Patellar tendon in vivo regional strain with varying knee angle

Purpose: Descriptive data on the aspects of site specific in vivo tendon strain with varying knee joint angle are non-existent. The present study determines and compares surface and deep layer strain of the patellar tendon during isometric contractions across a range of knee joint angles. Methods: Male participants (age 22.0 ± 3.4) performed ramped isometric knee extensions at knee joint angles of 900, 700, 500 and 300 of flexion. Strain patterns of the anterior and posterior regions of the patellar tendon were determined using real-time B-mode ultrasonography at each knee joint angle. Regional strain measures were compared using an automated pixel tracking method. Results: Strain was seen to be greatest for both the anterior and posterior regions with the knee at 900 (7.76 ± 0.89% and 5.06 ± 0.76%). Anterior strain was seen to be significantly greater (p<0.05) than posterior strain for all knee angles apart from 300, 900 = (7.76 vs. 5.06%), 700 = (4.77 vs. 3.75%), and 500 = (3.74 vs. 2.90%). The relative strain (ratio of anterior to posterior), was greatest with the knee joint angle at 900, and decreased as the knee joint angle reduced. Conclusions: The results from this study indicate that not only are there greater absolute tendon strains with the knee in greater flexion, but that the knee joint angle affects the regional strain differentially, resulting in greater shear between the tendon layers with force application when the knee is in greater degrees of flexion. These results have important implications for rehabilitation and training

On the stability of a heated rotating-disk boundary layer in a temperature-dependent viscosity fluid

The paper presents a linear stability analysis of the temperature-dependent boundary-layer flow over a rotating disk. Gas- and liquid-type responses of the viscosity to temperature are considered, and the disk rotates in both a quiescent and an incident axial flow. Temperature-dependent-viscosity flows are typically found to be less stable than the temperature independent cases, with temperature dependences that produce high wall viscosities yielding the least stable flows. Conversely, increasing the incident axial flow strength produces greater flow stability. Transitional Reynolds numbers for these flows are then approximated through an eN-type analysis and are found to vary in approximate concordance with the critical Reynolds number. Examination of the component energy contributions shows that flow stability is affected exclusively through changes to the mean flow. The results are discussed in the context of chemical vapor deposition reactors

Quantifying non-Newtonian effects in rotating boundary-layer flows

© 2016 Elsevier Masson SAS The stability of the boundary-layer on a rotating disk is considered for fluids that adhere to a non-Newtonian governing viscosity relationship. For fluids with shear-rate dependent viscosity the base flow is no longer an exact solution of the Navier–Stokes equations, however, in the limit of large Reynolds number the flow inside the three-dimensional boundary-layer can be determined via a similarity solution. The convective instabilities associated with flows of this nature are described both asymptotically and numerically via separate linear stability analyses. Akin to previous Newtonian studies it is found that there exists two primary modes of instability; the upper-branch type I modes, and the lower-branch type II modes. Results show that both these modes can be stabilised or destabilised depending on the choice of non-Newtonian viscosity model. A number of comments are made regarding the suitability of some of the more well-known non-Newtonian constitutive relationships within the context of the rotating disk model. Such a study is presented with a view to suggesting potential control mechanisms for flows that are practically relevant to the turbo-machinery industry

Vitamin D deficiency prevalence and predictors in early pregnancy among Arab women

Data regarding the prevalence and predictors of vitamin D deficiency during early pregnancy are limited. This study aims to fill this gap. A total of 578 Saudi women in their 1st trimester of pregnancy were recruited between January 2014 and December 2015 from three tertiary care antenatal clinics in Riyadh, Saudi Arabia. Information collected includes socio-economic, anthropometric, and biochemical data, including serum vitamin D (25(OH)D) levels, intake of calcium and vitamin D, physical activity, and sun exposure indices. Pregnant women with 25(OH)D levels 3.5), low HDL-cholesterol, and living in West Riyadh were significant independent predictors for vitamin D deficiency, with odds ratios (ORs) (95% confidence interval) of 25.4 (5.5–117.3), 17.8 (2.3–138.5), 4.0 (1.7–9.5), 3.3 (1.4–7.9), 2.8 (1.2–6.4), and 2.0 (1.1–3.5), respectively. Factors like increased physical activity, sun exposure at noon, sunrise or sunset, high educational status, and residence in North Riyadh were protective against vitamin D deficiency with ORs 0.2 (0.1–0.5); 0.2 (0.1–0.6); 0.3 (0.1–0.9); and 0.4 (0.2–0.8), respectively. All ORs were adjusted for age, BMI, sun exposure, parity, summer season, vitamin D intake, multivitamin intake, physical activity, education, employment, living in the north, and coverage with clothing. In conclusion, the prevalence of vitamin D deficiency among Saudi women during early pregnancy was high (81%). Timely detection and appropriate supplementation with adequate amounts of vitamin D should reduce the risks of vitamin D deficiency and its complications during pregnancy

A systematic review of the data, methods and environmental covariates used to map Aedes-borne arbovirus transmission risk

