Rules, Standards, and the Internal Point of View

Large scale structure and cosmolog

Pre-clinical validation of a vaginal cream containing copaiba oil (reproductive toxicology study)

AbstractThe aims of this study was to evaluate the effects of oil-resin of Copaiba (Copaifera duckei Dwyer), aired in vaginal cream on the reproductive performance of female rats (Rattus norvegicus). To determine the components of the C. duckei oleoresin, gas chromatography coupled with mass spectrometry (CG–MS) was used, and considering the trans-caryophyllene sesquiterpene as a phytochemical marker in the oleoresin. Due to the extensive use of copaiba oleoresin in the suppository form for gynecological infections, an evaluation was carried out on the effects of copaiba oleoresin (Copaifera duckei Dwyer), delivered in a vaginal cream, on the reproductive performance of female Wistar rats. For this purpose, three groups (n=5–6/group) of female rats were treated as follows: 1 – vaginal cream of copaiba oleoresin (28.6mg/kg), 2 – base vaginal cream and 3 – control (physiological saline 0.9%), administered intravaginally, for 30 days before pregnancy, and from day zero to day 20 during pregnancy. Laparotomy was performed on the 21st day of pregnancy, followed by the determination of reproductive variables: number of live and dead fetuses, mass of the fetuses and placentas, number of implantations and resorptions, number of corpora lutea, pre- and post-implantation loss, and analyses of the fetuses with regard to external and internal anomalies and/or malformations (skeletal and visceral). The trans-caryophyllene present in the sample is suggested as a phytochemical marker and the results of this study demonstrate an absence of maternal toxicity and foetotoxicity embryofoetotoxicity at the dose administered, corresponding to ten times the recommended dose for use in humans. Accordingly, no significant statistical difference was observed between the treated and control groups, for the variables analyzed.Thus, it is concluded that the vaginal cream containing 2.5% copaiba oleoresin is safe during gestation, in female rats (Rattus norvegicus) of the Wistar strain

Sustainable Energy Management of Institutional Buildings through Load Prediction Models: Review and Case Study

Institutional buildings need smart techniques to predict the energy consumption in a smart grids’ framework. Here, the importance of dynamic load forecasting as a tool to support the decision in smart grids is addressed. In addition, it is reviewed the energy consumption patterns of institutional buildings and the state-of-the-art of load forecast modeling using artificial neural networks. The discussion is supported by historical data from energy consumption in a university building. These data are used to develop a reliable model for the prediction of the electric load in a campus. A neural network model was developed, which can forecast the load with an average error of 6.5%, and this model can also be used as a decision tool to assess the convenience of supplying this load with a set of renewable energy sources. Statistical data that measure the availability of the local renewable sources can be compared with a load model in order to assess how well these energy sources match the energy needs of buildings. This novel application of load models was applied to the campus where a good correlation (Pearson coefficient of 0.803) was found between energy demand and the availability of the solar resource in the campus

Comportamento Per Se De Híbridos De Capim-elefante Para Fins Energéticos

The aim of this study was to evaluate the Per se performance of elephant grass hybrids obtained by partial diallel crossing and also their parents for energy purposes through agronomic traits during rainy and dry seasons. The experiment was conducted at Pesagro experimental station, Campos dos Goytacazes, RJ, Brazil, in randomized blocks, with three repetitions. The crosses were made between contrasting parents in a partial diallel scheme with 5 female parents × 5 male parents. The treatments consisted of ten parents, twenty-five hybrid combinations and the control. Three cuts were realized for evaluations (April and October 2013 and April 2014). The characteristics evaluated were: plant height (ALT), in m; stem diameter (SD), in cm; leaf blade width (LB), in cm; number of tillers per linear meter (NP); dry matter percentage (%DM) and dry matter production (DMP), in t.ha-1. Variance analysis were performed and the means were grouped according to Scott-Knott test (P>0.05). It was oberved that the hybrid H4 (Cuban Pinda x Taiwan A-144), H7 (Cameroon - Piracicaba × Três Rios), H8 (Cameroon - Piracicaba × Mercker 86-Mexico), H10 (Cameroon - Piracicaba × Roxo), H13 (P241-86-Piracicaba × Mercker 86-Mexico), H17 (IAC Campinas × Três Rios) and H18 (IAC-86-Campinas × Mercker 86-Mexico) presented high biomass production.71738

Optical mapping compendium of structural variants across global cattle breeds

Structural variants (SV) have been linked to important bovine disease phenotypes, but due to the difficulty of their accurate detection with standard sequencing approaches, their role in shaping important traits across cattle breeds is largely unexplored. Optical mapping is an alternative approach for mapping SVs that has been shown to have higher sensitivity than DNA sequencing approaches. The aim of this project was to use optical mapping to develop a high-quality database of structural variation across cattle breeds from different geographical regions, to enable further study of SVs in cattle. To do this we generated 100X Bionano optical mapping data for 18 cattle of nine different ancestries, three continents and both cattle sub-species. In total we identified 13,457 SVs, of which 1,200 putatively overlap coding regions. This resource provides a high-quality set of optical mapping-based SV calls that can be used across studies, from validating DNA sequencing-based SV calls to prioritising candidate functional variants in genetic association studies and expanding our understanding of the role of SVs in cattle evolution

A Model for the Development of the Rhizobial and Arbuscular Mycorrhizal Symbioses in Legumes and Its Use to Understand the Roles of Ethylene in the Establishment of these two Symbioses

We propose a model depicting the development of nodulation and arbuscular mycorrhizae. Both processes are dissected into many steps, using Pisum sativum L. nodulation mutants as a guideline. For nodulation, we distinguish two main developmental programs, one epidermal and one cortical. Whereas Nod factors alone affect the cortical program, bacteria are required to trigger the epidermal events. We propose that the two programs of the rhizobial symbiosis evolved separately and that, over time, they came to function together. The distinction between these two programs does not exist for arbuscular mycorrhizae development despite events occurring in both root tissues. Mutations that affect both symbioses are restricted to the epidermal program. We propose here sites of action and potential roles for ethylene during the formation of the two symbioses with a specific hypothesis for nodule organogenesis. Assuming the epidermis does not make ethylene, the microsymbionts probably first encounter a regulatory level of ethylene at the epidermis–outermost cortical cell layer interface. Depending on the hormone concentrations there, infection will either progress or be blocked. In the former case, ethylene affects the cortex cytoskeleton, allowing reorganization that facilitates infection; in the latter case, ethylene acts on several enzymes that interfere with infection thread growth, causing it to abort. Throughout this review, the difficulty of generalizing the roles of ethylene is emphasized and numerous examples are given to demonstrate the diversity that exists in plants