EXPLAINING JAMMU AND KASHMIR CONFLICT UNDER INDIAN ILLEGAL OCCUPATION: PAST AND PRESENT

The Jammu and Kashmir conflict remains one of the oldest UN agenda items. However, despite the genuine plight and legitimate demand for the right to self-determination, Kashmiris in Indian Illegally Occupied Jammu and Kashmir continue to struggle for their lives and livelihood under Indian oppression and state-sponsored terrorism without respite. This paper, therefore, attempts to glean into the history of the conflict, which has a strong connection with the contemporary situation, while answering important questions: What circumstances compelled people to rush to Kashmir soon after the independence? How has India, over the decades, interpreted, misinterpreted, and manipulated world opinion in its favour? Why and how have the Muslim world and international community shown apathy towards the beleaguered Kashmiris? Lastly, can there be a way forward, especially after India’s illegal annexation of the UN declared disputed territory? It also highlights options available for a plausible solution to this humanitarian issue.   Bibliography Entry Malik, Salma, and Nasreen Akhtar. 2021. "Explaining Jammu and Kashmir Conflict under Indian Illegal Occupation: Past and Present." Margalla Papers 25 (1): 23-35

ROLE OF PAKISTANI FEMALE PEACEKEEPERS IN ENHANCING INTERNATIONAL HUMANITARIAN LAW: OPPORTUNITIES AND CHALLENGES

In recent years, there has been growing recognition of the effectiveness of an all-gender-inclusive approach to peacekeeping missions in conflict zones, as highlighted in the UNSCR 1325 on Women, Peace and Security. UN has acknowledged the importance of female peacekeepers as potential role models for girls in male-dominated societies and has taken steps to increase their numbers. Pakistan has been a significant contributor to UN Peacekeeping missions over the past six decades and has also achieved the target of deploying female soldiers in its contingent forces. This study uses qualitative methods, primarily focus group discussions, to evaluate the constructive impact of UN female peacekeepers and their potential to enhance passive compliance towards International Humanitarian Law in conflict zones. It also explores whether female peacekeepers make a unique contribution and investigates the existing gap in research on their performance and the challenges they face in the field. The study highlights the need for further research and support to address the social and cultural factors that continue to restrict the contribution of female peacekeepers, particularly from countries like Pakistan, where women face significant challenges in the security sector.   Bibliography Entry Malik, Salma, Ahmed Hasan Awan and Talha Ibrahim. 2023. "Role of Pakistani Female Peacekeepers in Enhancing International Humanitarian Law: Opportunities and Challenges." Margalla Papers 27 (1): 186-198

Smart parking guidance system using 360o camera and haar-cascade classifier on IoT system

Nowadays, smart parking guidance system is a crucial research for people’s convenience. The main objective of this research is to develop and analyze on a smart parking guidance system where current available system was compared to this new proposed system. Limited parking space has become serious issue since the number of Malaysia’s populations who are using car keep increasing. Some of the big companies, shopping malls and other public facilities already deployed a smart parking system on their building. However, there are still a lot of buildings that do not own it because the system required a lot of investment, where the huge parking areas need higher cost to install sensors on each parking lot available. The proposed smart parking guidance system in this research was depending on a 360° camera that was modified on raspberry pi camera module and 360o lens and Haar-Cascade classifier. The image and video processing was by Open CV and python program to detect the available parking space and cloud firebase was used to update data where users can access the parking space availability by android mobile phone specifically at a closed parking space. A single 360°camera was replaced several sensors and camera which was implemented on traditional smart parking system. An analysis was done on the performance of the system where it can detect the parking availability with 99.74% accuracy and which is far better than conventional system including reliability and cost for the parking space guidance system. © BEIESP

Effect of therapeutic interventions on skeletal growth & development in paediatric inflammatory bowel disease

