Application of FMEA method for assessment of reliability of structures

In the last years several methods have been proposed, aimed to explore the theme of degradation and reliability of building components, with the main purpose of preventing performance deficits in general, and especially from sudden and unforeseen faults. This topic is an element of strong competitiveness in the world of professionals and business, and fits with the trend clearly demonstrated by the regulatory framework, particularly in the field of structures. One of the methods that can be used to assess the suitability of a component to its performance specifications, and which belongs to the field of Risk Management, is FMEA, (Failure Modes and Effects Analysis). This methodology analyzes a project from the point of view of its reliability before it is carried out and realized.To allow this verification the components are firstly verified in the form of drawings, technical specifications, flow-charts, information, standards, etc. that are all the elements that make up the documentation of the work to be performed and the knowledge that is available on the subject. There have been many applications of FMEA on building components, and it is of particular interest - considering the recrudescence of seismic events of the last decade, but also the emergence of structural defects, even of a certain gravity – for the use in the field of structures. This is of a considerable relevance in a period when there are issues about the possibility of adaptation of the real estate to earthquakes, but it also raises the argument of the modalities and the criteria leading the inevitable reconstruction of what no longer exists. In this article an example of application to a reinforced concrete structure is shown, from which it is possible to notice the most critical items and the modalities for preventing negative events

Performance characterization of the concrete subjected to fire in terms of sustainability

The research, in a context of a sustainability of the building process, that should consider adequately the problem of building waste disposal, has the objective of determining the performance characteristics of concrete under the effect of fire, in order to evaluate the possibility of reutilization, and in positive case, what could the best uses be, considering the current regulations. The experimentation, carried out on cubic samples with a side of 15 cm (according to UNI EN 12390, using aggregates with Dmax= 30 mm), with Portland cement of mixture 42,5 in two types (one with Pozzolana CEM II/B-P 42.5 and one with limestone CEM II/A-LL 42.5), with class of resistance C25/30, is based on the following parameters: increase of temperature, nature of fire, heating speed, time of exposition. The tests that have been carried out were both destructive (sclerometric, ultrasounds, Sonreb) and non-destructive (crushing and thermogravimetric), and the experimental results obtained also show that: - the concretes present a remaining mechanical resistance that is lower than 50% of the initial one, after they have been subjected to the full cycle of temperature increase; - between 200°C and 600°C, there is a significant decomposition of the binding component (hydrated cement), while that of limestone is predictable for temperatures higher than 700°C; - the phenomenon of the “explosive spalling” – verified on two samples – has far more devastating consequences than the simple decay of the mechanical characteristics as, considered in a structural element, it causes a reduction of the resisting section, with a consequential increase of tensions in the remaining part, and direct exposition of the reinforcement without protection, situations which can cause the structural collapse of the element. Some uses in the field of transport have been hypothesized, also basing on the indications of the current regulations and on the detected statistics of the current uses

SPATIALLY EXPLICIT MODEL OF AREAS BETWEEN SUITABLE BLACK BEAR HABITAT IN EAST TEXAS AND BLACK BEAR POPULATIONS IN LOUISIANA, ARKANSAS, AND OKLAHOMA

