Variacion estacional de las asociaciones de copepodos en una transecta de la plataforma argentina

Three faunistic areas were identified along a transect off the Argentine coast (38º11'S 55º11'W to 39º20'S 55º07W) based on the distribution of copepod taxa: coastal, transitional midshelf and outershelf-oceanic in summer, autumn and winter 1987. The copepods formed different assemblages depending on the season, with a certain overlapping among some of them. Calanus propinquus, Clausocalanus brevipes and Oithona atlântica a group identified in March, appeared in other assemblages during the studied period. Calanus australis, Calanoides carinatus and Centropages brachiatus, a group also formed in summer, continued together in the following two seasons in other groups. The oceanic species were only found in March and May, excepting Clausocalanus laticeps which was present in July (winter). The number of recorded taxa decreased from 19 to 12 along the studied period

Clustered Intracellular Salmonella enterica Serovar Typhimurium blocks host cell cytokinesis

Several bacterial pathogens and viruses interfere with the cell cycle of their host cells to enhance virulence. This is especially apparent in bacteria that colonize the gut epithelium, where inhibition of the cell cycle of infected cells enhances the intestinal colonization. We found that intracellular Salmonella enterica serovar Typhimurium induced the binucleation of a large proportion of epithelial cells by 14 h postinvasion and that the effect was dependent on an intact Salmonella pathogenicity island 2 (SPI-2) type 3 secretion system. The SPI-2 effectors SseF and SseG were required to induce binucleation. SseF and SseG are known to maintain microcolonies of Salmonella-containing vacuoles close to the microtubule organizing center of infected epithelial cells. During host cell division, these clustered microcolonies prevented the correct localization of members of the chromosomal passenger complex and mitotic kinesin-like protein 1 and consequently prevented cytokinesis. Tetraploidy, arising from a cytokinesis defect, is known to have a deleterious effect on subsequent cell divisions, resulting in either chromosomal instabilities or cell cycle arrest. In infected mice, proliferation of small intestinal epithelial cells was compromised in an SseF/SseG-dependent manner, suggesting that cytokinesis failure caused by S. Typhimurium delays epithelial cell turnover in the intestine

Quantification and localization of formylated phloroglucinol compounds (FPCs) in eucalyptus species

The Eucalyptus genus is a hyper-diverse group of long-lived trees from the Myrtaceae family, consisting of more than 700 species. Eucalyptus are widely distributed across their native Australian landscape and are the most widely planted hardwood forest trees in the world. The ecological and economic success of Eucalyptus trees is due, in part, to their ability to produce a plethora of specialized metabolites, which moderate abiotic and biotic interactions. Formylated phloroglucinol compounds (FPCs) are an important class of specialized metabolites in the Myrtaceae family, particularly abundant in Eucalyptus. FPCs are mono- to tetra-formylated phloroglucinol based derivatives, often with an attached terpene moiety. These compounds provide chemical defense against herbivory and display various bioactivities of pharmaceutical relevance. Despite their ecological and economic importance, and continued improvements into analytical techniques, FPCs have proved challenging to study. Here we present a simple and reliable method for FPCs extraction, identification and quantification by UHPLC-DAD-ESI-Q-TOF-MS/MS. The method was applied to leaf, flower bud, and flower samples of nine different eucalypt species, using a small amount of plant material. Authentic analytical standards were used to provide high resolution mass spectra and fragmentation patterns. A robust method provides opportunities for future investigations into the identification and quantification of FPCs in complex biological samples with high confidence. Furthermore, we present for the first time the tissue-based localization of FPCs in stem, leaf, and flower bud of Eucalyptus species measured by mass spectrometry imaging, providing important information for biosynthetic pathway discovery studies and for understanding the role of those compounds in planta

Corrigendum: Quantification and localization of Formylated Phloroglucinol Compounds (FPCs) in Eucalyptus Species

Error in Figure/Table In the original article, there was a mistake in Figure 4 and Supplementary Table S2 as published. There was an error during the FPCs quantification process, whereby the ratio of injection volume between sample and standard was accidentally inverted. This error has resulted in the overestimation of FPCs concentration reported, but does not alter the biological significance of the results. The corrected Figure 4 appears below, and Supplementary Table S2 has been replaced in the original article. Furthermore, in the original article, there was an error in the results section where the number of total FPCs for different tissues of two species are cited. A correction has been made to the Results section, sub-section Detection and Quantification of FPCs, paragraph four: “From all species analyzed, E. camphora and E. globulus had the highest concentration of total FPCs in leaves, with 65 and 41mg g−1 DW, respectively (Figure 4, Supplementary Table S2). Eucalyptus camphora also had high concentration of FPCs in flower buds and flowers, with 13 and 12mg g−1 DW, respectively. Interestingly, three Eucalyptus species showed a tendency to accumulate more FPCs in flowers compared to the leaves. Eucalyptus leucoxylon, E. sideroxylon, and E. viminalis contained ~40, 5, and 3 times more total FPCs in the flowers compared to leaves, respectively Figure 4, Supplementary Table S2. Eucalyptus yarraensis presented very low amounts of FPCs in leaves and flower buds, and it is the only species that does not contain any sideroxylonals. Eucalyptus cladocalyx and C. ficifolia did not show any traces of this class of specialized metabolites in the tissues analyzed.