BACKGROUND: Aedes (Stegomyia)-borne diseases are an expanding global threat, but gaps in surveillance make comprehensive and comparable risk assessments challenging. Geostatistical models combine data from multiple locations and use links with environmental and socioeconomic factors to make predictive risk maps. Here we systematically review past approaches to map risk for different Aedes-borne arboviruses from local to global scales, identifying differences and similarities in the data types, covariates, and modelling approaches used. METHODS: We searched on-line databases for predictive risk mapping studies for dengue, Zika, chikungunya, and yellow fever with no geographical or date restrictions. We included studies that needed to parameterise or fit their model to real-world epidemiological data and make predictions to new spatial locations of some measure of population-level risk of viral transmission (e.g. incidence, occurrence, suitability, etc.). RESULTS: We found a growing number of arbovirus risk mapping studies across all endemic regions and arboviral diseases, with a total of 176 papers published 2002-2022 with the largest increases shortly following major epidemics. Three dominant use cases emerged: (i) global maps to identify limits of transmission, estimate burden and assess impacts of future global change, (ii) regional models used to predict the spread of major epidemics between countries and (iii) national and sub-national models that use local datasets to better understand transmission dynamics to improve outbreak detection and response. Temperature and rainfall were the most popular choice of covariates (included in 50% and 40% of studies respectively) but variables such as human mobility are increasingly being included. Surprisingly, few studies (22%, 31/144) robustly tested combinations of covariates from different domains (e.g. climatic, sociodemographic, ecological, etc.) and only 49% of studies assessed predictive performance via out-of-sample validation procedures. CONCLUSIONS: Here we show that approaches to map risk for different arboviruses have diversified in response to changing use cases, epidemiology and data availability. We identify key differences in mapping approaches between different arboviral diseases, discuss future research needs and outline specific recommendations for future arbovirus mapping

Tandem quadruplication of HMA4 in the zinc (Zn) and cadmium (Cd) hyperaccumulator noccaea caerulescens

Zinc (Zn) and cadmium (Cd) hyperaccumulation may have evolved twice in the Brassicaceae, in Arabidopsis halleri and in the Noccaea genus. Tandem gene duplication and deregulated expression of the Zn transporter, HMA4, has previously been linked to Zn/Cd hyperaccumulation in A. halleri. Here, we tested the hypothesis that tandem duplication and deregulation of HMA4 expression also occurs in Noccaea. A Noccaea caerulescens genomic library was generated, containing 36,864 fosmid pCC1FOSTM clones with insert sizes ~20–40 kbp, and screened with a PCR-generated HMA4 genomic probe. Gene copy number within the genome was estimated through DNA fingerprinting and pooled fosmid pyrosequencing. Gene copy numbers within individual clones was determined by PCR analyses with novel locus specific primers. Entire fosmids were then sequenced individually and reads equivalent to 20-fold coverage were assembled to generate complete whole contigs. Four tandem HMA4 repeats were identified in a contiguous sequence of 101,480 bp based on sequence overlap identities. These were flanked by regions syntenous with up and downstream regions of AtHMA4 in Arabidopsis thaliana. Promoter-reporter b-glucuronidase (GUS) fusion analysis of a NcHMA4 in A. thaliana revealed deregulated expression in roots and shoots, analogous to AhHMA4 promoters, but distinct from AtHMA4 expression which localised to the root vascular tissue. This remarkable consistency in tandem duplication and deregulated expression of metal transport genes between N. caerulescens and A. halleri, which last shared a common ancestor >40 mya, provides intriguing evidence that parallel evolutionary pathways may underlie Zn/Cd hyperaccumulation in Brassicaceae

Image dataset of healthy and infected fig leaves with Ficus leaf worm

This work presents an extensive dataset comprising images meticulously obtained from diverse geographic locations within Iraq, depicting both healthy and infected fig leaves affected by Ficus leafworm. This particular pest poses a significant threat to economic interests, as its infestations often lead to the defoliation of trees, resulting in reduced fruit production. The dataset comprises two distinct classes: infected and healthy, with the acquisition of images executed with precision during the fruiting season, employing state-of-the-art high-resolution equipment, as detailed in the specifications table. In total, the dataset encompasses a substantial 2,321 images, with 1,350 representing infected leaves and 971 depicting healthy ones. The images were acquired through a random sampling approach, ensuring a harmonious blend of balance and diversity across data emanating from distinct fig trees. The proposed dataset carries substantial potential for impact and utility, featuring essential attributes such as the binary classification of infected and healthy leaves. The presented dataset holds the potential to be a valuable resource for the pest control industry within the domains of agriculture and food production

Innate and adaptive autoimmunity in type 1 diabetes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74867/1/j.1399-5448.2007.00334.x.pd

Phenotype and variant spectrum in the LAMB3 form of amelogenesis imperfecta

Amelogenesis imperfecta (AI) is a heterogeneous group of inherited disorders characterized by abnormal formation of dental enamel, either in isolation or as part of a syndrome. Heterozygous variants in laminin subunit beta 3 (LAMB3) cause AI with dominant inheritance in the absence of other cosegregating clinical features. In contrast, biallelic loss-of-function variants in LAMB3 cause recessive junctional epidermolysis bullosa, characterized by life-threatening skin fragility. We identified 2 families segregating autosomal dominant AI with variable degrees of a distinctive hypoplastic phenotype due to pathogenic variants in LAMB3. Whole exome sequencing revealed a nonsense variant (c.3340G>T, p.E1114*) within the final exon in family 1, while Sanger sequencing in family 2 revealed a variant (c.3383-1G>A) in the canonical splice acceptor site of the final exon. Analysis of cDNA from family 2 revealed retention of the final intron leading to a premature termination codon. Two unerupted third molar teeth from individual IV:5 in family 2 were subject to computerized tomography and scanning electron microscopy. LAMB3 molar teeth have a multitude of cusps versus matched controls. LAMB3 enamel was well mineralized but pitted. The architecture of the initially secreted enamel was abnormal, with cervical enamel appearing much less severely affected than coronal enamel. This study further defines the variations in phenotype-genotype correlation for AI due to variants in LAMB3, underlines the clustering of nonsense and frameshift variants causing AI in the absence of junctional epidermolysis bullosa, and highlights the shared AI phenotype arising from variants in genes coding for hemidesmosome proteins