24 research outputs found
Caries de la infancia temprana, demanda de atención odontológica y percepción parental de salud bucal en pre-escolares de riesgo social del Gran Mendoza
Get PDFLa caries de la infancia temprana es un problema de salud pública
que afecta con mayor agresividad a preescolares en riesgo social. El
objetivo del presente trabajo fue establecer las asociaciones existentes
entre estado dental, demanda de atención odontológica,
percepción de salud bucal y condición socio-económica en niños
preescolares del Gran Mendoza.
Material y método: la población estuvo constituida por escolares en
una muestra intencionada de 155 niños de nivel inicial residentes
en la provincia de Mendoza, pertenecientes al ámbito escolar urbano-
marginal. Se registraron los siguientes índices: ceod, CPOD,
ceos, CPOS, y sus componentes discriminados, ICDAS II, íNTC
(Bordoni, 1998) e índice de placa (Silness y Löe, 1967). Fue aplicado un cuestionario de percepción parental de salud bucal (ECOHIS)
y se realizaron encuestas estructuradas sobre tipología de la
demanda de atención odontológica y de condiciones socioeconómicas
(NBI). Fue determinada la distribución de frecuencias y los
intervalos de confianza para cada variable, las medidas de tendencia
central y dispersión y se realizaron comparaciones entre grupos
mediante la prueba de chi cuadrado con p=0.05.
Resultados: 85.8% de la muestra presentó experiencia de caries.
Para cada indicador se registraron los siguientes valores:
ceod+CPOD= 5.716 +/- 4.08; cd+CD =5,39+3.79; ed+PD= 0.25+-
0.72; y od+OD= 0.08+-0.34; ceos+CPOS=8.89+-8.39;
cs+CS=7.63+-6.25; es+PS= 1.23+-3.62; y os+OS= 0.12+0.46; IP=
1.04+-0.48; y INTC=5.65+-3.19. No se observaron diferencias significativas
al agrupar los niños por sexo. Al analizar la demanda de
atención bucal se observó que el 54,9% efectuó una consulta
odontológica, 25% en el último año y 74% en el subsistema de
salud pública. El análisis del cuestionario ECOHIS reveló que obtuvo
el máximo porcentaje de respuestas cuando se ordenó según la
categoría nunca. El único ítem que se relacionó con el estado dental
en forma estadísticamente significativa fue el dolor.
Conclusiones: Se observa alta prevalencia de caries dental sin que
la demanda de atención odontológica haya producido impacto
positivo sobre el estado dentario ni sobre la percepción de los
padres respecto de la salud bucal.Early childhood caries is a public health problem affecting pre-school social
risk children more aggressively. The aim of this study is to establish the
connection between dental condition, dental care demand, oral health
perception and socio-economic status between pre-school children in Mendoza.
MATERIAL AND METHOD: The intended sample is the school
population, consisting of 155 children-level residents, belonging to a
marginalized urban school environment in the province of Mendoza. The
following indexes were recorded: dmft, DMFT, dmfs, DMFS and its
differentiated components ICDAS II, INTC (Bordoni, 1998) and plaque
index (Silness and Löe, 1967). A questionnaire about parental perception
of oral health (ECOHIS) and structured surveys about the typology of the
demand for dental care and socioeconomic conditions (NBI) were conducted.The distribution of frequency, the confidence interval for each variable and
the central tendency and dispersion measures were determined. In addition,
comparisons among groups by means of the chi-square test, p = 0.05, were
also conducted. RESULTS: 85.8% of the sample had caries experience. For
each indicator the values were reported as follows: dmft + DMFT = 5716
+- 4.08; dt + DT = 5.39 +-3.79; mt +MT = 0.25 + -0.72, and ft +
FT = 0.08 + -0.34; dmfs + DMFS = 8.89 + -8.39; ds + DS = 7.63
+ -6.25; ms + MS = 1.23 + -3.62, and fs + FS = 0.12 +0.46; IP =
1.04 + -0.48, and INTC = 5.65 + -3.19. No significant differences were
observed when grouping children according to sex. When analyzing dental
care demand, it was found that 54.9% made a dental visit, 25% made it last
year, and 74%made it through the public health subsystem. ECOHIS
questionnaire analysis revealed that the highest percentage of responses were
obtained when the “never" category was included. Pain was the only
significant item related to dental condition statistically. CONCLUSIONS:
There is a high prevalence of dental caries. In addition, dental care demand
has not produced a positive impact neither on dental status nor in the oral
health parent perception.Fil: Fernández, Claudia Nélida.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Cambría Ronda, Salvador.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Vuoto, Elena Rosa.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Borjas, María Inés.