Stem cell therapy as adjunctive in the management of anemia and thrombocytopenia partially responsive to corticosteroid in a dog treated with Phenobarbital - case report

ABSTRACT This report describes a dog with anemia and ITP, associated with phenobarbital, treated with prednisolone with a good response after association with three intravenous transplantations of 2.5x106 allogenic Mesenchymal Stem-Cells. After the first transplantation, the number of platelets had an improvement, but started to decrease after three weeks. However, the dose of corticosteroid could be reduced. After the second and third administrations, new increases were observed. The platelet count varied from 102,000 to 365,000 until the end of follow-up and without increase of prednisolone doses. Although phenobarbital therapy has been associated with thrombocytopenia, it was not possible to change the anti-convulsant therapy. The response to corticosteroid-based therapy was partial. Cell therapy was followed by an improvement in platelet count with no observed side effects and therefore could be considered safe. Effects on platelet count were transitory, but until the end of follow-up, no severe thrombocytopenia was observed. Based on the observation that platelet numbers dropped a few weeks after cell therapy, more applications would be beneficial in order to maintain higher platelet counts

Heritability of fasting glucose levels in a young genetically isolated population

Aims/hypothesis: The heritability of fasting glucose levels in Northern European populations has been examined previously in twins and samples of small pedigrees. In this study the heritability of fasting plasma glucose (FPG) was estimated in participants in the Erasmus Rucphen Family study, who were members of a single pedigree from a young genetic isolate. We also studied the relationship between FPG and components of the metabolic syndrome. Methods: FPG, lipid, blood pressure and body composition measurements were completed for 852 participants without diabetic medication. The most significant predictors of FPG were used as covariates in heritability estimation. The sibship effect, which is a composite of genetic dominance and shared early-life environmental effects, was included as a random effect. Results: The age- and sex-adjusted heritability of log normal-transformed FPG was 36.6%. When further adjusted for metabolic risk factors, namely body composition parameters, systolic blood pressure, triglycerides and cholesterol:HDL ratio, the heritability estimate rose to 42.8%. After adjustment for the sibship effect, the additive component of heritability was estimated to be 28.3% (age-and sex-adjusted) and 24.9% (full model). Conclusions/ interpretation: Genes control a significant proportion of the variance in FPG levels. Adjustment for other metabolic risk factors did not substantially change the heritability estimate, which suggests that a large part of the variance in FPG levels is due to genes that act through pathways that are independent of those controlling body composition, blood pressure and lipid levels

Análise geoambiental do riacho Santa Bárbara Zona urbana – Parnarama-Maranhão

Análise geoambiental do riacho Santa BárbaraZona urbana – Parnarama-Maranhã

Waist circumference as a vital sign in clinical practice: a Consensus Statement from the IAS and ICCR Working Group on Visceral Obesity

Despite decades of unequivocal evidence that waist circumference provides both independent and additive information to BMI for predicting morbidity and risk of death, this measurement is not routinely obtained in clinical practice. This Consensus Statement proposes that measurements of waist circumference afford practitioners with an important opportunity to improve the management and health of patients. We argue that BMI alone is not sufficient to properly assess or manage the cardiometabolic risk associated with increased adiposity in adults and provide a thorough review of the evidence that will empower health practitioners and professional societies to routinely include waist circumference in the evaluation and management of patients with overweight or obesity. We recommend that decreases in waist circumference are a critically important treatment target for reducing adverse health risks for both men and women. Moreover, we describe evidence that clinically relevant reductions in waist circumference can be achieved by routine, moderate-intensity exercise and/or dietary interventions. We identify gaps in the knowledge, including the refinement of waist circumference threshold values for a given BMI category, to optimize obesity risk stratification across age, sex and ethnicity. We recommend that health professionals are trained to properly perform this simple measurement and consider it as an important 'vital sign' in clinical practice

Distribution and abundance of cephalopods in UK waters: long-term trends and environmental relationships

As part of a project which aimed to evaluate the feasibility of developing indicators of marine ecosystem status based on cephalopods, we analysed spatiotemporal variation in abundance,, and environmental relationships, using trawl survey catch data for cephalopods in UK waters (1980-2013) from Cefas and Marine Scotland Science databases. These data presented some challenges, notably the use of several different trawl gears, variable tow durations, and varying levels of taxonomic resolution. Accounting for gear type and tow duration, data were analysed separately for each cephalopod family and season to account for different phases of the life cycles being present at different times of year. The families investigated were Loliginidae, Octopodidae, Ommastrephidade, Sepiidae and Sepiolidae. A GAM framework was used to summarise spatiotemporal variation in abundance at family level and the relationships of spatial and long-term temporal variation with environmental variables, including depth, substrate (available for inshore waters) and several oceanographic variables (e.g., SST, chl signals), also considering fishing pressure. Long-term trends for each family varied between areas and seasons, although this may reflect the presence of several species within families. In Scotland, where Loligo vulgaris is rare and L. forbesii is normally distinguished from Alloteuthis spp., survey data suggested a peak in abundance of this species around 1990 and a generally increasing trend since the mid-1990s. Spatial patterns in distribution in all families were related to both physiographic and oceanographic features. As expected substrate type had most effect on those families in which eggs are attached to objects on the seabed