A comparative study on the structure of English abstracts of Persian and English medical journals

زمینه و هدف: امروزه مقاله نویسی علمی اهمیت خاصی پیدا کرده است و بهترین راه به اشتراک گذاشتن دستاوردهای علمی بنظر میرسد به شرط آنکه معیارهای تعیین شده مرتبط با زبان مقاله نویسی رعایت گردد. هدف از این تحقیق شناسایی انحرافات ساختاری چکیده های ترجمه انگلیسی چاپ شده در مجلات فارسی زبان ایرانی و مقایسه آنها با چکیده هایی است که از ابتدا به زبان انگلیسی نوشته شده و در مجلات بین المللی پزشکی به چاپ رسیده اند. روش بررسی: در این مطالعه توصیفی- تحلیلی تعداد 64 چکیده (32 چکیده از مجلات ایرانی و 32 چکیده از مجلات بین المللی) بر اساس مدل پیشنهادی Swales مورد تجزیه و تحلیل و مقایسه قرار گرفت. بررسی دقیق تری هم بر اساس مدلCARS (Create A Research Space) پیشنهاد شده توسطSwales در قسمت مقدمه این چکیده ها انجام شد. توالی مقدمه، مواد و روش ها، یافته ها و نتیجه گیری (IMRC=Introduction, Methods, Results, Conclusion) به عنوان قسمتهای ساختاری جهت بررسی در نظر گرفته شد. داده ها به کمک آزمون های آماری کای اسکوئر و آزمون دقیق فیشر تجزیه و تحلیل شد. یافته ها: یافته ها نشان داد در حالی که چکیده های ترجمه شده همگی توالی IMRC را رعایت کرده اند، در چکیده های اصیل انگلیسی زبان این توالی کاملاً رعایت نشده است. در قسمت مقدمه چکیده ها، اختلاف معنی داری در نگارش بیان هدف تحقیق وجود داشت که در چکیده های ترجمه شده بیشتر به چشم می خورد (001/0

Rhetorical Variation in Medical Article Abstracts Written in English and Persian

The present study aimed at finding structural variations in the translated abstracts from Persian into English and comparing them with abstracts originally written in English and published in international journals in the area of medical sciences. To do this, 64 medical article abstracts (32 in Iranian journals, 32 in international journals) were analyzed and compared on the basis of Swales’ model (1990). More detailed analysis was done in the Introduction unit regarding CARS model (Swales, 1990) and also language features of each unit were identified. The IMRC (Introduction, Methods, Results, Conclusion) sequence was considered as the structural conventions for the analysis. The results showed that in terms of structural units, there was a significant difference in using the Methods unit between the two groups of abstracts (p= 0.002). Some variations were observed in the Introduction unit and language features. The data revealed that the translated abstracts from Persian into English in research medical articles meet the determined criteria for scientific writing while the original ones often ignore the criteria, although they are linguistically superior to the original English one

Traumatic brain injury and peripheral immune suppression: primer and prospectus

Nosocomial infections are a common occurrence in patients following traumatic brain injury (TBI) and are associated with an increased risk of mortality, longer length of hospital stay and poor neurological outcome. Systemic immune suppression arising as a direct result of injury to the central nervous system (CNS) is considered to be primarily responsible for this increased incidence of infection, a view strengthened by recent studies that have reported novel changes in the composition and function of the innate and adaptive arms of the immune system post TBI. However, our knowledge of the mechanisms that underlie TBI-induced immune suppression is equivocal at best. Here, after summarising our current understanding of the impact of TBI on peripheral immunity and discussing CNS-mediated regulation of immune function, we propose roles for a series of novel mechanisms in driving the immune suppression that is observed post TBI. These mechanisms, which have never been considered before in the context of TBI-induced immune paresis include the CNS-driven emergence into the circulation of myeloid derived suppressor cells and suppressive neutrophil subsets, and the release from injured tissue of nuclear and mitochondria-derived damage associated molecular patterns. Moreover, in an effort to further our understanding of the mechanisms that underlie TBI-induced changes in immunity, we pose throughout the review a series of questions, which if answered would address a number of key issues such as establishing whether manipulating peripheral immune function has potential as a future therapeutic strategy by which to treat and/or prevent infections in the hospitalised TBI patient

Application of the upper lip catch test for airway evaluation in edentulous patients: An observational study

Background: Application of upper lip catch test (ULCT) for airway evaluation in edentulous patients. Methods: This research is an evaluation of a clinical diagnostic test in edentulous patients prior to operation. Five hundred eighty eight edentulous patients in a referral university hospital, between March 2008 and June 2011 scheduled for elective surgery under general anesthesia were enrolled. Those unable to open the mouth and those with pharyngo-laryngeal pathology were excluded. ULCT was assessed and compared with Cormack-Lehane grading as a gold standard for airway evaluation. Results: A high negative predictive value of 99.4% was a notable finding for the ULCT. The results also showed a high specificity (89.4%), high sensitivity (75.0%) and a high accuracy (89.3%) in a comparatively higher range for the ULCT. Conclusion: The ULCT proved to be a useful predictor for airway assessment in edentulous patients in this setting. Further studies are needed to reconfirm its validity in other ethnic groups