Crohn’s disease (CD) is a chronic inflammatory bowel disease. Once considered rare in the paediatric population, it is recognized with increasing frequency among children of all ages. Approximately 20-30% of all patients with CD present when they are younger than 20 years. With its increasing recognition, CD has become one of the most important chronic diseases that affect children and adolescents. In addition to the common gastrointestinal (GI) symptoms (diarrhoea, rectal bleeding, and abdominal pain) children often experience growth retardation, pubertal delay, and bone demineralization. In these children, maintenance of skeletal health is a complex process that is influenced by a number of different mechanisms including steroid therapy, the disease process, nutritional status, endocrine status and the response of the body to inflammatory mediators. The recent introduction of biologic therapy that targets specific mediators of the proinflammatory process is a promising adjunct in the therapeutic management of the child with chronic inflammation. These drugs may also exert beneficial effects on the adverse effects of inflammation on growth and skeletal development. It is unclear whether these beneficial effects are due to improvement in overall disease or due to a direct ‘anti-cytokine’ effect at the level of the target tissue involved in growth and skeletal development. The hypothesis of this study was that the biologic therapy improves linear growth, puberty, bone health, body composition and muscle function in children with CD and this is associated with changes in the IGF-1 axis and markers of bone formation and bone resorption. Chapter 1 is an extensive literature review about the effects of biologic therapy on growth and skeletal development in paediatric patients with chronic inflammatory conditions particularly inflammatory bowel disease (IBD). The main aim of this review was to summarize and evaluate effects of inflammation and biologic therapy on growth and skeletal development in children with chronic inflammatory conditions and to explore the areas of interest for further research. Chapter 2 is the study about the growth in children receiving contemporary disease specific therapy in children with CD. The aim of this study was to assess the frequency of short stature and poor growth and their relationship to disease course and therapy in children with CD. Clinical records of all children with a confirmed diagnosis of CD, who were between 2yrs and 18yrs at the Royal Hospital for Sick Children, Glasgow were examined retrospectively. Data were collected at diagnosis, 1-yr, 2-yr and 3-yr after diagnosis and at maximum follow-up. The relationship of a number of factors including therapeutic modalities to two commonly used anthropometric markers of growth height velocity standard deviation scores (HVSDS) and change in height standard deviation scores (∆HtSDS) was examined. This study suggested that ∆HtSDS may be a more valid method of assessing and reporting longitudinal growth in children with chronic disease, particularly when there is a high prevalence of children of a peri-pubertal age. This study provides clear evidence that despite advances in therapy, short stature and slow growth continue to be encountered in a sub-group of children with CD. Chapter 3 is about the effect of Infliximab therapy on growth, puberty and disease activity in children with CD. The aim of this study was to assess growth, puberty, markers of disease and concomitant therapy over the six months prior to starting Infliximab and for the 6 and 12 months following treatment. Clinical records of all children with IBD who were started on Infliximab therapy between 2003 and 2008 at the Royal Hospital for Sick Children were examined retrospectively. This study has shown an average improvement of approximately 50% in HV in the 6 months after the initiation of Infliximab therapy which was further sustained for a further 6 months. Improvement in growth was found to be better in those children who were responders as compared to non-responders suggests that growth improved as a result of disease control. Improvement in growth was also observed in children who remained pre-pubertal and those who had never been on glucocorticoids (GC) compared to those who had been on GC. This study suggests that increase in height may not be simply due to progress in pubertal status or reduction in glucocorticoid dose. Chapter 4 is about the effect of Adalimumab therapy on growth in paediatric patients with CD. This is the one and only world wide multicentre study that adequately assess the effect of Adalimumab on linear growth in children with CD. The aim of this study was to assess the effect of Adalimumab therapy on growth, puberty and disease activity over the 6 months prior to and 6 months after starting Adalimumab treatment in children with CD. This study provides evidence that Adalimumab is associated with improvement in short term linear growth in children with CD who enter remission but not in those who do not. It is also more likely to happen in children who are on immunosuppression and those in early puberty but seems to be relatively independent of steroid use. These findings suggest that growth improves as a result of several interrelated factors, including improved disease control. It was also interesting to note that the growth response to Adalimumab varied dependent on the reason for discontinuing Infliximab; those who had an allergic reaction to Infliximab fared best. Chapter 5 is Longitudinal observational prospective study of changes in physical growth, IGF-1 axis, bone health, body composition, muscle function and disease activity at baseline (BL), 2 weeks (2wk), 6 weeks (6wk), 6 and 12 months (6M & 12M) following biologic therapy in paediatric patients with CD. The aim of this longitudinal observational prospective study was to assess changes in physical growth, puberty, IGF-1 axis, bone health; body composition and muscle function following biologic therapy in paediatric patients with CD. Patients either newly diagnosed or patients with long-lasting disease in clinical relapse, who started treatment with biologic therapy as part of their standard clinical management, were recruited. A non significant improvement was observed in both ∆HtSDS and HVcms/yr at 12M as compared to BL. Individually, the majority of the children experienced improvement in clinical activity and improvement of the systemic inflammatory markers. A significant increase in biomarker of bone formation bone specific alkaline phosphatase (BALP) and a non-significant increase in a biomarker of bone resorption cross-linked c-terminal telopeptides (CTX-1) was observed from BL to 12M. This observation suggests the beneficial effect of biologic therapy on bone formation. This study showed a significant change in fat mass (FM (kg) in paediatric patients with CD following biologic therapy an effect that has not been reported extensively in previously published studies. A significant change in both fat free mass (FFM (kg) and fat free mass index (FFMI(kg/m²) shows that the treatment with anti-TNF-α therapy also had a significant impact on fat mass accrual. This is the first study that charts the effect of biologic therapy on changes in lower limb muscle function using jumping mechanography in paediatric patients with CD. A non significant change was observed in jump height (m), V-max (m/s), EFI (%), efficiency % from BL to 12M following biologic therapy and a significant increase in both F-max (kN), and P-max (kW) at 12M. Despite the fact that the increase in efficiency % of the movement was not significant but however, the change was likely to be through improvements in jump height and velocity thereby indicating higher muscular flexibility. These data are suggestive of an effect of biologic therapy on lower limb muscle function through improvements in the mechanical efficiency of the muscle. Thus, it is possible that the better muscle function is mediated through the effect of biologic therapy on muscle mass. Peripheral quantitative computed tomography (PQCT) for tibia and radius both indicated significant increase in fat and muscle cross sectional area (Fat-CSA (mm²) and Mus-CSA (mm²). Moreover, tibia pQCT also indicated significant change in stress strain index (SSI (mm³) a surrogate marker of bone strength, at 12M of therapy. No significant changes were observed in total body, lumbar spine (L2-L4), proximal femur and femoral neck bone mineral density (BMD (g/cm²), insulin like growth factor-1 (IGF-1(ng/ml), insulin like growth factor binding proteins (IGFBP-3 (ng/ml), IGFBP-2 (ng/ml) and acid labile subunits (ALS (ng/ml) following biologic therapy. In conclusion, these prospective studies of growth, bone health, body composition and muscle function have suggested that biologic therapy in children with CD has a beneficial effect on muscle mass and muscle function and which can be observed over the first year of therapy for conclusive explanation of these changes these data needs to be adjusted for body size. These positive changes are also associated with an increase in bone turnover where the change in bone formation is much greater than bone resorption. These favourable effects on bone health were not accompanied by marked changes in BMD as assessed by DXA but did show some beneficial effects on pQCT assessed SSI, a surrogate marker of bone strength. It is possible that controlling disease activity with biologic therapy may positively outweigh the effects of CD on growth, body composition, muscle function and bone health. The results of this preliminary study need to be confirmed in a larger group of children. An improved understanding of the effect of biologic therapy may improve future therapy directed at promoting growth and skeletal development in a diverse group of children. Further studies are required to understand the duration of the window of opportunity during which linear growth, bone and muscle mass in children with CD can be optimised. Chapter 6 focuses on the main findings of this thesis and discusses potential limitations of this methodology, and outlines some important and interesting extensions for future research in paediatric patients with CD following biologic therapy