Although black bears (Ursus americanus, Ursus americanus luteolus) were once found throughout the south-central United States, unregulated harvest and habitat loss resulted in severe range retractions and by the beginning of the twentieth century populations in Oklahoma, Louisiana, Texas and Arkansas were nearing extirpation. In response to these losses, translocation programs were initiated in Arkansas (1958-1968 & 2000-2006) and Louisiana (1964-1967 & 2001-2009). These programs successfully restored bears to portions of Louisiana and Arkansas, and, as populations in Arkansas began dispersing, to Oklahoma. In contrast, east Texas remains unoccupied despite the existence of suitable habitat in the region. To facilitate the establishment of a breeding population in east Texas, I sought to identify suitable habitat which bears could use for dispersal between known bear locations in Louisiana, Arkansas and Oklahoma and the east Texas recovery units. I utilized Maxent, a machine learning software, to model habitat suitability in this region. I collected known black bear presence locations (n=18,241) from state agencies in Louisiana, Oklahoma, Arkansas and east Texas and filtered them to reduce spatial autocorrelation (n=664). I also collected spatial data sets based on known black bear ecology to serve as environmental predictor variables. The model was developed at 30-m resolution and encompassed 417,076 km 2. The final model was selected to minimize model over-fitting while maintaining a high test Area Under the Receiver Operating Curve (AUC TEST)score. For final model interpretation and analysis, I used the 10th percentile training threshold available in Maxent which excludes the lowest 10% of predicted presence suitability scores from the binary predictive map, thus resulting in a more conservative predictive map. The final 10th percentile model predicted 43.7% of the pixels in the study area as suitable and 53.7 % percent of the pixels identified as potential recovery units by Kaminski et al. (2013, 2014) as suitable. To focus management efforts, I identified three movement zones with a high proportion of suitable habitat within which connectivity analyses were performed. Suitable patches greater than or equal to 12 km2 were classified within ArcGIS as stepping stone patches. Buffers of 3,500 m were generated around these patches to determine the level of functional connectivity in each zone. The final Maxent model confirmed that suitable bear habitat exists between source populations and the east Texas recovery units. The importance of percent of mast producing forest, percentage of cultivated crops and percentage of protected lands reflect what is known about basic bear biology and ecology. Furthermore, 153 stepping stone patches were identified within the movement zones, demonstrating that there is a reasonable chance of bears naturally dispersing to east Texas using the habitat identified in this study. Thus, protection of existing bear habitat and the stepping stone patches identified in this study should be a priority for managers seeking to facilitate natural bear recolonization of east Texas

MULTICRITERIA ANALYSIS FOR CHOOSING MAINTENANCE STRATEGIES

The life cycle of building components can be managed according to different maintenance strategies, which mainly differ in performance and economic terms. What is the most convenient among the possible scenarios? It has been shown in the past that the typology of maintenance interventions and the consequent periodicity are closely related to performance decay, and can lead to choices that, however, generally concern the purely economic sphere. In this sense, it seems interesting to know the modalities of the performance decay, which may allow – even though for many components the "measurement" of its values is problematic - the construction of performance / time curves. This result was possible, in other experiments conducted in the past, for one of the components to be considered most critical for the whole building (the plaster), thanks to a study that sampled 53 buildings with homogeneous characteristics (both from the technological point of view and from the era of realization), observed within 20 years. This paper highlights that the only economic evaluation is not enough to identify the ideal solution, because – inter alia - there is a more suitable solution depending on the context framework in which the decision maker is operating. Commitment, budget, component typology, time span to consider, are the main factors influencing the choice, not ignoring design issues. A Topsis multicriteria analysis is proposed, the results of which are an interesting starting point for define maintenance plans characterized by greater reliability, not only technical but also economical

LCC analysis for glued laminated timber components exposed in external

The aim of the experimentation is to characterize the performances of glued laminated timber components exposed to atmospheric agents, in the light of the concept of Life Cycle Cost, particularly emphasized in the Italian new legislation on public works. In fact, it could be seen that the design of the construction details and the initial characteristics of glue laminated timber components, require a particular attention to avoid errors that may undermine the possibility to perform the structural functions for which, above all, this material it is dedicated. The experimentation consisted of a series of tests aimed to identify the performance of glued laminated timber beams taken in site, after a work executed some years ago on a theatre built in Roman era, and to compare them with similarly manufactured new elements. The tests performed were thermography, ultrasound, Wood-Pecker penetrometer, mechanical characterization. The results will be used to begin setting limits and conditions for outdoor use of glue laminated timber, and assume a reliable life cycle, and consequently identify the most appropriate maintenance strategy for the LCC

