The relationship between realistic and free color selection in the visual expression of preschool-aged children

Završni rad Odnos realističnog i slobodnog odabira boje u likovnom izrazu djece rane predškolske dobi sadrži teorijsko znanje o boji te istraživanje izvedeno putem praktične aktivnosti u dječjem vrtiću „Đakovo“. Uzorak se sastojao od 20 djece, 10 radova iz mlađe skupine te 10 radova iz starije skupine. Kvalitativnom analizom dječjih radova provjerile su se postavljene hipoteze, kako bi se provjerilo koliko će realno djeca likovno izraziti boje na crtežu s motivom viđenog voća i povrća te utvrditi razlike u prikazu s obzirom na dob. Rezultati ovoga istraživanja ispunili su očekivanja; analizirajući dječje radova dobila sam potvrdu da su djeca u mlađoj dobnoj skupini od 3-4 godine sposobna prikazati stvaran i realan prikaz boja motiva na crtežu kao i starija dobna skupina od 5-6 godina.Final work the relationship between realistic and free color selection in the artistic expression of early pre-school children includes theoretical knowledge of color and research carried out through practical activities in the Đakovo pre-school The sample consisted of 20 children, 10 works from the younger group and 10 works from the older group. Qualitative analysis of child labor, hypotheses are being tested to determine how well the art-colored paint on a drawing with the motif of seen fruit and vegetables will determine the difference in age view. The results of this research have met expectations; analyzing children's work, I got the confirmation that children in the younger age group of 3-4 years are able to present a real and realistic picture of paint motifs on the drawing as well as an older age group of 5-6 years

The relationship between realistic and free color selection in the visual expression of preschool-aged children

Završni rad Odnos realističnog i slobodnog odabira boje u likovnom izrazu djece rane predškolske dobi sadrži teorijsko znanje o boji te istraživanje izvedeno putem praktične aktivnosti u dječjem vrtiću „Đakovo“. Uzorak se sastojao od 20 djece, 10 radova iz mlađe skupine te 10 radova iz starije skupine. Kvalitativnom analizom dječjih radova provjerile su se postavljene hipoteze, kako bi se provjerilo koliko će realno djeca likovno izraziti boje na crtežu s motivom viđenog voća i povrća te utvrditi razlike u prikazu s obzirom na dob. Rezultati ovoga istraživanja ispunili su očekivanja; analizirajući dječje radova dobila sam potvrdu da su djeca u mlađoj dobnoj skupini od 3-4 godine sposobna prikazati stvaran i realan prikaz boja motiva na crtežu kao i starija dobna skupina od 5-6 godina.Final work the relationship between realistic and free color selection in the artistic expression of early pre-school children includes theoretical knowledge of color and research carried out through practical activities in the Đakovo pre-school The sample consisted of 20 children, 10 works from the younger group and 10 works from the older group. Qualitative analysis of child labor, hypotheses are being tested to determine how well the art-colored paint on a drawing with the motif of seen fruit and vegetables will determine the difference in age view. The results of this research have met expectations; analyzing children's work, I got the confirmation that children in the younger age group of 3-4 years are able to present a real and realistic picture of paint motifs on the drawing as well as an older age group of 5-6 years

Whole genome sequencing characterization of Slovenian carbapenem-resistant Klebsiella pneumoniae, including OXA-48 and NDM-1 producing outbreak isolates

