Manufacturing Logistics and Packaging Management Using RFID

none2The chapter is centred on the analysis of internal flow traceability of goods (products and/or packaging) along the supply chain by an Indoor Positioning System (IPS) based on Radio Frequency IDentification (RFID) technology. A typical supply chain is an end-to-end process with the main purpose of production, transportation, and distribution of products. It is relative to the products’ movements from the supplier to the manufacturer, distributor, retailer and finally to the end consumer. Moreover, a supply chain is a complex amalgam of parties that require coordination, collaboration, and information exchange among them to increase productivity and efficiency [1, 2]. A supply chain is made up of people, activities, and resources involved in moving products from suppliers to customers and information from customers to suppliers. For this reason, the traceability of logistics flows (physical and information) is a very important issue for the definition and design of manufacturing processes, improvement of layout and increase of security in work areas. European Parliament (Regulation (EC) No. 178/2002) [3] makes it compulsory to trace goods and record all steps, used materials, manufacturing processes, etc. during the entire life cycle of a product [4]. According to the European Parliament, companies recognize the need and importance of tracing materials in indoor environments. Traditionally, the traceability system is performed through the asynchronous fulfilment of checkpoints (i.e. doorways) by materials. In such cases, the tracking is manual, executed by operators. Often companies are not aware of the inefficiencies due to these systems of traceability such as low precision and accuracy in measurements (i.e. no information between doorways), more time spent by operators and costs (due to the full-effort of operators who have to trace target positions and movements). According to [5] every day millions of transport units (cases, boxes, pallets, and containers) are managed worldwide with limited or even with lack of knowledge regarding their status in real-time. In order to overcome the lack of data due to traceability, automatic identification procedures (Auto-ID) could be a solution. They have become very popular in many service industries, purchasing and distribution logistics, manufacturing companies and material flow systems. Automatic identification procedures provide information about people, vehicles, goods, and products in transit within the company [6]. It is possible to note several advantages using an automatic identification system such as the reduction of theft, increase of security during the transport and distribution of assets, and increase of knowledge of objects’ position in real-time. Automatic identification procedures can also be applied to packaging products, instead of to each item contained in the package. Packaging is becoming the cornerstone of processing activities [7]. Sometimes products are very expensive and packages contain important and critical goods (for example dangerous or explosive materials) and the tracking of goods – and packaging in particular – is a critical function. The main advantage of automatic system application to packages is the possibility to map the path of all items contained into the packages and to find out their real-time position. The installation of automatic systems in packages allows costs and time to be reduced (by installing, for example, the tag directly on the package instead of on each product contained inside the package). The purpose of the chapter is to provide an innovative automatic solution for the traceability of everything that moves within a company, in order to simplify and improve the process of logistics flow traceability and logistics optimization. The chapter deals with experimental research that consists of several tests, static and dynamic, tracing the position (static) and movements (dynamic) of targets (e.g. people, vehicles, objects) in indoor environments. In order to identify the best system to use in the real-time traceability of products, the authors have chosen Real Time Location Systems (RTLSs) and, in particular, the Indoor Positioning Systems (IPSs) based on Radio Frequency IDentification (RFID) technology. The authors discuss the RFID based system using UWB technology, both in terms of design of the system and real applications. The chapter is organized as follows: Section 2 briefly describes IPS systems, looking in more depth at RFID technology. After that the experimental research with the relative results and discussion are described in Section 3. Section 4 presents an analysis of RFID traceability systems applied to packaging. Conclusions and further research are discussed in Section 5.mixedREGATTIERI A.; SANTARELLI GREGATTIERI A.; SANTARELLI

