Characterization and inhibition studies of an α-carbonic anhydrase from the endangered sturgeon species Acipenser gueldenstaedti.

An α-carbonic anhydrase (CA, EC 4.2.1.1) was purified and characterized kinetically from erythrocytes of the sturgeon Acipenser gueldenstaedti, an endangered species. The sturgeon enzyme (AgCA) showed kinetic parameters for the CO(2) hydration reaction comparable with those of the human erythrocytes enzyme hCA II, being a highly active enzyme, whereas its esterase activity with 4-nitrophenyl acetate as substrate was lower. Sulphonamide inhibitors (acetazolamide, sulphanilamide) strongly inhibited AgCA, whereas metal ions (Ag(+), Zn(2+), Cu(2+) and Co(2+)) were weak, millimolar inhibitors. Several widely used pesticides (2,4-dichlorophenol, dithiocarbamates, parathion and carbaryl) were also assayed as inhibitors of this enzyme. The dithiocarbamates were low micromolar AgCA inhibitors (IC(50) of 16-18 μM), whereas the other pesticides inhibited the enzyme with IC(50)s in the range of 102-398 μM. The wide use of dithiocarbamate pesticides may be one of the factors enhancing the vulnerability of this sturgeon species to pollutants

Prediction Of Problematic Internet Use By Attachment In University Students

Aim of this research is to examine the predictive power of attachment style on problematic internet use among university students. Participants of study consist of 481 university students (230 girls). Results indicate that there is a negative correlation between secure attachment style and social benefit/social comfort and there is a positive correlation between preoccupied attachment style and social benefit/social comfort which is a sub-dimension of problematic internet use. Considering predictive power of attachment on problematic internet use, results show that preoccupied, secure and dismissing attachment styles are significant predictors of social benefit/social comfort. Results and comments for the future studies on problematic internet use and attachment were discussed in general

The Effect of Vascular Graft and Human Umbilical Cord Blood-Derived CD34+ Stem Cell on Peripheral Nerve Healing

AIM: There are many trials concerning peripheral nerve damage causes and treatment options. Unfortunately, nerve damage is still a major problem regarding health, social and economic issues. On this study, we used vascular graft and human cord blood derived stem cells to find an alternative treatment solution to this problem. MATERIAL AND METHODS: We used 21 female Wistar rats on our study. They were anesthetized with ketamine and we studied right hind limbs. On Group 1, we did a full layer cut on the right sciatic nerve. On Group 2, we did a full layer cut on the right sciatic nerve, and we covered synthetic vascular graft on cut area. On Group 3, we did a full layer cut on right sciatic nerve, and we covered the area with stem cell applied vascular graft. RESULTS: At the end of postoperative 8. weeks, we performed EMG on the rats. When we compared healthy and degenerated areas as a result of EMG, we found significant amplitude differences between the groups on healthy areas whereas there was no significant difference on degenerated  areas between the groups. Then we re-opened the operated area again to reveal the sciatic nerve cut area, and we performed electron microscope evaluation. On the stem cell group, we observed that both the axon and the myelin sheet prevented degeneration. CONCLUSION: This study is a first on using synthetic vascular graft and cord blood derived CD34+ cells in peripheral nerve degeneration. On  the  tissues  that  were  examined  with  electron  microscope,  we  observed  that  CD34+  cells  prevented  both  axonal  and  myelin  sheath degeneration. Nerve tissue showed similar results to the control group, and the damage was minimal

Recent Developments in Magnetic Sensing

Measurement systems must have some properties such as sensitivity, accuracy, stability and low power consumption. New sensor types are continuously being developed according to new needs of applications besides improving measurement systems. In this study, recently developed magnetic sensors are searched. Fundamentals of new magnetic sensor types, their advantages and application areas are presented and compared in a table to form a guide for magnetic sensor users

Investigation of effect of design and operating parameters on acoustophoretic particle separation via 3D device-level simulations