Objectives The first hospital outbreak of carbapenemase-producing Enterobacteriaceae in Slovenia occurred in 2014-2016. Whole genome sequencing was used to analyse the population of carbapenem-resistant Klebsiella pneumoniae collected in Slovenia in 2014-2017, including OXA-48 and/or NDM-1 producing strains from the outbreak. Methods A total of 32 K. pneumoniae isolates were analysed using short-read sequencing. Multilocus sequence typing and core genome multi-locus sequence typing were used to infer genetic relatedness. Antimicrobial resistance markers, virulence factors, plasmid content and wzi types were determined. Long-read sequencing was used for six isolates for detailed analysis of plasmids and their possible transmission. Results Overall, we detected 10 different sequence types (STs), the most common being ST437 (40.6%). Isolates from the initial outbreak belonged to ST437 (12/16) and ST147 (4/16). A second outbreak of four ST15 isolates was discovered. A new ST (ST3390) and two new wzi types (wzi-556, wzi-559) were identified. blaOXA-48 was found in 17 (53.1%) isolates, blaNDM-1 in five (15.6%), and a combination of blaOXA-48/NDM-1 in seven (21.9%) isolates. Identical plasmids carrying blaOXA-48 were found in outbreak isolates sequenced with long-read sequencing technology. Conclusions Whole genome sequencing of Slovenian carbapenem-resistant K. pneumoniae isolates revealed multiple clusters of STs, two of which were involved in the first hospital outbreak of carbapenem producing K. pneumoniae in Slovenia. Transmission of the plasmid carrying blaOXA-48 between two STs was likely to have occurred. A previously unidentified second outbreak was also discovered, highlighting the importance of whole genome sequencing in detection and/or characterization of hospital outbreaks and surveillance of drug-resistant bacterial clones

Expression, purification and immunological characterization of recombinant protein fragment from SARS-CoV-2

Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production, suitable for serological diagnosis. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified by immobilized metal ion affinity chromatography and strong cation exchange chromatography after which it was analyzed by Mass and CD spectrometry and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.Book of Abstract

Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species

In this study, a cost-effective sandwich ELISA test, based on polyclonal antibodies, for routine quantification SARS-CoV-2 nucleocapsid (N) protein was developed. The recombinant N protein was produced and used for the production of mice and rabbit antisera. Polyclonal N protein-specific antibodies served as capture and detection antibodies. The prototype ELISA has LOD 0.93 ng/mL and LOQ 5.3 ng/mL, with a linear range of 1.52–48.83 ng/mL. N protein heat pretreatment (56 °C, 1 h) decreased, while pretreatment with 1% Triton X-100 increased analytical ELISA sensitivity. The diagnostic specificity of ELISA was 100% (95% CI, 91.19–100.00%) and sensitivity was 52.94% (95% CI, 35.13–70.22%) compared to rtRT-PCR (Ct < 40). Profoundly higher sensitivity was obtained using patient samples mostly containing Wuhan-similar variants (Wuhan, alpha, and delta), 62.50% (95% CI, 40.59 to 81.20%), in comparison to samples mostly containing Wuhan-distant variants (Omicron) 30.00% (6.67–65.25%). The developed product has relatively high diagnostic sensitivity in relation to its analytical sensitivity due to the usage of polyclonal antibodies from two species, providing a wide repertoire of antibodies against multiple N protein epitopes. Moreover, the fast, simple, and inexpensive production of polyclonal antibodies, as the most expensive assay components, would result in affordable antigen tests

Ružička days : International conference 16th Ružička Days “Today Science – Tomorrow Industry” : Proceedings

Proceedings contains articles presented at Conference divided into sections: open lecture (1), chemical analysis and synthesis (3), chemical and biochemical engineering (8), food technology and biotechnology (8), medical chemistry and pharmacy (3), environmental protection (11) and meeting of young chemists (2)

The relationship between realistic and free color selection in the visual expression of preschool-aged children