The Important Role of Packaging in Operations Management

The chapter focuses on the analysis of the impact of packaging in Operations Management (OM) along the whole supply chain. The product packaging system (i.e. primary, secondary and tertiary packages and accessories) is highly relevant in the supply chain and its importance is growing because of the necessity to minimize costs, reduce the environmental impact and also due to the development of web operations (i.e. electronic commerce). A typical supply chain is an end-to-end process with the main purpose of production, transportation, and distribution of products. It is relative to the products\u2019 movements normally from the supplier to the manufacturer, distributor, retailer and finally the end consumer. All products moved are contained in packages and for this reason the analysis of the physical logistics flows and the role of packaging is a very important issue for the definition and design of manufacturing processes, improvement of layout and increase in companies\u2019 efficiency. In recent years, companies have started to consider packaging as a critical issue. It is necessary to analyse the packages\u2019 characteristics (e.g. shape, materials, transport, etc.) in order to improve the performance of companies and minimize their costs. Packaging concerns all activities of a company: from the purchasing of raw materials to the production and sale of finished products, and during transport and distribution. In order to manage the activities directly linked with the manufacturing of products (and consequently with the packaging system), the OM discipline is defined. It is responsible for collecting various inputs and converting them into desired outputs through operations [1]. Recently, more and more companies have started to use web operations. Electronic commerce (e-commerce) is the most promising application of information technology witnessedin recent years. It is revolutionising supply chain management and has enormous potential for manufacturing, retail and service operations. The role of packaging changes with the increase in the use of e-commerce: from the traditional \u201cshop window\u201d it has become a means of information and containment of products. The purpose of the chapter is to briefly describe a model of OM discipline usable to highlight the role of packaging along the supply chain, describing different implications of an efficient product packaging system for successful management of operations. Particular attention is paid to the role of product packaging in modern web operations. The chapter is organised as follows: Section 2 presents a brief description of OM in order to engage the topic of packaging. The packaging logistics system is described in Section 3, before presenting experimental results of studies dealing with packaging perception by both companies and customers [2; 3]. Moreover, Section 3 introduces the packaging logistics system also including the analysis of the role of packaging in OM and a description of a complete mathematical model for the evaluation of total packaging cost is presented. Section 4 presents background about modern e-commerce and its relationship with OM. Packaging and e-commerce connected with OM is described in Section 5 and a case study on packaging e-commerce in operations is analysed in Section 6. Finally, the conclusion and further research are presented

The fundamental role of the packaging system along the supply chain

Nowadays, packaging assumes a fundamental role along the entire supply chain and will continue to do so to an even greater extent in years to come. After establishing legitimacy in the 1950s, packaging today has entered a mature phase and it is at the centre of complex distributional and communicational issues. Packaging systems have also become considerably more important for the minimisation of environmental impact. The present thesis focuses on the aforementioned issues, elaborating on current strategies and methods, as well as presenting new innovative approaches for effective design, management and optimisation of packaging systems. The work begins with a general description of packaging systems, with an analysis of their origin, functions and roles throughout the supply chain. Based on this study, a reference framework for packaging is then developed, defined by five key drivers: marketing, design, logistics, cost and environmental impact. Each driver is examined and studied using different methodologies. An empirical study allows definition of a reference benchmark on packaging, while a case study demonstrates the implementation of the developed approaches in a real industrial setting. Real packaging prototypes are developed, as well as various products that must meet specific needs, starting from cardboard, plastic and wooden packages. Relevant results from both economic and environmental standpoints are presented with regard to the issues surrounding product packaging. Potential for cost reductions and increases in management efficiency of packaging companies are identified. It is shown that the environmental impact of packaging systems may be reduced through their re-use for both similar and very different functions to those for which packaging is traditionally employed: product protection and containment

Expression and glucocorticoid-dependent regulation of the stressinducible protein DRR1 in the mouse adult brain

