Caracterización de mieles de obtención artesanal y comerciales producidas en Galicia (NO de España) a partir de su espectro polínico y contenido proteico

An analysis was made of 49 honeys produced in NW of Spain (Ourense-Galicia), 37 of homemade production and 12 commercialized with quality label “Mel de Galicia” (Galician Honey). Its flower origin was determinate by the method of percentage pollen analysis and its protein content by Bradford method. They resulted to be 9 chestnut tree (Castanea sativa) and 6 bramble (Rubus ulmifolius-t.) unifloral, one Cytisus scoparius- t. unifloral and the other honeys was multifloral with predominance of chestnut (Castanea sativa), bramble (Rubus ulmifolius-t.), shrubland (Erica sp.) with gorse (Cytisus scoparius–t.) or heather. It was found 9 homemade honeys belonged to the Maurizio’s Class I, 9 to the Class II, 9 to the Class III, 7 to the Class IV and 3 belong to the Class V. Among the commercial honeys, 4 belong to the Class IV and 8 to the Class V. The value of protein in the honeys varied from 0.8 to 5.2 mg prot./g honey. The average was 1.95mg prot./g honey for homemade honeys, and 3.1 mg prot./g honey for commercial ones. This wide variation resulted as homemade like commercial honeys, and in this last it seem even to be higher.Se han estudiado 49 mieles producidas en el NO de España (Ourense-Galicia), 37 de producción artesanal y 12 comercializadas con etiqueta de calidad “Mel de Galicia”. Se determinó su origen floral, mediante el método de análisis polínico porcentual, y su contenido proteico, mediante el método de Bradford. En cuanto a su origen floral, han resultado ser 9 monoflorales de Castanea sativa, 6 de Rubus ulmifolius-t., una monofloral de Cytisus scoparius-t. y las demás milflores con predominancia de castaño (Castanea sativa), zarza (Rubus ulmifolius-t.), xesta-tojo (Cytisus scoparius-t.) o brezo (Erica sp.). Por su riqueza polínica, se han encontrado, en las mieles artesanales, 9, de clase de Maurizio I; 9, de Clase II; 9, de Clase III; 7, de Clase IV y 3, de Clase V. En las comerciales, 4, de Clase IV y 8, de Clase V. El contenido en proteína de las mieles varió desde 0,8 mg prot./g miel a 5,2 mg prot./g miel. Los valores medios han sido de 1,95, para las de origen artesanal y 3,1, para las comerciales

Reversible Nuclear-Lipid-Droplet Morphology Induced by Oleic Acid: A Link to Cellular-Lipid Metabolism

Neutral lipidsÐinvolved in many cellular processesÐare stored as lipid droplets (LD), thosemainly cytosolic (cLD) along with a small nuclear population (nLD). nLD could be involved innuclear-lipid homeostasis serving as an endonuclear buffering system that would provide orincorporate lipids and proteins involved in signalling pathways as transcription factors andas enzymes of lipid metabolism and nuclear processes. Our aim was to determine if nLDconstituted a dynamic domain. Oleic-acid (OA) added to rat hepatocytes or HepG2 cells inculture produced cellular-phenotypic LD modifications: increases in TAG, CE, C, and PLcontent and in cLD and nLD numbers and sizes. LD increments were reversed on exclusionof OA and were prevented by inhibition of acyl-CoA synthetase (with Triacsin C) and thuslipid biosynthesis. Under all conditions, nLD corresponded to a small population (2±10%) oftotal cellular LD. The anabolism triggered by OA, involving morphologic and size changeswithin the cLD and nLD populations, was reversed by a net balance of catabolism, uponeliminating OA. These catabolic processes included lipolysis and the mobilization of hydrolyzedFA from the LD to cytosolic-oxidation sites. These results would imply that nLD areactively involved in nuclear processes that include lipids. In conclusion, nLD are a dynamicnuclear domain since they are modified by OA through a reversible mechanism in combinationwith cLD; this process involves acyl-CoA-synthetase activity; ongoing TAG, CE, and PLbiosynthesis. Thus, liver nLD and cLD are both dynamic cellular organelles.Fil: Lagrutta, Lucía Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Montero Villegas, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Layerenza, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Sisti, Martin Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Garcia, Margarita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Ves Losada, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentin

