99 research outputs found
Volume-based referral for cardiovascular procedures in the United States: a cross-sectional regression analysis
BACKGROUND: We sought to estimate the numbers of patients affected and deaths avoided by adopting the Leapfrog Group's recommended hospital procedure volume minimums for coronary artery bypass graft (CABG) surgery and percutaneous coronary intervention (PCI). In addition to hospital risk-adjusted mortality standards, the Leapfrog Group recommends annual hospital procedure minimums of 450 for CABG and 400 for PCI to reduce procedure-associated mortality. METHODS: We conducted a retrospective analysis of a national hospital discharge database to evaluate in-hospital mortality among patients who underwent PCI (n = 2,500,796) or CABG (n = 1,496,937) between 1998 and 2001. We calculated the number of patients treated at low volume hospitals and simulated the number of deaths potentially averted by moving all patients to high volume hospitals under best-case conditions (i.e., assuming the full volume-associated reduction in mortality and the capacity to move all patients to high volume hospitals with no related harms). RESULTS: Multivariate adjusted odds of in-hospital mortality were higher for patients treated in low volume hospitals compared with high volume hospitals for CABG (OR 1.16, 95% CI 1.10–1.24) and PCI (OR 1.12, 95% CI 1.05–1.20). A policy of hospital volume minimums would have required moving 143,687 patients for CABG and 87,661 patients for PCI from low volume to high volume hospitals annually and prevented an estimated 619 CABG deaths and 109 PCI deaths. Thus, preventing a single death would have required moving 232 CABG patients or 805 PCI patients from low volume to high volume hospitals. CONCLUSION: Recommended hospital CABG and PCI volume minimums would prevent 728 deaths annually in the United States, fewer than previously estimated. It is unclear whether a policy requiring the movement of large numbers of patients to avoid relatively few deaths is feasible or effective
Prediction of Obesity in Children at 5 years: A Cohort Study
Objective To examine determinants of moderate and severe obesity in children at 5 years of age. Methodology A prospective cohort of mothers were enrolled at first antenatal visit, and interviewed shortly after delivery, at 6 months and 5 years. Detailed health, psychological and social questionnaires were completed at each phase by mothers, and child health questionnaires at 6 months and 5 years. At 5 years 4062 children were assessed physically, the Peabody Picture Vocabulary Test administered and mothers completed a modified Child Behaviour Checklist. Moderate obesity was defined as BMI between 85th and 94th percentiles inclusively, and severe obesity as a BMI greater than the 94th percentile. Results Independent predictors of severe obesity at 5 years were birthweight, female gender, maternal BMI and paternal BMI. Moderate obesity at 5 years was predicted by birthweight, paternal BMI and sleeplessness at 6 months, while small for gestational age (SGA) status and feeding problems at 6 months were protective factors for moderate obesity. Obesity was not associated with problems of language comprehension or behaviour. Conclusions Findings of this study suggest that biological rather than psychosocial factors are the major determinants of obesity at 5 years
Genomic analysis of 6,000-year-old cultivated grain illuminates the domestication history of barley
Research achievements in plant resistance to insect pests of cool season food legumes
Plant resistance to at least 17 field and storage insect pests of cool season food legumes has been identified. For the most part, this resistance was located in the primary gene pools of grain legumes via conventional laboratory, greenhouse, and field screening methods. The use of analytical techniques (i.e., capillary gas chromatography) to characterize plant chemicals that mediate the host selection behavior of pest insects offers promise as a new, more rapid way to differentiate between insect-resistant and susceptible plant material. Examples of research achievements in mechanisms of resistance and host-plant resistance within the context of integrated control programs are discussed. Accelerating the development and subsequent releases of insect-resistant cultivars to pulse farmers requires more involvement from interdisciplinary teams of plant breeders, entomologists, plant pathologists, plant chemists, molecular biologists, and other scientist
Transcriptome profiling of ontogeny in the acridid grasshopper Chorthippus biguttulus
Acridid grasshoppers (Orthoptera:Acrididae) are widely used model organisms
for developmental, evolutionary, and neurobiological research. Although there
has been recent influx of orthopteran transcriptomic resources, many use
pooled ontogenetic stages obscuring information about changes in gene
expression during development. Here we developed a de novo transcriptome
spanning 7 stages in the life cycle of the acridid grasshopper Chorthippus
biguttulus. Samples from different stages encompassing embryonic development
through adults were used for transcriptomic profiling, revealing patterns of
differential gene expression that highlight processes in the different life
stages. These patterns were validated with semi-quantitative RT-PCR. Embryonic
development showed a strongly differentiated expression pattern compared to
all of the other stages and genes upregulated in this stage were involved in
signaling, cellular differentiation, and organ development. Our study is one
of the first to examine gene expression during post-embryonic development in a
hemimetabolous insect and we found that only the fourth and fifth instars had
clusters of genes upregulated during these stages. These genes are involved in
various processes ranging from synthesis of biogenic amines to chitin binding.
These observations indicate that post-embryonic ontogeny is not a continuous
process and that some instars are differentiated. Finally, genes upregulated
in the imago were generally involved in aging and immunity. Our study
highlights the importance of looking at ontogeny as a whole and indicates
promising directions for future research in orthopteran development
Chickpea
The narrow genetic base of cultivated chickpea warrants systematic collection,
documentation and evaluation of chickpea germplasm and particularly wild
Cicer species for effective and efficient use in chickpea breeding programmes.
Limiting factors to crop production, possible solutions and ways to overcome
them, importance of wild relatives and barriers to alien gene introgression and
strategies to overcome them and traits for base broadening have been discussed.
It has been clearly demonstrated that resistance to major biotic and abiotic
stresses can be successfully introgressed from the primary gene pool
comprising progenitor species. However, many desirable traits including high
degree of resistance to multiple stresses that are present in the species
belonging to secondary and tertiary gene pools can also be introgressed by
using special techniques to overcome pre- and post-fertilization barriers.
Besides resistance to various biotic and abiotic stresses, the yield QTLs have
also been introgressed from wild Cicer species to cultivated varieties. Status
and importance of molecular markers, genome mapping and genomic tools
for chickpea improvement are elaborated. Because of major genes for various
biotic and abiotic stresses, the transfer of agronomically important traits into
elite cultivars has been made easy and practical through marker-assisted
selection and marker-assisted backcross. The usefulness of molecular markers
such as SSR and SNP for the construction of high-density genetic maps of
chickpea and for the identification of genes/QTLs for stress resistance, quality
and yield contributing traits has also been discussed
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