Universidad Nacional de Cuyo. Facultad de Odontologí
Frecuency and severity of early childhood caries in social risk children from mendoza
Get PDFEn este estudio descriptivo correlacional se tuvo como
objetivo el determinar la frecuencia y severidad de caries
según edad en niños entre 12-71 meses con riesgo social
del Gran Mendoza, y el impacto de las lesiones de esmalte
sobre estas variables. MÉTODO: Muestra: 404 niños de
12-71 meses de edad asistentes a SEOS del Gran Mendoza.
Indicadores: grupos de edad; ceod con 2 categorías:
para "c" según incluya lesión esmalte y dentina (c2-6)
o dentina (c4-6) según criterios de ICDAS II; y severidad
de caries. Se aplicó estadística paramétrica y no paramétrica
para analizar variablesindependientes, dispersión
y comparación de grupos; p≤0,05. RESULT ADOS: para
ceod2-6: 49.8% sin experiencia de caries, c 2,13±3.12; ei
0,14±0.77; o 0,3 ±0,27; ceod 2,30±3,38.Para ceod4-6:
64% sin experiencia de caries, c 1,21±2,35; ei 0,13±0.72;
o 0,3 ±0,27; ceod 1,37±2,59. Se encontró asociación estadísticamente
significativa para diferencias entre: ceod4-
6 y ceod2-6 y componentes discriminados;para categorías de severidad y los dos grupos de ceod; para severidad y
grupos de edad con aumento con la edad del componente
c y del ceod total en ceod2-6y ceod4-6. El porcentaje
de lesiones de caries de esmalte fue mayor en los
primeros años de vida, aumentando luego el de lesiones
dentinarias. La carga de caries de esmalte para toda la
población estudiada fue del 30,9%. CONCLUSIONES: en
los niños del Gran Mendoza evaluados el valor de ceod se
incrementa hacia el 2do y 3er año de vida, cuadruplicándose
hacia el 5to. Aumentan significativamente cuando
se incluyen lesiones de esmalte, con alta carga de las mismas
en los niños más pequeños.In this descriptive correlational study, the objective was to
determine the frequency and severity of caries according
to age in children between 12-71 months with social risk
of Greater Mendoza, and the impact of enamel lesions on
these variables. METHOD: Sample: 404 children aged 12-
71 months attending SEOS in Greater Mendoza, Indicators:
age groups; dmft with 2 categories: for "d" according
to include enamel and dentin (d2-6) or dentin (d4-6)
lesion according to ICDAS II criteria; and caries severity.
Parametric and non-parametric statistics were applied to
analyze independent variables, dispersion and comparison
of groups; P≤0.05. RESULTS: for dmft2-6: 49.8% without
caries experience, d 2.13 ± 3.12; m 0.14 ± 0.77; f 0.3 ±
0.27; dmft2-6 2.30 ± 3.38. For dmft4-6: 64% with no
caries experience, d 1.21 ± 2.35; m 0.13 ± 0.72; f 0.3
± 0.27; dmft4-61.37 ± 2.59. A statistically significant
association was found for differences between: dmft4-6
and dmft2-6 and discriminated components; for severity categories and the two groups of dmft; for severity and
age groups with an increase with age of the d component
and total dmft in dmft4-6 and dmft2-6. The percentage of
enamel caries lesions was higher in the first years of life,
then the dentine lesions increased. The enamel caries load
for the entire study population was 30.9%. CONCLUSIONS:
in the studied children of Greater Mendoza the value of
dmft increases in the 2nd and 3rd year of life, quadrupling
foward the 5th. They increase significantly when enamel
lesions were included, with high loading of enamel lesions
in younger children.Fil: Fernández, Claudia Nélida.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Vuoto, Elena Rosa.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Borjas, María Inés.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Cambría Ronda, Salvador.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Grilli, Silvia Mabel.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Arias, Susana Catalina.
