cGAT. Konventionen für das computergestützte Transkribieren in Anlehnung an das Gesprächsanalytische Transkriptionssystem 2 (GAT2)

Mit dem cGAT-Handbuch stellt das FOLK-Projekt eine Richtlinie für das computergestützte Transkribieren nach GAT 2 zur Verfügung. Das Handbuch wurde anhand der Transkriptionspraxis in FOLK entwickelt und enthält eine Vielzahl von authentischen Beispielen, die mit dem zugehörigen Audio auch über die Datenbank für Gesprochenes Deutsch (DGD) abgerufen werden können

A Taxonomy of Violations in Digital Asset Markets

Numerous frauds, market manipulations and other violations have recently shaken investor confidence in digital asset markets and digital assets themselves. Yet, investor confidence and market integrity are key requirements for the continued success of crypto and other digital assets. In order to facilitate the integrity of digital asset markets and avoid integrity incidents in the future, a systematic overview of violations and their main characteristics is needed to develop appropriate countermeasures. Therefore, we develop a taxonomy of violations in digital asset markets and evaluate the taxonomy based on real-world cases. Our results show that many types of market manipulation in traditional financial markets can also be observed in digital asset markets. However, there are new and additional violations in digital asset markets. We also find that many violations depend on specific capabilities of the violator, certain trading conditions, and asset-specific characteristics

A new framework for urban ecology: an integration of proximate and ultimate responses to anthropogenic change

As urban areas continue to grow, understanding how species respond and adapt to urban habitats is becoming increasingly important. Knowledge of the mechanisms behind observed phenotypic changes of urban-dwelling animals will enable us to better evaluate the impact of urbanization on current and future generations of wildlife and predict how animals respond to novel environments. Recently, urban ecology has emerged not only as a means of understanding organismal adaptation but also as a framework for exploring mechanisms mediating evolutionary phenomena. Here, we have identified four important research topics that will advance the field of urban ecology and shed light on the proximate and ultimate causes of the phenotypic differences commonly seen among species and populations that vary in their responses to urbanization. First, we address the ecological and socio-economic factors that characterize cities, how they might interact with each other, and how they affect urban species. Second, we ask which are the proximate mechanisms underlying the emergence over time of novel traits in urban organisms, focusing on developmental effects. Third, we emphasize the importance of understanding the ultimate causations that link phenotypic shifts to function. This question highlights the need to quantify the strength and direction of selection that urban individuals are exposed to, and whether the phenotypic shifts associated with life in the city are adaptive. Lastly, we stress the need to translate how individual-level responses scale up to population dynamics. Understanding the mechanistic underpinnings of variation among populations and species in their responses to urbanization will unravel species resilience to environmental perturbation, which will facilitate predictive models for sustainability and development of green cities that maintain or even increase urban biodiversity and wildlife health and wellbeing

Construction competencies and building quality : Case study results

Zugleich gedruckt erschienen im Universitätsverlag der Technischen Universität Berlin, 2013. - ISBN 978-3-7983-2506-7, ISSN 2193-6099This volume of the Young Cities Research Paper series examines the interdependency between the energy efficiency of buildings, the quality of construction, and the level of workmanship. The results are based on multiple case studies at construction sites in the Tehran-Karaj region, the work of experts, interviews with Hashtgerd residents, and analyses of Iranian earthquake studies. The research activities took place from 2009 to 2012. The main part of this book consists of a classification of damage cases in concrete work, energy efficient construction, welding, site arrangement and installation work. These fields were identified as the most important ones. The book has been conceived as a combination academic study and manual to evaluate the quality of workmanship and results at construction sites. It is not intended to substitute technical books, but rather to identify problematic situations, clarify possible subsequent damages and reconstruction options, and to concisely describe proper execution

Template-Assisted Enzymatic Synthesis of Oligopeptides from a Polylactide Chain

Peptides are often attached to polymer materials, as bioactive components, for the control of interactions between the material and its surrounding proteins and cells. However, synthesizing peptides and attaching them to polymers can be challenging and laborious. Herein, we describe the grafting of oligopeptides to an aliphatic polyester, using a one-step chemo-enzymatic synthesis with papain as the biocatalySt. To enable enzyme-mediated functionalization of the polyester, ethyl hept-6-enoylalaninate (grafter) was synthesized and attached to polylactide chains using thiol-ene click reactions. The oligopeptides were grafted onto the polylactide chains using two different synthetic routes: the grafting from strategy, in which the grafter was attached to the polyester prior to oligopeptide synthesis, or the grafting to strategy, in which oligopeptides were synthesized on the grafter first, then attached to the polymer chain. The final products were analyzed and their structures were confirmed using nuclear magnetic resonance (NMR). The peptide attachment was evaluated using size exclusion chromatography (SEC), contact angle measurement and energy-dispersive X-ray spectroscopy scanning electron microscopy (EDS-SEM). Furthermore, the mechanistic aspects of the synthesis of the oligopeptides on the grafter were studied using molecular dynamics (MD) simulations. The simulation revealed that hydrogen bonding (between the P1 amide nitrogen of the grafter backbone and the carbonyl oxygen of D158 in the papain) maintain the grafter in a productive conformation to stabilize the transition state of nitrogen inversion, a key step of the biocatalytic mechanism. Apart from being biologically relevant, both experimental and computational results suggest that the designed grafter is a good template for initiating chemo-enzymatic synthesis. The results also showed that the grafting to strategy was more successful compared to the grafting from strategy. Overall, a successful synthesis of predefined peptide functionalized polylactide was prepared, where the oligopeptides were grafted in an easy, time efficient, and environmentally friendly way. QC 2017121

Influence of time, toothpaste and saliva in the retention of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes

Objectives: The intraoral transmission of cariogenic and periodontopathogenic species seems to be facilitated by contaminated toothbrushes and other oral hygiene devices. The aim of this investigation was to analyze the in vitro retention and survival rate of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes. The impacts of human saliva and antimicrobial toothpaste on these parameters were further evaluated. Material and Methods: Part I: Four toothbrushes (Colgate 360°, Curaprox CS5460 ultra soft, elmex InterX, Trisa Flexible Head3) were contaminated by S. mutans DSM 20523 or S. sanguinis DSM 20068 suspensions for three minutes. Bacteria were removed from the toothbrushes after either three minutes (T0) or 24 hours (T24) of dry storage and grown on Columbia blood agar plates for the quantification of colony-forming units (CFUs). Part II: The effects of saliva from a caries-active or a caries-inactive person and of toothpaste containing 0.12% chlorhexidine digluconate were also tested. Results: Part I: After three minutes of dry storage, approximately one percent of the bacteria were still detectable on the toothbrushes. After 24 hours, S. sanguinis exhibited a more pronounced decrease in viable cell numbers compared with S. mutans but the differences were not significant (Kruskal-Wallis test, p>;0.05). Part II: The addition of human saliva from a caries-active or caries-inactive person slightly increased the retention of both streptococcal species at T0. The use of toothpaste had no influence on the amount of viable streptococci at T0, but it reduced the microbial load after 24 hours of storage. There were only slight nonsignificant differences (p>;0.05) between the four toothbrushes. Conclusions: In vitro bacterial retention and survival of S. sanguinis and S. mutans on different toothbrushes occurred. Within the limitations of this study, the use of human saliva or an antimicrobial toothpaste did not lead to significant differences in the microbial load on toothbrushes