422 research outputs found
UPPER BODY SEGMENT LENGTHS AS A PROPORTION OF HEIGHT IN CHILDREN
Most research studies in biomechanics directly measure body segment lengths via anthropometry or digitization of joint markers. There are circumstances in which estimating segment lengths in relation to height is desirable, such as in biomechanical modelling or in the classroom. One commonly used model for this purpose is that by Drillis and Contini (1966; cited in Winter, 2005). One problem with this model is that the initial data was derived from adults, and thus has potentially limited applicability to the study of biomechanics in children. The purpose of the present study was to compare actual selected upper body segment lengths measured via anthropometry to those predicted by Drillis and Contini and also to derive regression equations for those segment lengths based on height and age (separately for males and females)
Evidence That the P\u3csub\u3ei\u3c/sub\u3e Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle
Nitrogenase reduction of dinitrogen (N2) to ammonia (NH3) involves a sequence of events that occur upon the transient association of the reduced Fe protein containing two ATP molecules with the MoFe protein that includes electron transfer, ATP hydrolysis, Pi release, and dissociation of the oxidized, ADP-containing Fe protein from the reduced MoFe protein. Numerous kinetic studies using the nonphysiological electron donor dithionite have suggested that the rate-limiting step in this reaction cycle is the dissociation of the Fe protein from the MoFe protein. Here, we have established the rate constants for each of the key steps in the catalytic cycle using the physiological reductant flavodoxin protein in its hydroquinone state. The findings indicate that with this reductant, the rate-limiting step in the reaction cycle is not protein–protein dissociation or reduction of the oxidized Fe protein, but rather events associated with the Pi release step. Further, it is demonstrated that (i) Fe protein transfers only one electron to MoFe protein in each Fe protein cycle coupled with hydrolysis of two ATP molecules, (ii) the oxidized Fe protein is not reduced when bound to MoFe protein, and (iii) the Fe protein interacts with flavodoxin using the same binding interface that is used with the MoFe protein. These findings allow a revision of the rate-limiting step in the nitrogenase Fe protein cycle
Sequential and Differential Interaction of Assembly Factors During Nitrogenase MoFe Protein Maturation
Nitrogenases reduce atmospheric nitrogen, yielding the basic inorganic molecule ammonia. The nitrogenase MoFe protein contains two cofactors, a [7Fe-9S-Mo-C-homocitrate] active-site species, designated FeMo-cofactor, and a [8Fe-7S] electron-transfer mediator called P-cluster. Both cofactors are essential for molybdenum-dependent nitrogenase catalysis in the nitrogen-fixing bacterium Azotobacter vinelandii. We show here that three proteins, NafH, NifW, and NifZ, copurify with MoFe protein produced by an A. vinelandii strain deficient in both FeMo-cofactor formation and P-cluster maturation. In contrast, two different proteins, NifY and NafY, copurified with MoFe protein deficient only in FeMo-cofactor formation. We refer to proteins associated with immature MoFe protein in the following as “assembly factors.” Copurifications of such assembly factors with MoFe protein produced in different genetic backgrounds revealed their sequential and differential interactions with MoFe protein during the maturation process. We found that these interactions occur in the order NafH, NifW, NifZ, and NafY/NifY. Interactions of NafH, NifW, and NifZ with immature forms of MoFe protein preceded completion of P-cluster maturation, whereas interaction of NafY/NifY preceded FeMo-cofactor insertion. Because each assembly factor could independently bind an immature form of MoFe protein, we propose that subpopulations of MoFe protein–assembly factor complexes represent MoFe protein captured at different stages of a sequential maturation process. This suggestion was supported by separate isolation of three such complexes, MoFe protein–NafY, MoFe protein–NifY, and MoFe protein–NifW. We conclude that factors involved in MoFe protein maturation sequentially bind and dissociate in a dynamic process involving several MoFe protein conformational states
A new analytical approach for the velocity field in rolling processes and its application in through-thickness texture prediction
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Observations of the atmosphere and surface state over Terra Nova Bay, Antarctica, using unmanned aerial systems
In September 2012 five Aerosonde unmanned aircraft were used to make measurements of the atmospheric state over the Terra Nova Bay polynya, Antarctica, to explore the details of air–sea ice–ocean coupling. A total of 14 flights were completed in September 2012. Ten of the flight missions consisted of two unmanned aerial systems (UAS) sampling the atmosphere over Terra Nova Bay on 5 different days, with one UAS focusing on the downwind evolution of the air mass and a second UAS flying transects roughly perpendicular to the low-level winds. The data from these coordinated UAS flights provide a comprehensive three-dimensional data set of the atmospheric state (air temperature, humidity, pressure, and wind) and surface skin temperature over Terra Nova Bay. The remaining UAS flights during the September 2012 field campaign included two local flights near McMurdo Station for flight testing, a single UAS flight to Terra Nova Bay, and a single UAS flight over the Ross Ice Shelf and Ross Sea polynya. A data set containing the atmospheric and surface data as well as operational aircraft data have been submitted to the United States Antarctic Program Data Coordination Center (USAP-DCC)
Beyond fossil fuel–driven nitrogen transformations
How much carbon does it take to make nitric acid? The counterintuitive answer nowadays is quite a lot. Nitric acid is manufactured by ammonia oxidation, and all the hydrogen to make ammonia via the Haber-Bosch process comes from methane. That's without even accounting for the fossil fuels burned to power the process. Chen et al. review research prospects for more sustainable routes to nitrogen commodity chemicals, considering developments in enzymatic, homogeneous, and heterogeneous catalysis, as well as electrochemical, photochemical, and plasma-based approaches
Frontiers, Opportunities, and Challenges in Biochemical and Chemical Catalysis of CO_2 Fixation
Two major energy-related problems confront the world in the
next 50 years. First, increased worldwide competition for
gradually depleting fossil fuel reserves (derived from past
photosynthesis) will lead to higher costs, both monetarily and politically. Second, atmospheric CO_2 levels are at their highest recorded level since records began. Further increases are predicted to produce large and uncontrollable impacts on the world climate. These projected impacts extend beyond climate to ocean acidification, because the ocean is a major sink for atmospheric CO2.1 Providing a future energy supply that is secure and CO_2-neutral will require switching to nonfossil energy sources such as wind, solar, nuclear, and geothermal energy and developing methods for transforming the energy produced by these new sources into forms that can be stored, transported, and used upon demand
Guided play and free play in an enriched environment: impact on motor development
The purpose of this study was to investigate the effects of guided play and free play in an enriched environment
intervention programs using motor skill development in kindergarten children. Seventy-one children attending
kindergarten classes were assigned to two experimental groups and one control group. Participants performed the Test
of Gross Motor Development-2 before and after the intervention period. Results revealed that both boys and girls in the
guided play group showed motor skill improvement, whereas no changes were observed in motor development in the
boys and girls assigned to the free play in enriched environment group, nor in those in the control group. These findings
indicate that the teacher’s role in the guided play intervention was crucial to help preschool children to improve their
performance.CIEC – Research Centre on Child Studies, UM (FCT R&D 317
Dispersed free phytosterols as structuring agents in lipid systems with reduced saturated fat
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