Diversity among traditional minority red grape varieties according to their aromatic profile

Free and glycosidically-bound aromatic characterization of 21 minority red grapevine varieties was carried out, along three consecutive vintages, using solid phase microextraction followed by gas chromatography-mass spectrometry methodology (SPME-GC-MS). The two main study aims were to evaluate the possibility of aromatically differentiated varieties based on their origin and to test the aromatic profile for being used as a chemotaxonomic tool. Based on the results obtained in this research, it would be also interesting to verify in future studies if this varietal diversity could translate into a diversification of quality products in the current globalized wine market. A volatile profile was established grouping aroma compounds into thirteen families: acids, alcohols, esters, C6 compounds, thiols, ketones, aldehydes, phenols, terpenes, C13-norisoprenoids, lactones, polycyclic aromatic hydrocarbons (PAHs), and sesquiterpenes. Significant differences were found among varieties for esters, phenols, terpenes, and total compounds in the free fraction and for alcohols, acids, C6 compounds, C13-norisoprenoids, terpenes, sesquiterpenes, and total compounds in the glycosidically-bound fraction. Subtle differentiation between different groups of varieties with common genetic origin was achieved by free aromatic profile (PCA) component analysis. Nevertheless, more in-depth studies are considered necessary to confirm the usefulness of the aromatic profile as a chemotaxonomic tool.Agencia Estatal de Investigación (AEI) – Ministerio de Ciencia e Innovación de España | Ref. BES-2017-082396European Agricultural Fund for Rural Development (EAFRD) | Ref. 12/32/408/280318/1

Financial audit applied to the financial statements of the year 2018 of the company tricomtelecomunicaciones ltda.

El presente trabajo tuvo como objetivo efectuar una auditoría financiera aplicada a los estados financieros del año 2018 de la empresa TRICOMTELECOMUNICACIONES LTDA., en la ciudad de Cúcuta. La metodología de la investigación fue descriptiva, con un enfoque cualitativo y cuantitativo. La investigación permitió identificar las deficiencias actuales de la empresa en cuanto control interno, pues la empresa no posee manual de funciones, procedimientos, ni políticas y controles que le permita direccionar su funcionamiento de forma eficaz. También se hallaron falencias en la apropiación del sistema contable y financiero, precisamente al hallar diferencias en los saldos del sistema contable y lo presentado en el Balance General, especialmente en las cuentas de Caja, Inventarios e Industria y Comercio. En esta medida, se efectuaron una serie de recomendaciones con el fin de mitigar el riesgo inherente y aumentar la confianza y razonabilidad de la información contable y financiera de la organización.The purpose of this work was to carry out a financial audit applied to the financial statements of the year 2018 of the company TRICOMTELECOMUNICACIONES LTDA., in the city of Cúcuta. The research methodology was descriptive, with a qualitative and quantitative approach. The investigation allowed identifying the current deficiencies of the company as an internal control, since the company does not have a manual of functions, procedures, nor policies and controls that allow it to direct its operation in an effective way. In addition, different deficiencies were identified in the appropriation of the accounting and financial system, precisely by finding differences in the balances of the accounting system and what was presented in the Balance Sheet, especially in the Cash, Inventories and Industry and Commerce accounts. In this measure, a series of recommendations were made to management and the accountant in order to mitigate the inherent risk and increase the trustworthiness and reasonableness of the organization's accounting and financial information

Interacciones comunicativas y colaboración mediada por entornos virtuales de aprendizaje universitarios