Healthcare associated pathogens in a changing world

In developed countries about 10% of the hospitalizations are complicated by a healthcare-associated infection [1]. Up to 75% of these infections are due to multidrug-resistant organisms (MDROs) [1]. Antimicrobial resistant bacterial infections are associated to higher morbidity, mortality and healthcare costs than those caused by susceptible organisms [1]. The findings of the point prevalence survey in European acute care hospitals published in 2013 by the European Centre for Disease Control and Prevention (ECDC) show large variations between countries and between different regions of the same country, with Italy being allocated within the high-endemic areas for both MRSA and MDROs [2]. Despite antimicrobial resistance affects most bacterial species, MDR Gram negatives represent the most serious threat. In a few years Enterobacteriaceae, mainly Escherichia coli and Klebsiella pneumoniae, have evolved from extended spectrum β-lactamase (ESBL) producing to carbapanem-resistant organisms [3]. Simultaneously, Acinetobacter baumannii has quickly become extremely or pan-drug resistant [4]. Carbapenem resistant Gram negatives heavily impact on clinical outcomes with mortality rates significantly higher than the susceptible strains of the same species [1]. Of further concern, very few antimicrobial agents are available for an effective treatment of these infections and new agents active against these organisms are not currently in development. Many intertwining factors are driving these epidemiological changes, involving patients, healthcare delivery systems, infection control practices and, most important, misuse and inappropriate use of antibiotics in all healthcare facilities, in community and in animal husbandry. In particular, the transition of the healthcare delivery systems from a hospital-centered model to a healthcare facility network has gradually blurred the borders between hospital and community and the patients’ travel within this network has critically contributed to disseminate MDROs [5]. As a consequence, antimicrobial resistance is now as common, if not more so, in post-acute clinical facilities, such as long term care settings and nursing homes [5]. The “revolving door” is the very efficacious image used as the paradygm of the spreading routes of organisms with hospital and community reservoirs, as E. coli or MRSA. The revolving door, indeed, enlightens how the colonized patients entering back and forth several healthcare settings drive the amplification of the antibiotic resistance [6]. Stringent infection control and prevention practices and wise use of antibiotics are unanimously agreed as the key actions to fight MDROs. Of course, we need new antibiotics, but first we have to learn how to protect them from a precipitous erosion of their effectiveness

Spatial Autocorrelation and Pseudoreplication in Fire Ecology

Fire ecologists face many challenges regarding the statistical analyses of their studies. Hurlbert (1984) brought the problem of pseudoreplication to the scientific community’s attention in the mid 1980’s. Now, there is a new issue in the form of spatial autocorrelation. Spatial autocorrelation, if present, violates the traditional statistical assumption of observational independence. What, if anything, can the fire ecology community do about this new problem? An understanding of spatial autocorrelation, and knowledge of available methods used to reduce the effect of spatial autocorrelation and pseudoreplication will greatly assist fire ecology researchers

Satisfaction with chronic obstructive pulmonary disease treatment: results from a multicenter, observational study

Background: Understanding the level of patients' satisfaction with treatment and its determinants have the potential to impact therapeutic management and clinical outcome in chronic conditions such as chronic obstructive pulmonary disease (COPD). Methods: A national, multicenter, longitudinal, observational study of COPD from 20 Italian pulmonary centers to explore patients' satisfaction to treatment [assessed by the Treatment Satisfaction Questionnaire, 9 items (TSQM-9)] and association with clinical parameters [including dyspnea score, COPD Assessment Test (CAT) score, exacerbation rate], adherence to treatment [Morisky Medication-Taking Adherence Scale (MMAS-4)], illness perception [evaluated by Brief Illness Perception Questionnaire (B-IPQ)] in a 1-year follow up. Results: A total of 401 COPD patients were enrolled [69.4% group B Global Initiative for COPD (GOLD), considering 366 patients with available GOLD 2017 classification at enrollment]. At enrollment, satisfaction with treatment was moderate, being TSQM-9 mean scores for effectiveness 64.2 [95% confidence interval (CI) 62.5-65.9], for convenience 75.8 (95% CI 74.2-77.3), and for global satisfaction 65.7 (95% CI 64.0-67.4). Global satisfaction was negatively associated with disease perception (beta = -0.4709, p < 0.0001), and grade of dyspnea (beta = -4.2564, p = 0.009). Satisfaction with treatment was lower in patients with poor compared with optimal adherence to treatment (beta = -4.5608, p = 0.002). Changes in inhalation regimens during follow up did not modify the satisfaction with treatment. Conclusions: The results of this real-life study showed that the patients' satisfaction with treatments is only moderate in COPD. A high grade of patients' satisfaction is associated mainly with a low perception of the disease, high adherence to treatment and lower level of dyspnea

