Online Food Delivery in Tourism Destination: Factors Promoting Intentional Behaviour and eWOM

Online Food Delivery (OFD) has become essential to global people's lives with digital life behaviour preferences. This study seeks to uncover the intrinsic aspects of OFD customers who use this service in the tourist destinations they visit. By involving 307 respondents surveyed online, this study has analyzed the construction of the influence of variables of religiosity, environmental awareness, brand authenticity, ability, motivation, and opportunity, on customer attitudes that lead to intentional behaviour and electronic Word of Mouth. The results of the SEM-PLS analysis revealed that aspects of environmental awareness and brand authenticity did not significantly influence the attitude of OFD customers, whereas, in this study, other aspects studied were found to have a significant effect. Customer attitude is a determining factor for intentional behaviour and electronic word of mouth. This study has supported the postulation of the importance of customer religiosity profiles and the Theory of Planned Behaviour

Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis

Publisher Copyright: © 2022, The Author(s).Background: Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. Results: To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N = 1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3–5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. Conclusions: Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.Peer reviewe

Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis

Funding GMP, PN, and CW are supported by NHLBI R01HL127564. GMP and PN are supported by R01HL142711. AG acknowledge support from the Wellcome Trust (201543/B/16/Z), European Union Seventh Framework Programme FP7/2007–2013 under grant agreement no. HEALTH-F2-2013–601456 (CVGenes@Target) & the TriPartite Immunometabolism Consortium [TrIC]-Novo Nordisk Foundation’s Grant number NNF15CC0018486. JMM is supported by American Diabetes Association Innovative and Clinical Translational Award 1–19-ICTS-068. SR was supported by the Academy of Finland Center of Excellence in Complex Disease Genetics (Grant No 312062), the Finnish Foundation for Cardiovascular Research, the Sigrid Juselius Foundation, and University of Helsinki HiLIFE Fellow and Grand Challenge grants. EW was supported by the Finnish innovation fund Sitra (EW) and Finska Läkaresällskapet. CNS was supported by American Heart Association Postdoctoral Fellowships 15POST24470131 and 17POST33650016. Charles N Rotimi is supported by Z01HG200362. Zhe Wang, Michael H Preuss, and Ruth JF Loos are supported by R01HL142302. NJT is a Wellcome Trust Investigator (202802/Z/16/Z), is the PI of the Avon Longitudinal Study of Parents and Children (MRC & WT 217065/Z/19/Z), is supported by the University of Bristol NIHR Biomedical Research Centre (BRC-1215–2001) and the MRC Integrative Epidemiology Unit (MC_UU_00011), and works within the CRUK Integrative Cancer Epidemiology Programme (C18281/A19169). Ruth E Mitchell is a member of the MRC Integrative Epidemiology Unit at the University of Bristol funded by the MRC (MC_UU_00011/1). Simon Haworth is supported by the UK National Institute for Health Research Academic Clinical Fellowship. Paul S. de Vries was supported by American Heart Association grant number 18CDA34110116. Julia Ramierz acknowledges support by the People Programme of the European Union’s Seventh Framework Programme grant n° 608765 and Marie Sklodowska-Curie grant n° 786833. Maria Sabater-Lleal is supported by a Miguel Servet contract from the ISCIII Spanish Health Institute (CP17/00142) and co-financed by the European Social Fund. Jian Yang is funded by the Westlake Education Foundation. Olga Giannakopoulou has received funding from the British Heart Foundation (BHF) (FS/14/66/3129). CHARGE Consortium cohorts were supported by R01HL105756. Study-specific acknowledgements are available in the Additional file 32: Supplementary Note. The views expressed in this manuscript are those of the authors and do not necessarily represent the views of the National Heart, Lung, and Blood Institute; the National Institutes of Health; or the U.S. Department of Health and Human Services.Peer reviewedPublisher PD

Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis

Abstract Background Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. Results To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N = 1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3–5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. Conclusions Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk

Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis

Funding Information: GMP, PN, and CW are supported by NHLBI R01HL127564. GMP and PN are supported by R01HL142711. AG acknowledge support from the Wellcome Trust (201543/B/16/Z), European Union Seventh Framework Programme FP7/2007–2013 under grant agreement no. HEALTH-F2-2013–601456 (CVGenes@Target) & the TriPartite Immunometabolism Consortium [TrIC]-Novo Nordisk Foundation’s Grant number NNF15CC0018486. JMM is supported by American Diabetes Association Innovative and Clinical Translational Award 1–19-ICTS-068. SR was supported by the Academy of Finland Center of Excellence in Complex Disease Genetics (Grant No 312062), the Finnish Foundation for Cardiovascular Research, the Sigrid Juselius Foundation, and University of Helsinki HiLIFE Fellow and Grand Challenge grants. EW was supported by the Finnish innovation fund Sitra (EW) and Finska Läkaresällskapet. CNS was supported by American Heart Association Postdoctoral Fellowships 15POST24470131 and 17POST33650016. Charles N Rotimi is supported by Z01HG200362. Zhe Wang, Michael H Preuss, and Ruth JF Loos are supported by R01HL142302. NJT is a Wellcome Trust Investigator (202802/Z/16/Z), is the PI of the Avon Longitudinal Study of Parents and Children (MRC & WT 217065/Z/19/Z), is supported by the University of Bristol NIHR Biomedical Research Centre (BRC-1215–2001) and the MRC Integrative Epidemiology Unit (MC_UU_00011), and works within the CRUK Integrative Cancer Epidemiology Programme (C18281/A19169). Ruth E Mitchell is a member of the MRC Integrative Epidemiology Unit at the University of Bristol funded by the MRC (MC_UU_00011/1). Simon Haworth is supported by the UK National Institute for Health Research Academic Clinical Fellowship. Paul S. de Vries was supported by American Heart Association grant number 18CDA34110116. Julia Ramierz acknowledges support by the People Programme of the European Union’s Seventh Framework Programme grant n° 608765 and Marie Sklodowska-Curie grant n° 786833. Maria Sabater-Lleal is supported by a Miguel Servet contract from the ISCIII Spanish Health Institute (CP17/00142) and co-financed by the European Social Fund. Jian Yang is funded by the Westlake Education Foundation. Olga Giannakopoulou has received funding from the British Heart Foundation (BHF) (FS/14/66/3129). CHARGE Consortium cohorts were supported by R01HL105756. Study-specific acknowledgements are available in the Additional file : Supplementary Note. The views expressed in this manuscript are those of the authors and do not necessarily represent the views of the National Heart, Lung, and Blood Institute; the National Institutes of Health; or the U.S. Department of Health and Human Services. Publisher Copyright: © 2022, The Author(s).Background: Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. Results: To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N = 1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3–5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. Conclusions: Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.Peer reviewe

MEMBANGUN SISTEM PENGUKURAN KINERJA PERUSAHAAN DENGAN MENGGUNAKAN FRAMEWORK BALANCED SCORECARD (Studi Kasus di Pos Express Semarang)