Plasmid-based CRISPR-Cas9 system efficacy for introducing targeted mutations in CD81 gene of MDA-MB-231 cell line

Introduction. Breast cancer has been represented a challenging issue worldwide as it is one of the major leading causes of death among women. CD81 gene, a member of the tetraspanin protein family, has been associated with the development of human cancers. Genome editing technologies, particularly the CRISPR-Cas9 system, have shown rapid progress in gene function studies. In this study, we aimed to evaluate the ability of the CRISPR-Cas9 plasmid-based system to modify specific regions of the CD81 gene in the MDA-MB-231 breast cancer cell line. Materials and methods. Using bioinformatics database search, four different single guide RNAs (sgRNAs) totarget exon 3 and exon 5 of the CD81 gene were designed. The intended sgRNAs sequences were cloned into the expression plasmid pSpCas9(BB)-2A-GFP (PX458) bearing sgRNA scaffold backbone, Cas9, and EGFP coding sequences, which was confirmed by colony PCR and sequencing. Transfection efficiency was determined by fluorescence microscopy and flow cytometry analysis. Gene editing efficiency was measured qualitatively and quantitatively using the T7E1 and TIDE software, respectively. Results. Our data show that expression constructs were successfully introduced into MDA-MB-231 cells with an acceptable transfection efficiency. Two sgRNAs that were afforded to introduce significant mutations in their target regions were detected by TIDE software (p-value < 0.05). To the best of our knowledge, CD81 gene editing in these cells has been investigated for the first time in this study using the CRISPR/Cas9 technique. Conclusions. Taken together, our data show that the CRISPR-Cas9 system can change the genomic sequence in the target area of MDA-MB-231 cells. Along with previous studies, we propose forethought when using T7E1-based quantitative indel estimates, as comparing activities of multiple gRNAs with the T7E1 assay may lead to inaccurate conclusions. Instead, estimating non-homologous end-joining events (NHEJ) by Sanger sequencing and subsequent TIDE analysis is recommended

MRI-based synthetic CT in the detection of knee osteoarthritis: Comparison with CT

Magnetic resonance Imaging is the gold standard for assessment of soft tissues; however, X-ray-based techniques are required for evaluating bone-related pathologies. This study evaluated the performance of synthetic computed tomography (sCT), a novel MRI-based bone visualization technique, compared with CT, for the scoring of knee osteoarthritis. sCT images were generated from the 3T T1-weighted gradient-echo MR images using a trained machine learning algorithm. Two readers scored the severity of osteoarthritis in tibiofemoral and patellofemoral joints according to OACT, which enables the evaluation of osteoarthritis, from its characteristics of joint space narrowing, osteophytes, cysts and sclerosis in CT (and sCT) images. Cohen's κ was used to assess the interreader agreement for each modality, and intermodality agreement of CT- and sCT-based scores for each reader. We also compared the confidence level of readers for grading CT and sCT images using confidence scores collected during grading. Inter-reader agreement for tibiofemoral and patellofemoral joints were almost-perfect for both modalities (κ = 0.83–0.88). The intermodality agreement of osteoarthritis scores between CT and sCT was substantial to almost-perfect for tibiofemoral (κ = 0.63 and 0.84 for the two readers) and patellofemoral joints (κ = 0.78 and 0.81 for the two readers). The analysis of diagnosis confidence scores showed comparable visual quality of the two modalities, where both are showing acceptable confidence levels for scoring OA. In conclusion, in this single-center study, sCT and CT were comparable for the scoring of knee OA.</p

Statistical shape model of the talus bone morphology: A comparison between impinged and nonimpinged ankles

Diagnosis of ankle impingement is performed primarily by clinical examination, whereas medical imaging is used for severity staging and treatment guidance. The association of impingement symptoms with regional three-dimensional (3D) bone shape variaties visible in medical images has not been systematically explored, nor do we know the type and magnitude of this relation. In this cross-sectional case-control study, we hypothesized that 3D talus bone shape could be used to quantitatively formulate the discriminating shape variations between ankles with impingement from ankles without impingement, and we aimed to characterize and quantify these variations. We used statistical shape modeling (SSM) methods to determine the most prevalent modes of shape variations that discriminate between the impinged and nonimpinged ankles. Results of the compactness and parallel analysis test on the statistical shape model identify 8 prominent shape modes of variations (MoVs) representing approximately 78% of the total 3D variations in the population of shapes, among which two modes captured discriminating features between impinged and nonimpinged ankles (p value of 0.023 and 0.042). Visual inspection confirms that these two shape modes, capturing abnormalities in the anterior and posterior parts of talus, represent the two main bony risk factors in anterior and posterior ankle impingement. In conclusion, in this research using SSM we have identified shape MoVs that were found to correlate significantly with bony ankle impingement. We also illustrated potential guidance from SSMs for surgical planning.Biomaterials & Tissue Biomechanic