Large quantum dots with small oscillator strength

We have measured the oscillator strength and quantum efficiency of excitons confined in large InGaAs quantum dots by recording the spontaneous emission decay rate while systematically varying the distance between the quantum dots and a semiconductor-air interface. The size of the quantum dots is measured by in-plane transmission electron microscopy and we find average in-plane diameters of 40 nm. We have calculated the oscillator strength of excitons of that size and predict a very large oscillator strength due to Coulomb effects. This is in stark contrast to the measured oscillator strength, which turns out to be much below the upper limit imposed by the strong confinement model. We attribute these findings to exciton localization in local potential minima arising from alloy intermixing inside the quantum dots.Comment: 4 pages, 3 figures, submitte

Analyzing marker substances for Complex Regional Pain Syndrome (CRPS)

Weniger als 5% der Patienten entwickeln Komplex-Regionales Schmerzsyndrom (CRPS) nach einem Trauma, insbesondere nach Frakturen. Es ist ein schmerzhaftes Syndrom, dass durch eine Vielzahl von klinischen Merkmalen gekennzeichnet ist. Es kann chronisch werden, wenn es nicht in den ersten Monaten kuriert wird. Wahrscheinlich spielen mehrere pathophysiologische Mechanismen eine Rolle in CRPS. Es wird vermutet, dass Neuropeptide und anti-inflammatorische Lipid-Mediatoren involviert sind. In dieser Arbeit wurden diese Moleküle in Hautbiopsien und Serum mit dem Ziel der Korrelation ihrer Konzentration mit klinischen Parametern mittels Massenspektrometrie (MS) untersucht. Hochauflösende und insbesondere NanoMS identifizierte Peptide und Fettsäuren im niederen fmol-Bereich. Die Methodik zeigte aber auch wenig Toleranz gegenüber dem chemischen Untergrund, so dass vornehmlich die robustere Kapillarchromatography eingesetzt wurde. Die Serum-Proteaseaktivität mit einem Fokus auf Angiotensin-konvertierendem Enzym (ACE) wurde untersucht. Bradykinin (BK) wurde zügig zu BK1-8 und BK1-5 abgebaut. Niedrigere BK1-5 Levels waren in Übereinstimmung mit der Hypothese verringerter ACE-Aktivität in CRPS.Less than 5% of patients develop Complex Regional Pain Syndrome (CRPS) after trauma, mostly after fractures. It is a painful syndrome characterized by a variety of clinical features including classical signs of inflammation and it can become chronical if not cured in the first few months. Likely, a number of pathophysiological mechanisms play a role in CRPS. The involvement of neuropeptides and anti-inflammatory lipid mediators has been suggested. Here, mass spectrometry (MS) was used to investigate these molecules in skin biopsies and serum with the aim of correlating their concentration with clinical parameters. High-end and in particular nanoscale MS identified peptides as well as fatty acids at the low fmol level. However, it also showed little tolerance for the chemical background so that a more robust capillary chromatography approach was preferentially used. Serum protease activity with a focus on angiotensin converting enzyme (ACE) was studied. Bradykinin (BK) was rapidly degraded to BK1-8 and BK1-5. The formation of lower BK1-5 levels was indicated in agreement with the hypothesis of reduced ACE-activity in CRPS

Composite Weak Bosons: a Lattice Analysis

We present a lattice analysis of a confining Yang-Mills theory without Goldstone boson. We have analytically investigated the model by a strong coupling expansion and by an intensive lattice Monte Carlo simulation using standard lattice QCD methods. We show that this theory is an interesting candidate for describing weak bosons as composite particles.Comment: Late

Construction of the Soudan 2 detector

Progress in the construction of the Soudan 2 nucleon decay detector which is being built at the Soudan iron mine in Minnesota is discussed. The expected event rate and characteristics of low energy neutrino events, muon events, multiple muon events, and other cosmic ray phenomena are discussed

Patterns of Sympathetic Responses Induced by Different Stress Tasks

Stress tasks are used to induce sympathetic nervous system (SNS) arousal. However, the efficacy and the patterns of SNS activation have not been systematically compared between different tasks

Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield

Open Access Article; Published online: 17 Jan 2020Cassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouse‐grown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement

Composite Weak Bosons: a Lattice Monte Carlo Analysis

We present a lattice Monte Carlo simulation for the evaluation of the spectrum of a confining Yang-Mills theory without Goldstone boson. We show that this theory is a very good candidate for describing composite weak bosons. In order to perform the spectrum analysis we have used standard lattice QCD Monte Carlo methods. We have also developed an efficient method to evaluate the mass of the pseudoscalar isosinglet which is present in our theory.Comment: Late

A bispecific diabody directed against prostate-specific membrane antigen and CD3 induces T-cell mediated lysis of prostate cancer cells

BACKGROUND: Although cancer of the prostate is one of the most commonly diagnosed cancers in men, no curative treatment currently exists after its progression beyond resectable boundaries. Therefore, new agents for targeted treatment strategies are needed. Cross-linking of tumor antigens with T-cell associated antigens by bispecific monoclonal antibodies have been shown to increase antigen-specific cytotoxicity in T-cells. Since the prostate-specific membrane antigen (PSMA) represents an excellent tumor target, immunotherapy with bispecific diabodies could be a promising novel treatment option for prostate cancer. METHODS: A heterodimeric diabody specific for human PSMA and the T-cell antigen CD3 was constructed from the DNA of anti-CD3 and anti-PSMA single chain Fv fragments (scFv). It was expressed in E. coli using a vector containing a bicistronic operon for co-secretion of the hybrid scFv V_HCD3-V_LPSMA and V_HPSMA-V_LCD3. The resulting PSMAxCD3 diabody was purified from the periplasmic extract by immobilized metal affinity chromatography (IMAC). The binding properties were tested on PSMA-expressing prostate cancer cells and PSMA-negative cell lines as well as on Jurkat cells by flow cytometry. For in vitro functional analysis, a cell viability test (WST) was used. For in vivo evaluation the diabody was applied together with human peripheral blood lymphocytes (PBL) in a C4-2 xenograft-SCID mouse model. RESULTS: By Blue Native gel electrophoresis, it could be shown that the PSMAxCD3 diabody is mainly a tetramer. Specific binding both to CD3-expressing Jurkat cells and PSMA-expressing C4-2 cells was shown by flow cytometry. In vitro, the diabody proved to be a potent agent for retargeting PBL to lyze C4-2 prostate cancer cells. Treatment of SCID mice inoculated with C4-2 tumor xenografts with the diabody and PBL efficiently inhibited tumor growth. CONCLUSIONS: The PSMAxCD3 diabody bears the potential for facilitating immunotherapy of prostate cancer and for the elimination of minimal residual disease