Phasing out the general nursing diploma programme at Aga khan university school of nursing Karachi, Pakistan

Aga Khan University was established as medical complex in 1980 and in 1983 received charter as first private university in Pakistan. Thus nursing education moved under the umbrella of a university which allowed starting under graduate and graduate degree education and in light of global trends in nursing profession and staffing and financial impact of nursing education, 2010 was the last intake of university\u27s founding programme; general nursing diploma. Concerns; male vs female nurses and taking nursing education as an easy route to enter in higher education resulting in unattained staffing require deliberations by stake holder including Pakistan Nursing Council

An analysis of adherence to the World Health Organisation guidelines pertaining to drug promotional literature by pharmaceutical firms

Background: Pharmaceutical companies used Drug Promotion Literatures (DPLs) as a major tool to advertise their new products. World Health Organization (WHO) has set some guidelines for promotion of drug literature. Thus, this study aimed to evaluate various DPLs for their accuracy and credibility as per WHO ethical criteria.Methods: This was an observational and cross-sectional study. Total 100 drugs advertisements published in various medical journals were collected from the library of the college. Advertisements were selected based on inclusion and exclusion criteria. The selected advertisements were evaluated based on the WHO ethical criteria for drug promotion.Results: From 100 advertisements, 73 were single drug whereas 27 were fixed drug combinations. Antimicrobials (16%) were the most promoted advertisements. Only 28% of the advertisements carried references to support their claim. Out of which majority (91.78%) were from journal articles. The generic name, brand name, names of active ingredients, manufacturer, distributor and dosage regimen were mentioned in majority of advertisements. Drug interactions (12%), contraindications (22%), precautions (24%) and side effects (22%) were least mentioned in the advertisements.Conclusions: It was observed that none of the advertisement followed all the guidelines laid down by WHO. Pharmaceutical companies should follow ethical regulatory measures to promote their product in various journals. The regulatory authority must ensure the pharmaceutical companies to follow ethical guidelines for publishing various drug promotional literatures