Završni rad Odnos realističnog i slobodnog odabira boje u likovnom izrazu djece rane predškolske dobi sadrži teorijsko znanje o boji te istraživanje izvedeno putem praktične aktivnosti u dječjem vrtiću „Đakovo“. Uzorak se sastojao od 20 djece, 10 radova iz mlađe skupine te 10 radova iz starije skupine. Kvalitativnom analizom dječjih radova provjerile su se postavljene hipoteze, kako bi se provjerilo koliko će realno djeca likovno izraziti boje na crtežu s motivom viđenog voća i povrća te utvrditi razlike u prikazu s obzirom na dob. Rezultati ovoga istraživanja ispunili su očekivanja; analizirajući dječje radova dobila sam potvrdu da su djeca u mlađoj dobnoj skupini od 3-4 godine sposobna prikazati stvaran i realan prikaz boja motiva na crtežu kao i starija dobna skupina od 5-6 godina.Final work the relationship between realistic and free color selection in the artistic expression of early pre-school children includes theoretical knowledge of color and research carried out through practical activities in the Đakovo pre-school The sample consisted of 20 children, 10 works from the younger group and 10 works from the older group. Qualitative analysis of child labor, hypotheses are being tested to determine how well the art-colored paint on a drawing with the motif of seen fruit and vegetables will determine the difference in age view. The results of this research have met expectations; analyzing children's work, I got the confirmation that children in the younger age group of 3-4 years are able to present a real and realistic picture of paint motifs on the drawing as well as an older age group of 5-6 years

Early Lyme Borreliosis in Patients Treated with Tumour Necrosis Factor-Alfa Inhibitors

The study evaluated the course and outcome of erythema migrans in patients receiving tumour necrosis factor-alpha (TNF-&alpha;) inhibitors. Among 4157 adults diagnosed with erythema migrans in the period 2009&ndash;2018, 16 (2.6%) patients were receiving TNF-&alpha; inhibitors (adalimumab, infliximab, etarnecept, golimumab), often in combination with other immunosuppressants, for rheumatic (13 patients) or inflammatory bowel (three patients) disease. Findings in this group were compared with those in 32 sex- and age-matched immunocompetent patients diagnosed with erythema migrans in the same years. In comparison with the control group, the immunocompromised patients had a shorter incubation period (7 vs. 14 days; p = 0.0153), smaller diameter of erythema migrans (10.5 vs. 15.5 cm; p = 0.0014), and more frequent comorbidities other than immune-mediated diseases (62.5% vs. 25%, p = 0.0269), symptoms/signs of disseminated Lyme borreliosis (18.8% vs. 0%, p = 0.0324), and treatment failure (25% vs. 0%, p = 0.0094). After retreatment with an antibiotic, the clinical course of Lyme borreliosis resolved. Continuing TNF inhibitor treatment during concomitant borrelial infection while using identical approaches for antibiotic treatment as in immunocompetent patients resulted in more frequent failure of erythema migrans treatment in patients receiving TNF inhibitors. However, the majority of treatment failures were mild, and the course and outcome of Lyme borreliosis after retreatment with antibiotics was favourable

Early Lyme borreliosis in patients treated with tumour necrosis factor-alfa inhibitors

The study evaluated the course and outcome of erythema migrans in patients receiving tumour necrosis factor-alpha (TNF-α) inhibitors. Among 4157 adults diagnosed with erythema migrans in the period 2009–2018, 16 (2.6%) patients were receiving TNF-α inhibitors (adalimumab, infliximab, etarnecept, golimumab), often in combination with other immunosuppressants, for rheumatic (13 patients) or inflammatory bowel (three patients) disease. Findings in this group were compared with those in 32 sex- and age-matched immunocompetent patients diagnosed with erythema migrans in the same years. In comparison with the control group, the immunocompromised patients had a shorter incubation period (7 vs. 14 daysp = 0.0153), smaller diameter of erythema migrans (10.5 vs. 15.5 cmp = 0.0014), and more frequent comorbidities other than immune-mediated diseases (62.5% vs. 25%, p = 0.0269), symptoms/signs of disseminated Lyme borreliosis (18.8% vs. 0%, p = 0.0324), and treatment failure (25% vs. 0%, p = 0.0094). After retreatment with an antibiotic, the clinical course of Lyme borreliosis resolved. Continuing TNF inhibitor treatment during concomitant borrelial infection while using identical approaches for antibiotic treatment as in immunocompetent patients resulted in more frequent failure of erythema migrans treatment in patients receiving TNF inhibitors. However, the majority of treatment failures were mild, and the course and outcome of Lyme borreliosis after retreatment with antibiotics was favourable