Antifungal activity of mangrove rhizobacterium Pseudomonas aeruginosa against certain phytopathogenic fungi and its growth characterization

Antimicrobial substances are widespread and they are likely to play an important protective role. Marine bacterium has been recognized as producer of important antimicrobial substances which has an exceedingly bright future in the discovery of life saving drugs. The present study was carried out to screen the antifungal activity of mangrove rhizobacteria against certain phyto pathogens from Manakudi estuary, Kanyakumari District, Tamilnadu. Around 20 colonies obtained in Zobell marine agar plates were screened for antifungal traits. Among the 20 isolates, the candidate bacterial isolate exhibited good anti fungal ability. Identification of strains was carried out and confirmed by cultural, biochemical and 16S rDNA sequences. The potent strain was identified as Pseudomonas aeruginosa. Various process factors such as different pH, temperature, carbon and nitrogen sources and NaCl were tested for the bacterial growth in static and shaking conditions. The isolated Pseudomonas aeruginosa possesed a variety of promising properties that favoured as a better biocontrol agent. In the present investigation antifungal activity of the mangrove isolate was tested against common pathogens like Penicillium sp., Candida sp., Aspergillus sp., Aspergillus fumigatus, Aspergillus flavus, Pescalotionbsis sp., Fusarium oxysporum and Glomerella cinculata. The candidate bacterium showed inhibitory action to the tested fungal pathogens except Fusarium oxysporum and Glomerella cinculata.

EXPERIMENTAL STUDIES ON PREMIXED CHARGE COMPRESSION IGNITION (PCCI) ENGINE USING PORT INJECTION OF HEATED DIESEL

Port fuel injection is a proven technique in gasoline engine for its simplicity and cost effectiveness. This technique is a remarkable successor to conventional carburetors in reducing emission and improving transient performance. Nowadays, port injection technique is widely researched in Homogenous Charge Compression Ignition (HCCI), Premixed Charge Compression Ignition (PCCI) and Reactivity Controlled Compression Ignition (RCCI) systems, which reduce NOx and soot emission significantly. However, usage of high viscous fuels like diesel in the above techniques has to overcome various challenges like improper vaporization, wall wetting and poor atomization. This article aims to identify the way to obtain a typical diesel spray similar to that of gasoline spray in port injection by elevating the fuel temperature and injection pressure. The results show that the diesel spray at an elevated temperature of 80°C and an injection pressure of 4 bar looked similar to that of gasoline spray (at 33°C and 3 bar). Further experimental studies on engine was performed in Diesel - PCCI mode to study the influence of heated diesel over unheated diesel spray on engine performance and emissions

Marine fisheries of the south-east coast of India during 2008

The south-east coast of India comprising the states of Andhra Pradesh, Tamil Nadu and Pondicherry have a total coastline of 2050 km which is 34% of the total coastline of the country. This region is more diverse with respect to the number of species that are landed. In 2007, it was observed that 499 species were landed in Tamil Nadu, 294 in Andhra Pradesh and 115 in Pondicherry

Implementing Fine/Medium Grained TLP Support in a Many-Core Architecture

Abstract. We believe that future many-core architectures should support a simple and scalable way to execute many threads that are generated by parallel programs. A good candidate to implement an efficient and scalable execution of threads is the DTA (Decoupled Threaded Architecture), which is designed to exploit fine/medium grained Thread Level Parallelism (TLP) by using a hardware scheduling unit and relying on existing simple cores. In this paper, we present an initial implementation of DTA concept in a many-core architecture where it interacts with other architectural components designed from scratch in order to address the problem of scalability. We present initial results that show the scalability of the solution that were obtained using a many-core simulator written in SARCSim (a variant of UNISIM) with DTA support

Coarse-grained reconfigurable array architectures

Coarse-Grained Reconfigurable Array (CGRA) architectures accelerate the same inner loops that benefit from the high ILP support in VLIW architectures. By executing non-loop code on other cores, however, CGRAs can focus on such loops to execute them more efficiently. This chapter discusses the basic principles of CGRAs, and the wide range of design options available to a CGRA designer, covering a large number of existing CGRA designs. The impact of different options on flexibility, performance, and power-efficiency is discussed, as well as the need for compiler support. The ADRES CGRA design template is studied in more detail as a use case to illustrate the need for design space exploration, for compiler support and for the manual fine-tuning of source code

Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries

Preeclampsia is accompanied by an extensive remodeling of the extracellular matrix of umbilical cord. It is associated with an increase in collagen content in the umbilical cord artery. Furthermore, preeclampsia distinctly reduces proteolytic and gelatinolytic activity, especially after activation with various agents

The role of oxidative stress and antioxidant supplementation in pregnancy disorders

Oxidative stress is widely implicated in failed reproductive performance including infertility, miscarriage, diabetes-related congenital malformations and preeclampsia. Maternal obesity is a strong risk factor for preeclampsia, and recently, in an animal model of maternal obesity we have reported evidence of oxidative stress in the oocytes of obese animals prior to pregnancy as well as in early stage embryos. This adds to the growing evidence for a greater focus on the pre-conceptual period in prevention of pregnancy disorders including those related to oxidative stress. Our research has also focussed on the role of free radicals and antioxidant capacity in preeclampsia. Assessment by measurement of markers of lipid peroxidation or of antioxidant capacity has provided unequivocal evidence for oxidative stress in this disorder. Partial failure of the process of placentation has been implicated, with recent evidence proposing that ischaemia-reperfusion in the placenta may contribute to oxidative stress in trophoblast. Endoplasmic reticulum stress in the placenta may also play a role. We and others have performed randomised controlled trials to determine whether early supplementation with vitamins C and E in women at risk of preeclampsia may be beneficial but these studies have shown no evidence for prevention of preeclampsia. Whether this represents an inappropriate antioxidant strategy or whether supplementation has been too late in gestation to be beneficial is not known. Other potential approaches to prevention of preeclampsia through amelioration of oxidative stress include provision of supplements in the pre-conceptual period, selenium supplements, anti-peroxynitrite strategies and statins

