35 research outputs found
Biosynthesis of unnatural glycolipids possessing diyne moiety in the acyl chain in the green sulfur photosynthetic bacterium Chlorobaculum tepidum grown by supplementation of 10,12-heptadecadiynic acid
AbstractUnnatural glycolipids possessing the diyne moiety in their acyl groups were successfully biosynthesized in the green sulfur photosynthetic bacterium Chlorobaculum (Cba.) tepidum by cultivation with supplementation of 10,12-heptadecadiynic acid. Monogalactosyldiacylglycerol (MGDG) and rhamnosylgalactosyldiacylglycerol (RGDG) esterified with one 10,12-heptadecadiynic acid were primarily formed in the cells, and small amounts of glycolipids esterified with the two unnatural fatty acids can also be detected. The relative ratio of these unnatural glycolipids occupied in the total glycolipids was estimated to be 49% based on HPLC analysis using a evaporative light scattering detector. These results indicate that the acyl groups in glycolipids, which play important roles in the formation of extramembranous antenna complexes called chlorosomes, can be modified in vivo by cultivation of green sulfur photosynthetic bacteria with exogenous synthetic fatty acids. Visible absorption and circular dichroism spectra of Cba. tepidum containing the unnatural glycolipids demonstrated the formation of chlorosomes, indicating that the unnatural glycolipids in this study did not interfere with the biogenesis of chlorosomes
Effect of Nutrient Inputs on Water Quality Change and Phytoplankton Growth in Atsumi Bay
Eutrophication in an estuary occurs as an effect of the enrichment of nutrient inputs from rivers. This condition has become one of the most common environmental issues experienced around the globe and especially in Japan. Atsumi Bay is a eutrophic coastal area in Japan. The objective of this research was to analyze the influences of nutrient inputs from the Umeda River into Atsumi Bay on pre- and post-rainfall water quality conditions. This study was conducted from July to October 2010. The results showed a decrease of surface salinity after rainfall indicating that huge freshwater inputs had overlaid the surface layer of Atsumi Bay rather than the bottom layer. Moreover, post-rainfall conditions showed an increase of chlorophyll a as an effect of phytoplankton growth, followed by an increase of particulate nutrients. On the other hand, dissolved nutrients decreased due to uptake by phytoplankton and dilution by freshwater
Whole-Body Cortisol Concentrations and Ontogeny of Aggressive Behavior in Yellowtail (Seriola quinqueradiataTemminck & Schlegel; Carangidae)
Ontogenetic changes in whole-body immunoreactive cortisol concentrations (IRC) and aggressive behavior were examined in yellowtailSeriola quinqueradiata(Temminck & Schlegel; Carangidae). Baseline IRC significantly increased during the transition from larval to juvenile stage and was correlated with the onset of aggressive behavior. Handled fish (13.1 ± 2.6 ng/g tissue) showed an IRC level about three times higher than unhandled fish (4.7 ± 1.4 ng/g tissue), indicating that whole-body immunoreactive cortisol level may be an indicator of stress in juvenile yellowtails. Behaviorally subordinate fish (8.6 ± 1.6 ng/g tissue,n = 4) showed IRC levels significantly higher than dominant fish (0.6 ± 0.3 ng/g tissue, n = 4). Whole-body immunoreactive cortisol levels may thus reflect stress and social status in juvenile yellowtails, and the inverse relationship between social rank and IRC may result from agonistic interactions
Determination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria <i>Rhodoblastus acidophilus</i>, <i>Rhodobacter sphaeroides</i>, and <i>Phaeospirillum molischianum</i> by Extraction of Bacteriochlorophyll <i>a</i>
Removal OF B800 Bacteriochlorophyll A from Two Structure-Determined Light-Harvesting Proteins 2 in Purple Photosynthetic Bacteria
Systematic Analysis of the Demetalation Kinetics of Zinc Chlorophyll Derivatives Possessing Different Substituents at the 3‑Position: Effects of the Electron-Withdrawing and Electron-Donating Strength of Peripheral Substituents
Removal of the central metal from chlorophyll (Chl) molecules
is biologically important in terms of production of the primary electron
acceptors in photosystem-II photosynthetic reaction centers and the
early stage in Chl degradation. The physicochemical properties on
demetalation of chlorophyllous pigments are useful in the understanding
of such reaction mechanisms in photosynthetic organisms. Here we analyzed
the demetalation kinetics of a series of Zn-Chl derivatives with a
systematic variation in the electron-withdrawing and -donating substituents
at the 3-position of the chlorin macrocycle under acidic conditions
to elucidate thoroughly the substitution effects on the demetalation
properties of chlorophyllous pigments. Dehydrogenation of the aliphatic
group (CH<sub>2</sub>CH<sub>3</sub> → CHCH<sub>2</sub> → CCH) at the 3-position slowed the removal of the
central zinc from the chlorin macrocycle. The gradual decrease in
the demetalation rate constants of the three zinc chlorins originates
from differences in the electron-withdrawing strength of the ethyl,
vinyl, and ethynyl groups directly linked to the chlorin π macrocycle.
Reduction of the 3<sup>1</sup>-carbonyl groups significantly increased
the demetalation rate constants, and the relative ratios of the demetalation
rate constants of the zinc chlorins possessing a carbonyl group to
those possessing the corresponding hydroxy group were analogous in
the cases of 3-formyl- and 3-acetyl-zinc chlorins. The demetalation
rate constants of the seven Zn-Chl derivatives possessing various
electron-withdrawing and -donating groups exhibited good correlation
with the Hammett σ parameters of the 3-position substituents