In the present study, a 3D device-level numerical model is implemented via finite element method to assess the effects of design and operating parameters on the separation performance of a microscale acoustofluidic device. Elastodynamic equations together with electromechanical coupling at the piezoelectric actuators for the stress field within the solid parts, Helmholtz equation for the acoustic field within fluid, and Navier-Stokes equations for the fluid flow are coupled for the simulations. Once the zero-acoustic and flow fields are obtained, the trajectories of the particles are obtained by employing point-particle approach. The particle trajectories are simulated for many particles with different sizes released from random initial locations. Separation performances of the different cases are evaluated based on described metrics such as purity, yield, percentage of particle stuck in the channel, the force acting on the particles, residence time and separation parameter

Treatment and risk factors of hepatic veno-occlusive disease after pediatric hematopoietic stem cell transplantation: A single-center experience

45th Annual Meeting of the European-Society-for-Blood-and-Marrow-Transplantation (EBMT) -- MAR 24-27, 2019 -- Frankfurt, GERMANYWOS: 000487707800323…European Soc Blood & Marrow Transplanta

Stroke in sickle cell anemia: A case report

22nd International Congress on Thrombosis -- OCT 06-09, 2012 -- Nice, FRANCEWOS: 000310349200189

Acute Cyanosis Developing after the Placement of Central Venous Catheter: Methemoglobinemia Related to Local Prilocaine

WOS: 000381858000003Introduction: Methemoglobinemia is a rare serious hematological disorder, which is one of the causes of cyanosis in childhood, requiring emergency treatment. It may occur in congenital and acquired circumstances. Congenital methemoglobinemia can be a result of a genetic defect in erythrocyte metabolism and hemoglobin structure. Case Report: Acquired methemoglobinemia occurs in individuals exposed to various oxidant drugs and toxins, but exposure to medication is the most common cause of methemoglobinemia. Local anesthetics such as prilocaine, bupivicaine, and lidocaine are widely used in clinical practice and are common oxidant drugs that cause methemoglobinemia. The diagnosis of methemoglobinemia should be considered in patients presenting with hypoxia and cyanosis. Severity of symptoms correlates with the existing methemoglobin level. Conclusion: In this report, we presented an infant case in which methemoglobinemia developed after the administration of prilocaine prior to the placement of a central venous catheter

The moderating effect of gender in the relationship between narcissism and selfie-posting behavior

This study contributes to the cyberpsychology literature by investigating the moderating effect of gender in the relationship between narcissism and selfie posting behavior. A demographic questionnaire, 13-item selfie-posting scale developed and validated in the present study, and Narcissistic Personality Inventory (NPI-16) were used to collect data. An EFA was conducted to test the factor structure of scales based on data collected from 179 young adults (age 18-27 years). A CFA was then conducted using data from 269 university students (age 18-32 years) to test construct validity of the scales. A correlation analysis was employed to test the correlations between narcissism and selfie posting behavior. The results indicated that the correlations between attitudes, intentions, behaviors, and narcissism are significant for men, but not for women. The results also indicated gender differences in online behavior with women spending more time on social media and selfie-posting

Evaluation of antioxidant activity of bee products of different bee races in Turkey

YILDIZ, OKTAY/0000-0003-0436-682X; Sahin, Huseyin/0000-0002-6018-1494WOS: 000479121700002Bee products with high bioactive properties have a very important place in human nutrition. Biochemical properties of these products such as honey, pollen, and propolis vary according to flora, season, bee race, and production methods. In this study, the biological activities of honey, bee pollen, and propolis samples from four bee races in Turkey (Apis mellifera caucasica, Apis mellifera anatoliaca, Apis mellifera syriaca, and Apis mellifera carnica) were examined. Besides the determining of total polyphenol and total flavonoid contents, the antioxidant capacity was investigated by three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activities, CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power). The rank order of antioxidant potencies was as follows in the four bee races: propolis > pollen > honey. The polyphenol content of the products was evaluated and Apis mellifera syriaca for honey, Apis mellifera anatoliaca for pollen, and Apis mellifera carnica for propolis were found higher than the others. When bee races were compared, it was seen that flora is a more effective factor than bee race for antioxidant capacity.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [214Z211]This study was supported by TUBITAK with Project No. 214Z211