Universidad Nacional de Cuyo. Hospital Universitario. Área de Odontología.Fil: Buttani, Natalia.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Calatayud, Laura.
Universidad Nacional de Cuyo. Hospital Universitario. Área de Odontología.Fil: Dolonguevich, Evelyn.
Universidad Nacional de Cuyo. Hospital Universitario. Área de Odontología.Fil: Martín, Marcela Cecilia.
Universidad Nacional de Cuyo. Hospital Universitario. Área de Odontología.Fil: Paez, Silvia.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Salinas, Daniela Esther.
Universidad Nacional de Cuyo. Hospital Universitario. Área de Odontología
Targeting Glioma Initiating Cells with A combined therapy of cannabinoids and temozolomide
Get PDFGlioblastoma multiforme (GBM) is the most frequent and aggressive type of brain tumor due, at least in part, to its poor response to current anticancer treatments. These features could be explained, at least partially, by the presence within the tumor mass of a small population of cells termed Glioma Initiating Cells (GICs) that has been proposed to be responsible for the relapses occurring in this disease. Thus, the development of novel therapeutic approaches (and specifically those targeting the population of GICs) is urgently needed to improve the survival of the patients suffering this devastating disease. Previous observations by our group and others have shown that Δ9-Tetrahydrocannabinol (THC, the main active ingredient of marijuana) and other cannabinoids including cannabidiol (CBD) exert antitumoral actions in several animal models of cancer, including gliomas. We also found that the administration of THC (or of THC + CBD at a 1:1 ratio) in combination with temozolomide (TMZ), the benchmark agent for the treatment of GBM, synergistically reduces the growth of glioma xenografts. In this work we investigated the effect of the combination of TMZ and THC:CBD mixtures containing different ratios of the two cannabinoids in preclinical glioma models, including those derived from GICs. Our findings show that TMZ + THC:CBD combinations containing a higher proportion of CDB (but not TMZ + CBD alone) produce a similar antitumoral effect as the administration of TMZ together with THC and CBD at a 1:1 ratio in xenografts generated with glioma cell lines. In addition, we also found that the administration of TMZ + THC:CBD at a 1:1 ratio reduced the growth of orthotopic xenografts generated with GICs derived from GBM patients and enhanced the survival of the animals bearing these intracranial xenografts. Remarkably, the antitumoral effect observed in GICs-derived xenografts was stronger when TMZ was administered together with cannabinoid combinations containing a higher proportion of CBD. These findings support the notion that the administration of TMZ together with THC:CBD combinations - and specifically those containing a higher proportion of CBD - may be therapeutically explored to target the population of GICs in GBM.This work has been funded by the PI15/00339 grant, integrated into the State Plan for R & D + I2013-2016 and funded by the Instituto de Salud Carlos III (ISCIII) (Spain) and the European Regional Development Fund (ERDF) and by grants from Spanish Ministry of Economy and Competitiveness (MINECO)/ISCIII and ERDF (PS09/01401; PI12/02248,to GV), GW Pharma Ltd. (UK), Comunidad de Madrid (Spain) (S2011/BMD-2308 to MG), Fundación Mutua Madrileña (Spain) (AP101042012 to GV), Fundació La Marató de TV3 (Spain) (201334031 to GV), Voices Against Brain Cancer (US), and donations by The Medical Cannabis Bike Tour Foundation (The Netherlands) and Jeff Ditchfield. Israel López-Valero was supported by a predoctoral P-FIS contract from Instituto de Salud Carlos III (ISCIII) and Cristina Sáiz was supported by a “Juan de la Cierva formación” contract of the Spanish Ministry of Economy and Competitiveness.S
Prevalencia de maloclusión en pre-escolares del Gran Mendoza, Mendoza, Argentina : su relación con hábitos persistentes
Get PDFEl presente trabajo desarrolló un estudio descriptivo a nivel regional en niños pre-escolares con los siguientes objetivos: -Establecer porcentajes de la población infantil pre-escolar (que asisten a jardín y pre-jardín) del Gran Mendoza, que usan mamadera y/o chupete.Determinar el tipo de maloclusión prevalente en la población pre-escolar del Gran Mendoza. -Establecer porcentajes de la población de niños pre-escolares que usan chupete y/o biberón y que presentan maloclusión dentaria.-Determinar el tipo de maloclusión prevalente en este subgrupo poblacional.
