Analysis of cysteine-containing proteins using precolumn derivatization with N-(2-ferroceneethyl)maleimide and liquid chromatography/electrochemistry/mass spectrometry

N-(2-Ferroceneethyl)maleimide (FEM) is introduced as an electroactive derivatizing agent for thiol functionalities in proteins. Using appropriate reaction conditions, the derivatization is completed within five minutes and no unspecific labeling of free amino functions is observed. Liquid chromatography/electrochemistry/mass spectrometry was used to detect the reaction products. The reagent is a useful tool for determining the number of free thiol groups or the total number of free and disulfide-bound thiol groups in proteins. The electrochemical cell provides additional information, because the increase in mass spectrometric response upon electrochemical oxidation of the neutral ferrocene to the charged ferrocinium groups is monitored. The method was successfully applied to the analysis of native proteins and their tryptic digests

May Bells Ringing : Maiglockchens Lauten

https://digitalcommons.library.umaine.edu/mmb-ps/1248/thumbnail.jp

Non-Covalent Functionalization of Graphene Films for Uniform Nanoparticle Deposition via Atoic Layer Deposition

Graphene functionalized with platinum (Pt) and palladium (Pd) has proven to be highly effective as a hydrogen sensor. Deposition methods such as Atomic layer deposition (ALD) can be further enhanced by pretreating the graphene with a non-covalent surfactant prior to nanoparticle deposition. In this study, graphene-based sensing devices will be fabricated by ALD deposition. The graphene will be non-covalently functionalized using sodium dodecyl sulfate (SDS) anionic surfactant prior to ALD deposition. The aim of this study is to test the deposition pattern achieved by varying the amount of time that graphene is treated with the SDS surfactant. Initially, ALD deposition of Zinc-Oxide (ZnO) will be performed and the resulting patterns will be analyzed using SEM imagining. The resulting patterns will then be used to aid in the deposition of Pt and Pd onto graphene using ALD in future work

Different deformations of proton and neutron distributions in nuclei

Different collective deformation coordinates for neutrons and protons are introduced to allow for both stretching and γ transitions consistent with experiments. The rotational actinide nuclei 234-238U and 232Th are successfully analyzed in this model. NUCLEAR STRUCTURE 232Th, 234-238U calculated B (E2) values, collective model

Ancestor Worship and State Rituals in Contemporary China: Fading Boundaries between Religious and Secular

The paper argues that the distinction between religious and secular realms of society is not as clear-cut in modern societies as it appears in theories of functional and institutional differentiation. The data used are mainly from China with a short excursion to the United States. The starting point is ancestor worship, which is a central element of traditional Chinese religion. The significance of ancestor worship in Chinese history and culture is briefly explained to illustrate on the one hand its central importance as a ritual practice and on the other hand the ambiguities of interpretation. On this basis, some theoretical considerations about the existence of ancestors are presented. This is followed by a report on contemporary temple festivals focusing on the worship of Fuxi, a mythic figure considered to be the first ancestor of the Chinese people. The next step is the description of official state rituals devoted to the worship of the very same mythological hero in contemporary China. Against this backdrop, the last part of the paper discusses the theoretical questions of classification and distinguishing between the religious and the secular

Test of the proximity theorem for deformed nuclei

We compare a proximity-type potential for two interacting nuclei with the double-folding method. Both spherical and deformed systems are considered. Special "orientation windows" are found for two deformed nuclei giving rise to nuclear cohesion. If the same nucleon-nucleon interaction is utilized, the proximity and the double-folding potentials agree fairly well for a spherical + deformed system. However, deviations are found in the case of two deformed nuclei

Generation of soap films with instantaneously adsorbed surfactants: concentration-dependent film thinning

International audienceIn this theoretical work, we predict the steady state thickness of soap films pulled from a bath of surfactants. Assuming simplified thermodynamical properties for the surfactants, we compute the interfacial stresses by taking explicitly into account surfactant convection along the film. We make no assumption on interfacial rheology: the rigidification of the interfaces results entirely from confinement and depletion effects. Two main approximations are used: the concentration of surfactants is supposed homogeneous within the thickness of the film, and at equilibrium with the adsorbed layer. With these hypotheses, we show that the thickness of the film follows Frankel's law at low capillary numbers, and that deviations occur at higher pulling velocities. We study the dependence of the film thickness with the characteristics of the surfactant used and especially with its initial concentration, and we show that our predictions are compatible with available data by Saulnier and coworkers