Teaching and learning scenarios have changed in recent years, surpassing spatial and temporal barriers, mainly due to the COVID-19 pandemic. Virtual Learning Environments (VLEs) have prevailed over face-to-face settings, with technology-mediated education taking on a leading role and giving rise to new forms of knowledge construction and interaction among educational stakeholders. Although new opportunities for interaction exist, the low student participation in VLEs has motivated research aimed to identify alternatives to increase collaboration in e-learning platforms. This article presents the results of a research oriented to how to foster or enhance communicative interactions and collaboration mediated by ICTs in VLEs. The research was conducted using a qualitative perspective with a hermeneutic methodological approach. For this survey, 124 primary studies and 42 VLEs from the Academic UVirtual platform of the University of Medellín, Colombia, were systematized. As part of the results, the following factors were identified: didactic, mediation, technological, and motivation-feedback, which can facilitate communication and collaboration. Such factors also facilitate: (1) the characterization of communicative interactions to promote collaboration; (2) the definition of actions aimed at fostering critical thinking and self-learning skills, and continuous/flexible teacher-student development; and (3) the implementation of enriched VLEs using online tools.Los escenarios de enseñanza-aprendizaje cambiaron en los últimos años superando las barreras espaciotemporales, principalmente desde la pandemia. Los Entornos Virtuales de Aprendizaje (EVA) predominan por encima de los presenciales y logran un papel protagónico en la educación mediada por la tecnología, dando paso a otras formas de construcción del conocimiento y de interacción entre los actores del contexto educativo. Si bien existen nuevas oportunidades de interacción, la baja participación de los estudiantes en los EVA ha motivado investigaciones orientadas a identificar alternativas para incrementar la colaboración en plataformas e-learning. En este artículo se presentan resultados de una investigación alrededor de cómo propiciar o incrementar las interacciones comunicativas y la colaboración mediadas por las TIC en los EVA, desarrollada mediante la perspectiva cualitativa con un enfoque hermenéutico. Se sistematizaron 124 estudios primarios y 42 EVA de la plataforma Uvirtual de la Universidad de Medellín, Colombia. Como parte de los resultados se definieron los factores: didáctico, mediación, tecnológico y motivación-realimentación, a través de los cuales se pueden propiciar la comunicación y la colaboración, y permiten: (1) caracterizar las interacciones comunicativas para fomentar la colaboración; (2) definir acciones orientadas a fomentar habilidades de pensamiento crítico, autoaprendizaje y formación permanente/flexible de profesores-estudiantes; y (3) implementar EVA enriquecidos utilizando herramientas online

Communicative interactions and collaboration mediated by virtual university learning environments

Los escenarios de enseñanza-aprendizaje cambiaron en los últimos años superando las barreras espaciotemporales, principalmente desde la pandemia. Los Entornos Virtuales de Aprendizaje (EVA) predominan por encima de los presenciales y logran un papel protagónico en la educación mediada por la tecnología, dando paso a otras formas de construcción del conocimiento y de interacción entre los actores del contexto educativo. Si bien existen nuevas oportunidades de interacción, la baja participación de los estudiantes en los EVA ha motivado investigaciones orientadas a identificar alternativas para incrementar la colaboración en plataformas e-learning. En este artículo se presentan resultados de una investigación alrededor de cómo propiciar o incrementar las interacciones comunicativas y la colaboración mediadas por las TIC en los EVA, desarrollada mediante la perspectiva cualitativa con un enfoque hermenéutico. Se sistematizaron 124 estudios primarios y 42 EVA de la plataforma Uvirtual de la Universidad de Medellín, Colombia. Como parte de los resultados se definieron los factores: didáctico, mediación, tecnológico y motivación-realimentación, a través de los cuales se pueden propiciar la comunicación y la colaboración, y permiten: (1) caracterizar las interacciones comunicativas para fomentar la colaboración; (2) definir acciones orientadas a fomentar habilidades de pensamiento crítico, autoaprendizaje y formación permanente/flexible de profesores-estudiantes; y (3) implementar EVA enriquecidos utilizando herramientas online.Teaching and learning scenarios have changed in recent years, surpassing spatial and temporal barriers, mainly due to the pandemic. Virtual Learning Environments (VLEs) have prevailed over face-to-face settings, with technology-mediated education taking on a leading role and giving rise to new forms of knowledge construction and interaction among educational stakeholders. Although new opportunities for interaction exist, the low student participation in VLEs has motivated research aimed to identify alternatives to increase collaboration in e-learning platforms. This article presents the results of a research oriented to how to foster or enhance communicative interactions and collaboration mediated by ICTs in VLEs. The research was conducted using a qualitative perspective with a hermeneutic methodological approach. For this survey, 124 primary studies and 42 VLEs from the Academic UVirtual platform of the University of Medellín, Colombia, were systematized. As part of the results, the following factors were identified: didactic, mediation, technological, and motivation-feedback, which can facilitate communication and collaboration. Such factors also facilitate: (1) the characterization of communicative interactions to promote collaboration; (2) the definition of actions aimed at fostering critical thinking and self-learning skills, and continuous/flexible teacher-student development; and (3) the implementation of enriched VLEs using online tools