BK Virus Load Associated with Serum Levels of sCD30 in Renal Transplant Recipients

Background. Rejection is the main drawback facing the renal transplant operations. Complicated and overlapping factors, mainly related to the immune system, are responsible for this rejection. Elevated serum levels of sCD30 were frequently recorded as an indicator for renal allograft rejection, while BV virus is considered as one of the most serious consequences for immunosuppressive treatment of renal transplant recipients (RTRs). Aims. This study aimed to determine the association of BK virus load with serum levels of sCD30 in RTRs suffering from nephropathy. Patients and Methods. A total of 50 RTRs with nephropathy and 30 age-matched apparently healthy individuals were recruited for this study. Serum samples were obtained from each participant. Real-time PCR was used to quantify BK virus load in RTRs serum, while ELISA technique was employed to estimate serum levels of sCD30. Results. Twenty-two percent of RTRs had detectable BKV with mean viral load of 1.094E + 06 ± 2.291E + 06. RTRs showed higher mean serum level of sCD30 (20.669±18.713 U/mL) than that of controls (5.517±5.304 U/mL) with significant difference. BK virus load had significant positive correlation with the serum levels of sCD30 in RTRs group. Conclusion. These results suggest that serum levels of sCD30 could be used as an indicator of BK viremia, and accordingly the immunosuppressive regime should be adjusted

Antihypertensive Activity of Aqueous-Methanol Extract of Berberis Orthobotrys Bien Ex Aitch in Rats

Purpose: To investigate the hypotensive potential of Berberis orthobotrys Bien Ex Aitch (Family: Berberidaceae) in both normotensive and hypertensive rats.Methods: Aqueous-methanol (70:30) extract of Berberis orthobotrys at doses of 25, 50, 75 and 100 mg/kg was evaluated for its effect on blood pressure and heart rate using non-invasive blood pressure measuring apparatus. After initial screening, 100 mg/kg dose that produced a maximum effect was selected for the antihypertensive study. Median lethal dose (LD50) and sub-chronic toxicity of the extract were also determined. Various biochemical parameters and organ weight were measured usingstandard procedures.Results: The extract produced a significant (p < 0.01) decrease in systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP) and heart rate of normotensive rats at all test doses with maximum effect at 100 mg/kg. Similarly, a significant antihypertensive and negative chronotropic effect was observed in both hypertensive models. LD50 of the extract was 200 mg/kg in mice. The extract also exhibited a reduction (p < 0.05) in serum alanine transaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), triglycerides and low density lipoprotein (LDL) levels while a significant (p < 0.05) increase in high density lipoproteins (HDL) level was observed.Conclusion: It seems that the aqueous-methanol extract of Berberis orthobotrys possesses active compounds which may be responsible for the antihypertensive and negative chronotropic effects in rats.Keywords: Berberis orthobotrys, Antihypertensive, Egg feed diet, Blood lipid

Long non-coding RNAs and their targets as potential biomarkers in breast cancer.

Breast cancer is among the lethal types of cancer with a high mortality rate, globally. Its high prevalence can be controlled through improved analysis and identification of disease-specific biomarkers. Recently, long non-coding RNAs (lncRNAs) have been reported as key contributors of carcinogenesis and regulate various cellular pathways through post-transcriptional regulatory mechanisms. The specific aim of this study was to identify the novel interactions of aberrantly expressed genetic components in breast cancer by applying integrative analysis of publicly available expression profiles of both lncRNAs and mRNAs. Differential expression patterns were identified by comparing the breast cancer expression profiles of samples with controls. Significant co-expression networks were identified through WGCNA analysis. WGCNA is a systems biology approach used to elucidate the pattern of correlation between genes across microarray samples. It is also used to identify the highly correlated modules. The results obtained from this study revealed significantly differentially expressed and co-expressed lncRNAs and their cis- and trans-regulating mRNA targets which include RP11-108F13.2 targeting TAF5L, RPL23AP2 targeting CYP4F3, CYP4F8 and AL022324.2 targeting LRP5L, AL022324.3, and Z99916.3, respectively. Moreover, pathway analysis revealed the involvement of identified mRNAs and lncRNAs in major cell signalling pathways, and target mRNAs expression is also validated through cohort data. Thus, the identified lncRNAs and their target mRNAs represent novel biomarkers that could serve as potential therapeutics for breast cancer and their roles could also be further validated through wet labs to employ them as potential therapeutic targets in future