Collagen-induced arthritis as an animal model of rheumatoid cachexia

Background Rheumatoid arthritis is characterized by chronic polyarticular synovitis and presents systemic changes that impact quality of life, such as impaired muscle function, seen in up to 66% of the patients. This can progress to severely debilitating state known as rheumatoid cachexia—without loss of fat mass and body weight—for which there is little consensus in terms of diagnosis or treatment. This study aims to evaluate whether the collagen-induced arthritis (CIA) animal model also develops clinical and functional features characteristic of rheumatoid cachexia. Methods Male DBA1/J mice were randomly divided into 2 groups: healthy animals (CO, n = 11) and CIA animals (n = 13). The clinical score and edema size, animal weight and food intake, free exploratory locomotion, grip strength, and endurance exercise performance were tested 0, 18, 35, 45, 55, and 65 days after disease induction. After euthanasia, several organs, visceral and brown fat, and muscles were dissected and weighed. Muscles were used to assess myofiber diameter. Ankle joint was used to assess arthritis severity by histological score. Statistical analysis were performed using one-way and two-way analyses of variance followed by Tukey’s and Bonferroni’s test or t-test of Pearson and statistical difference were assumed for a P value under 0.05. Background Rheumatoid arthritis is characterized by chronic polyarticular synovitis and presents systemic changes that impact quality of life, such as impaired muscle function, seen in up to 66% of the patients. This can progress to severely debilitating state known as rheumatoid cachexia—without loss of fat mass and body weight—for which there is little consensus in terms of diagnosis or treatment. This study aims to evaluate whether the collagen-induced arthritis (CIA) animal model also develops clinical and functional features characteristic of rheumatoid cachexia. Methods Male DBA1/J mice were randomly divided into 2 groups: healthy animals (CO, n = 11) and CIA animals (n = 13). The clinical score and edema size, animal weight and food intake, free exploratory locomotion, grip strength, and endurance exercise performance were tested 0, 18, 35, 45, 55, and 65 days after disease induction. After euthanasia, several organs, visceral and brown fat, and muscles were dissected and weighed. Muscles were used to assess myofiber diameter. Ankle joint was used to assess arthritis severity by histological score. Statistical analysis were performed using one-way and two-way analyses of variance followed by Tukey’s and Bonferroni’s test or t-test of Pearson and statistical difference were assumed for a P value under 0.0

Instrumentos de triagem odontológica para idosos institucionalizados: uma revisão de escopo : Dental screening instruments for institutionalized old people: a scope review

Introduction: Population aging in Brazil and in the world is a reality and the institutionalization of the olds is still a common practice, although debatable. The oral health condition of institutionalized elderly people is precarious for many reasons, with many treatment needs and difficulty in accessing dental treatment. A great demand for treatment and reduced service capacity justifies the use of dental screening tools to prioritize care and classify old people by risk scale. Objective: This study aims to review the literature in order to identify the different dental screening tools used to organize and prioritize dental care for institutionalized elderly people. Methodology: After defining the research theme, the descriptors in the DeCS/MeSH databases were selected for the literature search. Studies published in the last 20 years and that presented instruments for dental screening in institutionalized elderly were included in the research. Articles that presented only dental assessment instruments without screening and literature reviews were excluded. Results: After removing duplicates, and titles, abstracts and full texts reading, 10 articles remained. The articles analyzed were published between 2005 and 2023 and come from 9 countries. The 10 analyzed articles presented a total of 8 screening instruments for use in institutionalized elderly, with the Oral Health Assessment Tool (OHAT), present in 6 of the 10 articles. Conclusion: After analyzing the literature, it can be seen that the OHAT is the most appropriate instrument for dental screening of institutionalized elderly, mainly because it does not depend on the cognitive function and cooperation of the patient, it is simple and applicable by caregivers or other health professionals.Introdução: O envelhecimento populacional é uma realidade e a institucionalização de idosos é uma prática comum. A condição de saúde bucal dos idosos institucionalizados é precária por inúmeros motivos, com muitas necessidades de tratamento e dificuldade de acesso. A grande demanda e a reduzida capacidade de atendimento justificam o uso de instrumentos de triagem odontológica para classificação de risco. Objetivo: Identificar os instrumentos de triagem odontológica utilizados para priorizar o atendimento odontológico de idosos institucionalizados presentes na literatura. Metodologia: Revisão de escopo conduzida, utilizando a extensão do checklist PRISMA-ScR, nas seguintes bases de dados: MEDLINE via Pubmed, Scopus, Embase, Web of Science. Incluíram-se estudos que responderam à pergunta de pesquisa publicados nos últimos 20 anos. Excluíram-se artigos que apresentaram instrumentos de avaliação sem triagem e revisões de literatura. Resultados: Após a remoção das duplicatas, exclusão por títulos, resumos e leitura de textos completos, restaram 10 artigos. Os textos analisados, publicados entre os anos de 2005 e 2023, são provenientes de 9 países e apresentaram um total de 6 instrumentos de triagem para uso em idosos institucionalizados, tendo o Oral Health Assessment Tool (OHAT) presente em 6 dos 10 artigos. Conclusão: Após análise da literatura, pode-se observar que o OHAT é o instrumento mais adequado para triagem odontológica de idosos institucionalizado, principalmente por não depender da função cognitiva e cooperação do paciente, ser simples e aplicável por cuidadores ou outros profissionais da saúde