Identifying molecular targets that are able to buffer the consequences of stress and therefore restore brain homeostasis is essential to develop treatments for stress-related disorders. Down-regulated in renal cell carcinoma 1 (DRR1) is a unique stress-induced protein in the brain and has been recently proposed to modulate stress resilience. Interestingly, DRR1 shows a prominent expression in the limbic system of the adult mouse. Here, we analyzed the neuroanatomical and cellular expression patterns of DRR1 in the adult mouse brain using in situ hybridization, immunofluorescence and Western blot. Abundant expression of DRR1 mRNA and protein was confirmed in the adult mouse brain with pronounced differences between distinct brain regions. The strongest DRR1 signal was detected in the neocortex, the CA3 region of the hippocampus, the lateral septum and the cerebellum. DRR1 was also present in circumventricular organs and its connecting regions. Additionally, DRR1 was present in non-neuronal tissues like the choroid plexus and ependyma. Within cells, DRR1 protein was distributed in a punctate pattern in several subcellular compartments including cytosol, nucleus as well as some pre- and postsynaptic specializations. Glucocorticoid receptor activation (dexamethasone 10 mg/kg s.c.) induced DRR1 expression throughout the brain, with particularly strong induction in white matter and fiber tracts and in membrane-rich structures. This specific expression pattern and stress modulation of DRR1 point to a role of DRR1 in regulating how cells sense and integrate signals from the environment and thus in restoring brain homeostasis after stressful challenges

Evaluation of Novel Guanidino-Containing Isonipecotamide Inhibitors of Blood Coagulation Factors against SARS-CoV-2 Virus Infection

Coagulation factor Xa (fXa) and thrombin (thr) are widely expressed in pulmonary tissues, where they may catalyze, together with the transmembrane serine protease 2 (TMPRSS2), the coronaviruses spike protein (SP) cleavage and activation, thus enhancing the SP binding to ACE2 and cell infection. In this study, we evaluate in vitro the ability of approved (i.e., dabigatran and rivaroxaban) and newly synthesized isonipecotamide-based reversible inhibitors of fXa/thr (cmpds 1-3) to hinder the SARS-CoV-2 infectivity of VERO cells. Nafamostat, which is a guanidine/amidine antithrombin and antiplasmin agent, disclosed as a covalent inhibitor of TMPRSS2, was also evaluated. While dabigatran and rivaroxaban at 100 mu M concentration did not show any effect on SARS-CoV-2 infection, the virus preincubation with new guanidino-containing fXa-selective inhibitors 1 and 3 did decrease viral infectivity of VERO cells at subtoxic doses. When the cells were pre-incubated with 3, a reversible nanomolar inhibitor of fXa (K-i = 15 nM) showing the best in silico docking score toward TMPRSS2 (pdb 7MEQ), the SARS-CoV-2 infectivity was completely inhibited at 100 mu M (p < 0.0001), where the cytopathic effect was just about 10%. The inhibitory effects of 3 on SARS-CoV-2 infection was evident (ca. 30%) at lower concentrations (3-50 mu M). The covalent TMPRSS2 and the selective inhibitor nafamostat mesylate, although showing some effect (15-20% inhibition), did not achieve statistically significant activity against SARS-CoV-2 infection in the whole range of test concentrations (3-100 mu M). These findings suggest that direct inhibitors of the main serine proteases of the blood coagulation cascade may have potential in SARS-CoV-2 drug discovery. Furthermore, they prove that basic amidino-containing fXa inhibitors with a higher docking score towards TMPRSS2 may be considered hits for optimizing novel small molecules protecting guest cells from SARS-CoV-2 infection

Identification and Characterization of Cancer Stem Cells from Head and Neck Squamous Cell Carcinoma Cell Lines

Background/Aims: Head and neck squamous cell carcinoma (HNSCC) ranks sixth worldwide for tumor-related mortality. A subpopulation of tumor cells, termed cancer stem cells (CSCs), has the ability to support cancer growth. Therefore, profiling CSC-enriched populations could be a reliable tool to study cancer biology. Methods: We performed phenotypic characterization of 7 HNSCC cell lines and evaluated the presence of CSCs. CSCs from Hep-2 cell line and HNSCC primary cultures were enriched through sphere formation and sphere-forming cells have been characterized both in vitro and in vivo. In addition, we investigated the expression levels of Nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in several malignancies. Results: CSC markers were markedly expressed in Hep-2 cell line, which was found to be highly tumorigenic. CSC-enriched populations displayed increased expression of CSC markers and a strong capability to form tumors in vivo. We also found an overexpression of CSC markers in tumor formed by CSC-enriched populations. Interestingly, NNMT levels were significantly higher in CSC-enriched populations compared with parental cells. Conclusion: Our study provides an useful procedure for CSC identification and enrichment in HNSCC. Moreover, results obtained seem to suggest that CSCs may represent a promising target for an anticancer therapy

