Improved production of lutein and β-carotene by thermal and light intensity upshifts in the marine microalga Tetraselmis sp. CTP4

The industrial microalga Tetraselmis sp. CTP4 is a promising candidate for aquaculture feed, novel food, cosmeceutical and nutraceutical due to its balanced biochemical profile. To further upgrade its biomass value, carotenogenesis was investigated by testing four environmental factors, namely temperature, light intensity, salinity and nutrient availability over different growth stages. The most important factor for carotenoid induction in this species is a sufficient supply of nitrates leading to an exponential growth of the cells. Furthermore, high temperatures of over 30 degrees C compared to lower temperatures (10 and 20 degrees C) induced the accumulation of carotenoids in this species. Remarkably, the two different branches of carotenoid synthesis were regulated depending on different light intensities. Contents of beta-carotene were 3-fold higher under low light intensities (33 mu mol m(-2) s(-1)) while lutein contents increased 1.5-fold under higher light intensities (170 and 280 mu mol m(-2) s(-1)). Nevertheless, highest contents of carotenoids (8.48 +/- 0.47 mg g(-1) DW) were found upon a thermal upshift from 20 degrees C to 35 degrees C after only two days at a light intensity of 170 mu mol m(-2) s(-1). Under these conditions, high contents of both lutein and beta-carotene were reached accounting for 3.17 +/- 0.18 and 3.21 +/- 0.18 mg g(-1) DW, respectively. This study indicates that Tetraselmis sp. CTP4 could be a sustainable source of lutein and beta-carotene at locations where a robust, euryhaline, thermotolerant microalgal strain is required.Funding Agency Portuguese Foundation for Science and Technology UID/Multi/04326/2019 SFRH/BD/115325/2016 SFRH/BD/140143/2018 SFRH/BD/105541/2014 0055 ALGARED+ 05 INTERREG V-A -Espana Portugal project national Portuguese funding PPBI-POCI-01-0145-FEDER-22122 Nord Universityinfo:eu-repo/semantics/publishedVersio

THE PRE-EXHAUSTION METHOD SEEMS INEFFECTIVE TO INCREASE ELECTROMYOGRAPHIC ACTIVITY

The purpose of this study was to investigate the effects of the pre-exhaustion method on the electromyographic activity (temporal and spectral domain) at different intervals of a set of resistance training. Twenty adults with little or no experience in strength training performed two sets of the seated row exercise at 70%1RM until muscular failure, in a randomized order. Surface electrodes were placed over the latissimus dorsi, teres major, biceps brachii, and posterior deltoid muscles of the dominant side. Results showed that the pre-exhaustion did not increase EMG activity of all muscle analyzed at any intervals of a set. Furthermore, the EMG median frequency decreased in teres major and posterior deltoid muscles during initial and intermediate repetitions. Results suggest that the pre-exhaustion induces more fatigue when compared to traditional training

MOTIVAÇÃO E CONFIABILIDADE HUMANA: UMA ANÁLISE DA PERCEPÇÃO DO INDIVÍDUO

Este artigo apresenta uma análise da influência da motivação sobre a percepção de risco e a prática de ato inseguro do empregado na execução de suas atividades. Concluiu-se que o empregado afirma estar motivado, embora com lacunas que, no entanto, não aumentam os erros de execução. Há consciência do erro, porém isso não resulta em prevenção

PODER RELATIVO DO LUCRO CONTÁBIL E DO FLUXO DE CAIXA DAS OPERAÇÕES PARA PREVER FLUXOS DE CAIXA FUTUROS: UM ESTUDO EMPÍRICO NO BRASIL

Este artigo avalia a eficácia da previsão de fluxos futuros, para um e dois anos à frente, de quatro medidas de desempenho da empresa, sendo duas de fluxo de caixa e duas de lucro, a partir de medidas ex post do Fluxo de Caixa das Operações (FCO), isolado e em conjunto com a variação das alocações (accruals) de curto prazo (?AcBcpB), e do Lucro Líquido contábil (LL). A metodologia de previsão é do tipo externo à amostra. São feitas várias previsões anuais e bianuais transversais para as 92 empresas que compõem a amostra, ao longo dos anos de 1996 a 2004. Os desvios (erros) médios absolutos de previsão, a Mediana dos erros e as correlações médias entre os valores reais e projetados das variáveis são apurados e comparados entre si para fins de verificação da eficácia de previsão dos fluxos futuros de cada um dos três modelos. Os resultados revelam que: (1) LL é superior ao FCO para prever o Lucro Líquido do ano seguinte, mas, ao contrário, FCO é superior ao LL para prever o Lucro Líquido dois anos à frente; (2) a combinação das alocações de curto prazo (?AcBcpB) com o FCO não melhora as previsões que são feitas apenas com o FCO, indicando que o Fluxo de Caixa das Operações é mais eficaz para prever fluxos futuros de caixa e de lucro do que o capital circulante líquido; e (3) o Lucro Operacional (LO), como é definido na legislação societária brasileira, é de difícil previsão

Being and becoming a teacher: Professional Wellbeing Amidst COVID-19 Pandemic

Covid-19 has a significant impact on higher education worldwide, not just in the Philippines. This study described the professional quality of life of the college teachers in terms of compassion satisfaction and compassion fatigue. This study ascertained the relationship of socio demographic profile of the college teachers and their professional quality of life. This study used a quantitative-descriptive method of research. Convenience sampling was used in this study. A total of 76 college instructors were surveyed (44 from public schools and 32 from private schools). Based on the results of the study, it is concluded that the college teachers have high compassion satisfaction for getting satisfaction from being able to teach people, being proud of what they can do to teach, and being happy to their chosen profession. These college teachers have exposure work related secondary traumatic stress on a moderate degree level by having a feeling of worn out because of their work as a teacher and feeling overwhelmed because of their teaching load and other work-related activities. However, the secondary traumatic stress of college teachers did not result to having high level of burnout, though college teachers still experience burnout but in low degree level only

The Challenges to Make Decent Work a Reality for Domestic Workers in Brazil

The present paper deals with the problematic of labor and social security rights of domestic workers in Brazil and around the world highlighting the importance of more inclusive and fair public policies and private actions for this category of workers The research analyzed official documents and statistics as well as bibliographic research from authors who discuss the subjec

Biogeochemical and microbial variation across 5500 km of Antarctic surface sediment implicates organic matter as a driver of Benthic Community Structure.