ABSTRAK Pos Express merupakan unit bisnis strategis dari PT. Pos Indonesia yang beroperasi di bidang jasa pengiriman express. Jasa yang dibuka di Pos Express adalah jasa pengiriman surat, dokumen, barang dan parcel. Pos Express termotivasi untuk melakukan perbaikan yang berkelanjutan terhadap pengukuran kinerja yang telah diterapkan sebelumnya yaitu pengukuran kinerja yang bertitik tolak pada ukuran keuangan dalam mengukur kinerja bisnisnya. Sebagaimana yang telah kita ketahui pengukuran kinerja berdasarkan ukuran finansial kurang dapat memberikan informasi yang akurat bagi perusahaan, karena ukuran finansial hanya menjelaskan berbagai peristiwa masa lalu. Pengukuran kinerja merupakan hal yang penting bagi perusahaan. Karena pengukuran kinerja berdasarkan aspek finansial tidak cukup mencerminkan kinerja perusahaan yang sesungguhnya, maka dikembangkanlah konsep Balanced Scorecard (BSC). Konsep BSC mengukur kinerja perusahaan dipandang dari empat perspektif, yaitu perspektif finansial, perspektif pelanggan, perspektif proses bisnis internal, dan perspektif pembelajaran dan pertumbuhan. Pada dasarnya, Balanced Scorecard merupakan penerjemahan dari strategi dan tujuan yang ingin dicapai suatu perusahaan dalam jangka panjang, yang kemudia diukur dan dimonitor secara terus-menerus. Dari hasil perancangan sistem pengukuran kinerja ini akhirnya diperoleh 35 Key Performance Indicators (KPI) yang dapat digunakan sebagai tolok ukur dalam kegiatan pengukuran kinerja Pos Express. Dalam proses perancangan ini juga menggunakan AHP sebagai tools untuk menyusun hierarkhi, melakukan pembobotan dan konsistensi. Dari KPI-KPI yang muncul, fokus Pos Express untuk perspektif finansial adalah tingkat pertumbuhan penjualan dan berkurangnya biaya-biaya produksi dengan bobot 0,372. Untuk perspektif customer tujuan strategis meningkatnya kepercayaan customer berfokus pada pengenalan masyarakat terhadap produk dengan bobot 0,481. untuk tujuan strategis meningkatnya kepuasan pelanggan berfokus pada banyaknya komplain pelanggan dengan bobot 0,625. Untuk persepktif proses bisnis internal khususnya proses collecting berfokus pada banyakan kesalahan input data dengan bobot 0,875. Untuk proses processing berfokus pada jumlah kesalahan pencatatan data kiriman dengan bobot 0,528. Untuk proses transporting berfokus pada banyaknya kiriman yang rusak dengan bobot 0,433. untuk proses delivery berfokus pada jumlah kiriman yang rusak, jumlah kesalahan tujuan pengiriman dan ketepatan waktu tempuh kiriman dengan bobot 0,284. Untuk persepktif pembelajaran dan pertumbuhan tujuan strategis meningkatnya kapabilitas dan komitmen karyawan berfokus pada kepuasan kerja karyawan dengan bobot 0,528. Untuk tujuan strategis akses ke informasi strategis berfokus pada kemudahan melakukan tracing kiriman dengan bobot 0,485

MEMBANGUN SISTEM PENGUKURAN KINERJA PERUSAHAAN DENGAN MENGGUNAKAN FRAMEWORK BALANCED SCORECARD (Studi Kasus di Pos Express Semarang)

Pos Express merupakan unit bisnis strategis dari PT. Pos Indonesia yang beroperasi di bidang jasa pengiriman express. Jasa yang dibuka di Pos Express adalah jasa pengiriman surat, dokumen, barang dan parcel. Pos Express termotivasi untuk melakukan perbaikan yang berkelanjutan terhadap pengukuran kinerja yang telah diterapkan sebelumnya yaitu pengukuran kinerja yang bertitik tolak pada ukuran keuangan dalam mengukur kinerja bisnisnya. Sebagaimana yang telah kita ketahui pengukuran kinerja berdasarkan ukuran finansial kurang dapat memberikan informasi yang akurat bagi perusahaan, karena ukuran finansial hanya menjelaskan berbagai peristiwa masa lalu. Pengukuran kinerja merupakan hal yang penting bagi perusahaan. Karena pengukuran kinerja berdasarkan aspek finansial tidak cukup mencerminkan kinerja perusahaan yang sesungguhnya, maka dikembangkanlah konsep Balanced Scorecard (BSC). Konsep BSC mengukur kinerja perusahaan dipandang dari empat perspektif, yaitu perspektif finansial, perspektif pelanggan, perspektif proses bisnis internal, dan perspektif pembelajaran dan pertumbuhan. Pada dasarnya, Balanced Scorecard merupakan penerjemahan dari strategi dan tujuan yang ingin dicapai suatu perusahaan dalam jangka panjang, yang kemudia diukur dan dimonitor secara terus-menerus. Dari hasil perancangan sistem pengukuran kinerja ini akhirnya diperoleh 35 Key Performance Indicators (KPI) yang dapat digunakan sebagai tolok ukur dalam kegiatan pengukuran kinerja Pos Express. Dalam proses perancangan ini juga menggunakan AHP sebagai tools untuk menyusun hierarkhi, melakukan pembobotan dan konsistensi. Dari KPI-KPI yang muncul, fokus Pos Express untuk perspektif finansial adalah tingkat pertumbuhan penjualan dan berkurangnya biaya-biaya produksi dengan bobot 0,372. Untuk perspektif customer tujuan strategis meningkatnya kepercayaan customer berfokus pada pengenalan masyarakat terhadap produk dengan bobot 0,481. untuk tujuan strategis meningkatnya kepuasan pelanggan berfokus pada banyaknya komplain pelanggan dengan bobot 0,625. Untuk persepktif proses bisnis internal khususnya proses collecting berfokus pada banyakan kesalahan input data dengan bobot 0,875. Untuk proses processing berfokus pada jumlah kesalahan pencatatan data kiriman dengan bobot 0,528. Untuk proses transporting berfokus pada banyaknya kiriman yang rusak dengan bobot 0,433. untuk proses delivery berfokus pada jumlah kiriman yang rusak, jumlah kesalahan tujuan pengiriman dan ketepatan waktu tempuh kiriman dengan bobot 0,284. Untuk persepktif pembelajaran dan pertumbuhan tujuan strategis meningkatnya kapabilitas dan komitmen karyawan berfokus pada kepuasan kerja karyawan dengan bobot 0,528. Untuk tujuan strategis akses ke informasi strategis berfokus pada kemudahan melakukan tracing kiriman dengan bobot 0,485. Kata Kunci : Balanced Scorecard (BSC), Key Performance Indicators (KPI), Analytical Hierarchy Process (AHP)