Conclusiones: El porcentaje de niños que persistieron en el hábito del chupete y/ o biberón en la población de alumnos de jardín y pre-jardín de las escuelas estudiadas es de casi el 20% de varones y mujeres promediado. No se pudo establecer una asociación entre la persistencia de éstos hábitos y el desarrollo de maloclusiones dentarias como se describe en la literatura. Esto podrfa relacionarse en gran medida con la biotipología, que por razones instrumentales no fue documentada. Se concluyó que es muy alto el porcentaje de la población escolar estudiada que presenta maloclusiones; 83,78% de niñas y el 76% de varones. Prevalecieron las clases I con mordida cerrada. La presencia de alteraciones transversales de la mordida fue de alrededor del 11 % para ambos sexos.This paper develops a descriptive and regional study in pre-scholars children. Objectives: -To stablish a percencentual of the children population in pre-scholar age in Grand Mendoza-Argentina, who are using milk bottles and/or pacifiers. -To determinate the type of dental malocclusion in this population. -To stablish a percentual in the subgroup who are using milk bottles and/or pacifiers, how many of them presents malocclusion and which type is the prevalent.
Conclusions: the percentual of pre-scholars children who are using milk bottles andlor pacifiers in the studied population is from almost the 20% in boys and girls in promedy. It wasnt found an association between the presence of these sucking habits and the development of dental malocclusions, as is referred in the literature. This can be related with the bio typology, wich had not been documented in this instance. The percentual of the studied population who presents dental malocclusion is very high: 83,78% of girls and 76% of boys. The prevalent malocclusion is c1ass I with deep bite. The presence of transversals alterations is about 11 % for booth sexes.Fil: Vuoto, Elena Rosa.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Prigione, Carlos.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Fernández, Claudia Nélida.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Cambría Ronda, Salvador.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Chade, Marisa.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Esquembre, Beatriz.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: De la Rosa, Guillermo.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Herreros, María de los Ángeles.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Anit, Pablo.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Martí, Sonia Ema.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Giamportone, María Verónica.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Valles, Pablo.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Villar, Ariel Dario.
Universidad Nacional de Cuyo. Facultad de OdontologíaFil: Carrió, María del Carmen.
Universidad Nacional de Cuyo. Facultad de Filosofía y LetrasFil: Solé, Verónica Beatriz.
Universidad Nacional de SaltaFil: Chia, Carla Lorena.Fil: Giuberti, Adriana.