Configuración de los objetos internos y externos : un determinante en el desarrollo psíquico

Psicólogo (a)Pregrad

Guidelines of an integrated management system under the requirements of NTC-ISO 9001:2015 and NTC-ISO 14001:2015 in institutions of superior education

In order to make a proposal for guidelines to facilitate the integration of systems under NTC-ISO 9001:2015 and NTC-ISO 14001:2015 in higher education institutions (HEI), a sample of six universities certified with Icontec in NTC-ISO 9001, in which an analysis of its master documents was made to identify axes and missionary components that allow the articulation of an integrated management system. Subsequently a diagnosis was made to identify the current state of the environmental management and the integration of these standards. The results show that the quality system is implicit from the strategic planning, is part of its organizational culture and is a tool that contributes to the fulfillment of the mission. With environmental management, this same appropriation is not reflected in the absence of elements such as policies or guidelines that contribute to identify, reduce or control aspects. Finally, with these elements, guidelines were proposed that serve as a tool for the HEI to integrate their quality and environmental management systems and to contribute to the decision-making by top management

Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism

Neutral lipids - involved in many cellular processes - are stored as lipid droplets (LD), those mainly cytosolic (cLD) along with a small nuclear population (nLD). nLD could be involved in nuclear-lipid homeostasis serving as an endonuclear buffering system that would provide or incorporate lipids and proteins involved in signalling pathways as transcription factors and as enzymes of lipid metabolism and nuclear processes. Our aim was to determine if nLD constituted a dynamic domain. Oleic-acid (OA) added to rat hepatocytes or HepG2 cells in culture produced cellular-phenotypic LD modifications: increases in TAG, CE, C, and PL content and in cLD and nLD numbers and sizes. LD increments were reversed on exclusion of OA and were prevented by inhibition of acyl-CoA synthetase (with Triacsin C) and thus lipid biosynthesis. Under all conditions, nLD corresponded to a small population (2-10%) of total cellular LD. The anabolism triggered by OA, involving morphologic and size changes within the cLD and nLD populations, was reversed by a net balance of catabolism, upon eliminating OA. These catabolic processes included lipolysis and the mobilization of hydrolyzed FA from the LD to cytosolic-oxidation sites. These results would imply that nLD are actively involved in nuclear processes that include lipids. In conclusion, nLD are a dynamic nuclear domain since they are modified by OA through a reversible mechanism in combination with cLD; this process involves acyl-CoA-synthetase activity; ongoing TAG, CE, and PL biosynthesis. Thus, liver nLD and cLD are both dynamic cellular organelles.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias MédicasFacultad de Ciencias Exacta

Liver Nuclear Lipid Droplets are a dynamic nuclear domain

The cell nucleus (N) is a highly compartmentalized organelle characterized by several dynamic domains. In the laboratory it was shown for the first time that nuclear lipid droplets (nLD) are a new class of subnuclear bodies and a nuclear domain where neutral lipids are stored and organized. These droplets would be built up around a hydrophobic core of TAG and CE enriched in oleic acid and surrounded by a monolayer of polar lipids along with C and associated proteins. nLD components represent an exceedingly small proportions of the total cell components.Facultad de Ciencias Médica