Deregulation of cancer-stem-cell-associated miRNAs in tissues and sera of colorectal cancer patients

Colorectal cancer (CRC) is a deadly tumour in Western countries characterized by high cellular/molecular heterogeneity. Cancer stem cells (CSC) act in cancer recurrence, drug-resistance and in metastatic epithelial-to-mesenchymal transition. microRNAs (miRNAs) contribute to cancer is increasing, and miRNA roles in CSC phenotype and fate and their utility as CRC biomarkers have also been reported. Here, we investigated miR-21, miR-221, miR-18a, miR-210, miR-31, miR-34a, miR-10b and miR-16 expression in experimental ALDH+ and CD44+/CD326+ colorectal CSCs obtained from the human CRC cell lines HCT-116, HT-29 and T-84. Then, we moved our analysis in cancer tissue (CT), healthy tissue (HT) and serum (S) of adult CRC patients (n=12), determining relationships with clinical parameters (age, sex, metastasis, biochemical serum markers). Specific miRNA patterns were evident in vitro (normal, monolayers and CSCs) and in patients' samples stratified by TNM stage (LOW vs HIGH) or metastasis (Met vs no-Met). miR-21, miR-210, miR-34a upregulation ad miR-16 dowregulation associated with the CSCs phenotype. miR-31b robustly overexpressed in monolayers and CSCs, and in CT ad S of HIGH grade and Met patients, suggesting a role as marker of CRC progression and metastasis. miR-18a upregulated in all cancer models and associated to CSC phenotype, and to metastasis and age in patients. miR-10b downregulated in CT and S of LOW/HIGH grade and no-Met patients. Our results identify miRNAs useful as colorectal CSC biomarker and that miR-21, miR-210, miR-10b and miR-31b are promising markers of CRC. A specific role of miR-18a as metastatic CRC serum biomarker in adult patients was also highlighted

The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer

Colorectal cancer (CRC) is the third most frequent cancer worldwide and the second cause of cancer deaths. Increasing evidences supports the idea that the poor prognosis of patients is related to the presence of cancer stem cells (CSCs), a cell population able to drive cancer recurrence and metastasis. The deregulation of microRNAs (miRNAs) plays a role in the formation of CSC. We investigated the role of hsa-miR-486-5p (miR-486-5p) in CRC, CSCs, and metastasis, in order to reach a better understanding of the biomolecular and epigenetic mechanisms mir-486-5p-related. The expression of miR-486-5p was investigated in three di erent matrices from CRC patients and controls and in CSCs obtained from the CRC cell lines HCT-116, HT-29, and T-84. In the human study, miR-486-5p was up-regulated in serum and stool of CRC patients in comparison with healthy controls but down-regulated in tumor tissue when compared with normal mucosa. miR-486-5p was also down-regulated in the sera of metastatic patients. In vitro, miR-486-5p was down-regulated in CSC models and it induced an inhibitory e ect on stem factors and oncogenes in the main pathways of CSCs. Our results provide a step forward in understanding the role of mir-486-5p in CRC and CSC, and suggest that further studies are needed to investigate its diagnostic and prognostic power, possibly in combination with other biomarkers.Instituto de Salud Carlos III PIE16-00045 DTS19/00145Junta de AndalucíaEuropean Union (EU) SOMM17/6109/UGR (UCE-PP2017-3)Chair "Doctors Galera-Requena in cancer stem cell research" CMC-CTS963Fondazione Banco di Sardegn