Haz, reflexiona y aprende

El presente trabajo de Fin de Máster en Profesorado de E.S.O y Bachillerato, F.P. y Enseñanzas de Idiomas, Artísticas y Deportivas, dentro de la especialidad de Economía “Do, Think & Learn; Haz, Reflexiona y Aprende” ya que guarda estrecha relación con la reflexión del Máster, de los prácticums y de las actividades desarrolladas

Durability Assessment of a Plasma-Polymerized Coating with Anti-Biofilm Activity against L. monocytogenes Subjected to Repeated Sanitization

Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).[EN] Biofilm formation on food-contact surfaces is a matter of major concern causing food safety and spoilage issues to this sector. The aim of this study was to assess the durability of the anti-biofilm capacity of a plasma-polymerized coating composed of a base coating of (3-aminopropyl) triethoxysilane (APTES) and a functional coating of acrylic acid (AcAc). Coated and uncoated AISI 316 stainless steel (SS) plates were subjected to five sanitization cycles with sodium hypochlorite (0.05%) and peracetic acid (0.5%). The effectiveness of the coating for the inhibition of multi-strain Listeria monocytogenes biofilm formation was confirmed using a three-strain cocktail, which was grown on the SS plates at 12◦ C for 6 days. Compared to the uncoated SS, relative biofilm productions of 14.6% on the non-sanitized coating, 27.9% on the coating after sanitization with sodium hypochlorite, and 82.3% on the coating after sanitization with peracetic acid were obtained. Morphological and physicochemical characterization of the coatings suggested that the greater anti-biofilm effectiveness after sanitization with sodium hypochlorite was due to the high pH of this solution, which caused a deprotonation of the carboxylic acid groups of the functional coating. This fact conferred it a strong hydrophilicity and negatively charged its surface, which was favorable for preventing bacterial attachment and biofilm formation.SIThis publication is based upon work from COST Action CA19110—PlAgri, supported by COST (European Cooperation in Science and Technology-www.cost.eu). XPS tests were conducted by the Advanced Microscopy Laboratory (LMA) of The Institute of Nanoscience of Aragón (INA), University of Zaragoza. The authors are thankful to the LMA-INA for the access to their equipment and their expertise. The AFM images were taken by the Central Research Support Service (SCAI) of the University of Málaga (UMA). The author P. Fernández-Gómez is grateful to Junta de Castilla y León and the European Social Fund (ESF) for awarding her a predoctoral grant (BOCYL-D-15122017-4). The author M. Oliveira is in receipt of a Juan de la Cierva contract IJC2018-035523-I awarded by the Spanish Ministry of Science, Innovation, and Universities MCIN/AEI/10.13039/501100011033. The author E. Sainz-García, as researcher of the University of La Rioja, is thankful to the postdoctoral training program funded by the Plan Propio of the University of La Rioja. The authors I. Muro-Fraguas and A. Sainz-García are thankful to the program of pre-doctoral contracts for the training of research staff that is funded by the University of La Rioja.This study is part of the Research, Development and Innovation projects AGL2017-82779- C2-R and PID2020-113658RB-C2, funded by MCIN/AEI/10.13039/501100011033 and by ERDF “A way to make Europe”

Ciencia, Ficción y Diseño: saberes tridimensionales y seres post robóticos

3er Coloquio Fortalecimiento de los Colectivos de Docencia.Presentación que muestran el proceso del diseño: la narrativa de la imagen, la imaginación y creatividad, y el proceso de diseño. De esta manera se valora mucho el proceso de diseño, la correcta documentación, se reconoce el método empleado, como parte fundamental del proceso de diseño. Se considera principalmente lo valioso del aprendizaje por sobre los productos y resultados como el objetivo del proceso de enseñanza aprendizaje

Organoleptic characterization of wines in contact with oak wood fragments immersed in plasma activated water (PAW)

Oak barrels are a valuable material for wine ageing, although their difficult cleaning and disinfection favours microbiological contamination causing wine quality depreciation. Atmospheric pressure cold plasma is a suitable technique to reduce microbiota, but there is little research on its impact on the nutritional and sensory characteristics of foods. The aim of this study was to analyse the organoleptic characteristics of red wines in contact with plasma-activated waters (PAW) treated wood, of different origins and toasting levels. These red wines were compared with others that had been in contact with two types of control wood; some immersed in distilled water and others subjected to sulphur dioxide combustion. The results showed that oak wood treated with PAW did not cause defects at the olfactory and gustatory level of the red wines, which presented good harmony, sufficient body and balanced fruity and spicy notes, sometimes even superior to those described for red wines in contact with control wood (sulphited or submerged in distilled water). Therefore, the treatment of the wood with PAW did not have a negative impact on the sensory quality of the wines, regardless of the origin and toasting of the wood

Biomarkers Associated with Regorafenib First-Line Treatment Benefits in Metastatic Colorectal Cancer Patients: REFRAME Molecular Study

© 2021 by the authors.First-line treatment with regorafenib in frail metastatic colorectal cancer (mCRC) patients has shown some benefit. To accurately identify such patients before treatment, we studied blood biomarkers and primary tumor molecules. We unveiled serum microRNAs (miRNAs), single-nucleotide polymorphisms (SNPs) in angiogenic-related genes, and Notch 1 expression as biomarkers associated with response or toxicity. MicroRNA array profiling and genotyping of selected SNPs were performed in the blood of fragile mCRC patients treated with regorafenib. Notch 1 and CRC-associated miRNA expression was also analyzed in tumors. High levels of miR-185-5p in serum, rs7993418 in the vascular endothelial growth factor receptor 1 (VEGFR1) gene, and Notch 1 expression in biopsies were associated with a favorable response to treatment. Serum levels of miR-126-3p and miR-152-3p and tumor expression of miR-92a-1-5p were associated with treatment toxicity, particularly interesting in patients exhibiting comorbidities, and high levels of miR-362-3p were associated with asthenia. Additionally, several miRNAs were associated with the presence of metastasis, local recurrence, and peritoneal metastasis. Besides, miRNAs determined in primary tumors were associated with tumor-node-metastasis (TNM) staging. The rs2305948 and rs699947 SNPs in VEGFR2 and VEGFA, respectively, were markers of poor prognosis correlating with locoregional relapse, a higher N stage, and metastatic shedding. In conclusion, VEGF and VEGFR SNPs, miRNAs, and Notch 1 levels are potential useful biomarkers for the management of advanced CRC under regorafenib treatment.Financial support for this research was provided by Bayer Hispania, which had no role in the design of the study; the collection, analysis, and interpretation of data; and the writing of the manuscript.Peer reviewe