Self-employment and entrepreneurship in urban and rural labour markets

We study the link between self-employment and some salient aspects of entrepreneurship - namely business creation and innovation - in urban and rural labour markets. In order to do so, we combine individual and firm-level data for Britain aggregated at the Travel-to-Work Area level. We find that a higher incidence of self-employment positively and strongly correlates with business creation and innovation in urban areas, but not in rural areas. We also document that more rural than urban workers become self-employed in areas with comparably poor labour market opportunities, although this heterogeneity is not evident when focussing on entrepreneurship. Finally, we show that the misalignment between self-employment and our proxies for entrepreneurship in rural areas disappears once we account for local labour market conditions. Our results suggest that self-employment, business creation and innovation are well lined-up in urban areas because they capture the same economic phenomenon - namely, genuine entrepreneurship. This is not the case for rural areas

BAAV Mediated GJB2 Gene Transfer Restores Gap Junction Coupling in Cochlear Organotypic Cultures from Deaf Cx26Sox10Cre Mice

The deafness locus DFNB1 contains GJB2, the gene encoding connexin26 and GJB6, encoding connexin30, which appear to be coordinately regulated in the inner ear. In this work, we investigated the expression and function of connexin26 and connexin30 from postnatal day 5 to adult age in double transgenic Cx26Sox10Cre mice, which we obtained by crossing connexin26 floxed mice with a deleter Sox10–Cre line. Cx26Sox10Cre mice presented with complete connexin26 ablation in the epithelial gap junction network of the cochlea, whereas connexin30 expression was developmentally delayed; immunolabeling patterns for both connexins were normal in the cochlear lateral wall. In vivo electrophysiological measurements in Cx26Sox10Cre mice revealed profound hearing loss accompanied by reduction of endocochlear potential, and functional experiments performed in postnatal cochlear organotypic cultures showed impaired gap junction coupling. Transduction of these cultures with a bovine adeno associated virus vector restored connexin26 protein expression and rescued gap junction coupling. These results suggest that restoration of normal connexin levels by gene delivery via recombinant adeno associated virus could be a way to rescue hearing function in DFNB1 mouse models and, in future, lead to the development of therapeutic interventions in humans

Unraveling the potential of graphene quantum dots against Mycobacterium tuberculosis infection

IntroductionThe emergence of drug-resistant Mycobacterium tuberculosis (Mtb) strains has underscored the urgent need for novel therapeutic approaches. Carbon-based nanomaterials, such as graphene oxide (GO), have shown potential in anti-TB activities but suffer from significant toxicity issues.MethodsThis study explores the anti-TB potential of differently functionalized graphene quantum dots (GQDs) – non-functionalized, L-GQDs, aminated (NH2-GQDs), and carboxylated (COOH-GQDs) – alone and in combination with standard TB drugs (isoniazid, amikacin, and linezolid). Their effects were assessed in both axenic cultures and in vitro infection models.ResultsGQDs alone did not demonstrate direct mycobactericidal effects nor trapping activity. However, the combination of NH2-GQDs with amikacin significantly reduced CFUs in in vitro models. NH2-GQDs and COOH-GQDs also enhanced the antimicrobial activity of amikacin in infected macrophages, although L-GQDs and COOH-GQDs alone showed no significant activity.DiscussionThe results suggest that specific types of GQDs, particularly NH2-GQDs, can enhance the efficacy of existing anti-TB drugs. These nanoparticles might serve as effective adjuvants in anti-TB therapy by boosting drug performance and reducing bacterial counts in host cells, highlighting their potential as part of advanced drug delivery systems in tuberculosis treatment. Further investigations are needed to better understand their mechanisms and optimize their use in clinical settings