This is the final version of the article. Available from Frontiers Media via the DOI in this record.Western Antarctica, one of the fastest warming locations on Earth, is a unique environment that is underexplored with regards to biodiversity. Although pelagic microbial communities in the Southern Ocean and coastal Antarctic waters have been well-studied, there are fewer investigations of benthic communities and most have a focused geographic range. We sampled surface sediment from 24 sites across a 5500 km region of Western Antarctica (covering the Ross Sea to the Weddell Sea) to examine relationships between microbial communities and sediment geochemistry. Sequencing of the 16S and 18S rRNA genes showed microbial communities in sediments from the Antarctic Peninsula (AP) and Western Antarctica (WA), including the Ross, Amundsen, and Bellingshausen Seas, could be distinguished by correlations with organic matter concentrations and stable isotope fractionation (total organic carbon; TOC, total nitrogen; TN, and δ(13)C). Overall, samples from the AP were higher in nutrient content (TOC, TN, and NH4 (+)) and communities in these samples had higher relative abundances of operational taxonomic units (OTUs) classified as the diatom, Chaetoceros, a marine cercozoan, and four OTUs classified as Flammeovirgaceae or Flavobacteria. As these OTUs were strongly correlated with TOC, the data suggests the diatoms could be a source of organic matter and the Bacteroidetes and cercozoan are grazers that consume the organic matter. Additionally, samples from WA have lower nutrients and were dominated by Thaumarchaeota, which could be related to their known ability to thrive as lithotrophs. This study documents the largest analysis of benthic microbial communities to date in the Southern Ocean, representing almost half the continental shoreline of Antarctica, and documents trophic interactions and coupling of pelagic and benthic communities. Our results indicate potential modifications in carbon sequestration processes related to change in community composition, identifying a prospective mechanism that links climate change to carbon availability.Funds through NSF Antarctic Program: AM (CMU: Award Number 1043670), KH, and SS (AU Award Number: 1043745) and from Central Michigan University Faculty Research and Creative Endeavors (FRCE) Committee and College of Science and Technolog

Psychophysiological response of military pilots in different combat flight maneuvers in a flight simulator

Objective: To analyze the psychophysiological response during takeoff, landing, air-air attack and air-ground attack maneuvers. Methods: A total of 11 expert pilots (age=33.36 (5.37)) from the Spanish Air Force participated in this cross-sectional study. Participants had to complete in a flight simulator the following missions: 1) takeoff; 2) Air-air attack; 3) Air-Ground attack, and 4) Landing. The electroencephalographic activity (EEG) and heart rate variability (HRV) were collected during all these maneuvers. Results: Significant higher values of theta (during takeoff, air-air attack and air-ground attack) EEG power spectrum were obtained when compared to baseline. Significant difference in the P3 scalp location was observed between landing and takeoff maneuvers in the beta EEG power spectrum. Furthermore, significant lower values of HRV were obtained during takeoff, landing, air-air attack and air-ground attack when compared to baseline values. Also, landing showed a higher sympathetic response when compared to takeoff maneuver. Conclusion: Takeoff, landing, air-air attack or air-ground attack maneuvers performed in a flight simulator produced significant changes in the electroencephalographic activity and autonomic modulation of professional pilots. Beta EEG power spectrum modifications suggest that landing maneuvers induced more attentional resources than takeoff. In the same line, a reduced HRV during landing was obtained when compared to takeoff. These results should be considered to training purposes

Anaerobic digestion of road-side-green-cuttings as a poten-tial phytoremediator with different lead concentrations

The utilization of roadside-green-cuttings (grass) for anaerobic digestion increases provides an additional possible source of organic waste for use as a renewable energy source. Grass can be used as a substrate to increase biogas yield. Nevertheless, the anaerobic digestion of this kind of waste can be limited due to the fact that it could be contaminated with heavy metals, in particular from traffic emissions and industrial activity. For this reason the biogas production of grass from a busy road was assessed. Samples of roadside-grass were washed with an organosulphide, which is used for the removal of heavy metals from wastewater. A comparison of the anaerobic digestion of washed and unwashed roadside grass was performed. Results showed that the anaerobic digestion of the unwashed grass was much more effective than the washed grass. A second experiment was carried out and co-fermentation of manure and farm-grass was prepared for anaerobic digestion. Lead was added in the concentrations 500, 1000 and 2000 mg Pb2+/kg. The results showed that the higher the lead concentration, the lower the inhibition of the biogas yield. The grass could be acting as phytoremediator for high lead concentrations. The grass could contain organic compounds, which can as-similate heavy metals

AS TECNOLOGIAS DIGITAIS DA INFORMAÇÃO E COMUNICAÇÃO NO TRABALHO DOCENTE: PRÁTICAS NO ENSINO MÉDIO DE UMA ESCOLA PÚBLICA

O presente trabalho buscou investigar as ações desenvolvidas por quatro professores do Ensino Médio de uma escola pública de Campo Grande. O referido estudo teve como objetivo descrever, analisar e avaliar os procedimentos do trabalho docente associados às tecnologias digitais da informação e comunicação (TDICs)