Methionine Sulfoxide Reductase A (MsrA) Deficient Mycoplasma genitalium Shows Decreased Interactions with Host Cells

Mycoplasma genitalium is an important sexually transmitted pathogen that affects both men and women. In genital-mucosal tissues, it initiates colonization of epithelial cells by attaching itself to host cells via several identified bacterial ligands and host cell surface receptors. We have previously shown that a mutant form of M. genitalium lacking methionine sulfoxide reductase A (MsrA), an antioxidant enzyme which converts oxidized methionine (Met(O)) into methionine (Met), shows decreased viability in infected animals. To gain more insights into the mechanisms by which MsrA controls M. genitalium virulence, we compared the wild-type M. genitalium strain (G37) with an msrA mutant (MS5) strain for their ability to interact with target cervical epithelial cell lines (HeLa and C33A) and THP-1 monocytic cells. Infection of epithelial cell lines with both strains revealed that MS5 was less cytotoxic to HeLa and C33A cell lines than the G37 strain. Also, the MS5 strain was more susceptible to phagocytosis by THP-1 cells than wild type strain (G37). Further, MS5 was less able to induce aggregation and differentiation in THP-1 cells than the wild type strain, as determined by carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling of the cells, followed by counting of cells attached to the culture dish using image analysis. Finally, MS5 was observed to induce less proinflammatory cytokine TNF-α by THP-1 cells than wild type G37 strain. These results indicate that MsrA affects the virulence properties of M. genitalium by modulating its interaction with host cells

Iron Behaving Badly: Inappropriate Iron Chelation as a Major Contributor to the Aetiology of Vascular and Other Progressive Inflammatory and Degenerative Diseases

The production of peroxide and superoxide is an inevitable consequence of aerobic metabolism, and while these particular "reactive oxygen species" (ROSs) can exhibit a number of biological effects, they are not of themselves excessively reactive and thus they are not especially damaging at physiological concentrations. However, their reactions with poorly liganded iron species can lead to the catalytic production of the very reactive and dangerous hydroxyl radical, which is exceptionally damaging, and a major cause of chronic inflammation. We review the considerable and wide-ranging evidence for the involvement of this combination of (su)peroxide and poorly liganded iron in a large number of physiological and indeed pathological processes and inflammatory disorders, especially those involving the progressive degradation of cellular and organismal performance. These diseases share a great many similarities and thus might be considered to have a common cause (i.e. iron-catalysed free radical and especially hydroxyl radical generation). The studies reviewed include those focused on a series of cardiovascular, metabolic and neurological diseases, where iron can be found at the sites of plaques and lesions, as well as studies showing the significance of iron to aging and longevity. The effective chelation of iron by natural or synthetic ligands is thus of major physiological (and potentially therapeutic) importance. As systems properties, we need to recognise that physiological observables have multiple molecular causes, and studying them in isolation leads to inconsistent patterns of apparent causality when it is the simultaneous combination of multiple factors that is responsible. This explains, for instance, the decidedly mixed effects of antioxidants that have been observed, etc...Comment: 159 pages, including 9 Figs and 2184 reference

Highly Cited Works In Covid-19 - The Global Perspective

Research papers published covid-19 during 1989-2020 and cited at least 100 times. It is founded that 72 countries contributed a total 1000 highly cited research publications and received 227766 Citations (Average Citation Per Articles is 227.766 and Cited References 51830). United States of America (USA) topped in the table with highest citations (86603) for 432 publications followed by China with 83829 citations for 280 Publications, United Kingdom (UK) with 29372 citations for 122 publications. It is also important to note that the top 8 countries have more than 10,000 citations that include Netherlands, Germany, Canada, France, and Saudi Arabia. India stands in the list with 500 citations. University Hong Kong has the most citations (28278) followed by Chinese Academy of Sciences with 14513 GCS, Huazhong University of Science & Technology with 12294 Citations and Wuhan University with 10392 citations. LANCET’ is the journal which has got the maximum citations of 22221 (41 records) out of the total 249 Journals followed by ‘Journal of Virology’ with 22039 citations (139 records) ‘NEW ENGLAND JOURNAL OF MEDICINE’ with 21213 citations, ‘PNAS’ with 11216. The prominent journals ‘Nature’ and ‘Science’ share the table with 9639 & 9613 citations respectively. It will surprise few recently published (Feb 2020, April 2020, March 2020) that methods papers lead the list of the most cited scientific papers. “The Huang CL, Wang YM, Li XW, Ren LL, Zhao JP, et al.,” leads with 3915 Citations (Huang CL, Wang YM, Li XW, Ren LL, Zhao JP, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. LANCET. 2020 FEB 15; 395 (10223): 497-506). Notably, Institutes from China dominating in top 10 list, University Hong Kong has the most citations (28278) followed by Chinese Academy of Sciences with 14513 Citations