

Seminario sulla scrittura cronachistica, notarile e cancelleresca, in latino e in volgare nell'Italia meridional

Battaglia nel Rinascimento meridionale. Moduli narrativi tra parole e immagini

Dal Trecento al Cinquecento, dagli estenuanti conflitti tra Angioini e Aragonesi alle guerre sanguinose tra Francia e Spagna, dagli scontri navali alle ribellioni baronali: 33 autori hanno unito le loro differenti competenze per indagare le modalità con le quali fu rappresentata la battaglia nell’Italia meridionale in un periodo cruciale per la storia politica e culturale europea. Al centro dell’indagine non sono tanto le battaglie in sé, pur qui criticamente ricostruite, quanto le differenti narrazioni di quelle battaglie, narrazioni in parole o in immagini. Il risultato della collaborazione tra studiosi di discipline diverse (storici, storici dell’arte, storici della letteratura, filologi italiani e latini) è una trattazione molto ricca ed articolata della letteratura, dell’arte, della storiografia del Mezzogiorno e sul Mezzogiorno tra XIV e XVI secolo. Descritte in miniature, in affreschi, negli arazzi, nel bronzo o nel marmo, nel latino forbito degli umanisti o nel volgare schietto degli ambasciatori, nei capolavori di Machiavelli o di Guicciardini, le battaglie diventano un’interessante chiave di lettura per una più approfondita conoscenza del Rinascimento meridionale

Nutrimento, Intrusione, Abuso: un continuum nella definizione dei limiti del corpo

Il manuale affronta il tema dei rapporti fra cibo e sessualità dal punto di vista biologico, psicologico e culturale. Dal punto di vista biologico, è innanzitutto necessario conoscere e comprendere: - i sofisticati sistemi neuroregolatori dell'attività sessuale, nell'uomo e nei modelli animali; - gli ormoni sessuali, il loro metabolismo e le modalità d'azione; - i meccanismi della fertilità e della procreazione, legate a equilibri nutrizionali oggi in grave pericolo. Dal punto di vista clinico, il fenomano più frequente è quello dell'anoressia sessuale, che ha basi biologiche e psichiche e che può giungere al rifiuto del partner

Drop-out rate in eating disorders: could it be a function of patient-therapist relationship?

OBJECTIVE: The factors influencing drop-out in eating disorders (ED) are still unclear. The aim of the present study was to determine whether compliance is strongly related to the patient-therapist relationship. MATERIALS AND METHODS: During 14 months all new patients affected by EDs referring to our Specialist Service Center, were assessed and followed up, they underwent EAT 40, EDI II, and computerized case history for ED. Moreover, we collected data from therapist using the GCI scale, and we recorded as the patient perceived his weight (PPW). RESULTS: We found that out of the 100 patients enrolled, 53 withdraw and when probability was predicted according to a digit model, it was not influenced by EDI-II subscales, age, sex, education, EAT-40 score, duration of the disorder and diagnosis while it significantly decreased when GCI scores increased and decreased when the PPW was altered. CONCLUSION: These results seem to confirm that some psychological factors related to patient-therapist relationship can play a key role for a stable and continuous therapeutic program

Differential Denaturation of Serum Proteome Reveals a Significant Amount of Hidden Information in Complex Mixtures of Proteins

Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected. In the present study we demonstrate that serum proteome denaturation is a key underestimated feature; in fact, a new differential denaturation protocol better discriminates serum proteins according to their electrophoretic mobility as compared to single-denaturation protocols. Sixty nine different denaturation treatments were tested and the 3 most discriminating ones were selected (TRIDENT analysis) and applied to human sera, showing a significant improvement of serum protein discrimination as confirmed by MALDITOF/ MS and LC-MS/MS identification, depending on the type of denaturation applied. Thereafter sera from mice and patients carrying cutaneous melanoma were analyzed through TRIDENT. Nine and 8 protein bands were found differentially expressed in mice and human melanoma sera, compared to healthy controls (p<0.05); three of them were found, for the first time, significantly modulated: alpha 2macroglobulin (down-regulated in melanoma, p<0.001), Apolipoprotein-E and Apolipoprotein-A1 (both up-regulated in melanoma, p<0.04), both in mice and humans. The modulation was confirmed by immunological methods. Other less abundant proteins (e.g. gelsolin) were found significantly modulated (p<0.05). Conclusions: i) serum proteome contains a large amount of information, still neglected, related to proteins folding; ii) a careful serum denaturation may significantly improve analytical procedures involving complex protein mixtures; iii) serum differential denaturation protocol highlights interesting proteomic differences between cancer and healthy sera

Differential Denaturation of Serum Proteome Reveals a Significant Amount of Hidden Information in Complex Mixtures of Proteins

<div><p>Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected. In the present study we demonstrate that serum proteome denaturation is a key underestimated feature; in fact, a new differential denaturation protocol better discriminates serum proteins according to their electrophoretic mobility as compared to single-denaturation protocols. Sixty nine different denaturation treatments were tested and the 3 most discriminating ones were selected (TRIDENT analysis) and applied to human sera, showing a significant improvement of serum protein discrimination as confirmed by MALDI-TOF/MS and LC-MS/MS identification, depending on the type of denaturation applied. Thereafter sera from mice and patients carrying cutaneous melanoma were analyzed through TRIDENT. Nine and 8 protein bands were found differentially expressed in mice and human melanoma sera, compared to healthy controls (p<0.05); three of them were found, for the first time, significantly modulated: α2macroglobulin (down-regulated in melanoma, p<0.001), Apolipoprotein-E and Apolipoprotein-A1 (both up-regulated in melanoma, p<0.04), both in mice and humans. The modulation was confirmed by immunological methods. Other less abundant proteins (e.g. gelsolin) were found significantly modulated (p<0.05).</p> <p>Conclusions: i) serum proteome contains a large amount of information, still neglected, related to proteins folding; ii) a careful serum denaturation may significantly improve analytical procedures involving complex protein mixtures; iii) serum differential denaturation protocol highlights interesting proteomic differences between cancer and healthy sera.</p> </div

MALDI-TOF/MS analysis of differentially expressed bands in melanoma patients.

<p>MALDI-TOF/MS analysis of differentially expressed bands in melanoma patients.</p><p>Differentially expressed bands by TRIDENT-SDS-PAGE identified by MALDI-TOF/MS in human sera from cancer patients compared to the healthy controls. P value means the significance between densitometry of control (Ctrl) bands <i>vs</i> melanoma bands (Mel), whose modulation is reported as State change. Prot ID describes the name of the protein and AC# the accession number.</p