Universidad Nacional de Cuyo. Facultad de Artes y Diseñ
Cytotoxicity of the Ascidian Cystodytes dellechiajei Against Tumor Cells and Study of the Involvement of Associated Microbiota in the Production of Cytotoxic Compounds
Get PDFMany cytotoxic compounds of therapeutic interest have been isolated from marine invertebrates, and some of them have been reported to be of microbial origin. Pyridoacridine alkaloids are the main compounds extracted from the ascidian Cystodytes dellechiajei. Here we describe the in vitro antiproliferative activity against different tumor cell lines of the ascidian extracts and provide some insights on the role of the microbial community associated with the tunicate in the production of these compounds. C. dellechiajei extracts showed remarkably high antiproliferative activity (IC50 ≤5 μg/mL) in human lung carcinoma A-549, colon adenocarcinoma H-116, pancreatic adenocarcinoma PSN-1 and breast carcinoma SKBR3 cell lines. Moreover, we found that the maximum activity was located in the tunic tissue of the colony, which harbours a microbial community. In order to ascertain the involvement of this community in the synthesis of the bioactive compounds different approachs that included culture and culture independent methods were carried out. We undertook a screening for antiproliferative activities of the bacterial isolates from the ascidian, as well as a comprative analysis of the cytotoxic activities and the microbial communities from two color morphs of the ascidian, green and blue. In addition, the changes of the antiproliferative activities and the composition of the microbial communities were studied from ascidians kept in aquaria and treated with antibiotics for one month. Our data obtained from the different experiments did not point out to bacteria as the source of the cytotoxic compounds, suggesting thus an ascidian origin
AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity
Get PDFMammalian development, adult tissue homeostasis and the avoidance of severe diseases including cancer require a properly orchestrated cell cycle, as well as error-free genome maintenance. The key cell-fate decision to replicate the genome is controlled by two major signalling pathways that act in parallel-the MYC pathway and the cyclin D-cyclin-dependent kinase (CDK)-retinoblastoma protein (RB) pathway(1,2). Both MYC and the cyclin D-CDK-RB axis are commonly deregulated in cancer, and this is associated with increased genomic instability. The autophagic tumour-suppressor protein AMBRA1 has been linked to the control of cell proliferation, but the underlying molecular mechanisms remain poorly understood. Here we show that AMBRA1 is an upstream master regulator of the transition from G1 to S phase and thereby prevents replication stress. Using a combination of cell and molecular approaches and in vivo models, we reveal that AMBRA1 regulates the abundance of D-type cyclins by mediating their degradation. Furthermore, by controlling the transition from G1 to S phase, AMBRA1 helps to maintain genomic integrity during DNA replication, which counteracts developmental abnormalities and tumour growth. Finally, we identify the CHK1 kinase as a potential therapeutic target in AMBRA1-deficient tumours. These results advance our understanding of the control of replication-phase entry and genomic integrity, and identify the AMBRA1-cyclin D pathway as a crucial cell-cycle-regulatory mechanism that is deeply interconnected with genomic stability in embryonic development and tumorigenesis
The Pseudokinase TRIB3 Negatively Regulates the HER2 Receptor Pathway and Is a Biomarker of Good Prognosis in Luminal Breast Cancer
Get PDFFrom MDPI via Jisc Publications RouterHistory: accepted 2021-10-19, pub-electronic 2021-10-22Publication status: PublishedFunder: Instituto de Salud Carlos III; Grant(s): PI18/00442Funder: European Commission; Grant(s): ITN-308 2016 721532Funder: Breast Cancer Now; Grant(s): 2012NovSP033Funder: Ministry of Economy, Industry and Competitiveness; Grant(s): RTI2018-094130-B-100Background: Tribbles pseudokinase 3 (TRIB3) has been proposed to both promote and restrict cancer generation and progression. However, the precise mechanisms that determine this dual role of TRIB3 in cancer remain to be understood. In this study we aimed to investigate the role of TRIB3 in luminal breast cancer, the most frequent subtype of this malignancy. Methods: We genetically manipulated TRIB3 expression in a panel of luminal breast cancer cell lines and analyzed its impact on cell proliferation, and the phosphorylation, levels, or subcellular localization of TRIB3 and other protein regulators of key signaling pathways in luminal breast cancer. We also analyzed TRIB3 protein expression in samples from luminal breast cancer patients and performed bioinformatic analyses in public datasets. Results: TRIB3 enhanced the proliferation and AKT phosphorylation in luminal A (HER2-) but decreased them in luminal B (HER2+) breast cancer cell lines. TRIB3 negatively regulated the stability of HER2 in luminal B breast cancer cell lines. TRIB3 expression was associated with increased disease-free survival and a better response to therapy in luminal breast cancer patients. Conclusions: Our findings support the exploration of TRIB3 as a potential biomarker and therapeutic target in luminal breast cancer
