Construction of a tungsten coil atomic emission spectrometer (WCAES)

It is here discussed the development of a low cost analytical instrument with capacity for metals determination using atomic emission measurements in an electrothermal atomization system with a tungsten coil atomizer. The main goal was to show a new frontier for using this atomizer and to demonstrate that the simple instrumental arrangement here proposed has potential for portability and for solving analytical tasks related to metals determination. Atomic emission of calcium was selected for the adjustment of instrumental parameters and to evaluate the main characteristics of the lab-built instrument. Cobalt was determined in medicines and one alloy to demonstrate its feasibility.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPES

Relating LANDSAT ETM+ and forest inventory data for mapping successional stages in a tropical wet forestRelacionando LANDSAT ETM+ e dados de inventário florestal para mapeamento estádios sucessionais em uma floresta tropical úmida

AbstractIn this study, we test whether an existing classification technique based on the integration of LANDSAT ETM+ and forest inventory data enables detailed characterization of successional stages in a tropical wet forest site. The specific objectives were: (1) to map forest age classes across the La Selva Biological Station in Costa Rica; and (2) to quantify uncertainties in the proposed approach in relation to field data and existing vegetation maps. Although significant relationships between vegetation hight entropy (a surrogate for forest age) and ETM+ data were detected, the classification scheme tested in this study was not suitable for characterizing spatial variation in age at La Selva, as evidenced by the error matrix and the low Kappa coefficient (0.129). Factors affecting the performance of the classification at this particular study site include the smooth transition in vegetation structure between intermediate and late successional stages, and the low sensitivity of NDVI to variations in vertical structure at high biomass levels. ResumoNesse estudo, testamos se uma técnica de classificação existente, baseada na integração de imagens LANDSAT ETM+ e os dados de inventário florestal, permite a caracterização detalhada dos estádios sucessionais em uma área de floresta tropical úmida. Os objetivos específicos foram: (1) mapear classes de idade florestal na Estação Biológica La Selva, na Costa Rica, e (2) quantificar as incertezas da abordagem proposta em relação aos dados de campo e mapas de vegetação existente. Apesar de terem sido detectadas relações significativas entre dados ETM+ e medidas de entropia da altura da vegetação (um substituto para a idade florestal) o sistema de classificação testados nesse estudo não se demonstrou adequado para caracterizar a variação espacial em idade em La Selva, como evidenciado pela matriz de erro e o baixo coeficiente Kappa (0,129). Fatores que afetam o desempenho da classificação área de estudo em particular, incluem a alta similaridade estrutural entre os estádios sucessionais intermediário e avançado, e a baixa sensibilidade do NDVI a variações na estrutura vertical da biomassa em áreas com níveis elevados de biomassa

Hygienisation, gentrification, and urban displacement in Brazil

This article engages recent debates over gentrification and urban displacement in the global South. While researchers increasingly suggest that gentrification is becoming widespread in “Southern” cities, others argue that such analyses overlook important differences in empirical context and privilege EuroAmerican theoretical frameworks. To respond to this debate, in this article, we outline the concept of higienização (hygienisation), arguing that it captures important contextual factors missed by gentrification. Hygienisation is a Brazilian term that describes a particular form of urban displacement, and is directly informed by legacies of colonialism, racial and class stigma, informality, and state violence. Our objective is to show how “Southern” concepts like hygienisation help urban researchers gain better insight into processes of urban displacement, while also responding to recent calls to decentre and provincialise urban theory

Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column

Population Pharmacokinetic Study of a Test Dose Busulfan Patients Undergoing Hematopoietic Stem Cell Transplantation