Metabolomic biomarker candidates for skeletal muscle loss in the collagen-induced arthritis (CIA) model

There is no consensus for diagnosis or treatment of RA muscle loss. We aimed to investigate metabolites in arthritic mice urine as biomarkers of muscle loss. DBA1/J mice comprised collagen-induced arthritis (CIA) and control (CO) groups. Urine samples were collected at 0, 18, 35, 45, 55, and 65 days of disease and subjected to nuclear magnetic resonance spectroscopy. Metabolites were identified using Chenomx and Birmingham Metabolite libraries. The statistical model used principal component analysis, partial least-squares discriminant analysis, and partial least-squares regression analysis. Linear regression and Fisher’s exact test via the MetaboAnalyst website were performed (VIP-score). Nearly 100 identified metabolites had CIA vs. CO and disease time-dependent differences (p < 0.05). Twenty-eight metabolites were muscle-associated: carnosine (VIPs 2.8 × 102) and succinyl acetone (VIPs 1.0 × 10) showed high importance in CIA vs. CO models at day 65; CIA pair analysis showed histidine (VIPs 1.2 × 102) days 55 vs. 65, histamine (VIPs 1.1 × 102) days 55 vs. 65, and L-methionine (VIPs 1.1 × 102) days 0 vs. 18. Carnosine was fatigue- (0.039) related, creatine was food intake- (−0.177) and body weight- (−0.039) related, and both metabolites were clinical score- (0.093; 0.050) and paw edema- (0.125; 0.026) related. Therefore, muscle metabolic alterations were detected in arthritic mice urine, enabling further validation in RA patient’s urine, targeting prognosis, diagnosis, and monitoring of RA-mediated muscle loss

Genetic Associations and Differential mRNA Expression Levels of Host Genes Suggest a Viral Trigger for Endemic Pemphigus Foliaceus

The long search for the environmental trigger of the endemic pemphigus foliaceus (EPF, fogo selvagem) has not yet resulted in any tangible findings. Here, we searched for genetic asso ciations and the differential expression of host genes involved in early viral infections and innate antiviral defense. Genetic variants could alter the structure, expression sites, or levels of the gene products, impacting their functions. By analyzing 3063 variants of 166 candidate genes in 227 EPF patients and 194 controls, we found 12 variants within 11 genes associated with differential suscepti bility (p < 0.005) to EPF. The products of genes TRIM5, TPCN2, EIF4E, EIF4E3, NUP37, NUP50, NUP88, TPR, USP15, IRF8, and JAK1 are involved in different mechanisms of viral control, for example, the regulation of viral entry into the host cell or recognition of viral nucleic acids and proteins. Only two of nine variants were also associated in an independent German cohort of sporadic PF (75 patients, 150 controls), aligning with our hypothesis that antiviral host genes play a major role in EPF due to a specific virus–human interaction in the endemic region. Moreover, CCL5, P4HB, and APOBEC3G mRNA levels were increased (p < 0.001) in CD4+ T lymphocytes of EPF patients. Because there is limited or no evidence that these genes are involved in autoimmunity, their crucial role in antiviral responses and the associations that we observed support the hypothesis of a viral trigger for EPF, presumably a still unnoticed flavivirus. This work opens new frontiers in searching for the trigger of EPF, with the potential to advance translational research that aims for disease prevention and treatment

Household slow sand filters in intermittent and continuous flows to treat water containing low mineral ion concentrations and Bisphenol A

Household slow sand filter (HSSF) has been used as an alternative to drinking water treatment in rural communities worldwide; however, its performance to treat influent water with quality similar to rainwater still needs further studies. Rainwater presents low pH and slight mineral ion concentrations, an aspect that can modify the filter media and consequently the HSSF efficiency. Furthermore, house roofs used in rainwater harvesting can be made of plastic. Therefore, it can introduce chemicals such as Bisphenol A (BPA) in the water. In this context, two pilot-scale HSSFs operated in continuous and intermittent flows were evaluated to treat water containing BPA and low mineral ion concentrations in order to assess the filter performance. Filter media leaching was noticed in the trials; thus, filter media and construction material selection must be carefully evaluated to eliminate risks of pollutant occurrence in drinking water. Operational differences between continuous and intermittent flows influenced the HSSF efficiency for BPA and DOC removals; even so, the filters' performance was low probably due to the slow schmutzdecke development. According to tracer test results, HSSF can be classified as a plug flow reactor and strategies to improve its hydraulic performance are not required

Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses

The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species