Nitrogen-fixing populations of Planctomycetes and Proteobacteria are abundant in surface ocean metagenomes

Nitrogen fixation in the surface ocean impacts global marine nitrogen bioavailability and thus microbial primary productivity. Until now, cyanobacterial populations have been viewed as the main suppliers of bioavailable nitrogen in this habitat. Although PCR amplicon surveys targeting the nitrogenase reductase gene have revealed the existence of diverse non-cyanobacterial diazotrophic populations, subsequent quantitative PCR surveys suggest that they generally occur in low abundance. Here, we use state-of-the-art metagenomic assembly and binning strategies to recover nearly one thousand non-redundant microbial population genomes from the TARA Oceans metagenomes. Among these, we provide the first genomic evidence for non-cyanobacterial diazotrophs inhabiting surface waters of the open ocean, which correspond to lineages within the Proteobacteria and, most strikingly, the Planctomycetes. Members of the latter phylum are prevalent in aquatic systems, but have never been linked to nitrogen fixation previously. Moreover, using genome-wide quantitative read recruitment, we demonstrate that the discovered diazotrophs were not only widespread but also remarkably abundant (up to 0.3% of metagenomic reads for a single population) in both the Pacific Ocean and the Atlantic Ocean northwest. Our results extend decades of PCR-based gene surveys, and substantiate the importance of heterotrophic bacteria in the fixation of nitrogen in the surface ocean

Blood and brain protein levels of ubiquitin-conjugating enzyme E2K <i>(UBE2K)</i> are elevated in individuals with schizophrenia

© 2019 Elsevier Ltd A number of recent studies have suggested the ubiquitin proteasome system (UPS) in schizophrenia is dysfunctional. The purpose of this study was to investigate UBE2K, a ubiquitin-conjugating (E2) enzyme within the UPS that has been associated with psychosis symptom severity, in the blood and brain of individuals with schizophrenia. Whole blood and erythrocytes from 128 (71 treatment-resistant schizophrenia, 57 healthy controls) individuals as well as frozen dorsolateral prefrontal cortex (DLPFC) and orbitofrontal cortex (OFC) post-mortem samples from 74 (37 schizophrenia, 37 controls) individuals were obtained. UBE2K gene expression was assayed in whole blood and DLPFC samples, whereas protein levels were assayed in erythrocytes and OFC samples. Elevated levels of UBE2K mRNA were observed in whole blood of individuals with schizophrenia (p = 0.03) but not in the DLPFC, while protein levels were raised in erythrocytes and the OFC (p < 0.001 and p = 0.002 respectively). Findings were not better explained by age, smoking, clozapine plasma levels or duration of illness. Although blood and brain samples were derived from independent samples, our findings suggest peripheral protein levels of UBE2K may serve as a surrogate of brain levels and further supports the notion of UPS dysfunction in schizophrenia. Future studies to determine the pathophysiological effects of elevated UBE2K protein levels in the brain of those with schizophrenia are warranted