UNIFESP (Universidade Federal de São Paulo), BrazilOnco-Hematology Unit, Instituto da Criança - HC - FMUSP, Sao Paulo, BrazilHospital Israelita Albert Einstein, BrazilHematology and Bone Marrow Transplantation Dept, Hospital Israelita Albert Einstein, BrazilHematology and Bone Marrow Transplantation Dept, UNIFESP (Universidade Federal de Sao Paulo), BrazilPediatric Bone Marrow Transplantation Center, Instituto de Oncologia Pediatrica, São Paulo, BrazilHematology and Bone Marrow Transplantation Dept, Hospital Israelita Albert Einstein, Sao Paulo, BrazilInstituto de Oncologia Pediátrica, São Paulo, BrazilClinical Research Center, Instituto de Oncologia Pediátrica, São Paulo, BrazilDepartment of Medicine - Bone Marrow Transplant Program, Case Western Reserve University, ClevelandUNIFESP (Universidade Federal de São Paulo), BrazilHematology and Bone Marrow Transplantation Dept, UNIFESP (Universidade Federal de Sao Paulo), BrazilWeb of Scienc

Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines

Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment.e FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/ 2013—FP XII-BIOPLAT), Barretos Cancer Hospital, CAPES, CNPq, FAPEMIG, UFSJ. RMR is a recipient of CNPq Productivity Gran

Estimating aboveground biomass in tropical forests: Field methods and error analysis for the calibration of remote sensing observations

Mapping and monitoring of forest carbon stocks across large areas in the tropics will necessarily rely on remote sensing approaches, which in turn depend on field estimates of biomass for calibration and validation purposes. Here, we used field plot data collected in a tropical moist forest in the central Amazon to gain a better understanding of the uncertainty associated with plot-level biomass estimates obtained specifically for the calibration of remote sensing measurements. In addition to accounting for sources of error that would be normally expected in conventional biomass estimates (e.g., measurement and allometric errors), we examined two sources of uncertainty that are specific to the calibration process and should be taken into account in most remote sensing studies: the error resulting from spatial disagreement between field and remote sensing measurements (i.e., co-location error), and the error introduced when accounting for temporal differences in data acquisition. We found that the overall uncertainty in the field biomass was typically 25% for both secondary and primary forests, but ranged from 16 to 53%. Co-location and temporal errors accounted for a large fraction of the total variance (<65%) and were identified as important targets for reducing uncertainty in studies relating tropical forest biomass to remotely sensed data. Although measurement and allometric errors were relatively unimportant when considered alone, combined they accounted for roughly 30% of the total variance on average and should not be ignored. Our results suggest that a thorough understanding of the sources of error associated with field-measured plot-level biomass estimates in tropical forests is critical to determine confidence in remote sensing estimates of carbon stocks and fluxes, and to develop strategies for reducing the overall uncertainty of remote sensing approaches. © 2017 by the authors; licensee MDPI, Basel, Switzerland

Foundation Pattern, Productivity and Colony Success of the Paper Wasp, Polistes versicolor

Polistes versicolor (Olivier) (Hymenoptera: Vespidae) colonies are easily found in anthropic environments; however there is little information available on biological, ecological and behavioral interactions of this species under these environmental conditions. The objective of this work was to characterize the foundation pattern, the productivity, and the success of colonies of P. versicolor in anthropic environments. From August 2003 to December 2004, several colonies were studied in the municipal district of Juiz de Fora, Southeastern Brazil. It was possible to determine that before the beginning of nest construction the foundress accomplishes recognition flights in the selected area, and later begins the construction of the peduncle and the first cell. As soon as new cells are built, the hexagonal outlines appear and the peduncle is reinforced. Foundation of nests on gypsum plaster was significantly larger (p < 0.0001; χ2 test) in relation to the other types of substrate, revealing the synantropism of the species. On average, the P. versicolor nest presents 244.2 ± 89.5 (100–493) cells and a medium production of 171.67 ± 109.94 (37–660) adults. Cells that produced six individuals were verified. Usually, new colonies were founded by an association of females, responsible for the success of 51.5%. Although these results enlarge knowledge on the foundation pattern of P. versicolor in anthropic environments, other aspects of the foundation process require further investigation

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets